Rorug03G0183000

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001299
Physical Location & Seq
Forward (+)
15740243 .. 15741321
1079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0183000.1

Sequence Viewer

Length: 702 bp
ATGAGCATTGGGCCACCGAATGTTGGTGGCACCTCCATAACAACTCACCGATCAAATTACACGGCTTCAAGTGGAGCTGAACAATATGGACTTGTGCTTATCCAAAAACTAATAGACCTACATGACAAGAAAAAACTTGCCCGTAGGCTGCTTTTCATTCGGAGTGGGAGTGGGAGTACTGAAGACCGTGAGAAAAGTCTTCTGACAAAGCTGAAGCAGCAATGTGGTGGACAATTCACCTCCAAGATGGAAGGAATGATCACCGATTTGACAGTGGATAAAGAATCACTCACCAGATTTGGAGCGTTCATCCAAGAAAAGAATCGAAATCCCGGGCTCAATTTATCAGTCCAGATTCTCACAACCGGTTACTGGCCAAGTTACAAATCGTCTGATCTCAACCTTCCTGAAGAGATCGATGGGAATACCAGCGCAAAAAGCTGTCATGCATGGATATACTCACTGGGTTCTTCCAACGTCGAGGGCAAGTTTCATTCGAAAACCATAGAAGTGGTTGTGACAACTCATCAGGCTGCTCTTCTGCTGCTCTTTAACAATGCCAAGAAGTTGAGCTTTTCAGAAATCTCAATTCAGCTAAATCTAACCAAAGACGAGTTGATTAGACTTGCTTCCTCCTCTTTCATGTGCGAAGTACAAGATCCTTCTCAAGGAGCCAAGTACAAAGACTATCTCGCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009505 GO:0016020 GO:0030312 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

233

Amino Acids

25.74

Weight (kDa)

8.65

Isoelectric Point (pI)

36.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin PF00888 43 - 95 3.9e-14 Cullin alpha solenoid domain
Cullin_AB PF26557 119 - 209 3.8e-13 Cullin alpha+beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 29
AclWI GGATC 1 cut(s) 653
AcoI YGGCCR 1 cut(s) 374
AcuI CTGAAG 3 cut(s) 201, 233, 429
AfaI GTAC 3 cut(s) 178, 654, 680
AfiI CCNNNNNNNGG 3 cut(s) 23, 372, 668
AgeI ACCGGT 1 cut(s) 365
AgsI TTSAA 1 cut(s) 69
AluBI AGCT 5 cut(s) 77, 211, 441, 573, 595
AluI AGCT 5 cut(s) 77, 211, 441, 573, 595
AlwI GGATC 1 cut(s) 653
Ama87I CYCGRG 1 cut(s) 332
AoxI GGCC 2 cut(s) 11, 374
ApeKI GCWGC 4 cut(s) 148, 217, 533, 544
AsiGI ACCGGT 1 cut(s) 365
AspLEI GCGC 1 cut(s) 434
AspS9I GGNCC 1 cut(s) 11
AsuC2I CCSGG 2 cut(s) 333, 334
AsuHPI GGTGA 4 cut(s) 38, 229, 253, 283
AsuII TTCGAA 1 cut(s) 497
AvaI CYCGRG 1 cut(s) 332
BalI TGGCCA 1 cut(s) 376
BanI GGYRCC 1 cut(s) 29
BanII GRGCYC 1 cut(s) 339
BbsI GAAGAC 2 cut(s) 189, 191
BbvI GCAGC 4 cut(s) 135, 229, 520, 531
BccI CCATC 2 cut(s) 241, 413
BceAI ACGGC 1 cut(s) 78
BclI TGATCA 1 cut(s) 258
BcnI CCSGG 2 cut(s) 333, 334
BisI GCNGC 4 cut(s) 149, 218, 534, 545
BlsI GCNGC 4 cut(s) 150, 219, 535, 546
BmcAI AGTACT 1 cut(s) 178
Bme1390I CCNGG 2 cut(s) 333, 334
BmeT110I CYCGRG 1 cut(s) 332
BmgT120I GGNCC 1 cut(s) 11
BmiI GGNNCC 2 cut(s) 31, 673
BmrFI CCNGG 2 cut(s) 333, 334
BmrI ACTGGG 1 cut(s) 473
BmuI ACTGGG 1 cut(s) 473
BpiI GAAGAC 2 cut(s) 189, 191
Bpu14I TTCGAA 1 cut(s) 497
BpuEI CTTGAG 1 cut(s) 651
BpuMI CCSGG 2 cut(s) 333, 334
Bsa29I ATCGAT 1 cut(s) 417
BsaJI CCNNGG 1 cut(s) 332
BsaWI WCCGGW 1 cut(s) 365
BsaXI ACNNNNNCTCC 4 cut(s) 154, 160, 184, 190
Bsc4I CCNNNNNNNGG 3 cut(s) 23, 372, 668
Bse118I RCCGGY 1 cut(s) 365
Bse1I ACTGG 2 cut(s) 377, 468
Bse3DI GCAATG 1 cut(s) 227
BseCI ATCGAT 1 cut(s) 417
BseDI CCNNGG 1 cut(s) 332
BseGI GGATG 1 cut(s) 309
BseLI CCNNNNNNNGG 3 cut(s) 23, 372, 668
BseMI GCAATG 1 cut(s) 227
BseNI ACTGG 2 cut(s) 377, 468
BseRI GAGGAG 1 cut(s) 625
BseXI GCAGC 4 cut(s) 135, 229, 520, 531
BshFI GGCC 2 cut(s) 13, 376
BshNI GGYRCC 1 cut(s) 29
BshTI ACCGGT 1 cut(s) 365
BshVI ATCGAT 1 cut(s) 417
BsiHKCI CYCGRG 1 cut(s) 332
BsiSI CCGG 2 cut(s) 333, 366
BslI CCNNNNNNNGG 3 cut(s) 23, 372, 668
BsnI GGCC 2 cut(s) 13, 376
BsoBI CYCGRG 1 cut(s) 332
Bsp119I TTCGAA 1 cut(s) 497
Bsp1286I GDGCHC 1 cut(s) 339
Bsp143I GATC 5 cut(s) 50, 258, 394, 414, 658
BspANI GGCC 2 cut(s) 13, 376
BspDI ATCGAT 1 cut(s) 417
BspLI GGNNCC 2 cut(s) 31, 673
BspPI GGATC 1 cut(s) 653
BspQI GCTCTTC 1 cut(s) 543
BspT104I TTCGAA 1 cut(s) 497
BspT107I GGYRCC 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 227
BsrFI RCCGGY 1 cut(s) 365
BsrI ACTGG 2 cut(s) 377, 468
BssAI RCCGGY 1 cut(s) 365
BssECI CCNNGG 1 cut(s) 332
BssMI GATC 5 cut(s) 50, 258, 394, 414, 658
Bst4CI ACNGT 2 cut(s) 188, 274
Bst6I CTCTTC 2 cut(s) 405, 543
BstBI TTCGAA 1 cut(s) 497
BstENI CCTNNNNNAGG 1 cut(s) 666
BstF5I GGATG 1 cut(s) 309
BstHHI GCGC 1 cut(s) 434
BstKTI GATC 5 cut(s) 53, 261, 397, 417, 661
BstMBI GATC 5 cut(s) 50, 258, 394, 414, 658
BstMWI GCNNNNNNNGC 2 cut(s) 217, 438
BstSCI CCNGG 2 cut(s) 331, 332
BstV1I GCAGC 4 cut(s) 135, 229, 520, 531
BstV2I GAAGAC 2 cut(s) 189, 191
BstX2I RGATCY 1 cut(s) 658
BstXI CCANNNNNNTGG 1 cut(s) 511
BstYI RGATCY 1 cut(s) 658
Bsu15I ATCGAT 1 cut(s) 417
BsuRI GGCC 2 cut(s) 13, 376
BsuTUI ATCGAT 1 cut(s) 417
BtsCI GGATG 1 cut(s) 309
BtsIMutI CAGTG 2 cut(s) 279, 461
CfoI GCGC 1 cut(s) 434
Cfr10I RCCGGY 1 cut(s) 365
Cfr13I GGNCC 1 cut(s) 11
Cfr9I CCCGGG 1 cut(s) 332
ClaI ATCGAT 1 cut(s) 417
Csp6I GTAC 3 cut(s) 177, 653, 679
CspAI ACCGGT 1 cut(s) 365
CviAII CATG 4 cut(s) 122, 446, 450, 643
CviQI GTAC 3 cut(s) 177, 653, 679
DpnI GATC 5 cut(s) 52, 260, 396, 416, 660
DpnII GATC 5 cut(s) 50, 258, 394, 414, 658
EaeI YGGCCR 1 cut(s) 374
Eam1104I CTCTTC 2 cut(s) 405, 543
EarI CTCTTC 2 cut(s) 405, 543
Eco24I GRGCYC 1 cut(s) 339
Eco57I CTGAAG 3 cut(s) 201, 233, 429
Eco88I CYCGRG 1 cut(s) 332
EcoNI CCTNNNNNAGG 1 cut(s) 666
EcoT22I ATGCAT 1 cut(s) 451
EcoT38I GRGCYC 1 cut(s) 339
FaeI CATG 4 cut(s) 125, 449, 453, 646
FaiI YATR 8 cut(s) 38, 87, 123, 447, 451, 457, 506, 644
FalI AAGNNNNNCTT 2 cut(s) 557, 589
FatI CATG 4 cut(s) 121, 445, 449, 642
FbaI TGATCA 1 cut(s) 258
Fnu4HI GCNGC 4 cut(s) 149, 218, 534, 545
FokI GGATG 1 cut(s) 296
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 4 cut(s) 149, 218, 534, 545
GlaI GCGC 1 cut(s) 433
GluI GCNGC 4 cut(s) 149, 218, 534, 545
HaeIII GGCC 2 cut(s) 13, 376
HapII CCGG 2 cut(s) 333, 366
HhaI GCGC 1 cut(s) 434
Hin1II CATG 4 cut(s) 125, 449, 453, 646
Hin6I GCGC 1 cut(s) 432
HinP1I GCGC 1 cut(s) 432
HinfI GANTC 3 cut(s) 284, 322, 355
HpaII CCGG 2 cut(s) 333, 366
HphI GGTGA 4 cut(s) 38, 229, 253, 283
Hpy166II GTNNAC 1 cut(s) 230
Hpy188I TCNGA 4 cut(s) 162, 204, 394, 580
Hpy188III TCNNGA 2 cut(s) 352, 407
Hpy8I GTNNAC 1 cut(s) 230
Hpy99I CGWCG 1 cut(s) 482
HpyAV CCTTC 3 cut(s) 245, 413, 672
HpyCH4III ACNGT 2 cut(s) 188, 274
HpyCH4IV ACGT 1 cut(s) 477
HpyCH4V TGCA 1 cut(s) 449
HpyF10VI GCNNNNNNNGC 2 cut(s) 217, 438
HpySE526I ACGT 1 cut(s) 477
Hsp92II CATG 4 cut(s) 125, 449, 453, 646
HspAI GCGC 1 cut(s) 432
Ksp22I TGATCA 1 cut(s) 258
Kzo9I GATC 5 cut(s) 50, 258, 394, 414, 658
LguI GCTCTTC 1 cut(s) 543
LmnI GCTCC 3 cut(s) 74, 302, 671
LpnPI CCDG 9 cut(s) 307, 346, 358, 365, 379, 420, 442, 449, 515
Lsp1109I GCAGC 4 cut(s) 135, 229, 520, 531
MaeII ACGT 1 cut(s) 477
MaeIII GTNAC 3 cut(s) 368, 380, 517
MalI GATC 5 cut(s) 52, 260, 396, 416, 660
MboI GATC 5 cut(s) 50, 258, 394, 414, 658
MboII GAAGA 5 cut(s) 191, 194, 422, 462, 530
MflI RGATCY 1 cut(s) 658
MhlI GDGCHC 1 cut(s) 339
MlsI TGGCCA 1 cut(s) 376
MluCI AATT 4 cut(s) 55, 233, 340, 588
MluNI TGGCCA 1 cut(s) 376
MmeI TCCRAC 1 cut(s) 498
MnlI CCTC 5 cut(s) 43, 250, 475, 643, 646
Mox20I TGGCCA 1 cut(s) 376
Mph1103I ATGCAT 1 cut(s) 451
MscI TGGCCA 1 cut(s) 376
MseI TTAA 1 cut(s) 552
MslI CAYNNNNRTG 1 cut(s) 509
Msp20I TGGCCA 1 cut(s) 376
MspI CCGG 2 cut(s) 333, 366
MspR9I CCNGG 2 cut(s) 333, 334
MwoI GCNNNNNNNGC 2 cut(s) 217, 438
NciI CCSGG 2 cut(s) 333, 334
NdeII GATC 5 cut(s) 50, 258, 394, 414, 658
NlaIII CATG 4 cut(s) 125, 449, 453, 646
NlaIV GGNNCC 2 cut(s) 31, 673
NmuCI GTSAC 1 cut(s) 517
NsiI ATGCAT 1 cut(s) 451
NspV TTCGAA 1 cut(s) 497
PciSI GCTCTTC 1 cut(s) 543
PfeI GAWTC 3 cut(s) 284, 322, 355
PinAI ACCGGT 1 cut(s) 365
PkrI GCNGC 4 cut(s) 150, 219, 535, 546
PspN4I GGNNCC 2 cut(s) 31, 673
PspPI GGNCC 1 cut(s) 11
PsuI RGATCY 1 cut(s) 658
RsaI GTAC 3 cut(s) 178, 654, 680
RsaNI GTAC 3 cut(s) 177, 653, 679
RseI CAYNNNNRTG 1 cut(s) 509
SapI GCTCTTC 1 cut(s) 543
SaqAI TTAA 1 cut(s) 552
SatI GCNGC 4 cut(s) 149, 218, 534, 545
Sau3AI GATC 5 cut(s) 50, 258, 394, 414, 658
Sau96I GGNCC 1 cut(s) 11
ScaI AGTACT 1 cut(s) 178
ScrFI CCNGG 2 cut(s) 333, 334
SduI GDGCHC 1 cut(s) 339
SfuI TTCGAA 1 cut(s) 497
SmaI CCCGGG 1 cut(s) 334
SmiMI CAYNNNNRTG 1 cut(s) 509
SmlI CTYRAG 1 cut(s) 666
SmoI CTYRAG 1 cut(s) 666
Sse9I AATT 4 cut(s) 55, 233, 340, 588
StyD4I CCNGG 2 cut(s) 331, 332
TaaI ACNGT 2 cut(s) 188, 274
TaiI ACGT 1 cut(s) 480
TaqI TCGA 4 cut(s) 325, 417, 480, 497
TasI AATT 4 cut(s) 55, 233, 340, 588
TatI WGTACW 3 cut(s) 176, 652, 678
TfiI GAWTC 3 cut(s) 284, 322, 355
Tru1I TTAA 1 cut(s) 552
Tru9I TTAA 1 cut(s) 552
TscAI CASTG 2 cut(s) 279, 468
TseFI GTSAC 1 cut(s) 517
TseI GCWGC 4 cut(s) 148, 217, 533, 544
Tsp45I GTSAC 1 cut(s) 517
TspDTI ATGAA 4 cut(s) 145, 298, 482, 631
TspMI CCCGGG 1 cut(s) 332
TspRI CASTG 2 cut(s) 279, 468
XagI CCTNNNNNAGG 1 cut(s) 666
XmaI CCCGGG 1 cut(s) 332
ZrmI AGTACT 1 cut(s) 178
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.