MD15G1327700.v1.1

Protein DOWNSTREAM OF

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016738
Physical Location & Seq
Reverse (-)
35443531 .. 35444507
977 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1327700.v1.1.491

Sequence Viewer

Length: 489 bp
ATGGCCATGTCTAGGGTGGTGGTGTTGTTGGCTCTGTGCATGTTTCTGGGGCTTGTTAGCGCACGCACGTTGGGCAATCCCTTCCACGTTCAAGGTCGCGTCTACTGTGACACTTGCCGATGCGGTTTTGAGACGACTGCCACGAATTACATTCCTGGTGCAAGGGTGAGAATTGAGTGCAAAGACAGGAACACCTTGCAGCTTGCATACAGCGTCGAGGGAGAGACTGATTCGACTGGAACATACAACATTTTGGTTGAAGAGGACCATGAAGACCAAATTTGTGAGTCTGTCCTTGTTAGCAGTCCCATAAGTGACTGTAAATCGGCAGACCCCGGACGCAGCCGTGCGAACGTTGTAGTCACCCGTTACAACGGTGTGGTTAAGGACAAGCGTTATGCAAATAACATGGGCTTCTTCAAGAACGAGCCATTGGCTGGTTGTGAAAAAATCCTAGAGCAATACAAGAACTACGATCAGCAGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

163

Amino Acids

18.12

Weight (kDa)

5.48

Isoelectric Point (pI)

44.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 30 - 111 3e-18 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016738)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21330
malus_domestica MD15G1327700.v1.1 MD15G1327800.v1.1
prunus_persica Prupe.6G181300_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0115161
rosa_laevigata RLG00000018163
rosa_multiflora Rmu_sc0002784.1_g000019
rosa_roxburghii Rroxscaffold_2G00130640
rosa_rugosa Rorug02G0194100
rosa_samantha Rh2AG252600 Rh2BG262200 Rh2CG257100 Rh2DG260800
rosa_wichuraiana Rw2G019700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 437
AccI GTMKAC 1 cut(s) 102
AccII CGCG 1 cut(s) 99
AciI CCGC 1 cut(s) 123
AclI AACGTT 1 cut(s) 354
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 279
AfiI CCNNNNNNNGG 2 cut(s) 12, 437
AgsI TTSAA 3 cut(s) 92, 260, 421
AjnI CCWGG 1 cut(s) 154
AjuI GAANNNNNNNTTGG 2 cut(s) 416, 448
AluBI AGCT 1 cut(s) 202
AluI AGCT 1 cut(s) 202
Alw26I GTCTC 2 cut(s) 125, 218
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 199, 342
ApoI RAATTY 1 cut(s) 279
AspLEI GCGC 1 cut(s) 62
AspS9I GGNCC 1 cut(s) 265
AsuC2I CCSGG 1 cut(s) 336
AsuHPI GGTGA 2 cut(s) 178, 355
AvaII GGWCC 1 cut(s) 265
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 279
BbvI GCAGC 2 cut(s) 211, 354
BceAI ACGGC 1 cut(s) 330
BciT130I CCWGG 1 cut(s) 156
BcnI CCSGG 1 cut(s) 336
BcoDI GTCTC 2 cut(s) 125, 218
BfaI CTAG 2 cut(s) 12, 455
BisI GCNGC 2 cut(s) 200, 343
BlsI GCNGC 2 cut(s) 201, 344
Bme1390I CCNGG 2 cut(s) 156, 336
Bme18I GGWCC 1 cut(s) 265
BmgT120I GGNCC 1 cut(s) 265
BmrFI CCNGG 2 cut(s) 156, 336
BmsI GCATC 1 cut(s) 110
BpiI GAAGAC 1 cut(s) 279
BpuMI CCSGG 1 cut(s) 336
BsaJI CCNNGG 1 cut(s) 334
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 437
Bse1I ACTGG 1 cut(s) 241
BseBI CCWGG 1 cut(s) 156
BseDI CCNNGG 1 cut(s) 334
BseLI CCNNNNNNNGG 2 cut(s) 12, 437
BseNI ACTGG 1 cut(s) 241
BseXI GCAGC 2 cut(s) 211, 354
Bsh1236I CGCG 1 cut(s) 99
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 1 cut(s) 336
BslFI GGGAC 1 cut(s) 291
BslI CCNNNNNNNGG 2 cut(s) 12, 437
BsmAI GTCTC 2 cut(s) 125, 218
BsmBI CGTCTC 1 cut(s) 125
BsmFI GGGAC 1 cut(s) 291
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 1 cut(s) 475
BspACI CCGC 1 cut(s) 123
BspANI GGCC 1 cut(s) 5
BspFNI CGCG 1 cut(s) 99
BsrI ACTGG 1 cut(s) 241
BssECI CCNNGG 1 cut(s) 334
BssMI GATC 1 cut(s) 475
Bst2UI CCWGG 1 cut(s) 156
Bst4CI ACNGT 3 cut(s) 107, 320, 377
Bst6I CTCTTC 1 cut(s) 255
BstC8I GCNNGC 2 cut(s) 64, 204
BstFNI CGCG 1 cut(s) 99
BstHHI GCGC 1 cut(s) 62
BstKTI GATC 1 cut(s) 478
BstMAI GTCTC 2 cut(s) 125, 218
BstMBI GATC 1 cut(s) 475
BstMWI GCNNNNNNNGC 1 cut(s) 72
BstNI CCWGG 1 cut(s) 156
BstNSI RCATGY 1 cut(s) 43
BstSCI CCNGG 2 cut(s) 154, 334
BstUI CGCG 1 cut(s) 99
BstV1I GCAGC 2 cut(s) 211, 354
BstV2I GAAGAC 1 cut(s) 279
BsuRI GGCC 1 cut(s) 5
Cac8I GCNNGC 2 cut(s) 64, 204
CfoI GCGC 1 cut(s) 62
Cfr13I GGNCC 1 cut(s) 265
CseI GACGC 3 cut(s) 88, 202, 348
CviAII CATG 4 cut(s) 7, 40, 269, 409
CviJI RGCY 8 cut(s) 5, 32, 52, 202, 345, 414, 430, 437
CviKI_1 RGCY 8 cut(s) 5, 32, 52, 202, 345, 414, 430, 437
DpnI GATC 1 cut(s) 477
DpnII GATC 1 cut(s) 475
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 255
Eco47I GGWCC 1 cut(s) 265
EcoRII CCWGG 1 cut(s) 154
Esp3I CGTCTC 1 cut(s) 125
FaeI CATG 4 cut(s) 10, 43, 272, 412
FaiI YATR 8 cut(s) 8, 41, 208, 244, 270, 311, 399, 410
FaqI GGGAC 1 cut(s) 291
FatI CATG 4 cut(s) 6, 39, 268, 408
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 2 cut(s) 200, 343
Fsp4HI GCNGC 2 cut(s) 200, 343
FspBI CTAG 2 cut(s) 12, 455
GlaI GCGC 1 cut(s) 61
GluI GCNGC 2 cut(s) 200, 343
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 336
HgaI GACGC 3 cut(s) 88, 202, 348
HhaI GCGC 1 cut(s) 62
Hin1II CATG 4 cut(s) 10, 43, 272, 412
Hin6I GCGC 1 cut(s) 60
HinP1I GCGC 1 cut(s) 60
HinfI GANTC 2 cut(s) 230, 287
HpaII CCGG 1 cut(s) 336
HphI GGTGA 2 cut(s) 178, 355
Hpy166II GTNNAC 1 cut(s) 103
Hpy188III TCNNGA 1 cut(s) 421
Hpy8I GTNNAC 1 cut(s) 103
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 1 cut(s) 91
HpyCH4III ACNGT 3 cut(s) 107, 320, 377
HpyCH4IV ACGT 3 cut(s) 68, 87, 354
HpyCH4V TGCA 6 cut(s) 39, 161, 180, 199, 206, 401
HpyF10VI GCNNNNNNNGC 1 cut(s) 72
HpySE526I ACGT 3 cut(s) 68, 87, 354
Hsp92II CATG 4 cut(s) 10, 43, 272, 412
HspAI GCGC 1 cut(s) 60
Kzo9I GATC 1 cut(s) 475
LpnPI CCDG 8 cut(s) 32, 141, 168, 172, 222, 349, 423, 467
Lsp1109I GCAGC 2 cut(s) 211, 354
LweI GCATC 1 cut(s) 110
MaeI CTAG 2 cut(s) 12, 455
MaeII ACGT 3 cut(s) 68, 87, 354
MaeIII GTNAC 4 cut(s) 107, 314, 361, 368
MalI GATC 1 cut(s) 477
MboI GATC 1 cut(s) 475
MboII GAAGA 3 cut(s) 272, 284, 409
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 145, 171, 279
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 296
MnlI CCTC 2 cut(s) 211, 256
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 384
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 336
MspR9I CCNGG 2 cut(s) 156, 336
MvaI CCWGG 1 cut(s) 156
MvnI CGCG 1 cut(s) 99
MwoI GCNNNNNNNGC 1 cut(s) 72
NciI CCSGG 1 cut(s) 336
NdeII GATC 1 cut(s) 475
NlaIII CATG 4 cut(s) 10, 43, 272, 412
NmuCI GTSAC 3 cut(s) 107, 314, 361
NspI RCATGY 1 cut(s) 43
PcsI WCGNNNNNNNCGW 1 cut(s) 140
PfeI GAWTC 1 cut(s) 230
PflMI CCANNNNNTGG 1 cut(s) 437
PkrI GCNGC 2 cut(s) 201, 344
PleI GAGTC 1 cut(s) 295
PpsI GAGTC 1 cut(s) 295
Psp1406I AACGTT 1 cut(s) 354
Psp6I CCWGG 1 cut(s) 154
PspGI CCWGG 1 cut(s) 154
PspPI GGNCC 1 cut(s) 265
SaqAI TTAA 1 cut(s) 384
SatI GCNGC 2 cut(s) 200, 343
Sau3AI GATC 1 cut(s) 475
Sau96I GGNCC 1 cut(s) 265
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 2 cut(s) 156, 336
SetI ASST 6 cut(s) 71, 90, 97, 197, 204, 357
SfaNI GCATC 1 cut(s) 110
SinI GGWCC 1 cut(s) 265
Sse9I AATT 3 cut(s) 145, 171, 279
SsiI CCGC 1 cut(s) 123
SspMI CTAG 2 cut(s) 12, 455
StyD4I CCNGG 2 cut(s) 154, 334
TaaI ACNGT 3 cut(s) 107, 320, 377
TaiI ACGT 3 cut(s) 71, 90, 357
TaqI TCGA 2 cut(s) 216, 233
TasI AATT 3 cut(s) 145, 171, 279
TfiI GAWTC 1 cut(s) 230
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TseFI GTSAC 3 cut(s) 107, 314, 361
TseI GCWGC 2 cut(s) 199, 342
Tsp45I GTSAC 3 cut(s) 107, 314, 361
TspDTI ATGAA 1 cut(s) 285
Van91I CCANNNNNTGG 1 cut(s) 437
VpaK11BI GGWCC 1 cut(s) 265
XapI RAATTY 1 cut(s) 279
XceI RCATGY 1 cut(s) 43
XcmI CCANNNNNNNNNTGG 1 cut(s) 13
XmiI GTMKAC 1 cut(s) 102
XspI CTAG 2 cut(s) 12, 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.