pycom12g12050

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001299
Physical Location & Seq
Reverse (-)
14528126 .. 14528476
351 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g12050.1

Sequence Viewer

Length: 270 bp
ATGGAGTGTTCAATGCGTCTTGTTTCAGCTGCCTTCATTCTTGTCCTGCTTTTGGCCGCTACCGAGATGGGGCCAATGGGTATTGAGGCAAAGACTGAGTCGATCAAGGCAGTTGAGGGAAAGATCTGCGAGGTTCCGAGCACCCTGTTCAAGGGGTTGTGCGTTTCATCTAACAATTGTAAACACACCTGCAGAAAGGAGCAATTCACCAGAGGCAAATGTATTGGCGATGGCCTCACTCGTAAATGCGTGTGCACCAAAAAGTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

90

Amino Acids

9.65

Weight (kDa)

8.94

Isoelectric Point (pI)

26.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 41 - 89 1.1e-13 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 197
Acc36I ACCTGC 1 cut(s) 197
AciI CCGC 1 cut(s) 57
AcoI YGGCCR 1 cut(s) 54
AfiI CCNNNNNNNGG 3 cut(s) 52, 69, 151
AgsI TTSAA 2 cut(s) 12, 151
AluBI AGCT 1 cut(s) 29
AluI AGCT 1 cut(s) 29
Alw21I GWGCWC 2 cut(s) 143, 257
Alw44I GTGCAC 1 cut(s) 253
AoxI GGCC 3 cut(s) 54, 71, 232
ApaLI GTGCAC 1 cut(s) 253
ApeKI GCWGC 1 cut(s) 29
ArsI GACNNNNNNTTYG 2 cut(s) 83, 115
AspS9I GGNCC 1 cut(s) 71
AsuHPI GGTGA 1 cut(s) 199
BaeGI GKGCMC 1 cut(s) 257
Bbv12I GWGCWC 2 cut(s) 143, 257
BbvI GCAGC 1 cut(s) 16
BccI CCATC 2 cut(s) 61, 224
BfmI CTRYAG 1 cut(s) 190
BfuAI ACCTGC 1 cut(s) 197
BglII AGATCT 1 cut(s) 123
BisI GCNGC 2 cut(s) 30, 57
BlsI GCNGC 2 cut(s) 31, 58
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 2 cut(s) 72, 135
Bsc4I CCNNNNNNNGG 3 cut(s) 52, 69, 151
BseLI CCNNNNNNNGG 3 cut(s) 52, 69, 151
BseMII CTCAG 1 cut(s) 87
BseSI GKGCMC 1 cut(s) 257
BseXI GCAGC 1 cut(s) 16
BshFI GGCC 3 cut(s) 56, 73, 234
BsiHKAI GWGCWC 2 cut(s) 143, 257
BslI CCNNNNNNNGG 3 cut(s) 52, 69, 151
BsnI GGCC 3 cut(s) 56, 73, 234
Bsp1286I GDGCHC 2 cut(s) 143, 257
Bsp143I GATC 2 cut(s) 102, 123
BspACI CCGC 1 cut(s) 57
BspANI GGCC 3 cut(s) 56, 73, 234
BspCNI CTCAG 1 cut(s) 88
BspLI GGNNCC 2 cut(s) 72, 135
BspMAI CTGCAG 1 cut(s) 194
BspMI ACCTGC 1 cut(s) 197
BssMI GATC 2 cut(s) 102, 123
BstDEI CTNAG 1 cut(s) 96
BstENI CCTNNNNNAGG 1 cut(s) 149
BstKTI GATC 2 cut(s) 105, 126
BstMBI GATC 2 cut(s) 102, 123
BstSFI CTRYAG 1 cut(s) 190
BstSLI GKGCMC 1 cut(s) 257
BstV1I GCAGC 1 cut(s) 16
BstX2I RGATCY 1 cut(s) 123
BstYI RGATCY 1 cut(s) 123
BsuRI GGCC 3 cut(s) 56, 73, 234
BtgZI GCGATG 1 cut(s) 243
BveI ACCTGC 1 cut(s) 197
Cfr13I GGNCC 1 cut(s) 71
CseI GACGC 1 cut(s) 5
CviJI RGCY 4 cut(s) 29, 56, 73, 234
CviKI_1 RGCY 4 cut(s) 29, 56, 73, 234
DdeI CTNAG 1 cut(s) 96
DpnI GATC 2 cut(s) 104, 125
DpnII GATC 2 cut(s) 102, 123
EaeI YGGCCR 1 cut(s) 54
EcoNI CCTNNNNNAGG 1 cut(s) 149
Fnu4HI GCNGC 2 cut(s) 30, 57
Fsp4HI GCNGC 2 cut(s) 30, 57
GluI GCNGC 2 cut(s) 30, 57
HaeIII GGCC 3 cut(s) 56, 73, 234
HgaI GACGC 1 cut(s) 5
HinfI GANTC 1 cut(s) 98
HphI GGTGA 1 cut(s) 199
Hpy166II GTNNAC 2 cut(s) 182, 255
Hpy188I TCNGA 1 cut(s) 138
Hpy8I GTNNAC 2 cut(s) 182, 255
HpyAV CCTTC 1 cut(s) 43
HpyCH4V TGCA 2 cut(s) 192, 255
HpyF3I CTNAG 1 cut(s) 96
Kzo9I GATC 2 cut(s) 102, 123
LmnI GCTCC 1 cut(s) 199
LpnPI CCDG 4 cut(s) 59, 158, 202, 223
Lsp1109I GCAGC 1 cut(s) 16
MalI GATC 2 cut(s) 104, 125
MboI GATC 2 cut(s) 102, 123
MfeI CAATTG 1 cut(s) 175
MflI RGATCY 1 cut(s) 123
MhlI GDGCHC 2 cut(s) 143, 257
MluCI AATT 2 cut(s) 175, 203
MlyI GAGTC 1 cut(s) 107
MnlI CCTC 5 cut(s) 79, 109, 124, 206, 245
MspA1I CMGCKG 1 cut(s) 29
MunI CAATTG 1 cut(s) 175
NdeII GATC 2 cut(s) 102, 123
NlaIV GGNNCC 2 cut(s) 72, 135
PaqCI CACCTGC 1 cut(s) 197
PflFI GACNNNGTC 1 cut(s) 97
PkrI GCNGC 2 cut(s) 31, 58
PleI GAGTC 1 cut(s) 106
PpsI GAGTC 1 cut(s) 106
PspN4I GGNNCC 2 cut(s) 72, 135
PspPI GGNCC 1 cut(s) 71
PstI CTGCAG 1 cut(s) 194
PsuI RGATCY 1 cut(s) 123
PsyI GACNNNGTC 1 cut(s) 97
PvuII CAGCTG 1 cut(s) 29
SatI GCNGC 2 cut(s) 30, 57
Sau3AI GATC 2 cut(s) 102, 123
Sau96I GGNCC 1 cut(s) 71
SchI GAGTC 1 cut(s) 107
SduI GDGCHC 2 cut(s) 143, 257
SetI ASST 3 cut(s) 31, 135, 191
SfcI CTRYAG 1 cut(s) 190
Sse9I AATT 2 cut(s) 175, 203
SsiI CCGC 1 cut(s) 57
TaqI TCGA 1 cut(s) 101
TasI AATT 2 cut(s) 175, 203
TauI GCSGC 1 cut(s) 59
TseI GCWGC 1 cut(s) 29
TspDTI ATGAA 2 cut(s) 25, 156
Tth111I GACNNNGTC 1 cut(s) 97
VneI GTGCAC 1 cut(s) 253
XagI CCTNNNNNAGG 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.