MD12G1061700.v1.1

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001299
Physical Location & Seq
Reverse (-)
7206494 .. 7207198
705 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1061700.v1.1.491

Sequence Viewer

Length: 273 bp
ATGGAGCGTTCCGGGCGTTTGTTTTCAACTGTCTTCATTCTCATCTTGCTTTTGGTGGCTATTGGAACGGGGCCAATGGTTGCTGAGGGAAAGACAAAAGAGGAGTCGTCAAAGAATGAGAAGTCATCCAAGATTTGTGAGTCTCCGAGCCATAAATACCGAGGTATTTGCTTTCGGTCAAGCAATTGTGCAACTACATGCAAAAAAGAGGGATTTATGGGAGGCAAATGTGCTGGTTTCCGGATGAGATGTGTTTGCACTAAAAAATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

91

Amino Acids

9.88

Weight (kDa)

9.5

Isoelectric Point (pI)

41.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 44 - 90 6.2e-16 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 240
AgsI TTSAA 1 cut(s) 27
Alw26I GTCTC 1 cut(s) 147
Aor13HI TCCGGA 1 cut(s) 240
AoxI GGCC 1 cut(s) 71
ArsI GACNNNNNNTTYG 2 cut(s) 89, 121
AspS9I GGNCC 1 cut(s) 71
AsuC2I CCSGG 1 cut(s) 13
BbsI GAAGAC 1 cut(s) 25
BbvCI CCTCAGC 1 cut(s) 84
BcgI CGANNNNNNTGC 2 cut(s) 150, 184
BcnI CCSGG 1 cut(s) 13
BcoDI GTCTC 1 cut(s) 147
Bme1390I CCNGG 1 cut(s) 13
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 1 cut(s) 13
BpiI GAAGAC 1 cut(s) 25
Bpu10I CCTNAGC 1 cut(s) 84
BpuMI CCSGG 1 cut(s) 13
BsaJI CCNNGG 1 cut(s) 160
BsaWI WCCGGW 1 cut(s) 240
BseAI TCCGGA 1 cut(s) 240
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 2 cut(s) 125, 249
BseMII CTCAG 1 cut(s) 75
BseRI GAGGAG 1 cut(s) 116
BshFI GGCC 1 cut(s) 73
BsiSI CCGG 2 cut(s) 12, 241
BsmAI GTCTC 1 cut(s) 147
BsnI GGCC 1 cut(s) 73
Bsp13I TCCGGA 1 cut(s) 240
BspANI GGCC 1 cut(s) 73
BspCNI CTCAG 1 cut(s) 76
BspEI TCCGGA 1 cut(s) 240
BspLI GGNNCC 1 cut(s) 72
BssECI CCNNGG 1 cut(s) 160
Bst4CI ACNGT 1 cut(s) 31
BstDEI CTNAG 1 cut(s) 84
BstF5I GGATG 2 cut(s) 125, 249
BstMAI GTCTC 1 cut(s) 147
BstMWI GCNNNNNNNGC 1 cut(s) 13
BstNSI RCATGY 1 cut(s) 201
BstSCI CCNGG 1 cut(s) 11
BstV2I GAAGAC 1 cut(s) 25
BsuRI GGCC 1 cut(s) 73
BtsCI GGATG 2 cut(s) 125, 249
Cfr13I GGNCC 1 cut(s) 71
CviAII CATG 1 cut(s) 198
CviJI RGCY 3 cut(s) 59, 73, 150
CviKI_1 RGCY 3 cut(s) 59, 73, 150
DdeI CTNAG 1 cut(s) 84
FaeI CATG 1 cut(s) 201
FaiI YATR 3 cut(s) 153, 199, 218
FatI CATG 1 cut(s) 197
FokI GGATG 2 cut(s) 112, 256
HaeIII GGCC 1 cut(s) 73
HapII CCGG 2 cut(s) 12, 241
Hin1II CATG 1 cut(s) 201
HinfI GANTC 2 cut(s) 104, 140
HpaII CCGG 2 cut(s) 12, 241
Hpy188I TCNGA 1 cut(s) 147
Hpy188III TCNNGA 1 cut(s) 241
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4V TGCA 3 cut(s) 191, 201, 258
HpyF10VI GCNNNNNNNGC 1 cut(s) 13
HpyF3I CTNAG 1 cut(s) 84
Hsp92II CATG 1 cut(s) 201
Kpn2I TCCGGA 1 cut(s) 240
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 3 cut(s) 25, 219, 254
MboII GAAGA 1 cut(s) 25
MfeI CAATTG 1 cut(s) 184
MluCI AATT 1 cut(s) 184
MlyI GAGTC 2 cut(s) 113, 149
MnlI CCTC 5 cut(s) 79, 94, 155, 202, 215
MroI TCCGGA 1 cut(s) 240
MseI TTAA 1 cut(s) 271
MspI CCGG 2 cut(s) 12, 241
MspR9I CCNGG 1 cut(s) 13
MunI CAATTG 1 cut(s) 184
MwoI GCNNNNNNNGC 1 cut(s) 13
NciI CCSGG 1 cut(s) 13
NlaIII CATG 1 cut(s) 201
NlaIV GGNNCC 1 cut(s) 72
NspI RCATGY 1 cut(s) 201
PleI GAGTC 2 cut(s) 112, 148
PpsI GAGTC 2 cut(s) 112, 148
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 71
SaqAI TTAA 1 cut(s) 271
Sau96I GGNCC 1 cut(s) 71
SchI GAGTC 2 cut(s) 113, 149
ScrFI CCNGG 1 cut(s) 13
SetI ASST 1 cut(s) 166
Sse9I AATT 1 cut(s) 184
StyD4I CCNGG 1 cut(s) 11
TaaI ACNGT 1 cut(s) 31
TaqII GACCGA 1 cut(s) 165
TasI AATT 1 cut(s) 184
Tru1I TTAA 1 cut(s) 271
Tru9I TTAA 1 cut(s) 271
TspDTI ATGAA 1 cut(s) 25
XceI RCATGY 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.