MD14G1062000.v1.1

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001299
Physical Location & Seq
Reverse (-)
6576371 .. 6577036
666 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1062000.v1.1.491

Sequence Viewer

Length: 264 bp
ATGAAGTGTTCCGGACGTTTGTGTTCAACTGTCTTCATCCTCGTCGTCCTTCTGGTGGCTATTGGGTTAGCTGAGGGAAAGATAAGACATATGTCGAAGGATGAGAATTCGTTGACTTGTGAGTCTCGGAGCCATAAATACCACGGTCCTTGCTTTAGGTTTATTTTTTCAAGCAATTGTGCAGTTACATGCAAAGGAGAGGGATTTACAGGAGGCAAATGTAAGGGCTTCCATATGAGATGTATATGCACTAAACAATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009505 GO:0016020 GO:0030312 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

88

Amino Acids

9.6

Weight (kDa)

9.15

Isoelectric Point (pI)

31.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 39 - 87 5.1e-14 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 121
AccIII TCCGGA 1 cut(s) 11
AcsI RAATTY 1 cut(s) 106
AfiI CCNNNNNNNGG 1 cut(s) 55
AgsI TTSAA 2 cut(s) 27, 171
AluBI AGCT 1 cut(s) 71
AluI AGCT 1 cut(s) 71
Alw26I GTCTC 1 cut(s) 129
Aor13HI TCCGGA 1 cut(s) 11
ApoI RAATTY 1 cut(s) 106
AspS9I GGNCC 1 cut(s) 146
AvaII GGWCC 1 cut(s) 146
BbsI GAAGAC 1 cut(s) 25
BbvCI CCTCAGC 1 cut(s) 72
BcoDI GTCTC 1 cut(s) 129
Bme18I GGWCC 1 cut(s) 146
BmgT120I GGNCC 1 cut(s) 146
BmiI GGNNCC 1 cut(s) 131
BoxI GACNNNNGTC 1 cut(s) 91
BpiI GAAGAC 1 cut(s) 25
Bpu10I CCTNAGC 1 cut(s) 72
BsaJI CCNNGG 1 cut(s) 142
BsaWI WCCGGW 1 cut(s) 11
Bsc4I CCNNNNNNNGG 1 cut(s) 55
BseAI TCCGGA 1 cut(s) 11
BseDI CCNNGG 1 cut(s) 142
BseGI GGATG 2 cut(s) 36, 106
BseLI CCNNNNNNNGG 1 cut(s) 55
BseMII CTCAG 1 cut(s) 63
BsgI GTGCAG 1 cut(s) 201
BsiSI CCGG 1 cut(s) 12
BslI CCNNNNNNNGG 1 cut(s) 55
BsmAI GTCTC 1 cut(s) 129
Bsp13I TCCGGA 1 cut(s) 11
BspCNI CTCAG 1 cut(s) 64
BspEI TCCGGA 1 cut(s) 11
BspLI GGNNCC 1 cut(s) 131
BssECI CCNNGG 1 cut(s) 142
Bst4CI ACNGT 2 cut(s) 31, 146
BstDEI CTNAG 1 cut(s) 72
BstDSI CCRYGG 1 cut(s) 142
BstF5I GGATG 2 cut(s) 36, 106
BstMAI GTCTC 1 cut(s) 129
BstNSI RCATGY 1 cut(s) 192
BstPAI GACNNNNGTC 1 cut(s) 91
BstV2I GAAGAC 1 cut(s) 25
BtgI CCRYGG 1 cut(s) 142
BtsCI GGATG 2 cut(s) 36, 106
Cfr13I GGNCC 1 cut(s) 146
CspCI CAANNNNNGTGG 2 cut(s) 131, 166
CviAII CATG 1 cut(s) 189
CviJI RGCY 4 cut(s) 59, 71, 132, 228
CviKI_1 RGCY 4 cut(s) 59, 71, 132, 228
DdeI CTNAG 1 cut(s) 72
DrdI GACNNNNNNGTC 1 cut(s) 121
DseDI GACNNNNNNGTC 1 cut(s) 121
Eco47I GGWCC 1 cut(s) 146
EcoRI GAATTC 1 cut(s) 106
FaeI CATG 1 cut(s) 192
FaiI YATR 8 cut(s) 90, 92, 135, 190, 234, 236, 245, 247
FatI CATG 1 cut(s) 188
FauNDI CATATG 2 cut(s) 90, 234
FokI GGATG 2 cut(s) 23, 113
HapII CCGG 1 cut(s) 12
Hin1II CATG 1 cut(s) 192
HincII GTYRAC 1 cut(s) 114
HindII GTYRAC 1 cut(s) 114
HinfI GANTC 1 cut(s) 122
HpaII CCGG 1 cut(s) 12
Hpy166II GTNNAC 1 cut(s) 114
Hpy188I TCNGA 1 cut(s) 129
Hpy188III TCNNGA 1 cut(s) 12
Hpy8I GTNNAC 1 cut(s) 114
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 2 cut(s) 59, 91
HpyCH4III ACNGT 2 cut(s) 31, 146
HpyCH4IV ACGT 1 cut(s) 16
HpyCH4V TGCA 3 cut(s) 182, 192, 249
HpyF3I CTNAG 1 cut(s) 72
HpySE526I ACGT 1 cut(s) 16
Hsp92II CATG 1 cut(s) 192
Kpn2I TCCGGA 1 cut(s) 11
LmnI GCTCC 1 cut(s) 129
LpnPI CCDG 3 cut(s) 25, 38, 195
MaeII ACGT 1 cut(s) 16
MaeIII GTNAC 1 cut(s) 184
MboII GAAGA 1 cut(s) 25
MfeI CAATTG 1 cut(s) 175
MluCI AATT 2 cut(s) 106, 175
MlyI GAGTC 1 cut(s) 131
MnlI CCTC 4 cut(s) 50, 67, 193, 206
MroI TCCGGA 1 cut(s) 11
MseI TTAA 1 cut(s) 262
MspI CCGG 1 cut(s) 12
MunI CAATTG 1 cut(s) 175
NdeI CATATG 2 cut(s) 90, 234
NlaIII CATG 1 cut(s) 192
NlaIV GGNNCC 1 cut(s) 131
NspI RCATGY 1 cut(s) 192
PleI GAGTC 1 cut(s) 130
PpsI GAGTC 1 cut(s) 130
PshAI GACNNNNGTC 1 cut(s) 91
PspN4I GGNNCC 1 cut(s) 131
PspPI GGNCC 1 cut(s) 146
SaqAI TTAA 1 cut(s) 262
Sau96I GGNCC 1 cut(s) 146
SchI GAGTC 1 cut(s) 131
SetI ASST 3 cut(s) 19, 73, 161
SinI GGWCC 1 cut(s) 146
Sse9I AATT 2 cut(s) 106, 175
TaaI ACNGT 2 cut(s) 31, 146
TaiI ACGT 1 cut(s) 19
TaqI TCGA 1 cut(s) 95
TasI AATT 2 cut(s) 106, 175
Tru1I TTAA 1 cut(s) 262
Tru9I TTAA 1 cut(s) 262
TspDTI ATGAA 2 cut(s) 17, 25
VpaK11BI GGWCC 1 cut(s) 146
XapI RAATTY 1 cut(s) 106
XceI RCATGY 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.