MD13G1011500.v1.1

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001299
Physical Location & Seq
Reverse (-)
690577 .. 691220
644 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1011500.v1.1.491

Sequence Viewer

Length: 264 bp
ATGGAGCATTCTATGCGTCTTGTTTCAGCTGCCTTCGTTCTTGTCCTGCTTTTCGCTGCTACCGAGATGGGACCAATGGGTGTTGAGGCAAGGAGTAAGTCAGACAAGGTAGCTAAGGAAAGGACATGTGAGGCTGCGAGCGGCAAGTTCAAGGGGTTGTGCTTTTCGTCTACCAACTGTAAAAACACTTGCAAAGTGGAGAAATTCACCGGAGGCCAATGCCAGGGCTTTCGTCGTAGATGCATGTGCAACAAAAAGTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

88

Amino Acids

9.57

Weight (kDa)

9.43

Isoelectric Point (pI)

25.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 41 - 87 1.3e-15 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 141
AccI GTMKAC 1 cut(s) 170
AciI CCGC 1 cut(s) 141
AcsI RAATTY 1 cut(s) 203
AfiI CCNNNNNNNGG 1 cut(s) 223
AflIII ACRYGT 1 cut(s) 125
AgsI TTSAA 1 cut(s) 151
AjnI CCWGG 1 cut(s) 222
AluBI AGCT 2 cut(s) 29, 113
AluI AGCT 2 cut(s) 29, 113
AoxI GGCC 1 cut(s) 214
ApeKI GCWGC 3 cut(s) 29, 56, 134
ApoI RAATTY 1 cut(s) 203
AspS9I GGNCC 1 cut(s) 71
AsuHPI GGTGA 1 cut(s) 199
AvaII GGWCC 1 cut(s) 71
BbvI GCAGC 3 cut(s) 16, 43, 121
BccI CCATC 1 cut(s) 61
BciT130I CCWGG 1 cut(s) 224
BisI GCNGC 4 cut(s) 30, 57, 135, 142
BlsI GCNGC 4 cut(s) 31, 58, 136, 143
Bme1390I CCNGG 1 cut(s) 224
Bme18I GGWCC 1 cut(s) 71
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 1 cut(s) 224
BmsI GCATC 1 cut(s) 230
Bpu10I CCTNAGC 1 cut(s) 114
BsaJI CCNNGG 1 cut(s) 223
BsaWI WCCGGW 1 cut(s) 209
Bsc4I CCNNNNNNNGG 1 cut(s) 223
BseBI CCWGG 1 cut(s) 224
BseDI CCNNGG 1 cut(s) 223
BseLI CCNNNNNNNGG 1 cut(s) 223
BseXI GCAGC 3 cut(s) 16, 43, 121
BshFI GGCC 1 cut(s) 216
BsiSI CCGG 1 cut(s) 210
BslFI GGGAC 1 cut(s) 84
BslI CCNNNNNNNGG 1 cut(s) 223
BsmFI GGGAC 1 cut(s) 84
BsmI GAATGC 1 cut(s) 7
BsnI GGCC 1 cut(s) 216
BspACI CCGC 1 cut(s) 141
BspANI GGCC 1 cut(s) 216
BspLI GGNNCC 1 cut(s) 72
BsrBI CCGCTC 1 cut(s) 141
BssECI CCNNGG 1 cut(s) 223
Bst2UI CCWGG 1 cut(s) 224
Bst4CI ACNGT 1 cut(s) 179
BstAPI GCANNNNNTGC 1 cut(s) 13
BstC8I GCNNGC 1 cut(s) 139
BstDEI CTNAG 1 cut(s) 114
BstMWI GCNNNNNNNGC 1 cut(s) 13
BstNI CCWGG 1 cut(s) 224
BstNSI RCATGY 2 cut(s) 129, 247
BstSCI CCNGG 1 cut(s) 222
BstV1I GCAGC 3 cut(s) 16, 43, 121
BsuRI GGCC 1 cut(s) 216
Cac8I GCNNGC 1 cut(s) 139
Cfr13I GGNCC 1 cut(s) 71
CseI GACGC 1 cut(s) 5
CviAII CATG 2 cut(s) 126, 244
CviJI RGCY 5 cut(s) 29, 113, 134, 216, 228
CviKI_1 RGCY 5 cut(s) 29, 113, 134, 216, 228
DdeI CTNAG 1 cut(s) 114
Eco47I GGWCC 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 222
EcoT22I ATGCAT 1 cut(s) 245
FaeI CATG 2 cut(s) 129, 247
FaiI YATR 3 cut(s) 14, 127, 245
FaqI GGGAC 1 cut(s) 84
FatI CATG 2 cut(s) 125, 243
FblI GTMKAC 1 cut(s) 170
Fnu4HI GCNGC 4 cut(s) 30, 57, 135, 142
Fsp4HI GCNGC 4 cut(s) 30, 57, 135, 142
GluI GCNGC 4 cut(s) 30, 57, 135, 142
HaeIII GGCC 1 cut(s) 216
HapII CCGG 1 cut(s) 210
HgaI GACGC 1 cut(s) 5
Hin1II CATG 2 cut(s) 129, 247
HpaII CCGG 1 cut(s) 210
HphI GGTGA 1 cut(s) 199
Hpy166II GTNNAC 1 cut(s) 171
Hpy188I TCNGA 1 cut(s) 103
Hpy8I GTNNAC 1 cut(s) 171
Hpy99I CGWCG 1 cut(s) 237
HpyAV CCTTC 1 cut(s) 43
HpyCH4III ACNGT 1 cut(s) 179
HpyCH4V TGCA 3 cut(s) 192, 243, 249
HpyF10VI GCNNNNNNNGC 1 cut(s) 13
HpyF3I CTNAG 1 cut(s) 114
Hsp92II CATG 2 cut(s) 129, 247
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 4 cut(s) 59, 209, 223, 236
Lsp1109I GCAGC 3 cut(s) 16, 43, 121
LweI GCATC 1 cut(s) 230
MbiI CCGCTC 1 cut(s) 141
MluCI AATT 1 cut(s) 203
MnlI CCTC 3 cut(s) 79, 124, 206
Mph1103I ATGCAT 1 cut(s) 245
MspA1I CMGCKG 1 cut(s) 29
MspI CCGG 1 cut(s) 210
MspR9I CCNGG 1 cut(s) 224
Mva1269I GAATGC 1 cut(s) 7
MvaI CCWGG 1 cut(s) 224
MwoI GCNNNNNNNGC 1 cut(s) 13
NlaIII CATG 2 cut(s) 129, 247
NlaIV GGNNCC 1 cut(s) 72
NsiI ATGCAT 1 cut(s) 245
NspI RCATGY 2 cut(s) 129, 247
PciI ACATGT 1 cut(s) 125
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PctI GAATGC 1 cut(s) 7
PkrI GCNGC 4 cut(s) 31, 58, 136, 143
PscI ACATGT 1 cut(s) 125
Psp6I CCWGG 1 cut(s) 222
PspGI CCWGG 1 cut(s) 222
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 71
PvuII CAGCTG 1 cut(s) 29
SatI GCNGC 4 cut(s) 30, 57, 135, 142
Sau96I GGNCC 1 cut(s) 71
ScrFI CCNGG 1 cut(s) 224
SetI ASST 3 cut(s) 31, 111, 115
SfaNI GCATC 1 cut(s) 230
SinI GGWCC 1 cut(s) 71
Sse9I AATT 1 cut(s) 203
SsiI CCGC 1 cut(s) 141
StyD4I CCNGG 1 cut(s) 222
TaaI ACNGT 1 cut(s) 179
TasI AATT 1 cut(s) 203
TauI GCSGC 1 cut(s) 144
TseI GCWGC 3 cut(s) 29, 56, 134
VpaK11BI GGWCC 1 cut(s) 71
XapI RAATTY 1 cut(s) 203
XceI RCATGY 2 cut(s) 129, 247
XmiI GTMKAC 1 cut(s) 170
Zsp2I ATGCAT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.