MD14G1073000.v1.1

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001299
Physical Location & Seq
Forward (+)
8115484 .. 8117502
2019 bp
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UTR
Exon/CDS
Intron
MD14G1073000.v1.1.491

Sequence Viewer

Length: 264 bp
ATGGAGCATTCAATACGTCTTGTTTCAGCCGCCTTCGTTCTTGTCCTACTTTTGGCCGCTACAGAGATGGGGCCAATTGGTGTTGAGGCAAGGACTGAGTCTAGCAAGGCAATTGAGGGAAAGATCTGCGAGTTTCCAAGCACCTTGTTCAAGGGTTTGTGCTTTTCGTCTAACAACTGTAAACACACATGCAGAAAGGAGCAATTCACCAGAGGCAAATGTAGTCTCCTCACCCGTACATGCATGTGCACCAAAAAGTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

88

Amino Acids

9.59

Weight (kDa)

9.04

Isoelectric Point (pI)

30.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 41 - 87 1.4e-13 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 30, 57
AcoI YGGCCR 1 cut(s) 54
AfaI GTAC 1 cut(s) 238
AfiI CCNNNNNNNGG 1 cut(s) 52
AgsI TTSAA 2 cut(s) 12, 151
Alw21I GWGCWC 1 cut(s) 251
Alw26I GTCTC 1 cut(s) 230
Alw44I GTGCAC 1 cut(s) 247
AoxI GGCC 2 cut(s) 54, 71
ApaLI GTGCAC 1 cut(s) 247
AspS9I GGNCC 1 cut(s) 71
AsuHPI GGTGA 2 cut(s) 199, 223
BaeGI GKGCMC 1 cut(s) 251
Bbv12I GWGCWC 1 cut(s) 251
BccI CCATC 1 cut(s) 61
BcoDI GTCTC 1 cut(s) 230
BfaI CTAG 1 cut(s) 102
BfmI CTRYAG 1 cut(s) 60
BglII AGATCT 1 cut(s) 123
BisI GCNGC 2 cut(s) 30, 57
BlsI GCNGC 2 cut(s) 31, 58
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 1 cut(s) 72
Bsc4I CCNNNNNNNGG 1 cut(s) 52
BseLI CCNNNNNNNGG 1 cut(s) 52
BseMII CTCAG 1 cut(s) 87
BseRI GAGGAG 1 cut(s) 218
BseSI GKGCMC 1 cut(s) 251
BshFI GGCC 2 cut(s) 56, 73
BsiHKAI GWGCWC 1 cut(s) 251
BslI CCNNNNNNNGG 1 cut(s) 52
BsmAI GTCTC 1 cut(s) 230
BsmI GAATGC 1 cut(s) 7
BsnI GGCC 2 cut(s) 56, 73
Bsp1286I GDGCHC 1 cut(s) 251
Bsp143I GATC 1 cut(s) 123
BspACI CCGC 2 cut(s) 30, 57
BspANI GGCC 2 cut(s) 56, 73
BspCNI CTCAG 1 cut(s) 88
BspLI GGNNCC 1 cut(s) 72
BssMI GATC 1 cut(s) 123
Bst4CI ACNGT 1 cut(s) 179
BstDEI CTNAG 1 cut(s) 96
BstKTI GATC 1 cut(s) 126
BstMAI GTCTC 1 cut(s) 230
BstMBI GATC 1 cut(s) 123
BstNSI RCATGY 3 cut(s) 192, 243, 247
BstSFI CTRYAG 1 cut(s) 60
BstSLI GKGCMC 1 cut(s) 251
BstX2I RGATCY 1 cut(s) 123
BstYI RGATCY 1 cut(s) 123
BsuRI GGCC 2 cut(s) 56, 73
Cfr13I GGNCC 1 cut(s) 71
Csp6I GTAC 1 cut(s) 237
CviAII CATG 3 cut(s) 189, 240, 244
CviJI RGCY 3 cut(s) 29, 56, 73
CviKI_1 RGCY 3 cut(s) 29, 56, 73
CviQI GTAC 1 cut(s) 237
DdeI CTNAG 1 cut(s) 96
DpnI GATC 1 cut(s) 125
DpnII GATC 1 cut(s) 123
EaeI YGGCCR 1 cut(s) 54
EcoT22I ATGCAT 1 cut(s) 245
FaeI CATG 3 cut(s) 192, 243, 247
FaiI YATR 3 cut(s) 190, 241, 245
FatI CATG 3 cut(s) 188, 239, 243
Fnu4HI GCNGC 2 cut(s) 30, 57
Fsp4HI GCNGC 2 cut(s) 30, 57
FspBI CTAG 1 cut(s) 102
GluI GCNGC 2 cut(s) 30, 57
HaeIII GGCC 2 cut(s) 56, 73
Hin1II CATG 3 cut(s) 192, 243, 247
HinfI GANTC 1 cut(s) 98
HphI GGTGA 2 cut(s) 199, 223
Hpy166II GTNNAC 2 cut(s) 182, 249
Hpy8I GTNNAC 2 cut(s) 182, 249
HpyAV CCTTC 1 cut(s) 43
HpyCH4III ACNGT 1 cut(s) 179
HpyCH4IV ACGT 1 cut(s) 16
HpyCH4V TGCA 3 cut(s) 192, 243, 249
HpyF3I CTNAG 1 cut(s) 96
HpySE526I ACGT 1 cut(s) 16
Hsp92II CATG 3 cut(s) 192, 243, 247
Kzo9I GATC 1 cut(s) 123
LmnI GCTCC 2 cut(s) 4, 199
LpnPI CCDG 1 cut(s) 223
MaeI CTAG 1 cut(s) 102
MaeII ACGT 1 cut(s) 16
MalI GATC 1 cut(s) 125
MboI GATC 1 cut(s) 123
MfeI CAATTG 2 cut(s) 75, 111
MflI RGATCY 1 cut(s) 123
MhlI GDGCHC 1 cut(s) 251
MluCI AATT 3 cut(s) 75, 111, 203
MlyI GAGTC 1 cut(s) 107
MnlI CCTC 4 cut(s) 79, 109, 206, 239
Mph1103I ATGCAT 1 cut(s) 245
MslI CAYNNNNRTG 1 cut(s) 244
MunI CAATTG 2 cut(s) 75, 111
Mva1269I GAATGC 1 cut(s) 7
NdeII GATC 1 cut(s) 123
NlaIII CATG 3 cut(s) 192, 243, 247
NlaIV GGNNCC 1 cut(s) 72
NsiI ATGCAT 1 cut(s) 245
NspI RCATGY 3 cut(s) 192, 243, 247
PctI GAATGC 1 cut(s) 7
PflFI GACNNNGTC 1 cut(s) 97
PkrI GCNGC 2 cut(s) 31, 58
PleI GAGTC 1 cut(s) 106
PpsI GAGTC 1 cut(s) 106
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 71
PsuI RGATCY 1 cut(s) 123
PsyI GACNNNGTC 1 cut(s) 97
RsaI GTAC 1 cut(s) 238
RsaNI GTAC 1 cut(s) 237
RseI CAYNNNNRTG 1 cut(s) 244
SatI GCNGC 2 cut(s) 30, 57
Sau3AI GATC 1 cut(s) 123
Sau96I GGNCC 1 cut(s) 71
SchI GAGTC 1 cut(s) 107
SduI GDGCHC 1 cut(s) 251
SetI ASST 2 cut(s) 19, 146
SfcI CTRYAG 1 cut(s) 60
SmiMI CAYNNNNRTG 1 cut(s) 244
Sse9I AATT 3 cut(s) 75, 111, 203
SsiI CCGC 2 cut(s) 30, 57
SspMI CTAG 1 cut(s) 102
TaaI ACNGT 1 cut(s) 179
TaiI ACGT 1 cut(s) 19
TasI AATT 3 cut(s) 75, 111, 203
TauI GCSGC 2 cut(s) 32, 59
Tth111I GACNNNGTC 1 cut(s) 97
VneI GTGCAC 1 cut(s) 247
XceI RCATGY 3 cut(s) 192, 243, 247
XspI CTAG 1 cut(s) 102
Zsp2I ATGCAT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.