pycom02g12400

BTB POZ domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0006702
Physical Location & Seq
Reverse (-)
8981827 .. 8984386
2560 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g12400.1

Sequence Viewer

Length: 1596 bp
ATGGCTGAAATTAGACTTAAGAGGGTAGAACAAGGCCAAGTCAAGATTAGAAATGTCCCAATTGCCGTGACGCCGGAAGGTTTTTGGTGCTGCCCTTCTCCTGTTGTTTTTCAAAAGAACCTAAAAGCTCACAATTCCATGCACAAACCCAAGCCCTCATCACCACCACCACCACCACCCAAGGAAGCAGCTCACAGAAAACAAACCCCACTGAGCGAGAAAGAGCTAGCCTTTATCCCGTCGAGATCAGCGAGTATTTCTGATGGTCTACGGGGTGTTGGTCCTGAGGCGCCGGATGCGCCGGTGGTTAGTGCATCTATGGTTCCGGAGAGAGTGCCCAGGCCCAAAGTTGAAAATTTGCCTAGGAAGGTAGCAATTGAGTTTGGTGAACCTGGAACTAGTGATATGAAGGTTGTTTTACTTGGAAAGCAGGGATTTTGCGTGAAGTTGAGCGTTCACAAGAATGTTCTGGTTGAGCATAGCAACTTTTTTGCTGATAAACTTTCTGAACAACAGCCTGGTTCAACTTGTCTTAACATGGAAGATTGTGATGATGTTGAAATATATGTTGAAACCGTTGGATTGATGTATTGCAAAGACATGAGGCAACGGCTGATGAAACAAAGTGTCTCGCGTGTACTCCGCATTCTAAAGATTGCAGAACTACTTGGCTTCAACTCGTGCATGCAGTCATGTTTAGAGTACTTGGAAGCAGTCCCGTGGGTTGGGGACGAGGAAGAAGAAAAAGTTGTCTCGTCAGTCTTGCGACTCCAAAGTGAGGGTATTGGGGTCACTCCTGTACTGAAACGAGTCTCGTCAGATGTTTCTAAACCCCAAAAGGACACACTTTCCCACATAATTGGACTTGTTCTTGCAAGCAATGAGGAGAGAGGCAGGCGTGAAATGAAATCCGTAGTGCTCAAGCTCCTTAAGGAGAACAACAGTGCCTCAAGCTCTCAAGGTTCAGCTAACGTCTGCAATGAAACACTGTATAACTCGTGCAGAAGCTGTGTGAACTTGCTGTTGTCCCTATTCAAGCACGCTGCAGAGCCAGAGTCTTCTGATAAACCTGTCGACAACAAAGAACCTGTGGTGAAGCAAATAGCTTTGGGAGCTGATAACCTCTCATGGTTGCTTGAGATTTTGGCTGACAAGCAAGCCGCAGACGAATTTGCAGTAATGTGGGCTAGCCAGCAAGAATTGGCAGCCTTACATACGAAGCTGCCTATTGTGTCTCGTTACCATGTTAGCTGTATCACAGCAAGGCTGTTTGTTGGCATTGGTAGAGGGGAGCTGCTGCCATCTAAGGATACCCGTCTGCTGTTGTTACAAACCTGGCTGCAGCCATTGATTGACGACTACAACTGGCTGCAACATGGGTGCCGGTCATTTGACAGAAAGGTTGTTGAAGAAGGAATTGGTAGGACAATCCTCACTCTGCCTCTAGAGGACCAGCAAAGTATTATGCTTTCTTGGTTGGGAAGTTTTCTCAAGGCTGGCGACAGCTGCCCGAATCTTCAGAGGGCGTTCGAAGTGTGGTGGAGGAGAACTTTCGTTAGACCATACATGGAAGGACAAGGTAGTTCCGCCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB

Protein Analysis

532

Amino Acids

59.13

Weight (kDa)

7.87

Isoelectric Point (pI)

56.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB-POZ_ANK-like PF25553 279 - 520 1.4e-63 BTB/POZ Ankyrin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 289, 1380
AccI GTMKAC 2 cut(s) 268, 1074
AccII CGCG 1 cut(s) 634
AccIII TCCGGA 1 cut(s) 325
AciI CCGC 3 cut(s) 643, 1161, 1587
AcsI RAATTY 2 cut(s) 355, 1169
AcuI CTGAAG 1 cut(s) 1502
AcyI GRCGYC 2 cut(s) 71, 290
AfaI GTAC 3 cut(s) 639, 704, 801
AfiI CCNNNNNNNGG 2 cut(s) 725, 778
AflII CTTAAG 2 cut(s) 17, 929
AgsI TTSAA 8 cut(s) 113, 353, 525, 560, 572, 676, 1036, 1409
AhlI ACTAGT 1 cut(s) 398
AjnI CCWGG 4 cut(s) 338, 391, 517, 1334
AjuI GAANNNNNNNTTGG 2 cut(s) 1401, 1433
Alw21I GWGCWC 1 cut(s) 921
Alw26I GTCTC 4 cut(s) 634, 757, 817, 1239
AlwNI CAGNNNCTG 1 cut(s) 1008
Aor13HI TCCGGA 1 cut(s) 325
AoxI GGCC 2 cut(s) 34, 341
ApoI RAATTY 2 cut(s) 355, 1169
AspA2I CCTAGG 1 cut(s) 362
AspLEI GCGC 2 cut(s) 292, 301
AspS9I GGNCC 3 cut(s) 281, 342, 1450
AsuHPI GGTGA 3 cut(s) 153, 398, 1105
AsuII TTCGAA 1 cut(s) 1530
AsuNHI GCTAGC 2 cut(s) 226, 1187
AvaII GGWCC 2 cut(s) 281, 1450
AvrII CCTAGG 1 cut(s) 362
AxyI CCTNAGG 1 cut(s) 285
BaeGI GKGCMC 1 cut(s) 339
BanI GGYRCC 2 cut(s) 289, 1380
BauI CACGAG 2 cut(s) 679, 997
BbsI GAAGAC 1 cut(s) 1050
Bbv12I GWGCWC 1 cut(s) 921
BccI CCATC 2 cut(s) 257, 1309
BceAI ACGGC 2 cut(s) 50, 626
BciT130I CCWGG 4 cut(s) 340, 393, 519, 1336
BciVI GTATCC 1 cut(s) 1303
BcoDI GTCTC 4 cut(s) 634, 757, 817, 1239
BcuI ACTAGT 1 cut(s) 398
BfaI CTAG 5 cut(s) 227, 363, 399, 1188, 1445
BfmI CTRYAG 2 cut(s) 1044, 1340
BfoI RGCGCY 1 cut(s) 293
BfrI CTTAAG 2 cut(s) 17, 929
BfuI GTATCC 1 cut(s) 1303
BlnI CCTAGG 1 cut(s) 362
BmcAI AGTACT 1 cut(s) 704
Bme1390I CCNGG 4 cut(s) 340, 393, 519, 1336
Bme18I GGWCC 2 cut(s) 281, 1450
BmgT120I GGNCC 3 cut(s) 281, 342, 1450
BmiI GGNNCC 3 cut(s) 291, 324, 1382
BmrFI CCNGG 4 cut(s) 340, 393, 519, 1336
BmrI ACTGGG 1 cut(s) 1585
BmsI GCATC 2 cut(s) 286, 323
BmtI GCTAGC 2 cut(s) 230, 1191
BmuI ACTGGG 1 cut(s) 1585
BpiI GAAGAC 1 cut(s) 1050
Bpu14I TTCGAA 1 cut(s) 1530
BpuEI CTTGAG 5 cut(s) 905, 934, 942, 1157, 1475
BsaHI GRCGYC 2 cut(s) 71, 290
BsaJI CCNNGG 4 cut(s) 180, 338, 362, 719
BsaWI WCCGGW 1 cut(s) 325
Bsc4I CCNNNNNNNGG 2 cut(s) 725, 778
Bse118I RCCGGY 2 cut(s) 301, 1383
Bse1I ACTGG 2 cut(s) 1370, 1591
Bse21I CCTNAGG 1 cut(s) 285
Bse3DI GCAATG 2 cut(s) 886, 985
BseAI TCCGGA 1 cut(s) 325
BseBI CCWGG 4 cut(s) 340, 393, 519, 1336
BseDI CCNNGG 4 cut(s) 180, 338, 362, 719
BseGI GGATG 1 cut(s) 301
BseLI CCNNNNNNNGG 2 cut(s) 725, 778
BseMI GCAATG 2 cut(s) 886, 985
BseMII CTCAG 2 cut(s) 203, 276
BseNI ACTGG 2 cut(s) 1370, 1591
BseRI GAGGAG 2 cut(s) 899, 1558
BseSI GKGCMC 1 cut(s) 339
BsgI GTGCAG 1 cut(s) 1021
Bsh1236I CGCG 1 cut(s) 634
BshFI GGCC 2 cut(s) 36, 343
BshNI GGYRCC 2 cut(s) 289, 1380
BsiHKAI GWGCWC 1 cut(s) 921
BsiSI CCGG 5 cut(s) 74, 293, 302, 326, 1384
BslFI GGGAC 4 cut(s) 41, 701, 743, 1012
BslI CCNNNNNNNGG 2 cut(s) 725, 778
BsmAI GTCTC 4 cut(s) 634, 757, 817, 1239
BsmFI GGGAC 4 cut(s) 41, 701, 743, 1012
BsmI GAATGC 1 cut(s) 645
BsnI GGCC 2 cut(s) 36, 343
Bsp119I TTCGAA 1 cut(s) 1530
Bsp1286I GDGCHC 2 cut(s) 339, 921
Bsp13I TCCGGA 1 cut(s) 325
Bsp143I GATC 1 cut(s) 245
BspACI CCGC 3 cut(s) 643, 1161, 1587
BspANI GGCC 2 cut(s) 36, 343
BspCNI CTCAG 2 cut(s) 204, 277
BspEI TCCGGA 1 cut(s) 325
BspFNI CGCG 1 cut(s) 634
BspLI GGNNCC 3 cut(s) 291, 324, 1382
BspMAI CTGCAG 2 cut(s) 1048, 1344
BspOI GCTAGC 2 cut(s) 230, 1191
BspT104I TTCGAA 1 cut(s) 1530
BspT107I GGYRCC 2 cut(s) 289, 1380
BspTI CTTAAG 2 cut(s) 17, 929
BsrDI GCAATG 2 cut(s) 886, 985
BsrFI RCCGGY 2 cut(s) 301, 1383
BsrI ACTGG 2 cut(s) 1370, 1591
BssAI RCCGGY 2 cut(s) 301, 1383
BssECI CCNNGG 4 cut(s) 180, 338, 362, 719
BssMI GATC 1 cut(s) 245
BssNI GRCGYC 2 cut(s) 71, 290
BssSI CACGAG 2 cut(s) 679, 997
BssT1I CCWWGG 2 cut(s) 180, 362
Bst2BI CACGAG 2 cut(s) 679, 997
Bst2UI CCWGG 4 cut(s) 340, 393, 519, 1336
Bst4CI ACNGT 3 cut(s) 577, 944, 990
BstACI GRCGYC 2 cut(s) 71, 290
BstAFI CTTAAG 2 cut(s) 17, 929
BstBI TTCGAA 1 cut(s) 1530
BstC8I GCNNGC 9 cut(s) 228, 686, 877, 896, 1041, 1158, 1189, 1193, 1498
BstDEI CTNAG 3 cut(s) 212, 285, 1305
BstDSI CCRYGG 1 cut(s) 719
BstF5I GGATG 1 cut(s) 301
BstFNI CGCG 1 cut(s) 634
BstH2I RGCGCY 1 cut(s) 293
BstHHI GCGC 2 cut(s) 292, 301
BstKTI GATC 1 cut(s) 248
BstMAI GTCTC 4 cut(s) 634, 757, 817, 1239
BstMBI GATC 1 cut(s) 245
BstMWI GCNNNNNNNGC 4 cut(s) 296, 298, 1112, 1506
BstNI CCWGG 4 cut(s) 340, 393, 519, 1336
BstNSI RCATGY 1 cut(s) 688
BstSCI CCNGG 4 cut(s) 338, 391, 517, 1334
BstSFI CTRYAG 2 cut(s) 1044, 1340
BstSLI GKGCMC 1 cut(s) 339
BstUI CGCG 1 cut(s) 634
BstV2I GAAGAC 1 cut(s) 1050
BstXI CCANNNNNNTGG 1 cut(s) 860
Bsu36I CCTNAGG 1 cut(s) 285
BsuI GTATCC 1 cut(s) 1303
BsuRI GGCC 2 cut(s) 36, 343
BtgI CCRYGG 1 cut(s) 719
BtsCI GGATG 1 cut(s) 301
BtsIMutI CAGTG 3 cut(s) 209, 949, 986
Cac8I GCNNGC 9 cut(s) 228, 686, 877, 896, 1041, 1158, 1189, 1193, 1498
CaiI CAGNNNCTG 1 cut(s) 1008
CfoI GCGC 2 cut(s) 292, 301
Cfr10I RCCGGY 2 cut(s) 301, 1383
Cfr13I GGNCC 3 cut(s) 281, 342, 1450
CseI GACGC 1 cut(s) 79
Csp6I GTAC 3 cut(s) 638, 703, 800
CviAII CATG 9 cut(s) 139, 538, 601, 685, 693, 1128, 1244, 1376, 1567
CviQI GTAC 3 cut(s) 638, 703, 800
DdeI CTNAG 3 cut(s) 212, 285, 1305
DinI GGCGCC 1 cut(s) 291
DpnI GATC 1 cut(s) 247
DpnII GATC 1 cut(s) 245
EciI GGCGGA 1 cut(s) 1576
Eco130I CCWWGG 2 cut(s) 180, 362
Eco47I GGWCC 2 cut(s) 281, 1450
Eco57I CTGAAG 1 cut(s) 1502
Eco81I CCTNAGG 1 cut(s) 285
EcoRII CCWGG 4 cut(s) 338, 391, 517, 1334
EcoT14I CCWWGG 2 cut(s) 180, 362
EgeI GGCGCC 1 cut(s) 291
EheI GGCGCC 1 cut(s) 291
ErhI CCWWGG 2 cut(s) 180, 362
FaeI CATG 9 cut(s) 142, 541, 604, 688, 696, 1131, 1247, 1379, 1570
FaqI GGGAC 4 cut(s) 41, 701, 743, 1012
FatI CATG 9 cut(s) 138, 537, 600, 684, 692, 1127, 1243, 1375, 1566
FblI GTMKAC 2 cut(s) 268, 1074
FokI GGATG 1 cut(s) 308
FspBI CTAG 5 cut(s) 227, 363, 399, 1188, 1445
GlaI GCGC 2 cut(s) 291, 300
HaeII RGCGCY 1 cut(s) 293
HaeIII GGCC 2 cut(s) 36, 343
HapII CCGG 5 cut(s) 74, 293, 302, 326, 1384
HgaI GACGC 1 cut(s) 79
HhaI GCGC 2 cut(s) 292, 301
Hin1I GRCGYC 2 cut(s) 71, 290
Hin1II CATG 9 cut(s) 142, 541, 604, 688, 696, 1131, 1247, 1379, 1570
Hin6I GCGC 2 cut(s) 290, 299
HinP1I GCGC 2 cut(s) 290, 299
HincII GTYRAC 1 cut(s) 1075
HindII GTYRAC 1 cut(s) 1075
HinfI GANTC 4 cut(s) 768, 810, 1055, 1513
HpaII CCGG 5 cut(s) 74, 293, 302, 326, 1384
HphI GGTGA 3 cut(s) 153, 398, 1105
Hpy166II GTNNAC 6 cut(s) 269, 389, 457, 638, 1015, 1075
Hpy188I TCNGA 5 cut(s) 262, 508, 820, 1063, 1521
Hpy188III TCNNGA 5 cut(s) 43, 243, 284, 326, 1445
Hpy8I GTNNAC 6 cut(s) 269, 389, 457, 638, 1015, 1075
Hpy99I CGWCG 1 cut(s) 244
HpyAV CCTTC 6 cut(s) 71, 105, 361, 403, 1406, 1565
HpyCH4III ACNGT 3 cut(s) 577, 944, 990
HpyCH4IV ACGT 1 cut(s) 972
HpyF10VI GCNNNNNNNGC 4 cut(s) 296, 298, 1112, 1506
HpyF3I CTNAG 3 cut(s) 212, 285, 1305
HpySE526I ACGT 1 cut(s) 972
Hsp92I GRCGYC 2 cut(s) 71, 290
Hsp92II CATG 9 cut(s) 142, 541, 604, 688, 696, 1131, 1247, 1379, 1570
HspAI GCGC 2 cut(s) 290, 299
KasI GGCGCC 1 cut(s) 289
Kpn2I TCCGGA 1 cut(s) 325
Kzo9I GATC 1 cut(s) 245
LmnI GCTCC 3 cut(s) 930, 1112, 1291
LweI GCATC 2 cut(s) 286, 323
MaeI CTAG 5 cut(s) 227, 363, 399, 1188, 1445
MaeII ACGT 1 cut(s) 972
MaeIII GTNAC 4 cut(s) 67, 790, 1238, 1326
MalI GATC 1 cut(s) 247
MboI GATC 1 cut(s) 245
MboII GAAGA 6 cut(s) 554, 749, 752, 1050, 1421, 1508
MfeI CAATTG 2 cut(s) 60, 375
MhlI GDGCHC 2 cut(s) 339, 921
MluCI AATT 9 cut(s) 9, 60, 133, 355, 375, 858, 1169, 1199, 1416
Mly113I GGCGCC 1 cut(s) 290
MlyI GAGTC 3 cut(s) 762, 819, 1064
MmeI TCCRAC 1 cut(s) 559
MroI TCCGGA 1 cut(s) 325
MseI TTAA 3 cut(s) 18, 534, 930
MslI CAYNNNNRTG 1 cut(s) 462
MspA1I CMGCKG 1 cut(s) 1506
MspCI CTTAAG 2 cut(s) 17, 929
MspI CCGG 5 cut(s) 74, 293, 302, 326, 1384
MspR9I CCNGG 4 cut(s) 340, 393, 519, 1336
MunI CAATTG 2 cut(s) 60, 375
Mva1269I GAATGC 1 cut(s) 645
MvaI CCWGG 4 cut(s) 340, 393, 519, 1336
MvnI CGCG 1 cut(s) 634
MwoI GCNNNNNNNGC 4 cut(s) 296, 298, 1112, 1506
NarI GGCGCC 1 cut(s) 290
NdeII GATC 1 cut(s) 245
NheI GCTAGC 2 cut(s) 226, 1187
NlaIII CATG 9 cut(s) 142, 541, 604, 688, 696, 1131, 1247, 1379, 1570
NlaIV GGNNCC 3 cut(s) 291, 324, 1382
NmuCI GTSAC 2 cut(s) 67, 790
NspI RCATGY 1 cut(s) 688
NspV TTCGAA 1 cut(s) 1530
PaeI GCATGC 1 cut(s) 688
PcsI WCGNNNNNNNCGW 1 cut(s) 248
PctI GAATGC 1 cut(s) 645
PfeI GAWTC 1 cut(s) 1513
PleI GAGTC 3 cut(s) 762, 818, 1063
PluTI GGCGCC 1 cut(s) 293
PpsI GAGTC 3 cut(s) 762, 818, 1063
Psp6I CCWGG 4 cut(s) 338, 391, 517, 1334
PspGI CCWGG 4 cut(s) 338, 391, 517, 1334
PspN4I GGNNCC 3 cut(s) 291, 324, 1382
PspPI GGNCC 3 cut(s) 281, 342, 1450
PstI CTGCAG 2 cut(s) 1048, 1344
PstNI CAGNNNCTG 1 cut(s) 1008
PvuII CAGCTG 1 cut(s) 1506
RsaI GTAC 3 cut(s) 639, 704, 801
RsaNI GTAC 3 cut(s) 638, 703, 800
RseI CAYNNNNRTG 1 cut(s) 462
SalI GTCGAC 1 cut(s) 1073
SaqAI TTAA 3 cut(s) 18, 534, 930
Sau3AI GATC 1 cut(s) 245
Sau96I GGNCC 3 cut(s) 281, 342, 1450
ScaI AGTACT 1 cut(s) 704
SchI GAGTC 3 cut(s) 762, 819, 1064
ScrFI CCNGG 4 cut(s) 340, 393, 519, 1336
SduI GDGCHC 2 cut(s) 339, 921
SfaNI GCATC 2 cut(s) 286, 323
SfcI CTRYAG 2 cut(s) 1044, 1340
SfoI GGCGCC 1 cut(s) 291
SfuI TTCGAA 1 cut(s) 1530
SgrAI CRCCGGYG 1 cut(s) 301
SinI GGWCC 2 cut(s) 281, 1450
SmiMI CAYNNNNRTG 1 cut(s) 462
SmlI CTYRAG 7 cut(s) 17, 920, 929, 949, 957, 1136, 1490
SmoI CTYRAG 7 cut(s) 17, 920, 929, 949, 957, 1136, 1490
SpeI ACTAGT 1 cut(s) 398
SphI GCATGC 1 cut(s) 688
Sse9I AATT 9 cut(s) 9, 60, 133, 355, 375, 858, 1169, 1199, 1416
SsiI CCGC 3 cut(s) 643, 1161, 1587
SspDI GGCGCC 1 cut(s) 289
SspMI CTAG 5 cut(s) 227, 363, 399, 1188, 1445
StyD4I CCNGG 4 cut(s) 338, 391, 517, 1334
StyI CCWWGG 2 cut(s) 180, 362
TaaI ACNGT 3 cut(s) 577, 944, 990
TaiI ACGT 1 cut(s) 975
TaqI TCGA 3 cut(s) 242, 1074, 1530
TasI AATT 9 cut(s) 9, 60, 133, 355, 375, 858, 1169, 1199, 1416
TatI WGTACW 3 cut(s) 637, 702, 799
TauI GCSGC 1 cut(s) 1163
TfiI GAWTC 1 cut(s) 1513
Tru1I TTAA 3 cut(s) 18, 534, 930
Tru9I TTAA 3 cut(s) 18, 534, 930
TscAI CASTG 3 cut(s) 216, 949, 993
TseFI GTSAC 2 cut(s) 67, 790
Tsp45I GTSAC 2 cut(s) 67, 790
TspDTI ATGAA 4 cut(s) 422, 632, 920, 996
TspGWI ACGGA 1 cut(s) 901
TspRI CASTG 3 cut(s) 216, 949, 993
Vha464I CTTAAG 2 cut(s) 17, 929
VpaK11BI GGWCC 2 cut(s) 281, 1450
XapI RAATTY 2 cut(s) 355, 1169
XbaI TCTAGA 1 cut(s) 1444
XceI RCATGY 1 cut(s) 688
XmaJI CCTAGG 1 cut(s) 362
XmiI GTMKAC 2 cut(s) 268, 1074
XspI CTAG 5 cut(s) 227, 363, 399, 1188, 1445
ZrmI AGTACT 1 cut(s) 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.