pycom13g00990

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001299
Physical Location & Seq
Reverse (-)
633771 .. 634117
347 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g00990.1

Sequence Viewer

Length: 264 bp
ATGGAGCGTTCAATGCCTCTTGTTTCAGCCGTCTTCGTTCTTGTCTTGCTTTTGGCTGCTACCGAGATGGGGCCAATGAGTGTTGAGGCAAGGACTGAGTCAAGCAAGGCAGTTGAGGGAAAGATTTGCGAGGTTCCGAGCACCTTGTTCAAGGGGTTGTGCTTTTCCTCTAACAACTGTAAACACACCTGCAGAAAAGAGCAATTCACCAGAGGCAAATGTAGTGGTTTGACCCGTACATGCATGTGCACCAAAAAGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

88

Amino Acids

9.53

Weight (kDa)

9.04

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 41 - 87 2.3e-14 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 197
Acc36I ACCTGC 1 cut(s) 197
AfaI GTAC 1 cut(s) 238
AfiI CCNNNNNNNGG 1 cut(s) 69
AgsI TTSAA 2 cut(s) 12, 151
Alw21I GWGCWC 2 cut(s) 143, 251
Alw44I GTGCAC 1 cut(s) 247
AoxI GGCC 1 cut(s) 71
ApaLI GTGCAC 1 cut(s) 247
ApeKI GCWGC 1 cut(s) 56
AspS9I GGNCC 1 cut(s) 71
AsuHPI GGTGA 1 cut(s) 199
BaeGI GKGCMC 1 cut(s) 251
BbsI GAAGAC 1 cut(s) 25
Bbv12I GWGCWC 2 cut(s) 143, 251
BbvI GCAGC 1 cut(s) 43
BccI CCATC 1 cut(s) 61
BceAI ACGGC 1 cut(s) 14
BfmI CTRYAG 1 cut(s) 190
BfuAI ACCTGC 1 cut(s) 197
BisI GCNGC 1 cut(s) 57
BlsI GCNGC 1 cut(s) 58
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 2 cut(s) 72, 135
BpiI GAAGAC 1 cut(s) 25
Bsc4I CCNNNNNNNGG 1 cut(s) 69
BseLI CCNNNNNNNGG 1 cut(s) 69
BseMII CTCAG 1 cut(s) 87
BseSI GKGCMC 1 cut(s) 251
BseXI GCAGC 1 cut(s) 43
BshFI GGCC 1 cut(s) 73
BsiHKAI GWGCWC 2 cut(s) 143, 251
BslI CCNNNNNNNGG 1 cut(s) 69
BsnI GGCC 1 cut(s) 73
Bsp1286I GDGCHC 2 cut(s) 143, 251
BspANI GGCC 1 cut(s) 73
BspCNI CTCAG 1 cut(s) 88
BspLI GGNNCC 2 cut(s) 72, 135
BspMAI CTGCAG 1 cut(s) 194
BspMI ACCTGC 1 cut(s) 197
Bst4CI ACNGT 1 cut(s) 179
BstDEI CTNAG 1 cut(s) 96
BstMWI GCNNNNNNNGC 1 cut(s) 13
BstNSI RCATGY 2 cut(s) 243, 247
BstSFI CTRYAG 1 cut(s) 190
BstSLI GKGCMC 1 cut(s) 251
BstV1I GCAGC 1 cut(s) 43
BstV2I GAAGAC 1 cut(s) 25
BsuRI GGCC 1 cut(s) 73
BveI ACCTGC 1 cut(s) 197
Cfr13I GGNCC 1 cut(s) 71
Csp6I GTAC 1 cut(s) 237
CspCI CAANNNNNGTGG 2 cut(s) 205, 240
CviAII CATG 2 cut(s) 240, 244
CviJI RGCY 3 cut(s) 29, 56, 73
CviKI_1 RGCY 3 cut(s) 29, 56, 73
CviQI GTAC 1 cut(s) 237
DdeI CTNAG 1 cut(s) 96
EcoT22I ATGCAT 1 cut(s) 245
FaeI CATG 2 cut(s) 243, 247
FaiI YATR 2 cut(s) 241, 245
FatI CATG 2 cut(s) 239, 243
Fnu4HI GCNGC 1 cut(s) 57
Fsp4HI GCNGC 1 cut(s) 57
GluI GCNGC 1 cut(s) 57
HaeIII GGCC 1 cut(s) 73
Hin1II CATG 2 cut(s) 243, 247
HinfI GANTC 1 cut(s) 98
HphI GGTGA 1 cut(s) 199
Hpy166II GTNNAC 2 cut(s) 182, 249
Hpy188I TCNGA 1 cut(s) 138
Hpy8I GTNNAC 2 cut(s) 182, 249
HpyCH4III ACNGT 1 cut(s) 179
HpyCH4V TGCA 3 cut(s) 192, 243, 249
HpyF10VI GCNNNNNNNGC 1 cut(s) 13
HpyF3I CTNAG 1 cut(s) 96
Hsp92II CATG 2 cut(s) 243, 247
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 2 cut(s) 202, 223
Lsp1109I GCAGC 1 cut(s) 43
MboII GAAGA 1 cut(s) 25
MhlI GDGCHC 2 cut(s) 143, 251
MluCI AATT 1 cut(s) 203
MlyI GAGTC 1 cut(s) 107
MnlI CCTC 6 cut(s) 27, 79, 109, 124, 178, 206
Mph1103I ATGCAT 1 cut(s) 245
MseI TTAA 1 cut(s) 262
MslI CAYNNNNRTG 1 cut(s) 244
MwoI GCNNNNNNNGC 1 cut(s) 13
NlaIII CATG 2 cut(s) 243, 247
NlaIV GGNNCC 2 cut(s) 72, 135
NsiI ATGCAT 1 cut(s) 245
NspI RCATGY 2 cut(s) 243, 247
PaqCI CACCTGC 1 cut(s) 197
PflFI GACNNNGTC 1 cut(s) 97
PkrI GCNGC 1 cut(s) 58
PleI GAGTC 1 cut(s) 106
PpsI GAGTC 1 cut(s) 106
PspN4I GGNNCC 2 cut(s) 72, 135
PspPI GGNCC 1 cut(s) 71
PstI CTGCAG 1 cut(s) 194
PsyI GACNNNGTC 1 cut(s) 97
RsaI GTAC 1 cut(s) 238
RsaNI GTAC 1 cut(s) 237
RseI CAYNNNNRTG 1 cut(s) 244
SaqAI TTAA 1 cut(s) 262
SatI GCNGC 1 cut(s) 57
Sau96I GGNCC 1 cut(s) 71
SchI GAGTC 1 cut(s) 107
SduI GDGCHC 2 cut(s) 143, 251
SetI ASST 3 cut(s) 135, 146, 191
SfcI CTRYAG 1 cut(s) 190
SmiMI CAYNNNNRTG 1 cut(s) 244
Sse9I AATT 1 cut(s) 203
TaaI ACNGT 1 cut(s) 179
TasI AATT 1 cut(s) 203
Tru1I TTAA 1 cut(s) 262
Tru9I TTAA 1 cut(s) 262
TseI GCWGC 1 cut(s) 56
Tth111I GACNNNGTC 1 cut(s) 97
VneI GTGCAC 1 cut(s) 247
XceI RCATGY 2 cut(s) 243, 247
Zsp2I ATGCAT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.