MD02G1207600.v1.1

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001299
Physical Location & Seq
Reverse (-)
20952036 .. 20952648
613 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1207600.v1.1.491

Sequence Viewer

Length: 264 bp
ATGGAGCGTTCAATGCGTCTTGTTTCAATCGCTTTCGTTTTTGTCCTGCTTTTGGCCGCTACCGAGATGGGGCCAATGGGTGTTAAGGCAAGGACTGAGTCAAGCAAGGCAGTTGAGGGAAAGATCTGTGAGTTTCCGAGCACATTGTTCAAGGGGTTGTGCTTTTCGTCTAACAATTGTAAACACACCTGCAGAAAGGAGCAATTCACCAGAGGCAAATGTAGTCTCCTCACCCGTACATGCATGTGCACAAAAAAGTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

88

Amino Acids

9.71

Weight (kDa)

9.46

Isoelectric Point (pI)

29.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 41 - 87 1.4e-13 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 197
Acc36I ACCTGC 1 cut(s) 197
AciI CCGC 1 cut(s) 57
AcoI YGGCCR 1 cut(s) 54
AfaI GTAC 1 cut(s) 238
AfiI CCNNNNNNNGG 2 cut(s) 52, 69
AgsI TTSAA 3 cut(s) 12, 27, 151
Alw21I GWGCWC 2 cut(s) 143, 251
Alw26I GTCTC 1 cut(s) 230
Alw44I GTGCAC 1 cut(s) 247
AoxI GGCC 2 cut(s) 54, 71
ApaLI GTGCAC 1 cut(s) 247
AspS9I GGNCC 1 cut(s) 71
AsuHPI GGTGA 2 cut(s) 199, 223
BaeGI GKGCMC 1 cut(s) 251
Bbv12I GWGCWC 2 cut(s) 143, 251
BccI CCATC 1 cut(s) 61
BcoDI GTCTC 1 cut(s) 230
BfmI CTRYAG 1 cut(s) 190
BfuAI ACCTGC 1 cut(s) 197
BglII AGATCT 1 cut(s) 123
BisI GCNGC 1 cut(s) 57
BlsI GCNGC 1 cut(s) 58
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 1 cut(s) 72
Bsc4I CCNNNNNNNGG 2 cut(s) 52, 69
BseLI CCNNNNNNNGG 2 cut(s) 52, 69
BseMII CTCAG 1 cut(s) 87
BseRI GAGGAG 1 cut(s) 218
BseSI GKGCMC 1 cut(s) 251
BshFI GGCC 2 cut(s) 56, 73
BsiHKAI GWGCWC 2 cut(s) 143, 251
BslI CCNNNNNNNGG 2 cut(s) 52, 69
BsmAI GTCTC 1 cut(s) 230
BsnI GGCC 2 cut(s) 56, 73
Bsp1286I GDGCHC 2 cut(s) 143, 251
Bsp143I GATC 1 cut(s) 123
BspACI CCGC 1 cut(s) 57
BspANI GGCC 2 cut(s) 56, 73
BspCNI CTCAG 1 cut(s) 88
BspLI GGNNCC 1 cut(s) 72
BspMAI CTGCAG 1 cut(s) 194
BspMI ACCTGC 1 cut(s) 197
BssMI GATC 1 cut(s) 123
BstDEI CTNAG 1 cut(s) 96
BstKTI GATC 1 cut(s) 126
BstMAI GTCTC 1 cut(s) 230
BstMBI GATC 1 cut(s) 123
BstMWI GCNNNNNNNGC 1 cut(s) 13
BstNSI RCATGY 2 cut(s) 243, 247
BstSFI CTRYAG 1 cut(s) 190
BstSLI GKGCMC 1 cut(s) 251
BstX2I RGATCY 1 cut(s) 123
BstYI RGATCY 1 cut(s) 123
BsuRI GGCC 2 cut(s) 56, 73
BveI ACCTGC 1 cut(s) 197
Cfr13I GGNCC 1 cut(s) 71
CseI GACGC 1 cut(s) 5
Csp6I GTAC 1 cut(s) 237
CviAII CATG 2 cut(s) 240, 244
CviJI RGCY 2 cut(s) 56, 73
CviKI_1 RGCY 2 cut(s) 56, 73
CviQI GTAC 1 cut(s) 237
DdeI CTNAG 1 cut(s) 96
DpnI GATC 1 cut(s) 125
DpnII GATC 1 cut(s) 123
EaeI YGGCCR 1 cut(s) 54
EcoT22I ATGCAT 1 cut(s) 245
FaeI CATG 2 cut(s) 243, 247
FaiI YATR 2 cut(s) 241, 245
FatI CATG 2 cut(s) 239, 243
Fnu4HI GCNGC 1 cut(s) 57
Fsp4HI GCNGC 1 cut(s) 57
GluI GCNGC 1 cut(s) 57
HaeIII GGCC 2 cut(s) 56, 73
HgaI GACGC 1 cut(s) 5
Hin1II CATG 2 cut(s) 243, 247
HinfI GANTC 1 cut(s) 98
HphI GGTGA 2 cut(s) 199, 223
Hpy166II GTNNAC 2 cut(s) 182, 249
Hpy188I TCNGA 1 cut(s) 138
Hpy8I GTNNAC 2 cut(s) 182, 249
HpyCH4V TGCA 3 cut(s) 192, 243, 249
HpyF10VI GCNNNNNNNGC 1 cut(s) 13
HpyF3I CTNAG 1 cut(s) 96
Hsp92II CATG 2 cut(s) 243, 247
Kzo9I GATC 1 cut(s) 123
LmnI GCTCC 2 cut(s) 4, 199
LpnPI CCDG 3 cut(s) 59, 202, 223
MalI GATC 1 cut(s) 125
MboI GATC 1 cut(s) 123
MfeI CAATTG 1 cut(s) 175
MflI RGATCY 1 cut(s) 123
MhlI GDGCHC 2 cut(s) 143, 251
MluCI AATT 2 cut(s) 175, 203
MlyI GAGTC 1 cut(s) 107
MnlI CCTC 3 cut(s) 109, 206, 239
Mph1103I ATGCAT 1 cut(s) 245
MseI TTAA 1 cut(s) 84
MslI CAYNNNNRTG 1 cut(s) 244
MunI CAATTG 1 cut(s) 175
MwoI GCNNNNNNNGC 1 cut(s) 13
NdeII GATC 1 cut(s) 123
NlaIII CATG 2 cut(s) 243, 247
NlaIV GGNNCC 1 cut(s) 72
NsiI ATGCAT 1 cut(s) 245
NspI RCATGY 2 cut(s) 243, 247
PaqCI CACCTGC 1 cut(s) 197
PflFI GACNNNGTC 1 cut(s) 97
PkrI GCNGC 1 cut(s) 58
PleI GAGTC 1 cut(s) 106
PpsI GAGTC 1 cut(s) 106
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 71
PstI CTGCAG 1 cut(s) 194
PsuI RGATCY 1 cut(s) 123
PsyI GACNNNGTC 1 cut(s) 97
RsaI GTAC 1 cut(s) 238
RsaNI GTAC 1 cut(s) 237
RseI CAYNNNNRTG 1 cut(s) 244
SaqAI TTAA 1 cut(s) 84
SatI GCNGC 1 cut(s) 57
Sau3AI GATC 1 cut(s) 123
Sau96I GGNCC 1 cut(s) 71
SchI GAGTC 1 cut(s) 107
SduI GDGCHC 2 cut(s) 143, 251
SetI ASST 1 cut(s) 191
SfcI CTRYAG 1 cut(s) 190
SmiMI CAYNNNNRTG 1 cut(s) 244
Sse9I AATT 2 cut(s) 175, 203
SsiI CCGC 1 cut(s) 57
TasI AATT 2 cut(s) 175, 203
TauI GCSGC 1 cut(s) 59
Tru1I TTAA 1 cut(s) 84
Tru9I TTAA 1 cut(s) 84
Tth111I GACNNNGTC 1 cut(s) 97
VneI GTGCAC 1 cut(s) 247
XceI RCATGY 2 cut(s) 243, 247
Zsp2I ATGCAT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.