Rorug02G0194100

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001299
Physical Location & Seq
Reverse (-)
18060286 .. 18063698
3413 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0194100.1

Sequence Viewer

Length: 1242 bp
ATGGGATTTGCAATTTTCCTAACCTTCAAGCATCCAAACACTGGGAATTGGCGCATTAAACACTTTTTGGATCGTCACTCTATCCTAATGTTCTTGTTGGTTCTTCTCTTTACTCTGCTAGCCATAGTTGCATCAAGTTCGGCGCTAGTAGTAAATCACAGCTCTATGAAAGGAGCGGAGAAATTGAAGAGGACCATATTCAGCCTAGCTGAAAATGCTAGCCAAACCATCAGCAAAGTTACAAAAGCAATGAGAAAAATGGAATACATATTACTCCCTTATGATAAAGGCATGTCTATGAGCTTGAATAAGACATCCCATCAACTTGGAAAAGGATTGCGTTCGATTCAAACATTGGTTGACAACAATAGCCATACTCTTAACCAGGCAATTCAAGCCCCATATATCGCTCATCTTGTGATTGTAACGGTCAATTTGGTGGTACTGGTTGTGGCATTAGTTCTTCTCTTGCTGCATTGGTACCCAGGATTCGTAACTATAATCATTCTGTGCTGGATATTAACGACTCTGGTGTGGGTCCTGGCTGGTTTAGATTTCTTCCTTCACAATTTTGCAGATGATTCATGTTCAGCCCTAGAGGAATTCGAACGGAATGATAACAACAATACTCTAAGCTCAATCCTGCCATGCTTGGATCCGAAAAACTCAGATGCTCTGTTGACTGAAATTGGCTCTACTATATATAATTTCATAGATAAGTTGAATTCTAAAGTAGTAGAATTTTCTAGGTTGCTTGGAATGAATGAACAAAATACTGATTTTGCATTTCTACGTGTATGCAATCCTTTCTCCGGAGGACCTAAATTCAGCTACGAACCAGAACGTTGCCTAAATAGTCACATTACAATCGAAAAACTACCAGATGCCATTGCAGGAGTAACTTGTTACGATGACCAGGACAAGGAAGCATGCAAAAGAAGTGGAAGACTCCTTCCCCATACCTATTACAACATGGCCAGTGCTTACAGCCAATCCATCCAAGAATTTCTGAAACTATATCCTACTCTGCAGAGCCTAGCCTACTGCACATTTGTCAAGGATGGGATTTCCGAGGTCGTCAGGTATCAATGCAGGCCAATTAAGCTTGCGTTTCGACTGCTATGGGCGTCCATCCTCTCCCTTGCCATTTTCATGGTGTTTTTAGTAGTACTTCTGGTGGCAAGAGCTTTACAAGAGAAAGGTAGAGACTTCACTATATTTACCATCACCCTTTCCCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009505 GO:0016020 GO:0030312 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

413

Amino Acids

46.6

Weight (kDa)

8.62

Isoelectric Point (pI)

36.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 480
AccB1I GGYRCC 1 cut(s) 480
AccB7I CCANNNNNTGG 1 cut(s) 41
AccBSI CCGCTC 1 cut(s) 176
AccIII TCCGGA 1 cut(s) 812
AciI CCGC 1 cut(s) 176
AclI AACGTT 1 cut(s) 844
AclWI GGATC 3 cut(s) 78, 650, 663
AcoI YGGCCR 1 cut(s) 975
AcsI RAATTY 5 cut(s) 602, 724, 740, 824, 1004
AcyI GRCGYC 1 cut(s) 1127
AfaI GTAC 3 cut(s) 444, 482, 1170
AfiI CCNNNNNNNGG 3 cut(s) 41, 812, 922
AflIII ACRYGT 1 cut(s) 793
AgsI TTSAA 6 cut(s) 28, 187, 307, 350, 395, 724
AjnI CCWGG 4 cut(s) 384, 484, 540, 915
AluBI AGCT 7 cut(s) 162, 209, 303, 636, 831, 1105, 1187
AluI AGCT 7 cut(s) 162, 209, 303, 636, 831, 1105, 1187
Alw26I GTCTC 1 cut(s) 1200
AlwI GGATC 3 cut(s) 78, 650, 663
Aor13HI TCCGGA 1 cut(s) 812
AoxI GGCC 2 cut(s) 975, 1094
ApeKI GCWGC 1 cut(s) 472
ApoI RAATTY 5 cut(s) 602, 724, 740, 824, 1004
Asp718I GGTACC 1 cut(s) 480
AspLEI GCGC 2 cut(s) 54, 145
AspS9I GGNCC 3 cut(s) 192, 538, 818
AsuHPI GGTGA 1 cut(s) 1219
AsuII TTCGAA 1 cut(s) 606
AsuNHI GCTAGC 2 cut(s) 118, 218
AvaII GGWCC 3 cut(s) 192, 538, 818
BalI TGGCCA 1 cut(s) 977
BamHI GGATCC 1 cut(s) 655
BanI GGYRCC 1 cut(s) 480
BbsI GAAGAC 1 cut(s) 952
BbvI GCAGC 1 cut(s) 459
BccI CCATC 6 cut(s) 236, 327, 1004, 1055, 1139, 1232
BcgI CGANNNNNNTGC 2 cut(s) 120, 154
BciT130I CCWGG 4 cut(s) 386, 486, 542, 917
BcoDI GTCTC 1 cut(s) 1200
BfaI CTAG 7 cut(s) 119, 146, 206, 219, 596, 747, 1037
BfmI CTRYAG 1 cut(s) 1028
BfoI RGCGCY 1 cut(s) 146
BisI GCNGC 1 cut(s) 473
BlsI GCNGC 1 cut(s) 474
BmcAI AGTACT 1 cut(s) 1170
Bme1390I CCNGG 4 cut(s) 386, 486, 542, 917
Bme18I GGWCC 3 cut(s) 192, 538, 818
BmgT120I GGNCC 3 cut(s) 192, 538, 818
BmiI GGNNCC 3 cut(s) 482, 539, 657
BmrFI CCNGG 4 cut(s) 386, 486, 542, 917
BmrI ACTGGG 1 cut(s) 51
BmsI GCATC 4 cut(s) 40, 140, 661, 874
BmtI GCTAGC 2 cut(s) 122, 222
BmuI ACTGGG 1 cut(s) 51
BpiI GAAGAC 1 cut(s) 952
Bpu14I TTCGAA 1 cut(s) 606
BsaAI YACGTR 1 cut(s) 794
BsaHI GRCGYC 1 cut(s) 1127
BsaJI CCNNGG 2 cut(s) 484, 1071
BsaWI WCCGGW 1 cut(s) 812
Bsc4I CCNNNNNNNGG 3 cut(s) 41, 812, 922
Bse1I ACTGG 3 cut(s) 46, 450, 978
Bse3DI GCAATG 2 cut(s) 255, 888
BseAI TCCGGA 1 cut(s) 812
BseBI CCWGG 4 cut(s) 386, 486, 542, 917
BseDI CCNNGG 2 cut(s) 484, 1071
BseGI GGATG 5 cut(s) 31, 314, 996, 1066, 1131
BseLI CCNNNNNNNGG 3 cut(s) 41, 812, 922
BseMI GCAATG 2 cut(s) 255, 888
BseMII CTCAG 1 cut(s) 681
BseNI ACTGG 3 cut(s) 46, 450, 978
BseXI GCAGC 1 cut(s) 459
BsgI GTGCAG 1 cut(s) 1030
BshFI GGCC 2 cut(s) 977, 1096
BshNI GGYRCC 1 cut(s) 480
BsiSI CCGG 1 cut(s) 813
BslI CCNNNNNNNGG 3 cut(s) 41, 812, 922
BsmAI GTCTC 1 cut(s) 1200
BsnI GGCC 2 cut(s) 977, 1096
Bsp119I TTCGAA 1 cut(s) 606
Bsp13I TCCGGA 1 cut(s) 812
Bsp143I GATC 2 cut(s) 70, 655
BspACI CCGC 1 cut(s) 176
BspANI GGCC 2 cut(s) 977, 1096
BspCNI CTCAG 1 cut(s) 680
BspEI TCCGGA 1 cut(s) 812
BspLI GGNNCC 3 cut(s) 482, 539, 657
BspMAI CTGCAG 1 cut(s) 1032
BspOI GCTAGC 2 cut(s) 122, 222
BspPI GGATC 3 cut(s) 78, 650, 663
BspT104I TTCGAA 1 cut(s) 606
BspT107I GGYRCC 1 cut(s) 480
BsrBI CCGCTC 1 cut(s) 176
BsrDI GCAATG 2 cut(s) 255, 888
BsrI ACTGG 3 cut(s) 46, 450, 978
BssECI CCNNGG 2 cut(s) 484, 1071
BssMI GATC 2 cut(s) 70, 655
BssNI GRCGYC 1 cut(s) 1127
Bst2UI CCWGG 4 cut(s) 386, 486, 542, 917
Bst4CI ACNGT 1 cut(s) 430
Bst6I CTCTTC 1 cut(s) 182
BstACI GRCGYC 1 cut(s) 1127
BstBAI YACGTR 1 cut(s) 794
BstBI TTCGAA 1 cut(s) 606
BstC8I GCNNGC 5 cut(s) 120, 220, 931, 1094, 1107
BstDEI CTNAG 2 cut(s) 632, 667
BstF5I GGATG 5 cut(s) 31, 314, 996, 1066, 1131
BstH2I RGCGCY 1 cut(s) 146
BstHHI GCGC 2 cut(s) 54, 145
BstKTI GATC 2 cut(s) 73, 658
BstMAI GTCTC 1 cut(s) 1200
BstMBI GATC 2 cut(s) 70, 655
BstMWI GCNNNNNNNGC 4 cut(s) 128, 215, 395, 1102
BstNI CCWGG 4 cut(s) 386, 486, 542, 917
BstNSI RCATGY 2 cut(s) 295, 933
BstSCI CCNGG 4 cut(s) 384, 484, 540, 915
BstSFI CTRYAG 1 cut(s) 1028
BstV1I GCAGC 1 cut(s) 459
BstV2I GAAGAC 1 cut(s) 952
BstX2I RGATCY 1 cut(s) 655
BstXI CCANNNNNNTGG 2 cut(s) 326, 1153
BstYI RGATCY 1 cut(s) 655
BsuRI GGCC 2 cut(s) 977, 1096
BtsCI GGATG 5 cut(s) 31, 314, 996, 1066, 1131
BtsIMutI CAGTG 2 cut(s) 39, 985
Cac8I GCNNGC 5 cut(s) 120, 220, 931, 1094, 1107
CfoI GCGC 2 cut(s) 54, 145
Cfr13I GGNCC 3 cut(s) 192, 538, 818
CseI GACGC 1 cut(s) 1116
Csp6I GTAC 3 cut(s) 443, 481, 1169
CspCI CAANNNNNGTGG 2 cut(s) 922, 957
CviAII CATG 6 cut(s) 292, 585, 648, 930, 973, 1153
CviQI GTAC 3 cut(s) 443, 481, 1169
DdeI CTNAG 2 cut(s) 632, 667
DpnI GATC 2 cut(s) 72, 657
DpnII GATC 2 cut(s) 70, 655
EaeI YGGCCR 1 cut(s) 975
Eam1104I CTCTTC 1 cut(s) 182
EarI CTCTTC 1 cut(s) 182
Eco47I GGWCC 3 cut(s) 192, 538, 818
EcoO109I RGGNCCY 2 cut(s) 538, 818
EcoRI GAATTC 2 cut(s) 602, 724
EcoRII CCWGG 4 cut(s) 384, 484, 540, 915
FaeI CATG 6 cut(s) 295, 588, 651, 933, 976, 1156
FatI CATG 6 cut(s) 291, 584, 647, 929, 972, 1152
Fnu4HI GCNGC 1 cut(s) 473
FokI GGATG 5 cut(s) 18, 301, 983, 1073, 1118
Fsp4HI GCNGC 1 cut(s) 473
FspBI CTAG 7 cut(s) 119, 146, 206, 219, 596, 747, 1037
GlaI GCGC 2 cut(s) 53, 144
GluI GCNGC 1 cut(s) 473
HaeII RGCGCY 1 cut(s) 146
HaeIII GGCC 2 cut(s) 977, 1096
HapII CCGG 1 cut(s) 813
HgaI GACGC 1 cut(s) 1116
HhaI GCGC 2 cut(s) 54, 145
Hin1I GRCGYC 1 cut(s) 1127
Hin1II CATG 6 cut(s) 295, 588, 651, 933, 976, 1156
Hin6I GCGC 2 cut(s) 52, 143
HinP1I GCGC 2 cut(s) 52, 143
HincII GTYRAC 2 cut(s) 361, 681
HindII GTYRAC 2 cut(s) 361, 681
HindIII AAGCTT 1 cut(s) 1103
HinfI GANTC 5 cut(s) 346, 489, 526, 581, 948
HpaII CCGG 1 cut(s) 813
HphI GGTGA 1 cut(s) 1219
Hpy166II GTNNAC 2 cut(s) 361, 681
Hpy188I TCNGA 4 cut(s) 660, 670, 1011, 1072
Hpy188III TCNNGA 1 cut(s) 813
Hpy8I GTNNAC 2 cut(s) 361, 681
HpyAV CCTTC 3 cut(s) 34, 572, 962
HpyCH4III ACNGT 1 cut(s) 430
HpyCH4IV ACGT 2 cut(s) 793, 844
HpyF10VI GCNNNNNNNGC 4 cut(s) 128, 215, 395, 1102
HpyF3I CTNAG 2 cut(s) 632, 667
HpySE526I ACGT 2 cut(s) 793, 844
Hsp92I GRCGYC 1 cut(s) 1127
Hsp92II CATG 6 cut(s) 295, 588, 651, 933, 976, 1156
HspAI GCGC 2 cut(s) 52, 143
Kpn2I TCCGGA 1 cut(s) 812
KpnI GGTACC 1 cut(s) 484
Kzo9I GATC 2 cut(s) 70, 655
LmnI GCTCC 1 cut(s) 173
Lsp1109I GCAGC 1 cut(s) 459
LweI GCATC 4 cut(s) 40, 140, 661, 874
MaeI CTAG 7 cut(s) 119, 146, 206, 219, 596, 747, 1037
MaeII ACGT 2 cut(s) 793, 844
MaeIII GTNAC 7 cut(s) 74, 238, 424, 493, 857, 898, 905
MalI GATC 2 cut(s) 72, 657
MbiI CCGCTC 1 cut(s) 176
MboI GATC 2 cut(s) 70, 655
MboII GAAGA 5 cut(s) 95, 199, 455, 550, 957
MflI RGATCY 1 cut(s) 655
MlsI TGGCCA 1 cut(s) 977
MluNI TGGCCA 1 cut(s) 977
MlyI GAGTC 2 cut(s) 520, 942
MnlI CCTC 5 cut(s) 183, 592, 809, 1066, 1145
Mox20I TGGCCA 1 cut(s) 977
MroI TCCGGA 1 cut(s) 812
MscI TGGCCA 1 cut(s) 977
MseI TTAA 4 cut(s) 57, 381, 521, 1101
MslI CAYNNNNRTG 2 cut(s) 296, 1151
Msp20I TGGCCA 1 cut(s) 977
MspI CCGG 1 cut(s) 813
MspR9I CCNGG 4 cut(s) 386, 486, 542, 917
MvaI CCWGG 4 cut(s) 386, 486, 542, 917
MwoI GCNNNNNNNGC 4 cut(s) 128, 215, 395, 1102
NdeII GATC 2 cut(s) 70, 655
NheI GCTAGC 2 cut(s) 118, 218
NlaIII CATG 6 cut(s) 295, 588, 651, 933, 976, 1156
NlaIV GGNNCC 3 cut(s) 482, 539, 657
NmuCI GTSAC 2 cut(s) 74, 857
NspI RCATGY 2 cut(s) 295, 933
NspV TTCGAA 1 cut(s) 606
PaeI GCATGC 1 cut(s) 933
PfeI GAWTC 3 cut(s) 346, 489, 581
PflMI CCANNNNNTGG 1 cut(s) 41
PkrI GCNGC 1 cut(s) 474
PleI GAGTC 2 cut(s) 520, 942
PpsI GAGTC 2 cut(s) 520, 942
Ppu21I YACGTR 1 cut(s) 794
PpuMI RGGWCCY 2 cut(s) 538, 818
Psp1406I AACGTT 1 cut(s) 844
Psp5II RGGWCCY 2 cut(s) 538, 818
Psp6I CCWGG 4 cut(s) 384, 484, 540, 915
PspGI CCWGG 4 cut(s) 384, 484, 540, 915
PspN4I GGNNCC 3 cut(s) 482, 539, 657
PspPI GGNCC 3 cut(s) 192, 538, 818
PspPPI RGGWCCY 2 cut(s) 538, 818
PstI CTGCAG 1 cut(s) 1032
PsuI RGATCY 1 cut(s) 655
RsaI GTAC 3 cut(s) 444, 482, 1170
RsaNI GTAC 3 cut(s) 443, 481, 1169
RseI CAYNNNNRTG 2 cut(s) 296, 1151
SaqAI TTAA 4 cut(s) 57, 381, 521, 1101
SatI GCNGC 1 cut(s) 473
Sau3AI GATC 2 cut(s) 70, 655
Sau96I GGNCC 3 cut(s) 192, 538, 818
ScaI AGTACT 1 cut(s) 1170
SchI GAGTC 2 cut(s) 520, 942
ScrFI CCNGG 4 cut(s) 386, 486, 542, 917
SfaNI GCATC 4 cut(s) 40, 140, 661, 874
SfcI CTRYAG 1 cut(s) 1028
SfuI TTCGAA 1 cut(s) 606
SinI GGWCC 3 cut(s) 192, 538, 818
SmiMI CAYNNNNRTG 2 cut(s) 296, 1151
SphI GCATGC 1 cut(s) 933
SsiI CCGC 1 cut(s) 176
SspMI CTAG 7 cut(s) 119, 146, 206, 219, 596, 747, 1037
StyD4I CCNGG 4 cut(s) 384, 484, 540, 915
TaaI ACNGT 1 cut(s) 430
TaiI ACGT 2 cut(s) 796, 847
TaqI TCGA 4 cut(s) 344, 606, 870, 1114
TatI WGTACW 1 cut(s) 1168
TfiI GAWTC 3 cut(s) 346, 489, 581
Tru1I TTAA 4 cut(s) 57, 381, 521, 1101
Tru9I TTAA 4 cut(s) 57, 381, 521, 1101
TscAI CASTG 2 cut(s) 46, 985
TseFI GTSAC 2 cut(s) 74, 857
TseI GCWGC 1 cut(s) 472
Tsp45I GTSAC 2 cut(s) 74, 857
TspDTI ATGAA 6 cut(s) 182, 573, 700, 776, 780, 1141
TspGWI ACGGA 1 cut(s) 625
TspRI CASTG 2 cut(s) 46, 985
Van91I CCANNNNNTGG 1 cut(s) 41
VpaK11BI GGWCC 3 cut(s) 192, 538, 818
XapI RAATTY 5 cut(s) 602, 724, 740, 824, 1004
XceI RCATGY 2 cut(s) 295, 933
XspI CTAG 7 cut(s) 119, 146, 206, 219, 596, 747, 1037
ZrmI AGTACT 1 cut(s) 1170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.