MD12G1121400.v1.1

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001299
Physical Location & Seq
Reverse (-)
19487516 .. 19488069
554 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1121400.v1.1.491

Sequence Viewer

Length: 270 bp
ATGGAGCGTTCAATGCGTCTTGTTTCAGCCGCCTTCGTTCTTGTCCTGCTTTTGGCGTCTACCGATATGGGGCCAATGGGCATTGAGGCAAAGACTGAATCAAGCAAGGCAGTTGAGGGAAAGATCTGCGAGGTTCCGAGCACCCTGTTCAAGGGGTTGTGCATTTCGTCTAACAACTGTAAACACACCTGCAGAAAGGAGCAATTCACCAGAGGCAAATGTATTGGCGATGGCTTCACTCGTAAATGCGTCTGCACCAAAAAGTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

90

Amino Acids

9.71

Weight (kDa)

9.16

Isoelectric Point (pI)

26.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 41 - 89 8.1e-14 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 197
Acc36I ACCTGC 1 cut(s) 197
AccI GTMKAC 1 cut(s) 59
AciI CCGC 1 cut(s) 30
AcyI GRCGYC 1 cut(s) 56
AfiI CCNNNNNNNGG 3 cut(s) 52, 69, 151
AgsI TTSAA 2 cut(s) 12, 151
Alw21I GWGCWC 1 cut(s) 143
AoxI GGCC 1 cut(s) 71
AspS9I GGNCC 1 cut(s) 71
AsuHPI GGTGA 1 cut(s) 199
Bbv12I GWGCWC 1 cut(s) 143
BccI CCATC 1 cut(s) 224
BfmI CTRYAG 1 cut(s) 190
BfuAI ACCTGC 1 cut(s) 197
BglII AGATCT 1 cut(s) 123
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 2 cut(s) 72, 135
BsaHI GRCGYC 1 cut(s) 56
Bsc4I CCNNNNNNNGG 3 cut(s) 52, 69, 151
BseLI CCNNNNNNNGG 3 cut(s) 52, 69, 151
BsgI GTGCAG 1 cut(s) 238
BshFI GGCC 1 cut(s) 73
BsiHKAI GWGCWC 1 cut(s) 143
BslI CCNNNNNNNGG 3 cut(s) 52, 69, 151
BsnI GGCC 1 cut(s) 73
Bsp1286I GDGCHC 1 cut(s) 143
Bsp143I GATC 1 cut(s) 123
BspACI CCGC 1 cut(s) 30
BspANI GGCC 1 cut(s) 73
BspLI GGNNCC 2 cut(s) 72, 135
BspMAI CTGCAG 1 cut(s) 194
BspMI ACCTGC 1 cut(s) 197
BssMI GATC 1 cut(s) 123
BssNI GRCGYC 1 cut(s) 56
Bst4CI ACNGT 1 cut(s) 179
BstACI GRCGYC 1 cut(s) 56
BstENI CCTNNNNNAGG 1 cut(s) 149
BstKTI GATC 1 cut(s) 126
BstMBI GATC 1 cut(s) 123
BstMWI GCNNNNNNNGC 1 cut(s) 13
BstSFI CTRYAG 1 cut(s) 190
BstX2I RGATCY 1 cut(s) 123
BstYI RGATCY 1 cut(s) 123
BsuRI GGCC 1 cut(s) 73
BtgZI GCGATG 1 cut(s) 243
BveI ACCTGC 1 cut(s) 197
Cfr13I GGNCC 1 cut(s) 71
CseI GACGC 3 cut(s) 5, 45, 238
CviJI RGCY 3 cut(s) 29, 73, 234
CviKI_1 RGCY 3 cut(s) 29, 73, 234
DpnI GATC 1 cut(s) 125
DpnII GATC 1 cut(s) 123
EcoNI CCTNNNNNAGG 1 cut(s) 149
FaiI YATR 1 cut(s) 68
FblI GTMKAC 1 cut(s) 59
Fnu4HI GCNGC 1 cut(s) 30
Fsp4HI GCNGC 1 cut(s) 30
GluI GCNGC 1 cut(s) 30
HaeIII GGCC 1 cut(s) 73
HgaI GACGC 3 cut(s) 5, 45, 238
Hin1I GRCGYC 1 cut(s) 56
HinfI GANTC 1 cut(s) 98
HphI GGTGA 1 cut(s) 199
Hpy166II GTNNAC 2 cut(s) 60, 182
Hpy188I TCNGA 1 cut(s) 138
Hpy8I GTNNAC 2 cut(s) 60, 182
HpyAV CCTTC 1 cut(s) 43
HpyCH4III ACNGT 1 cut(s) 179
HpyCH4V TGCA 3 cut(s) 162, 192, 255
HpyF10VI GCNNNNNNNGC 1 cut(s) 13
Hsp92I GRCGYC 1 cut(s) 56
Kzo9I GATC 1 cut(s) 123
LmnI GCTCC 2 cut(s) 4, 199
LpnPI CCDG 4 cut(s) 59, 158, 202, 223
MalI GATC 1 cut(s) 125
MboI GATC 1 cut(s) 123
MflI RGATCY 1 cut(s) 123
MhlI GDGCHC 1 cut(s) 143
MluCI AATT 1 cut(s) 203
MnlI CCTC 4 cut(s) 79, 109, 124, 206
MwoI GCNNNNNNNGC 1 cut(s) 13
NdeII GATC 1 cut(s) 123
NlaIV GGNNCC 2 cut(s) 72, 135
PaqCI CACCTGC 1 cut(s) 197
PfeI GAWTC 1 cut(s) 98
PkrI GCNGC 1 cut(s) 31
PspN4I GGNNCC 2 cut(s) 72, 135
PspPI GGNCC 1 cut(s) 71
PstI CTGCAG 1 cut(s) 194
PsuI RGATCY 1 cut(s) 123
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 1 cut(s) 123
Sau96I GGNCC 1 cut(s) 71
SduI GDGCHC 1 cut(s) 143
SetI ASST 2 cut(s) 135, 191
SfcI CTRYAG 1 cut(s) 190
Sse9I AATT 1 cut(s) 203
SsiI CCGC 1 cut(s) 30
TaaI ACNGT 1 cut(s) 179
TasI AATT 1 cut(s) 203
TauI GCSGC 1 cut(s) 32
TfiI GAWTC 1 cut(s) 98
XagI CCTNNNNNAGG 1 cut(s) 149
XmiI GTMKAC 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.