MD16G1008900.v1.1

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001299
Physical Location & Seq
Reverse (-)
652130 .. 652978
849 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1008900.v1.1.491

Sequence Viewer

Length: 264 bp
ATGGAGCATTCAATGCGCCTTGTTTCTGCTGCCTTCGTTCTTGTCCTGCTTTTGGCCACTACCGAGATGGGGCCAATGGGTGTTGAGGCAAAGTCCAAATCAAGCAAGGAAGTTGAGAAAAGAACCTGTGAGGCTGCGAGCGGCAAGTTCAAGGGGATGTGCTTTTCGTCTAACAACTGTGCAAACACCTGCGCAAGAGAGAAATTCGATGGAGGCAAATGCAAGGGCTTCCGCCGTAGATGCATGTGCACCAAAAAGTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

88

Amino Acids

9.54

Weight (kDa)

9.41

Isoelectric Point (pI)

34.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 41 - 87 3.3e-16 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 197
Acc16I TGCGCA 1 cut(s) 193
Acc36I ACCTGC 1 cut(s) 197
AccBSI CCGCTC 1 cut(s) 141
AciI CCGC 2 cut(s) 141, 232
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 1 cut(s) 203
AfiI CCNNNNNNNGG 2 cut(s) 52, 69
AgsI TTSAA 2 cut(s) 12, 151
Alw21I GWGCWC 1 cut(s) 251
Alw44I GTGCAC 1 cut(s) 247
AoxI GGCC 2 cut(s) 54, 71
ApaLI GTGCAC 1 cut(s) 247
ApeKI GCWGC 2 cut(s) 29, 134
ApoI RAATTY 1 cut(s) 203
AspLEI GCGC 2 cut(s) 18, 194
AspS9I GGNCC 1 cut(s) 71
BaeGI GKGCMC 1 cut(s) 251
BalI TGGCCA 1 cut(s) 56
Bbv12I GWGCWC 1 cut(s) 251
BbvI GCAGC 2 cut(s) 16, 121
BccI CCATC 2 cut(s) 61, 203
BceAI ACGGC 1 cut(s) 219
BfuAI ACCTGC 1 cut(s) 197
BisI GCNGC 3 cut(s) 30, 135, 142
BlsI GCNGC 3 cut(s) 31, 136, 143
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 1 cut(s) 72
BmsI GCATC 1 cut(s) 230
Bsc4I CCNNNNNNNGG 2 cut(s) 52, 69
BseGI GGATG 1 cut(s) 162
BseLI CCNNNNNNNGG 2 cut(s) 52, 69
BseSI GKGCMC 1 cut(s) 251
BseXI GCAGC 2 cut(s) 16, 121
BshFI GGCC 2 cut(s) 56, 73
BsiHKAI GWGCWC 1 cut(s) 251
BslI CCNNNNNNNGG 2 cut(s) 52, 69
BsmI GAATGC 1 cut(s) 7
BsnI GGCC 2 cut(s) 56, 73
Bsp1286I GDGCHC 1 cut(s) 251
BspACI CCGC 2 cut(s) 141, 232
BspANI GGCC 2 cut(s) 56, 73
BspLI GGNNCC 1 cut(s) 72
BspMI ACCTGC 1 cut(s) 197
BsrBI CCGCTC 1 cut(s) 141
Bst4CI ACNGT 1 cut(s) 179
BstAPI GCANNNNNTGC 1 cut(s) 13
BstC8I GCNNGC 1 cut(s) 139
BstF5I GGATG 1 cut(s) 162
BstHHI GCGC 2 cut(s) 18, 194
BstMWI GCNNNNNNNGC 2 cut(s) 13, 240
BstNSI RCATGY 1 cut(s) 247
BstSLI GKGCMC 1 cut(s) 251
BstV1I GCAGC 2 cut(s) 16, 121
BsuRI GGCC 2 cut(s) 56, 73
BtsCI GGATG 1 cut(s) 162
BveI ACCTGC 1 cut(s) 197
Cac8I GCNNGC 1 cut(s) 139
CfoI GCGC 2 cut(s) 18, 194
Cfr13I GGNCC 1 cut(s) 71
CviAII CATG 1 cut(s) 244
CviJI RGCY 4 cut(s) 56, 73, 134, 228
CviKI_1 RGCY 4 cut(s) 56, 73, 134, 228
EaeI YGGCCR 1 cut(s) 54
EciI GGCGGA 1 cut(s) 221
EcoT22I ATGCAT 1 cut(s) 245
FaeI CATG 1 cut(s) 247
FaiI YATR 1 cut(s) 245
FatI CATG 1 cut(s) 243
Fnu4HI GCNGC 3 cut(s) 30, 135, 142
FokI GGATG 1 cut(s) 169
Fsp4HI GCNGC 3 cut(s) 30, 135, 142
FspI TGCGCA 1 cut(s) 193
GlaI GCGC 2 cut(s) 17, 193
GluI GCNGC 3 cut(s) 30, 135, 142
HaeIII GGCC 2 cut(s) 56, 73
HhaI GCGC 2 cut(s) 18, 194
Hin1II CATG 1 cut(s) 247
Hin6I GCGC 2 cut(s) 16, 192
HinP1I GCGC 2 cut(s) 16, 192
Hpy166II GTNNAC 1 cut(s) 249
Hpy8I GTNNAC 1 cut(s) 249
HpyAV CCTTC 1 cut(s) 43
HpyCH4III ACNGT 1 cut(s) 179
HpyCH4V TGCA 4 cut(s) 182, 222, 243, 249
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 240
Hsp92II CATG 1 cut(s) 247
HspAI GCGC 2 cut(s) 16, 192
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 3 cut(s) 59, 139, 202
Lsp1109I GCAGC 2 cut(s) 16, 121
LweI GCATC 1 cut(s) 230
MbiI CCGCTC 1 cut(s) 141
MhlI GDGCHC 1 cut(s) 251
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 1 cut(s) 203
MluNI TGGCCA 1 cut(s) 56
MnlI CCTC 3 cut(s) 79, 124, 206
Mox20I TGGCCA 1 cut(s) 56
Mph1103I ATGCAT 1 cut(s) 245
MscI TGGCCA 1 cut(s) 56
Msp20I TGGCCA 1 cut(s) 56
Mva1269I GAATGC 1 cut(s) 7
MwoI GCNNNNNNNGC 2 cut(s) 13, 240
NlaIII CATG 1 cut(s) 247
NlaIV GGNNCC 1 cut(s) 72
NsbI TGCGCA 1 cut(s) 193
NsiI ATGCAT 1 cut(s) 245
NspI RCATGY 1 cut(s) 247
PaqCI CACCTGC 1 cut(s) 197
PctI GAATGC 1 cut(s) 7
PkrI GCNGC 3 cut(s) 31, 136, 143
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 71
SatI GCNGC 3 cut(s) 30, 135, 142
Sau96I GGNCC 1 cut(s) 71
SduI GDGCHC 1 cut(s) 251
SetI ASST 2 cut(s) 128, 191
SfaNI GCATC 1 cut(s) 230
Sse9I AATT 1 cut(s) 203
SsiI CCGC 2 cut(s) 141, 232
TaaI ACNGT 1 cut(s) 179
TaqI TCGA 1 cut(s) 207
TasI AATT 1 cut(s) 203
TauI GCSGC 1 cut(s) 144
TseI GCWGC 2 cut(s) 29, 134
VneI GTGCAC 1 cut(s) 247
XapI RAATTY 1 cut(s) 203
XceI RCATGY 1 cut(s) 247
XcmI CCANNNNNNNNNTGG 1 cut(s) 64
Zsp2I ATGCAT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.