pycom13g00970

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001299
Physical Location & Seq
Reverse (-)
622888 .. 623234
347 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g00970.1

Sequence Viewer

Length: 264 bp
ATGGAGCGTTCAATGCGTCTTGTTTCTGCTGCCTTCATTGTTGTTCTGCTTTTGGCCACTACCGAGATGGGGCCAATGAGTGTTGAGGCAAGGACTGAGTCAAGCAAGGTAGTTGAGGGAAAGATCTGCGAGATTCCGAGCACCTTGTTCAAGGGGTTGTGCTTTTCCTCTAACAATTGTAAACACACTTGCAGAAAGGAGCAATTCACCAGAGGAAAATGTAGTGGCTTGACCCGTACATGCATGTGCACCAAAAAGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

88

Amino Acids

9.63

Weight (kDa)

9.2

Isoelectric Point (pI)

32.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 41 - 87 2.4e-14 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 54
AfaI GTAC 1 cut(s) 238
AfiI CCNNNNNNNGG 1 cut(s) 69
AgsI TTSAA 2 cut(s) 12, 151
Alw21I GWGCWC 2 cut(s) 143, 251
Alw44I GTGCAC 1 cut(s) 247
AoxI GGCC 2 cut(s) 54, 71
ApaLI GTGCAC 1 cut(s) 247
ApeKI GCWGC 1 cut(s) 29
AspS9I GGNCC 1 cut(s) 71
AsuHPI GGTGA 1 cut(s) 199
BaeGI GKGCMC 1 cut(s) 251
BalI TGGCCA 1 cut(s) 56
Bbv12I GWGCWC 2 cut(s) 143, 251
BbvI GCAGC 1 cut(s) 16
BccI CCATC 1 cut(s) 61
BglII AGATCT 1 cut(s) 123
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 1 cut(s) 72
Bsc4I CCNNNNNNNGG 1 cut(s) 69
BseLI CCNNNNNNNGG 1 cut(s) 69
BseMII CTCAG 1 cut(s) 87
BseSI GKGCMC 1 cut(s) 251
BseXI GCAGC 1 cut(s) 16
BshFI GGCC 2 cut(s) 56, 73
BsiHKAI GWGCWC 2 cut(s) 143, 251
BslI CCNNNNNNNGG 1 cut(s) 69
BsnI GGCC 2 cut(s) 56, 73
Bsp1286I GDGCHC 2 cut(s) 143, 251
Bsp143I GATC 1 cut(s) 123
BspANI GGCC 2 cut(s) 56, 73
BspCNI CTCAG 1 cut(s) 88
BspLI GGNNCC 1 cut(s) 72
BssMI GATC 1 cut(s) 123
BstDEI CTNAG 1 cut(s) 96
BstKTI GATC 1 cut(s) 126
BstMBI GATC 1 cut(s) 123
BstMWI GCNNNNNNNGC 1 cut(s) 13
BstNSI RCATGY 2 cut(s) 243, 247
BstSLI GKGCMC 1 cut(s) 251
BstV1I GCAGC 1 cut(s) 16
BstX2I RGATCY 1 cut(s) 123
BstYI RGATCY 1 cut(s) 123
BsuRI GGCC 2 cut(s) 56, 73
Cfr13I GGNCC 1 cut(s) 71
CseI GACGC 1 cut(s) 5
Csp6I GTAC 1 cut(s) 237
CviAII CATG 2 cut(s) 240, 244
CviJI RGCY 3 cut(s) 56, 73, 228
CviKI_1 RGCY 3 cut(s) 56, 73, 228
CviQI GTAC 1 cut(s) 237
DdeI CTNAG 1 cut(s) 96
DpnI GATC 1 cut(s) 125
DpnII GATC 1 cut(s) 123
EaeI YGGCCR 1 cut(s) 54
EcoT22I ATGCAT 1 cut(s) 245
FaeI CATG 2 cut(s) 243, 247
FaiI YATR 2 cut(s) 241, 245
FatI CATG 2 cut(s) 239, 243
Fnu4HI GCNGC 1 cut(s) 30
Fsp4HI GCNGC 1 cut(s) 30
GluI GCNGC 1 cut(s) 30
HaeIII GGCC 2 cut(s) 56, 73
HgaI GACGC 1 cut(s) 5
Hin1II CATG 2 cut(s) 243, 247
HinfI GANTC 2 cut(s) 98, 133
HphI GGTGA 1 cut(s) 199
Hpy166II GTNNAC 2 cut(s) 182, 249
Hpy188I TCNGA 1 cut(s) 138
Hpy8I GTNNAC 2 cut(s) 182, 249
HpyAV CCTTC 1 cut(s) 43
HpyCH4V TGCA 3 cut(s) 192, 243, 249
HpyF10VI GCNNNNNNNGC 1 cut(s) 13
HpyF3I CTNAG 1 cut(s) 96
Hsp92II CATG 2 cut(s) 243, 247
Kzo9I GATC 1 cut(s) 123
LmnI GCTCC 2 cut(s) 4, 199
LpnPI CCDG 1 cut(s) 223
Lsp1109I GCAGC 1 cut(s) 16
MalI GATC 1 cut(s) 125
MboI GATC 1 cut(s) 123
MfeI CAATTG 1 cut(s) 175
MflI RGATCY 1 cut(s) 123
MhlI GDGCHC 2 cut(s) 143, 251
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 2 cut(s) 175, 203
MluNI TGGCCA 1 cut(s) 56
MlyI GAGTC 1 cut(s) 107
MnlI CCTC 4 cut(s) 79, 109, 178, 206
Mox20I TGGCCA 1 cut(s) 56
Mph1103I ATGCAT 1 cut(s) 245
MscI TGGCCA 1 cut(s) 56
MseI TTAA 1 cut(s) 262
MslI CAYNNNNRTG 1 cut(s) 244
Msp20I TGGCCA 1 cut(s) 56
MunI CAATTG 1 cut(s) 175
MwoI GCNNNNNNNGC 1 cut(s) 13
NdeII GATC 1 cut(s) 123
NlaIII CATG 2 cut(s) 243, 247
NlaIV GGNNCC 1 cut(s) 72
NsiI ATGCAT 1 cut(s) 245
NspI RCATGY 2 cut(s) 243, 247
PfeI GAWTC 1 cut(s) 133
PflFI GACNNNGTC 1 cut(s) 97
PkrI GCNGC 1 cut(s) 31
PleI GAGTC 1 cut(s) 106
PpsI GAGTC 1 cut(s) 106
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 71
PsuI RGATCY 1 cut(s) 123
PsyI GACNNNGTC 1 cut(s) 97
RsaI GTAC 1 cut(s) 238
RsaNI GTAC 1 cut(s) 237
RseI CAYNNNNRTG 1 cut(s) 244
SaqAI TTAA 1 cut(s) 262
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 1 cut(s) 123
Sau96I GGNCC 1 cut(s) 71
SchI GAGTC 1 cut(s) 107
SduI GDGCHC 2 cut(s) 143, 251
SetI ASST 2 cut(s) 111, 146
SmiMI CAYNNNNRTG 1 cut(s) 244
Sse9I AATT 2 cut(s) 175, 203
TasI AATT 2 cut(s) 175, 203
TfiI GAWTC 1 cut(s) 133
Tru1I TTAA 1 cut(s) 262
Tru9I TTAA 1 cut(s) 262
TseI GCWGC 1 cut(s) 29
TspDTI ATGAA 1 cut(s) 25
Tth111I GACNNNGTC 1 cut(s) 97
VneI GTGCAC 1 cut(s) 247
XceI RCATGY 2 cut(s) 243, 247
XcmI CCANNNNNNNNNTGG 1 cut(s) 64
Zsp2I ATGCAT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.