Rroxscaffold_6G00401520

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001299
Physical Location & Seq
Forward (+)
23880925 .. 23881295
371 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00401520.1

Sequence Viewer

Length: 261 bp
ATGGCAAATTCCATGCGTTTATTTTCATCCACCTTCATACCCATCTTGCTTCTGGTTGCTATAGAGATGGCGCCACGCATCGCCGAGGGTAAGAGTACAGCTGCCGATATTAAACCGAGGACATGCGAGTCTTTGAGCCACAAATTCAAGGGAATGTGCATCAGCTCAAGCAACTGCGCAACAATGTGTAAAAGTGAGGGCTTCTTAGGAGGTAAATGCCAAGGCTTTAGGATGAGATGTTATTGCACCAAGCGGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009505 GO:0016020 GO:0030312 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

86

Amino Acids

9.45

Weight (kDa)

9.35

Isoelectric Point (pI)

34.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 40 - 86 2.7e-17 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 127
Acc16I TGCGCA 1 cut(s) 178
AccB1I GGYRCC 1 cut(s) 70
AciI CCGC 1 cut(s) 253
AcsI RAATTY 2 cut(s) 7, 143
AcyI GRCGYC 1 cut(s) 71
AfaI GTAC 1 cut(s) 97
AgsI TTSAA 1 cut(s) 148
AluBI AGCT 2 cut(s) 101, 165
AluI AGCT 2 cut(s) 101, 165
ApeKI GCWGC 1 cut(s) 101
ApoI RAATTY 2 cut(s) 7, 143
AspLEI GCGC 2 cut(s) 73, 179
BanI GGYRCC 1 cut(s) 70
BbvI GCAGC 1 cut(s) 88
BccI CCATC 2 cut(s) 50, 61
BfmI CTRYAG 1 cut(s) 60
BfoI RGCGCY 1 cut(s) 74
BisI GCNGC 1 cut(s) 102
BlsI GCNGC 1 cut(s) 103
BmiI GGNNCC 1 cut(s) 72
BmsI GCATC 2 cut(s) 87, 168
BpuEI CTTGAG 1 cut(s) 151
BsaHI GRCGYC 1 cut(s) 71
BsaJI CCNNGG 3 cut(s) 84, 116, 220
BseDI CCNNGG 3 cut(s) 84, 116, 220
BseGI GGATG 2 cut(s) 26, 237
BseXI GCAGC 1 cut(s) 88
BshNI GGYRCC 1 cut(s) 70
BspACI CCGC 1 cut(s) 253
BspLI GGNNCC 1 cut(s) 72
BspT107I GGYRCC 1 cut(s) 70
BssECI CCNNGG 3 cut(s) 84, 116, 220
BssNI GRCGYC 1 cut(s) 71
BssT1I CCWWGG 1 cut(s) 220
BstACI GRCGYC 1 cut(s) 71
BstDEI CTNAG 1 cut(s) 205
BstF5I GGATG 2 cut(s) 26, 237
BstH2I RGCGCY 1 cut(s) 74
BstHHI GCGC 2 cut(s) 73, 179
BstNSI RCATGY 1 cut(s) 126
BstSFI CTRYAG 1 cut(s) 60
BstV1I GCAGC 1 cut(s) 88
BtgZI GCGATG 1 cut(s) 64
BtsCI GGATG 2 cut(s) 26, 237
CfoI GCGC 2 cut(s) 73, 179
Csp6I GTAC 1 cut(s) 96
CviAII CATG 2 cut(s) 13, 123
CviJI RGCY 5 cut(s) 101, 138, 165, 201, 225
CviKI_1 RGCY 5 cut(s) 101, 138, 165, 201, 225
CviQI GTAC 1 cut(s) 96
DdeI CTNAG 1 cut(s) 205
DinI GGCGCC 1 cut(s) 72
DrdI GACNNNNNNGTC 1 cut(s) 127
DseDI GACNNNNNNGTC 1 cut(s) 127
Eco130I CCWWGG 1 cut(s) 220
EcoT14I CCWWGG 1 cut(s) 220
EgeI GGCGCC 1 cut(s) 72
EheI GGCGCC 1 cut(s) 72
ErhI CCWWGG 1 cut(s) 220
FaeI CATG 2 cut(s) 16, 126
FaiI YATR 4 cut(s) 14, 38, 62, 124
FatI CATG 2 cut(s) 12, 122
Fnu4HI GCNGC 1 cut(s) 102
FokI GGATG 2 cut(s) 13, 244
Fsp4HI GCNGC 1 cut(s) 102
FspI TGCGCA 1 cut(s) 178
GlaI GCGC 2 cut(s) 72, 178
GluI GCNGC 1 cut(s) 102
HaeII RGCGCY 1 cut(s) 74
HhaI GCGC 2 cut(s) 73, 179
Hin1I GRCGYC 1 cut(s) 71
Hin1II CATG 2 cut(s) 16, 126
Hin6I GCGC 2 cut(s) 71, 177
HinP1I GCGC 2 cut(s) 71, 177
HinfI GANTC 1 cut(s) 128
HpyAV CCTTC 1 cut(s) 43
HpyCH4V TGCA 2 cut(s) 159, 246
HpyF3I CTNAG 1 cut(s) 205
Hsp92I GRCGYC 1 cut(s) 71
Hsp92II CATG 2 cut(s) 16, 126
HspAI GCGC 2 cut(s) 71, 177
KasI GGCGCC 1 cut(s) 70
LpnPI CCDG 1 cut(s) 38
Lsp1109I GCAGC 1 cut(s) 88
LweI GCATC 2 cut(s) 87, 168
MluCI AATT 2 cut(s) 7, 143
Mly113I GGCGCC 1 cut(s) 71
MlyI GAGTC 1 cut(s) 137
MnlI CCTC 4 cut(s) 79, 111, 190, 203
MseI TTAA 2 cut(s) 111, 259
MspA1I CMGCKG 1 cut(s) 101
NarI GGCGCC 1 cut(s) 71
NlaIII CATG 2 cut(s) 16, 126
NlaIV GGNNCC 1 cut(s) 72
NmeAIII GCCGAG 1 cut(s) 109
NsbI TGCGCA 1 cut(s) 178
NspI RCATGY 1 cut(s) 126
PkrI GCNGC 1 cut(s) 103
PleI GAGTC 1 cut(s) 136
PluTI GGCGCC 1 cut(s) 74
PpsI GAGTC 1 cut(s) 136
PspN4I GGNNCC 1 cut(s) 72
PvuII CAGCTG 1 cut(s) 101
RsaI GTAC 1 cut(s) 97
RsaNI GTAC 1 cut(s) 96
SaqAI TTAA 2 cut(s) 111, 259
SatI GCNGC 1 cut(s) 102
SchI GAGTC 1 cut(s) 137
SetI ASST 4 cut(s) 35, 103, 167, 214
SfaNI GCATC 2 cut(s) 87, 168
SfcI CTRYAG 1 cut(s) 60
SfoI GGCGCC 1 cut(s) 72
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
Sse9I AATT 2 cut(s) 7, 143
SsiI CCGC 1 cut(s) 253
SspDI GGCGCC 1 cut(s) 70
StyI CCWWGG 1 cut(s) 220
TasI AATT 2 cut(s) 7, 143
TatI WGTACW 1 cut(s) 95
Tru1I TTAA 2 cut(s) 111, 259
Tru9I TTAA 2 cut(s) 111, 259
TseI GCWGC 1 cut(s) 101
TspDTI ATGAA 2 cut(s) 15, 25
XapI RAATTY 2 cut(s) 7, 143
XceI RCATGY 1 cut(s) 126
XcmI CCANNNNNNNNNTGG 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.