MD16G1008800.v1.1

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001299
Physical Location & Seq
Reverse (-)
649888 .. 650643
756 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1008800.v1.1.491

Sequence Viewer

Length: 273 bp
ATGGAGCCCTCAATGCGTTTTGTTTCAGCTGCTTTCGTTCTCATCTTGCTTTTGGTCACTACTGGGATGGGGCCAATGGCCGCTGAGGGAAGGAGTGGCATGACTAGCAAGGGAGCAGCTGAGAAAAAGAAGTGCGATTCTCATCCGAGTAGCAAGTTCAAGGGAGTGTGCTTCTTCACAAACAACTGCGTAGACACCTGCAAATTAGAGGGCTCCAGTGGAGGACAATGCCGTGGCTTCCGCCGTAGATGCATATGCACCAAACAATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

91

Amino Acids

9.68

Weight (kDa)

9.35

Isoelectric Point (pI)

35.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 45 - 90 2.5e-12 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 206
Acc36I ACCTGC 1 cut(s) 206
AccI GTMKAC 1 cut(s) 192
AciI CCGC 2 cut(s) 81, 241
AcoI YGGCCR 1 cut(s) 78
AgsI TTSAA 1 cut(s) 160
AluBI AGCT 2 cut(s) 29, 119
AluI AGCT 2 cut(s) 29, 119
AoxI GGCC 2 cut(s) 71, 78
ApeKI GCWGC 2 cut(s) 29, 116
AspS9I GGNCC 1 cut(s) 71
BanII GRGCYC 2 cut(s) 9, 215
BbvCI CCTCAGC 1 cut(s) 84
BbvI GCAGC 2 cut(s) 16, 128
BccI CCATC 1 cut(s) 61
BceAI ACGGC 2 cut(s) 216, 228
BfaI CTAG 1 cut(s) 105
BfuAI ACCTGC 1 cut(s) 206
BisI GCNGC 3 cut(s) 30, 81, 117
BlsI GCNGC 3 cut(s) 31, 82, 118
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 3 cut(s) 6, 72, 214
BmrI ACTGGG 1 cut(s) 72
BmsI GCATC 1 cut(s) 239
BmuI ACTGGG 1 cut(s) 72
BpmI CTGGAG 1 cut(s) 199
Bpu10I CCTNAGC 1 cut(s) 84
BsaBI GATNNNNATC 1 cut(s) 141
BsaJI CCNNGG 1 cut(s) 232
Bse1I ACTGG 2 cut(s) 67, 216
Bse8I GATNNNNATC 1 cut(s) 141
BseDI CCNNGG 1 cut(s) 232
BseGI GGATG 2 cut(s) 72, 142
BseJI GATNNNNATC 1 cut(s) 141
BseMII CTCAG 2 cut(s) 75, 111
BseNI ACTGG 2 cut(s) 67, 216
BseXI GCAGC 2 cut(s) 16, 128
BshFI GGCC 2 cut(s) 73, 80
BsnI GGCC 2 cut(s) 73, 80
Bsp1286I GDGCHC 2 cut(s) 9, 215
BspACI CCGC 2 cut(s) 81, 241
BspANI GGCC 2 cut(s) 73, 80
BspCNI CTCAG 2 cut(s) 76, 112
BspLI GGNNCC 3 cut(s) 6, 72, 214
BspMI ACCTGC 1 cut(s) 206
BsrI ACTGG 2 cut(s) 67, 216
BssECI CCNNGG 1 cut(s) 232
BstDEI CTNAG 2 cut(s) 84, 120
BstDSI CCRYGG 1 cut(s) 232
BstF5I GGATG 2 cut(s) 72, 142
BstMWI GCNNNNNNNGC 3 cut(s) 13, 105, 249
BstV1I GCAGC 2 cut(s) 16, 128
BsuRI GGCC 2 cut(s) 73, 80
BtgI CCRYGG 1 cut(s) 232
BtsCI GGATG 2 cut(s) 72, 142
BtsIMutI CAGTG 1 cut(s) 223
BveI ACCTGC 1 cut(s) 206
Cfr13I GGNCC 1 cut(s) 71
CviAII CATG 1 cut(s) 100
CviJI RGCY 7 cut(s) 7, 29, 73, 80, 119, 213, 237
CviKI_1 RGCY 7 cut(s) 7, 29, 73, 80, 119, 213, 237
DdeI CTNAG 2 cut(s) 84, 120
EaeI YGGCCR 1 cut(s) 78
EciI GGCGGA 1 cut(s) 230
Eco24I GRGCYC 2 cut(s) 9, 215
EcoT22I ATGCAT 1 cut(s) 254
EcoT38I GRGCYC 2 cut(s) 9, 215
FaeI CATG 1 cut(s) 103
FaiI YATR 3 cut(s) 101, 254, 256
FatI CATG 1 cut(s) 99
FauNDI CATATG 1 cut(s) 254
FblI GTMKAC 1 cut(s) 192
Fnu4HI GCNGC 3 cut(s) 30, 81, 117
FokI GGATG 2 cut(s) 79, 129
FriOI GRGCYC 2 cut(s) 9, 215
Fsp4HI GCNGC 3 cut(s) 30, 81, 117
FspBI CTAG 1 cut(s) 105
GluI GCNGC 3 cut(s) 30, 81, 117
GsuI CTGGAG 1 cut(s) 199
HaeIII GGCC 2 cut(s) 73, 80
Hin1II CATG 1 cut(s) 103
HinfI GANTC 1 cut(s) 137
Hpy166II GTNNAC 1 cut(s) 193
Hpy188I TCNGA 1 cut(s) 147
Hpy8I GTNNAC 1 cut(s) 193
HpyAV CCTTC 1 cut(s) 84
HpyCH4V TGCA 3 cut(s) 201, 252, 258
HpyF10VI GCNNNNNNNGC 3 cut(s) 13, 105, 249
HpyF3I CTNAG 2 cut(s) 84, 120
Hsp92II CATG 1 cut(s) 103
LmnI GCTCC 3 cut(s) 4, 113, 218
LpnPI CCDG 3 cut(s) 48, 211, 229
Lsp1109I GCAGC 2 cut(s) 16, 128
LweI GCATC 1 cut(s) 239
MaeI CTAG 1 cut(s) 105
MaeIII GTNAC 1 cut(s) 55
MboII GAAGA 1 cut(s) 166
MhlI GDGCHC 2 cut(s) 9, 215
MluCI AATT 1 cut(s) 203
MnlI CCTC 4 cut(s) 19, 79, 202, 215
Mph1103I ATGCAT 1 cut(s) 254
MspA1I CMGCKG 3 cut(s) 29, 83, 119
MwoI GCNNNNNNNGC 3 cut(s) 13, 105, 249
NdeI CATATG 1 cut(s) 254
NlaIII CATG 1 cut(s) 103
NlaIV GGNNCC 3 cut(s) 6, 72, 214
NmuCI GTSAC 1 cut(s) 55
NsiI ATGCAT 1 cut(s) 254
PaqCI CACCTGC 1 cut(s) 206
PfeI GAWTC 1 cut(s) 137
PkrI GCNGC 3 cut(s) 31, 82, 118
PspN4I GGNNCC 3 cut(s) 6, 72, 214
PspPI GGNCC 1 cut(s) 71
PvuII CAGCTG 2 cut(s) 29, 119
SatI GCNGC 3 cut(s) 30, 81, 117
Sau96I GGNCC 1 cut(s) 71
SduI GDGCHC 2 cut(s) 9, 215
SetI ASST 3 cut(s) 31, 121, 200
SfaNI GCATC 1 cut(s) 239
Sse9I AATT 1 cut(s) 203
SsiI CCGC 2 cut(s) 81, 241
SspMI CTAG 1 cut(s) 105
TasI AATT 1 cut(s) 203
TauI GCSGC 1 cut(s) 83
TfiI GAWTC 1 cut(s) 137
TscAI CASTG 1 cut(s) 223
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 2 cut(s) 29, 116
Tsp45I GTSAC 1 cut(s) 55
TspRI CASTG 1 cut(s) 223
XmiI GTMKAC 1 cut(s) 192
XspI CTAG 1 cut(s) 105
Zsp2I ATGCAT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.