MD15G1292600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016139
Physical Location & Seq
Reverse (-)
27103197 .. 27104384
1188 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1292600.v1.1.491

Sequence Viewer

Length: 1188 bp
ATGGCAGCAGTTCCAAACATTGCTGCTGATGATGAACAAGAAAGAGTACTCCTTGACGAAGATTTTGCCTCCGACGCTGACGAATTTGAGGAAGAACAAGAAGAAGATGAAGAGGAAGAAGTGTCACTTTGTGATCTTCCAGTGAAATTGATCAAACAGGATCAAACACAATTTTCCAATCTCAATCTCAAATCTGAGTCTTCCCATGTCGCCATTGAAAAGCAAGATCAAGAATTTGATTTCTGTTCGTCGATGCGTGGCGGGTTGAATAATTTGTTGGCAGACACAAAAATGTGTGCTGCAGATGAAGTGTTCTTCCAAGGCCAAATTCTTCCTCTCCGCCTCTCCATCAGCTCAGATACCCGCTCCAACAGACCAACCAGGCAGTGTCCGTTGAGCGGTGTCGGGCTCAGAAGCAGCAGTTGTAGAAGCAGAATTAGTCACAACTCATCATTCAGCAGCAGCACCAGCTCCACCACCACAACAGGCATTGCTTCAAAGCCAAGAGCAGCAAGACCAAGAAACCAATTCTACACAGAACCAAGTCCAAAACCGCAAATCAAAATTCCGAATTCTCGATTAGAAAAGGCGGGCAGCCGGCATCATAAGTCCTCGATGTGGGATTTTTTCCGGTTGGGTTTGGTGCGCACTCCCGACATGGAATTGCAGGATTTTAACAAGGTACTTCGCAGCAATAGTAGTACTAGTACTCGCGCGAATAACAACGCGAGAAATTCCGTCAGTCGAAATAGCAGTGTTAGTAGCACCAACAGTACAAGTTTCATATCCAGTTACAACAATGCTGCTAGTGCTATTAGTGGTTTGAAGTCGGATAATTTGAAACAGAAGAAGCAGAGCTTTTTTAGCAGTGTTCATGGCTGCAAGTGCTCGATCGAAATGGTGGCGTCTGATAACGTGATTTTCAAGAGCCGATCGAGCAGCGGTAGGAGTAGTAGTGCAAGTCACATCAATGAAGGTGGTGGTGTAAGAGCGGCAGCAGCTGATTCGATGCATGCCGCGTCGGAAGAAAAAGTAGCGATAGAGTTGAAGATGATGACGAGGAAGAATAACAAGAAGCAGCAGCAAAGTTGTAAACATAAGGAAAAGCAGGCCCTTTCACATCACAGAACGTATGAATGGCTAAGGCAGCACTCGCATGCAAACTCTCCTTTTCCTGTGCATGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

396

Amino Acids

43.79

Weight (kDa)

8.55

Isoelectric Point (pI)

69.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016139)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67350
fragaria_vesca FvH4_1g17130
malus_domestica MD02G1181700.v1.1 MD15G1292600.v1.1
prunus_persica Prupe.6G224800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0107301
rosa_laevigata RLG00000017569
rosa_multiflora Rmu_sc0006416.1_g000002
rosa_roxburghii Rroxscaffold_2G00136450
rosa_rugosa Rorug02G0141700
rosa_samantha Rh2AG192600 Rh2BG204500 Rh2CG197800 Rh2DG199800
rosa_wichuraiana Rw2G015240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 647
AccBSI CCGCTC 3 cut(s) 366, 399, 992
AccII CGCG 4 cut(s) 714, 716, 728, 1019
AciI CCGC 9 cut(s) 261, 340, 364, 399, 554, 590, 942, 992, 1017
AclWI GGATC 1 cut(s) 168
AcsI RAATTY 6 cut(s) 83, 233, 327, 564, 571, 733
AcyI GRCGYC 1 cut(s) 905
AdeI CACNNNGTG 1 cut(s) 131
AfaI GTAC 5 cut(s) 48, 684, 703, 709, 775
AfiI CCNNNNNNNGG 2 cut(s) 398, 618
AgsI TTSAA 7 cut(s) 218, 268, 498, 826, 841, 925, 1048
AhlI ACTAGT 1 cut(s) 704
AjnI CCWGG 1 cut(s) 380
AjuI GAANNNNNNNTTGG 4 cut(s) 260, 292, 312, 344
AluBI AGCT 4 cut(s) 354, 471, 858, 1001
AluI AGCT 4 cut(s) 354, 471, 858, 1001
Alw21I GWGCWC 1 cut(s) 890
AlwI GGATC 1 cut(s) 168
AlwNI CAGNNNCTG 1 cut(s) 1001
AoxI GGCC 2 cut(s) 322, 1110
ApoI RAATTY 6 cut(s) 83, 233, 327, 564, 571, 733
ArsI GACNNNNNNTTYG 2 cut(s) 47, 79
AspLEI GCGC 2 cut(s) 648, 716
AspS9I GGNCC 1 cut(s) 1111
BanII GRGCYC 1 cut(s) 411
BbsI GAAGAC 1 cut(s) 192
Bbv12I GWGCWC 1 cut(s) 890
BccI CCATC 1 cut(s) 356
BcgI CGANNNNNNTGC 4 cut(s) 47, 81, 987, 1021
BciT130I CCWGG 1 cut(s) 382
BclI TGATCA 1 cut(s) 150
BcuI ACTAGT 1 cut(s) 704
BfaI CTAG 2 cut(s) 705, 807
BfmI CTRYAG 1 cut(s) 300
BmcAI AGTACT 3 cut(s) 48, 703, 709
Bme1390I CCNGG 1 cut(s) 382
BmgT120I GGNCC 1 cut(s) 1111
BmrFI CCNGG 1 cut(s) 382
BmsI GCATC 3 cut(s) 243, 610, 999
BpiI GAAGAC 1 cut(s) 192
Bpu10I CCTNAGC 1 cut(s) 1142
BsaHI GRCGYC 1 cut(s) 905
BsaJI CCNNGG 1 cut(s) 319
BsaWI WCCGGW 1 cut(s) 630
BsaXI ACNNNNNCTCC 2 cut(s) 940, 970
Bsc4I CCNNNNNNNGG 2 cut(s) 398, 618
Bse118I RCCGGY 1 cut(s) 597
Bse1I ACTGG 2 cut(s) 140, 789
Bse3DI GCAATG 2 cut(s) 18, 489
BseBI CCWGG 1 cut(s) 382
BseDI CCNNGG 1 cut(s) 319
BseLI CCNNNNNNNGG 2 cut(s) 398, 618
BseMI GCAATG 2 cut(s) 18, 489
BseMII CTCAG 3 cut(s) 186, 369, 424
BseNI ACTGG 2 cut(s) 140, 789
Bsh1236I CGCG 4 cut(s) 714, 716, 728, 1019
Bsh1285I CGRYCG 2 cut(s) 894, 935
BshFI GGCC 2 cut(s) 324, 1112
BsiEI CGRYCG 2 cut(s) 894, 935
BsiHKAI GWGCWC 1 cut(s) 890
BsiSI CCGG 2 cut(s) 598, 631
BslI CCNNNNNNNGG 2 cut(s) 398, 618
BsnI GGCC 2 cut(s) 324, 1112
Bsp1286I GDGCHC 2 cut(s) 411, 890
Bsp143I GATC 6 cut(s) 133, 150, 160, 226, 891, 932
BspACI CCGC 9 cut(s) 261, 340, 364, 399, 554, 590, 942, 992, 1017
BspANI GGCC 2 cut(s) 324, 1112
BspCNI CTCAG 3 cut(s) 187, 368, 423
BspFNI CGCG 4 cut(s) 714, 716, 728, 1019
BspMAI CTGCAG 1 cut(s) 304
BspPI GGATC 1 cut(s) 168
BsrBI CCGCTC 3 cut(s) 366, 399, 992
BsrDI GCAATG 2 cut(s) 18, 489
BsrFI RCCGGY 1 cut(s) 597
BsrI ACTGG 2 cut(s) 140, 789
BssAI RCCGGY 1 cut(s) 597
BssECI CCNNGG 1 cut(s) 319
BssMI GATC 6 cut(s) 133, 150, 160, 226, 891, 932
BssNI GRCGYC 1 cut(s) 905
BssT1I CCWWGG 1 cut(s) 319
Bst2UI CCWGG 1 cut(s) 382
Bst4CI ACNGT 1 cut(s) 773
Bst6I CTCTTC 1 cut(s) 105
BstACI GRCGYC 1 cut(s) 905
BstC8I GCNNGC 5 cut(s) 592, 599, 1014, 1110, 1158
BstDEI CTNAG 4 cut(s) 195, 355, 410, 1142
BstFNI CGCG 4 cut(s) 714, 716, 728, 1019
BstHHI GCGC 2 cut(s) 648, 716
BstKTI GATC 6 cut(s) 136, 153, 163, 229, 894, 935
BstMBI GATC 6 cut(s) 133, 150, 160, 226, 891, 932
BstMCI CGRYCG 2 cut(s) 894, 935
BstMWI GCNNNNNNNGC 9 cut(s) 74, 468, 809, 864, 885, 936, 998, 1147, 1153
BstNI CCWGG 1 cut(s) 382
BstNSI RCATGY 3 cut(s) 1016, 1160, 1184
BstSCI CCNGG 1 cut(s) 380
BstSFI CTRYAG 1 cut(s) 300
BstUI CGCG 4 cut(s) 714, 716, 728, 1019
BstV2I GAAGAC 1 cut(s) 192
BsuRI GGCC 2 cut(s) 324, 1112
BtsI GCAGTG 3 cut(s) 392, 760, 874
BtsIMutI CAGTG 4 cut(s) 147, 392, 760, 874
Cac8I GCNNGC 5 cut(s) 592, 599, 1014, 1110, 1158
CaiI CAGNNNCTG 1 cut(s) 1001
CfoI GCGC 2 cut(s) 648, 716
Cfr10I RCCGGY 1 cut(s) 597
Cfr13I GGNCC 1 cut(s) 1111
CseI GACGC 3 cut(s) 83, 894, 1008
Csp6I GTAC 5 cut(s) 47, 683, 702, 708, 774
CspCI CAANNNNNGTGG 2 cut(s) 958, 993
CviAII CATG 6 cut(s) 206, 658, 875, 1013, 1157, 1181
CviQI GTAC 5 cut(s) 47, 683, 702, 708, 774
DdeI CTNAG 4 cut(s) 195, 355, 410, 1142
DpnI GATC 6 cut(s) 135, 152, 162, 228, 893, 934
DpnII GATC 6 cut(s) 133, 150, 160, 226, 891, 932
DraIII CACNNNGTG 1 cut(s) 131
Eam1104I CTCTTC 1 cut(s) 105
EarI CTCTTC 1 cut(s) 105
EciI GGCGGA 1 cut(s) 329
Eco130I CCWWGG 1 cut(s) 319
Eco24I GRGCYC 1 cut(s) 411
EcoO109I RGGNCCY 1 cut(s) 1111
EcoRI GAATTC 1 cut(s) 571
EcoRII CCWGG 1 cut(s) 380
EcoT14I CCWWGG 1 cut(s) 319
EcoT22I ATGCAT 1 cut(s) 1014
EcoT38I GRGCYC 1 cut(s) 411
ErhI CCWWGG 1 cut(s) 319
FaeI CATG 6 cut(s) 209, 661, 878, 1016, 1160, 1184
FalI AAGNNNNNCTT 4 cut(s) 111, 143, 842, 874
FatI CATG 6 cut(s) 205, 657, 874, 1012, 1156, 1180
FauI CCCGC 3 cut(s) 254, 371, 583
FbaI TGATCA 1 cut(s) 150
FriOI GRGCYC 1 cut(s) 411
FspAI RTGCGCAY 1 cut(s) 647
FspBI CTAG 2 cut(s) 705, 807
FspI TGCGCA 1 cut(s) 647
GlaI GCGC 2 cut(s) 647, 715
HaeIII GGCC 2 cut(s) 324, 1112
HapII CCGG 2 cut(s) 598, 631
HgaI GACGC 3 cut(s) 83, 894, 1008
HhaI GCGC 2 cut(s) 648, 716
Hin1I GRCGYC 1 cut(s) 905
Hin1II CATG 6 cut(s) 209, 661, 878, 1016, 1160, 1184
Hin6I GCGC 2 cut(s) 646, 714
HinP1I GCGC 2 cut(s) 646, 714
HinfI GANTC 2 cut(s) 197, 1004
HpaII CCGG 2 cut(s) 598, 631
Hpy166II GTNNAC 1 cut(s) 1094
Hpy188I TCNGA 8 cut(s) 73, 196, 358, 413, 570, 832, 910, 1024
Hpy188III TCNNGA 4 cut(s) 230, 576, 653, 925
Hpy8I GTNNAC 1 cut(s) 1094
Hpy99I CGWCG 3 cut(s) 77, 253, 1024
HpyAV CCTTC 1 cut(s) 968
HpyCH4III ACNGT 1 cut(s) 773
HpyCH4IV ACGT 2 cut(s) 915, 1130
HpyCH4V TGCA 7 cut(s) 302, 667, 882, 959, 1012, 1160, 1180
HpyF10VI GCNNNNNNNGC 9 cut(s) 74, 468, 809, 864, 885, 936, 998, 1147, 1153
HpyF3I CTNAG 4 cut(s) 195, 355, 410, 1142
HpySE526I ACGT 2 cut(s) 915, 1130
Hsp92I GRCGYC 1 cut(s) 905
Hsp92II CATG 6 cut(s) 209, 661, 878, 1016, 1160, 1184
HspAI GCGC 2 cut(s) 646, 714
KroI GCCGGC 1 cut(s) 597
KroNI GCCGGC 1 cut(s) 599
Ksp22I TGATCA 1 cut(s) 150
Kzo9I GATC 6 cut(s) 133, 150, 160, 226, 891, 932
LmnI GCTCC 2 cut(s) 371, 476
LweI GCATC 3 cut(s) 243, 610, 999
MaeI CTAG 2 cut(s) 705, 807
MaeII ACGT 2 cut(s) 915, 1130
MaeIII GTNAC 4 cut(s) 123, 440, 791, 962
MalI GATC 6 cut(s) 135, 152, 162, 228, 893, 934
MbiI CCGCTC 3 cut(s) 366, 399, 992
MboI GATC 6 cut(s) 133, 150, 160, 226, 891, 932
MhlI GDGCHC 2 cut(s) 411, 890
MlyI GAGTC 1 cut(s) 206
MmeI TCCRAC 4 cut(s) 96, 393, 810, 1002
MnlI CCTC 7 cut(s) 79, 82, 106, 345, 353, 622, 1053
Mph1103I ATGCAT 1 cut(s) 1014
MroNI GCCGGC 1 cut(s) 597
MseI TTAA 2 cut(s) 675, 1186
MslI CAYNNNNRTG 3 cut(s) 290, 969, 1155
MspA1I CMGCKG 2 cut(s) 942, 1001
MspI CCGG 2 cut(s) 598, 631
MspR9I CCNGG 1 cut(s) 382
MvaI CCWGG 1 cut(s) 382
MvnI CGCG 4 cut(s) 714, 716, 728, 1019
MwoI GCNNNNNNNGC 9 cut(s) 74, 468, 809, 864, 885, 936, 998, 1147, 1153
NaeI GCCGGC 1 cut(s) 599
NdeII GATC 6 cut(s) 133, 150, 160, 226, 891, 932
NgoMIV GCCGGC 1 cut(s) 597
NlaIII CATG 6 cut(s) 209, 661, 878, 1016, 1160, 1184
NmuCI GTSAC 3 cut(s) 123, 440, 962
NsbI TGCGCA 1 cut(s) 647
NsiI ATGCAT 1 cut(s) 1014
NspI RCATGY 3 cut(s) 1016, 1160, 1184
PaeI GCATGC 2 cut(s) 1016, 1160
PdiI GCCGGC 1 cut(s) 599
PfeI GAWTC 1 cut(s) 1004
Ple19I CGATCG 2 cut(s) 894, 935
PleI GAGTC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 205
Psp6I CCWGG 1 cut(s) 380
PspGI CCWGG 1 cut(s) 380
PspPI GGNCC 1 cut(s) 1111
PstI CTGCAG 1 cut(s) 304
PstNI CAGNNNCTG 1 cut(s) 1001
PvuI CGATCG 2 cut(s) 894, 935
PvuII CAGCTG 1 cut(s) 1001
RsaI GTAC 5 cut(s) 48, 684, 703, 709, 775
RsaNI GTAC 5 cut(s) 47, 683, 702, 708, 774
RseI CAYNNNNRTG 3 cut(s) 290, 969, 1155
SaqAI TTAA 2 cut(s) 675, 1186
Sau3AI GATC 6 cut(s) 133, 150, 160, 226, 891, 932
Sau96I GGNCC 1 cut(s) 1111
ScaI AGTACT 3 cut(s) 48, 703, 709
SchI GAGTC 1 cut(s) 206
ScrFI CCNGG 1 cut(s) 382
SduI GDGCHC 2 cut(s) 411, 890
SetI ASST 8 cut(s) 356, 473, 684, 860, 918, 979, 1003, 1133
SfaNI GCATC 3 cut(s) 243, 610, 999
SfcI CTRYAG 1 cut(s) 300
SmiMI CAYNNNNRTG 3 cut(s) 290, 969, 1155
SpeI ACTAGT 1 cut(s) 704
SphI GCATGC 2 cut(s) 1016, 1160
SsiI CCGC 9 cut(s) 261, 340, 364, 399, 554, 590, 942, 992, 1017
SspMI CTAG 2 cut(s) 705, 807
StyD4I CCNGG 1 cut(s) 380
StyI CCWWGG 1 cut(s) 319
TaaI ACNGT 1 cut(s) 773
TaiI ACGT 2 cut(s) 918, 1133
TaqI TCGA 8 cut(s) 251, 577, 614, 745, 890, 894, 935, 1007
TatI WGTACW 4 cut(s) 46, 701, 707, 773
TauI GCSGC 2 cut(s) 995, 1019
TfiI GAWTC 1 cut(s) 1004
Tru1I TTAA 2 cut(s) 675, 1186
Tru9I TTAA 2 cut(s) 675, 1186
TscAI CASTG 4 cut(s) 147, 392, 760, 874
TseFI GTSAC 3 cut(s) 123, 440, 962
Tsp45I GTSAC 3 cut(s) 123, 440, 962
TspDTI ATGAA 7 cut(s) 48, 123, 321, 772, 863, 987, 1149
TspGWI ACGGA 2 cut(s) 381, 727
TspRI CASTG 4 cut(s) 147, 392, 760, 874
XapI RAATTY 6 cut(s) 83, 233, 327, 564, 571, 733
XceI RCATGY 3 cut(s) 1016, 1160, 1184
XspI CTAG 2 cut(s) 705, 807
ZrmI AGTACT 3 cut(s) 48, 703, 709
Zsp2I ATGCAT 1 cut(s) 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.