MD13G1011700.v1.1

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001299
Physical Location & Seq
Reverse (-)
699752 .. 700298
547 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1011700.v1.1.491

Sequence Viewer

Length: 264 bp
ATGGAGCGTTCAATGCGTCTGGTTTCAGCCGCCTTCATTCTTGTCATGTTTTTTGCTGCTACCGAGATGGGGCCAATGAGTGTTGAGGCAAGGACTGAGTCAAGCAAGGCAATTGAGGGAAAGATTTGCGAGGTTCCGAGCACCTTGTTCAAAGGGTTGTGCTTTTCGTCCAACAATTGTAAACACACCTGCAGAAAAGAGCAATTCACCAGAGGCAAATGTAGTGGCTTGACCCGTACATGCGTGTGCACCAAAAAGTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

88

Amino Acids

9.64

Weight (kDa)

9.2

Isoelectric Point (pI)

37.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 41 - 87 3.7e-14 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 197
Acc36I ACCTGC 1 cut(s) 197
AciI CCGC 1 cut(s) 30
AfaI GTAC 1 cut(s) 238
AfiI CCNNNNNNNGG 1 cut(s) 69
AgsI TTSAA 2 cut(s) 12, 151
Alw21I GWGCWC 2 cut(s) 143, 251
Alw44I GTGCAC 1 cut(s) 247
AoxI GGCC 1 cut(s) 71
ApaLI GTGCAC 1 cut(s) 247
ApeKI GCWGC 1 cut(s) 56
AspS9I GGNCC 1 cut(s) 71
AsuHPI GGTGA 1 cut(s) 199
BaeGI GKGCMC 1 cut(s) 251
Bbv12I GWGCWC 2 cut(s) 143, 251
BbvI GCAGC 1 cut(s) 43
BccI CCATC 1 cut(s) 61
BfmI CTRYAG 1 cut(s) 190
BfuAI ACCTGC 1 cut(s) 197
BisI GCNGC 2 cut(s) 30, 57
BlsI GCNGC 2 cut(s) 31, 58
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 2 cut(s) 72, 135
Bsc4I CCNNNNNNNGG 1 cut(s) 69
BseLI CCNNNNNNNGG 1 cut(s) 69
BseMII CTCAG 1 cut(s) 87
BseSI GKGCMC 1 cut(s) 251
BseXI GCAGC 1 cut(s) 43
BshFI GGCC 1 cut(s) 73
BsiHKAI GWGCWC 2 cut(s) 143, 251
BslI CCNNNNNNNGG 1 cut(s) 69
BsnI GGCC 1 cut(s) 73
Bsp1286I GDGCHC 2 cut(s) 143, 251
BspACI CCGC 1 cut(s) 30
BspANI GGCC 1 cut(s) 73
BspCNI CTCAG 1 cut(s) 88
BspLI GGNNCC 2 cut(s) 72, 135
BspMAI CTGCAG 1 cut(s) 194
BspMI ACCTGC 1 cut(s) 197
BstDEI CTNAG 1 cut(s) 96
BstMWI GCNNNNNNNGC 1 cut(s) 13
BstNSI RCATGY 1 cut(s) 243
BstSFI CTRYAG 1 cut(s) 190
BstSLI GKGCMC 1 cut(s) 251
BstV1I GCAGC 1 cut(s) 43
BsuRI GGCC 1 cut(s) 73
BveI ACCTGC 1 cut(s) 197
Cfr13I GGNCC 1 cut(s) 71
CseI GACGC 1 cut(s) 5
Csp6I GTAC 1 cut(s) 237
CspCI CAANNNNNGTGG 2 cut(s) 205, 240
CviAII CATG 2 cut(s) 46, 240
CviJI RGCY 3 cut(s) 29, 73, 228
CviKI_1 RGCY 3 cut(s) 29, 73, 228
CviQI GTAC 1 cut(s) 237
DdeI CTNAG 1 cut(s) 96
FaeI CATG 2 cut(s) 49, 243
FaiI YATR 2 cut(s) 47, 241
FatI CATG 2 cut(s) 45, 239
Fnu4HI GCNGC 2 cut(s) 30, 57
Fsp4HI GCNGC 2 cut(s) 30, 57
GluI GCNGC 2 cut(s) 30, 57
HaeIII GGCC 1 cut(s) 73
HgaI GACGC 1 cut(s) 5
Hin1II CATG 2 cut(s) 49, 243
HinfI GANTC 1 cut(s) 98
HphI GGTGA 1 cut(s) 199
Hpy166II GTNNAC 2 cut(s) 182, 249
Hpy188I TCNGA 1 cut(s) 138
Hpy8I GTNNAC 2 cut(s) 182, 249
HpyAV CCTTC 1 cut(s) 43
HpyCH4V TGCA 2 cut(s) 192, 249
HpyF10VI GCNNNNNNNGC 1 cut(s) 13
HpyF3I CTNAG 1 cut(s) 96
Hsp92II CATG 2 cut(s) 49, 243
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 3 cut(s) 5, 202, 223
Lsp1109I GCAGC 1 cut(s) 43
MfeI CAATTG 2 cut(s) 111, 175
MhlI GDGCHC 2 cut(s) 143, 251
MluCI AATT 3 cut(s) 111, 175, 203
MlyI GAGTC 1 cut(s) 107
MmeI TCCRAC 1 cut(s) 195
MnlI CCTC 4 cut(s) 79, 109, 124, 206
MslI CAYNNNNRTG 1 cut(s) 244
MunI CAATTG 2 cut(s) 111, 175
MwoI GCNNNNNNNGC 1 cut(s) 13
NlaIII CATG 2 cut(s) 49, 243
NlaIV GGNNCC 2 cut(s) 72, 135
NspI RCATGY 1 cut(s) 243
PaqCI CACCTGC 1 cut(s) 197
PflFI GACNNNGTC 1 cut(s) 97
PkrI GCNGC 2 cut(s) 31, 58
PleI GAGTC 1 cut(s) 106
PpsI GAGTC 1 cut(s) 106
PspN4I GGNNCC 2 cut(s) 72, 135
PspPI GGNCC 1 cut(s) 71
PstI CTGCAG 1 cut(s) 194
PsyI GACNNNGTC 1 cut(s) 97
RsaI GTAC 1 cut(s) 238
RsaNI GTAC 1 cut(s) 237
RseI CAYNNNNRTG 1 cut(s) 244
SatI GCNGC 2 cut(s) 30, 57
Sau96I GGNCC 1 cut(s) 71
SchI GAGTC 1 cut(s) 107
SduI GDGCHC 2 cut(s) 143, 251
SetI ASST 3 cut(s) 135, 146, 191
SfcI CTRYAG 1 cut(s) 190
SmiMI CAYNNNNRTG 1 cut(s) 244
Sse9I AATT 3 cut(s) 111, 175, 203
SsiI CCGC 1 cut(s) 30
TasI AATT 3 cut(s) 111, 175, 203
TauI GCSGC 1 cut(s) 32
TseI GCWGC 1 cut(s) 56
TspDTI ATGAA 1 cut(s) 25
Tth111I GACNNNGTC 1 cut(s) 97
VneI GTGCAC 1 cut(s) 247
XceI RCATGY 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.