MD14G1061400.v1.1

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001299
Physical Location & Seq
Reverse (-)
6462818 .. 6463441
624 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1061400.v1.1.491

Sequence Viewer

Length: 273 bp
ATGGAGCGTTCCGAGCGTTTGTTTTCGACTGTCTTCATCCTCCTCCTCCTTCTGGTGGCTATTGGAACGGATCCAATGGTTGCTGAGGGGAAGACAAAAGAGAAGTCATCGAAGAATGAGAAGTCGTCGAAGACATGTGAGTCTCCAAGCCATAAATACCACGGTATTTGCTTCAGGTCAAGCAATTGTGCAACTACATGCAAAAAAGAGGGATTTATGGGAGGCAAATGTACTGGCTTCTGGATGAGATGTGTATGCACTAAAAAATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

91

Amino Acids

10.04

Weight (kDa)

9.24

Isoelectric Point (pI)

46.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 44 - 90 8.2e-16 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 139
AclWI GGATC 2 cut(s) 65, 78
AcuI CTGAAG 1 cut(s) 157
AfaI GTAC 1 cut(s) 232
AfiI CCNNNNNNNGG 2 cut(s) 52, 55
AflIII ACRYGT 1 cut(s) 134
Alw26I GTCTC 1 cut(s) 147
AlwI GGATC 2 cut(s) 65, 78
ArsI GACNNNNNNTTYG 2 cut(s) 89, 121
BamHI GGATCC 1 cut(s) 70
BbsI GAAGAC 3 cut(s) 25, 98, 137
BbvCI CCTCAGC 1 cut(s) 84
BcoDI GTCTC 1 cut(s) 147
BmiI GGNNCC 1 cut(s) 72
BpiI GAAGAC 3 cut(s) 25, 98, 137
Bpu10I CCTNAGC 1 cut(s) 84
BsaJI CCNNGG 1 cut(s) 160
Bsc4I CCNNNNNNNGG 2 cut(s) 52, 55
Bse1I ACTGG 1 cut(s) 238
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 2 cut(s) 36, 249
BseLI CCNNNNNNNGG 2 cut(s) 52, 55
BseMII CTCAG 1 cut(s) 75
BseNI ACTGG 1 cut(s) 238
BseRI GAGGAG 2 cut(s) 32, 35
BslI CCNNNNNNNGG 2 cut(s) 52, 55
BsmAI GTCTC 1 cut(s) 147
Bsp143I GATC 1 cut(s) 70
BspCNI CTCAG 1 cut(s) 76
BspLI GGNNCC 1 cut(s) 72
BspPI GGATC 2 cut(s) 65, 78
BsrI ACTGG 1 cut(s) 238
BssECI CCNNGG 1 cut(s) 160
BssMI GATC 1 cut(s) 70
Bst4CI ACNGT 2 cut(s) 31, 164
BstDEI CTNAG 1 cut(s) 84
BstDSI CCRYGG 1 cut(s) 160
BstF5I GGATG 2 cut(s) 36, 249
BstKTI GATC 1 cut(s) 73
BstMAI GTCTC 1 cut(s) 147
BstMBI GATC 1 cut(s) 70
BstMWI GCNNNNNNNGC 1 cut(s) 13
BstNSI RCATGY 2 cut(s) 138, 201
BstV2I GAAGAC 3 cut(s) 25, 98, 137
BstX2I RGATCY 1 cut(s) 70
BstYI RGATCY 1 cut(s) 70
BtgI CCRYGG 1 cut(s) 160
BtsCI GGATG 2 cut(s) 36, 249
Csp6I GTAC 1 cut(s) 231
CspCI CAANNNNNGTGG 2 cut(s) 149, 184
CviAII CATG 2 cut(s) 135, 198
CviJI RGCY 3 cut(s) 59, 150, 237
CviKI_1 RGCY 3 cut(s) 59, 150, 237
CviQI GTAC 1 cut(s) 231
DdeI CTNAG 1 cut(s) 84
DpnI GATC 1 cut(s) 72
DpnII GATC 1 cut(s) 70
DrdI GACNNNNNNGTC 1 cut(s) 139
DseDI GACNNNNNNGTC 1 cut(s) 139
Eco57I CTGAAG 1 cut(s) 157
FaeI CATG 2 cut(s) 138, 201
FaiI YATR 5 cut(s) 136, 153, 199, 218, 256
FatI CATG 2 cut(s) 134, 197
FokI GGATG 2 cut(s) 23, 256
Hin1II CATG 2 cut(s) 138, 201
HinfI GANTC 1 cut(s) 140
Hpy188I TCNGA 1 cut(s) 13
Hpy188III TCNNGA 1 cut(s) 241
Hpy99I CGWCG 1 cut(s) 130
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 2 cut(s) 31, 164
HpyCH4V TGCA 3 cut(s) 191, 201, 258
HpyF10VI GCNNNNNNNGC 1 cut(s) 13
HpyF3I CTNAG 1 cut(s) 84
Hsp92II CATG 2 cut(s) 138, 201
Kzo9I GATC 1 cut(s) 70
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 4 cut(s) 38, 160, 219, 226
MalI GATC 1 cut(s) 72
MboI GATC 1 cut(s) 70
MboII GAAGA 4 cut(s) 25, 103, 124, 142
MfeI CAATTG 1 cut(s) 184
MflI RGATCY 1 cut(s) 70
MluCI AATT 1 cut(s) 184
MlyI GAGTC 1 cut(s) 149
MnlI CCTC 6 cut(s) 50, 53, 56, 79, 202, 215
MseI TTAA 1 cut(s) 271
MunI CAATTG 1 cut(s) 184
MwoI GCNNNNNNNGC 1 cut(s) 13
NdeII GATC 1 cut(s) 70
NlaIII CATG 2 cut(s) 138, 201
NlaIV GGNNCC 1 cut(s) 72
NspI RCATGY 2 cut(s) 138, 201
PciI ACATGT 1 cut(s) 134
PleI GAGTC 1 cut(s) 148
PpsI GAGTC 1 cut(s) 148
PscI ACATGT 1 cut(s) 134
PspN4I GGNNCC 1 cut(s) 72
PsuI RGATCY 1 cut(s) 70
RsaI GTAC 1 cut(s) 232
RsaNI GTAC 1 cut(s) 231
SaqAI TTAA 1 cut(s) 271
Sau3AI GATC 1 cut(s) 70
SchI GAGTC 1 cut(s) 149
SetI ASST 1 cut(s) 179
Sse9I AATT 1 cut(s) 184
TaaI ACNGT 2 cut(s) 31, 164
TaqI TCGA 3 cut(s) 26, 110, 128
TasI AATT 1 cut(s) 184
TatI WGTACW 1 cut(s) 230
Tru1I TTAA 1 cut(s) 271
Tru9I TTAA 1 cut(s) 271
TspDTI ATGAA 1 cut(s) 25
TspGWI ACGGA 1 cut(s) 83
XceI RCATGY 2 cut(s) 138, 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.