Belongs to the DEFL family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 30 |
| AgsI | TTSAA | 3 cut(s) | 12, 27, 151 |
| Alw21I | GWGCWC | 1 cut(s) | 251 |
| Alw44I | GTGCAC | 1 cut(s) | 247 |
| AoxI | GGCC | 2 cut(s) | 71, 214 |
| ApaLI | GTGCAC | 1 cut(s) | 247 |
| ApeKI | GCWGC | 1 cut(s) | 56 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 83, 115 |
| AspS9I | GGNCC | 1 cut(s) | 71 |
| AsuHPI | GGTGA | 1 cut(s) | 199 |
| BaeGI | GKGCMC | 1 cut(s) | 251 |
| BauI | CACGAG | 1 cut(s) | 234 |
| Bbv12I | GWGCWC | 1 cut(s) | 251 |
| BbvI | GCAGC | 1 cut(s) | 43 |
| BccI | CCATC | 1 cut(s) | 61 |
| BfmI | CTRYAG | 1 cut(s) | 190 |
| BglII | AGATCT | 1 cut(s) | 123 |
| BisI | GCNGC | 1 cut(s) | 57 |
| BlsI | GCNGC | 1 cut(s) | 58 |
| BmgT120I | GGNCC | 1 cut(s) | 71 |
| BmiI | GGNNCC | 2 cut(s) | 72, 135 |
| BmsI | GCATC | 1 cut(s) | 25 |
| BseSI | GKGCMC | 1 cut(s) | 251 |
| BseXI | GCAGC | 1 cut(s) | 43 |
| BshFI | GGCC | 2 cut(s) | 73, 216 |
| BsiHKAI | GWGCWC | 1 cut(s) | 251 |
| BsnI | GGCC | 2 cut(s) | 73, 216 |
| Bsp1286I | GDGCHC | 1 cut(s) | 251 |
| Bsp143I | GATC | 1 cut(s) | 123 |
| BspACI | CCGC | 1 cut(s) | 30 |
| BspANI | GGCC | 2 cut(s) | 73, 216 |
| BspLI | GGNNCC | 2 cut(s) | 72, 135 |
| BssMI | GATC | 1 cut(s) | 123 |
| BssSI | CACGAG | 1 cut(s) | 234 |
| Bst2BI | CACGAG | 1 cut(s) | 234 |
| Bst4CI | ACNGT | 1 cut(s) | 179 |
| BstC8I | GCNNGC | 1 cut(s) | 241 |
| BstKTI | GATC | 1 cut(s) | 126 |
| BstMBI | GATC | 1 cut(s) | 123 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 13 |
| BstSFI | CTRYAG | 1 cut(s) | 190 |
| BstSLI | GKGCMC | 1 cut(s) | 251 |
| BstV1I | GCAGC | 1 cut(s) | 43 |
| BstX2I | RGATCY | 1 cut(s) | 123 |
| BstYI | RGATCY | 1 cut(s) | 123 |
| BsuRI | GGCC | 2 cut(s) | 73, 216 |
| Cac8I | GCNNGC | 1 cut(s) | 241 |
| Cfr13I | GGNCC | 1 cut(s) | 71 |
| CviJI | RGCY | 3 cut(s) | 56, 73, 216 |
| CviKI_1 | RGCY | 3 cut(s) | 56, 73, 216 |
| DpnI | GATC | 1 cut(s) | 125 |
| DpnII | GATC | 1 cut(s) | 123 |
| EcoT22I | ATGCAT | 1 cut(s) | 18 |
| Fnu4HI | GCNGC | 1 cut(s) | 57 |
| Fsp4HI | GCNGC | 1 cut(s) | 57 |
| GluI | GCNGC | 1 cut(s) | 57 |
| HaeIII | GGCC | 2 cut(s) | 73, 216 |
| HinfI | GANTC | 2 cut(s) | 98, 232 |
| HphI | GGTGA | 1 cut(s) | 199 |
| Hpy166II | GTNNAC | 2 cut(s) | 182, 249 |
| Hpy8I | GTNNAC | 2 cut(s) | 182, 249 |
| HpyAV | CCTTC | 1 cut(s) | 43 |
| HpyCH4III | ACNGT | 1 cut(s) | 179 |
| HpyCH4V | TGCA | 2 cut(s) | 16, 249 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 13 |
| Kzo9I | GATC | 1 cut(s) | 123 |
| LmnI | GCTCC | 2 cut(s) | 4, 199 |
| LpnPI | CCDG | 3 cut(s) | 158, 202, 223 |
| Lsp1109I | GCAGC | 1 cut(s) | 43 |
| LweI | GCATC | 1 cut(s) | 25 |
| MalI | GATC | 1 cut(s) | 125 |
| MboI | GATC | 1 cut(s) | 123 |
| MboII | GAAGA | 1 cut(s) | 133 |
| MflI | RGATCY | 1 cut(s) | 123 |
| MhlI | GDGCHC | 1 cut(s) | 251 |
| MluCI | AATT | 1 cut(s) | 203 |
| MlyI | GAGTC | 2 cut(s) | 107, 226 |
| MnlI | CCTC | 4 cut(s) | 79, 109, 124, 206 |
| Mph1103I | ATGCAT | 1 cut(s) | 18 |
| MslI | CAYNNNNRTG | 1 cut(s) | 222 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 13 |
| NdeII | GATC | 1 cut(s) | 123 |
| NlaIV | GGNNCC | 2 cut(s) | 72, 135 |
| NsiI | ATGCAT | 1 cut(s) | 18 |
| PkrI | GCNGC | 1 cut(s) | 58 |
| PleI | GAGTC | 2 cut(s) | 106, 226 |
| PpsI | GAGTC | 2 cut(s) | 106, 226 |
| PspN4I | GGNNCC | 2 cut(s) | 72, 135 |
| PspPI | GGNCC | 1 cut(s) | 71 |
| PsuI | RGATCY | 1 cut(s) | 123 |
| RseI | CAYNNNNRTG | 1 cut(s) | 222 |
| SatI | GCNGC | 1 cut(s) | 57 |
| Sau3AI | GATC | 1 cut(s) | 123 |
| Sau96I | GGNCC | 1 cut(s) | 71 |
| SchI | GAGTC | 2 cut(s) | 107, 226 |
| SduI | GDGCHC | 1 cut(s) | 251 |
| SetI | ASST | 2 cut(s) | 135, 191 |
| SfaNI | GCATC | 1 cut(s) | 25 |
| SfcI | CTRYAG | 1 cut(s) | 190 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 222 |
| Sse9I | AATT | 1 cut(s) | 203 |
| SsiI | CCGC | 1 cut(s) | 30 |
| TaaI | ACNGT | 1 cut(s) | 179 |
| TasI | AATT | 1 cut(s) | 203 |
| TseI | GCWGC | 1 cut(s) | 56 |
| VneI | GTGCAC | 1 cut(s) | 247 |
| Zsp2I | ATGCAT | 1 cut(s) | 18 |