FvH4_1g21320

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001299
Physical Location & Seq
Reverse (-)
13297389 .. 13298219
831 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g21320.t1

Sequence Viewer

Length: 252 bp
ATGAACATGGATCGTTCACTATGTCTATCTGCCTTCCTTCTTGTCCTGGTTCTCATGGCTACAGAGATGGGGCCAGAAATGGTTGCCGGAGGAAGGCCGGATCCGAAGACATGCGAGTCTCCGAGCGCCAAGTTTGAGGGGCCTTGCATTATGGGAAGCAACTGCGCACTTACTTGCCAAAAAGAAGGCCACGACGGCGGCTTTTGTCGCGACTTCTGGCTGCCAAAATGCTTCTGCACTAAACCTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009505 GO:0016020 GO:0030312 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

84

Amino Acids

9.01

Weight (kDa)

5.68

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 36 - 83 1.3e-13 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 115
Acc16I TGCGCA 1 cut(s) 166
AccII CGCG 1 cut(s) 210
AciI CCGC 1 cut(s) 198
AclWI GGATC 3 cut(s) 18, 95, 108
AfiI CCNNNNNNNGG 1 cut(s) 93
AjnI CCWGG 1 cut(s) 45
Alw26I GTCTC 1 cut(s) 123
AlwI GGATC 3 cut(s) 18, 95, 108
AoxI GGCC 4 cut(s) 71, 95, 140, 187
ApeKI GCWGC 1 cut(s) 220
AspLEI GCGC 2 cut(s) 128, 167
AspS9I GGNCC 2 cut(s) 71, 140
BamHI GGATCC 1 cut(s) 100
BbsI GAAGAC 1 cut(s) 113
BbvI GCAGC 1 cut(s) 207
BccI CCATC 1 cut(s) 61
BceAI ACGGC 1 cut(s) 211
BciT130I CCWGG 1 cut(s) 47
BcoDI GTCTC 1 cut(s) 123
BfmI CTRYAG 1 cut(s) 60
BfoI RGCGCY 1 cut(s) 129
BglI GCCNNNNNGGC 1 cut(s) 195
BisI GCNGC 2 cut(s) 199, 221
BlsI GCNGC 2 cut(s) 200, 222
Bme1390I CCNGG 1 cut(s) 47
BmgT120I GGNCC 2 cut(s) 71, 140
BmiI GGNNCC 3 cut(s) 72, 102, 141
BmrFI CCNGG 1 cut(s) 47
BpiI GAAGAC 1 cut(s) 113
Bsc4I CCNNNNNNNGG 1 cut(s) 93
BseBI CCWGG 1 cut(s) 47
BseLI CCNNNNNNNGG 1 cut(s) 93
BseXI GCAGC 1 cut(s) 207
BsgI GTGCAG 1 cut(s) 220
Bsh1236I CGCG 1 cut(s) 210
BshFI GGCC 4 cut(s) 73, 97, 142, 189
BsiSI CCGG 2 cut(s) 87, 98
BslI CCNNNNNNNGG 1 cut(s) 93
BsmAI GTCTC 1 cut(s) 123
BsnI GGCC 4 cut(s) 73, 97, 142, 189
Bsp143I GATC 2 cut(s) 10, 100
Bsp68I TCGCGA 1 cut(s) 210
BspACI CCGC 1 cut(s) 198
BspANI GGCC 4 cut(s) 73, 97, 142, 189
BspFNI CGCG 1 cut(s) 210
BspLI GGNNCC 3 cut(s) 72, 102, 141
BspPI GGATC 3 cut(s) 18, 95, 108
BssMI GATC 2 cut(s) 10, 100
Bst2UI CCWGG 1 cut(s) 47
BstFNI CGCG 1 cut(s) 210
BstH2I RGCGCY 1 cut(s) 129
BstHHI GCGC 2 cut(s) 128, 167
BstKTI GATC 2 cut(s) 13, 103
BstMAI GTCTC 1 cut(s) 123
BstMBI GATC 2 cut(s) 10, 100
BstMWI GCNNNNNNNGC 2 cut(s) 195, 207
BstNI CCWGG 1 cut(s) 47
BstNSI RCATGY 1 cut(s) 114
BstSCI CCNGG 1 cut(s) 45
BstSFI CTRYAG 1 cut(s) 60
BstUI CGCG 1 cut(s) 210
BstV1I GCAGC 1 cut(s) 207
BstV2I GAAGAC 1 cut(s) 113
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BsuRI GGCC 4 cut(s) 73, 97, 142, 189
BtuMI TCGCGA 1 cut(s) 210
CfoI GCGC 2 cut(s) 128, 167
Cfr13I GGNCC 2 cut(s) 71, 140
CviAII CATG 3 cut(s) 7, 55, 111
CviJI RGCY 7 cut(s) 59, 73, 97, 142, 189, 201, 220
CviKI_1 RGCY 7 cut(s) 59, 73, 97, 142, 189, 201, 220
DpnI GATC 2 cut(s) 12, 102
DpnII GATC 2 cut(s) 10, 100
DrdI GACNNNNNNGTC 1 cut(s) 115
DseDI GACNNNNNNGTC 1 cut(s) 115
EcoO109I RGGNCCY 1 cut(s) 140
EcoRII CCWGG 1 cut(s) 45
FaeI CATG 3 cut(s) 10, 58, 114
FaiI YATR 5 cut(s) 8, 22, 56, 112, 152
FatI CATG 3 cut(s) 6, 54, 110
Fnu4HI GCNGC 2 cut(s) 199, 221
Fsp4HI GCNGC 2 cut(s) 199, 221
FspI TGCGCA 1 cut(s) 166
GlaI GCGC 2 cut(s) 127, 166
GluI GCNGC 2 cut(s) 199, 221
HaeII RGCGCY 1 cut(s) 129
HaeIII GGCC 4 cut(s) 73, 97, 142, 189
HapII CCGG 2 cut(s) 87, 98
HhaI GCGC 2 cut(s) 128, 167
Hin1II CATG 3 cut(s) 10, 58, 114
Hin6I GCGC 2 cut(s) 126, 165
HinP1I GCGC 2 cut(s) 126, 165
HinfI GANTC 1 cut(s) 116
HpaII CCGG 2 cut(s) 87, 98
Hpy166II GTNNAC 1 cut(s) 17
Hpy188I TCNGA 2 cut(s) 105, 123
Hpy188III TCNNGA 1 cut(s) 209
Hpy8I GTNNAC 1 cut(s) 17
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 4 cut(s) 43, 47, 87, 179
HpyCH4V TGCA 2 cut(s) 147, 237
HpyF10VI GCNNNNNNNGC 2 cut(s) 195, 207
Hsp92II CATG 3 cut(s) 10, 58, 114
HspAI GCGC 2 cut(s) 126, 165
Kzo9I GATC 2 cut(s) 10, 100
LpnPI CCDG 6 cut(s) 32, 59, 87, 100, 111, 202
Lsp1109I GCAGC 1 cut(s) 207
MalI GATC 2 cut(s) 12, 102
MboI GATC 2 cut(s) 10, 100
MboII GAAGA 1 cut(s) 118
MflI RGATCY 1 cut(s) 100
MlyI GAGTC 1 cut(s) 125
MnlI CCTC 2 cut(s) 83, 130
MspI CCGG 2 cut(s) 87, 98
MspR9I CCNGG 1 cut(s) 47
MvaI CCWGG 1 cut(s) 47
MvnI CGCG 1 cut(s) 210
MwoI GCNNNNNNNGC 2 cut(s) 195, 207
NdeII GATC 2 cut(s) 10, 100
NlaIII CATG 3 cut(s) 10, 58, 114
NlaIV GGNNCC 3 cut(s) 72, 102, 141
NruI TCGCGA 1 cut(s) 210
NsbI TGCGCA 1 cut(s) 166
NspI RCATGY 1 cut(s) 114
PkrI GCNGC 2 cut(s) 200, 222
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
Psp6I CCWGG 1 cut(s) 45
PspGI CCWGG 1 cut(s) 45
PspN4I GGNNCC 3 cut(s) 72, 102, 141
PspPI GGNCC 2 cut(s) 71, 140
PsuI RGATCY 1 cut(s) 100
RruI TCGCGA 1 cut(s) 210
SatI GCNGC 2 cut(s) 199, 221
Sau3AI GATC 2 cut(s) 10, 100
Sau96I GGNCC 2 cut(s) 71, 140
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 1 cut(s) 47
SetI ASST 1 cut(s) 247
SfcI CTRYAG 1 cut(s) 60
SsiI CCGC 1 cut(s) 198
StyD4I CCNGG 1 cut(s) 45
TauI GCSGC 1 cut(s) 201
TseI GCWGC 1 cut(s) 220
TspDTI ATGAA 1 cut(s) 17
XceI RCATGY 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.