Rw2G012500

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001299
Physical Location & Seq
Forward (+)
13061387 .. 13061958
572 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G012500.1

Sequence Viewer

Length: 237 bp
ATGGACCGCTCCATGCGTTTGTTTTCAGCTGCTCTCATCCTAGCCATTCTTGTAGTGGCTACAGAGATTGGACAAATAGCTGTTGAGGCAGGTAGGACATGTCAGTCTCCGAGCCATAAGTTCTCCGGAATGTGCTTCTTCTCCGGCAATTGCGCAAAAATTTGCAAACAAGAAGGCTTCCTCGGAGGCGATTGCGGCGGCTTCCGCCGTAGATGCTTTTGCAACAAAGAGTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

78

Amino Acids

8.47

Weight (kDa)

8.73

Isoelectric Point (pI)

68.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 32 - 78 2.3e-15 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 103
Acc16I TGCGCA 1 cut(s) 154
Acc36I ACCTGC 1 cut(s) 80
AccBSI CCGCTC 1 cut(s) 9
AccIII TCCGGA 1 cut(s) 125
AciI CCGC 4 cut(s) 7, 195, 198, 205
AcsI RAATTY 1 cut(s) 159
AflIII ACRYGT 1 cut(s) 98
AluBI AGCT 2 cut(s) 29, 80
AluI AGCT 2 cut(s) 29, 80
Alw26I GTCTC 1 cut(s) 111
Aor13HI TCCGGA 1 cut(s) 125
ApeKI GCWGC 1 cut(s) 29
ApoI RAATTY 1 cut(s) 159
AspLEI GCGC 1 cut(s) 155
AspS9I GGNCC 1 cut(s) 4
AvaII GGWCC 1 cut(s) 4
BbvI GCAGC 1 cut(s) 16
BceAI ACGGC 1 cut(s) 192
BcoDI GTCTC 1 cut(s) 111
BfaI CTAG 1 cut(s) 41
BfmI CTRYAG 1 cut(s) 60
BfuAI ACCTGC 1 cut(s) 80
BisI GCNGC 3 cut(s) 30, 196, 199
BlsI GCNGC 3 cut(s) 31, 197, 200
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 1 cut(s) 4
BmsI GCATC 1 cut(s) 203
BsaJI CCNNGG 1 cut(s) 181
BsaWI WCCGGW 1 cut(s) 125
BseAI TCCGGA 1 cut(s) 125
BseDI CCNNGG 1 cut(s) 181
BseGI GGATG 1 cut(s) 36
BseXI GCAGC 1 cut(s) 16
BsiSI CCGG 2 cut(s) 126, 144
BsmAI GTCTC 1 cut(s) 111
Bsp13I TCCGGA 1 cut(s) 125
BspACI CCGC 4 cut(s) 7, 195, 198, 205
BspEI TCCGGA 1 cut(s) 125
BspMI ACCTGC 1 cut(s) 80
BsrBI CCGCTC 1 cut(s) 9
BssECI CCNNGG 1 cut(s) 181
BstF5I GGATG 1 cut(s) 36
BstHHI GCGC 1 cut(s) 155
BstMAI GTCTC 1 cut(s) 111
BstMWI GCNNNNNNNGC 4 cut(s) 86, 195, 204, 213
BstNSI RCATGY 1 cut(s) 102
BstSFI CTRYAG 1 cut(s) 60
BstV1I GCAGC 1 cut(s) 16
BtsCI GGATG 1 cut(s) 36
BveI ACCTGC 1 cut(s) 80
CfoI GCGC 1 cut(s) 155
Cfr13I GGNCC 1 cut(s) 4
CviAII CATG 2 cut(s) 13, 99
CviJI RGCY 7 cut(s) 29, 44, 59, 80, 114, 177, 201
CviKI_1 RGCY 7 cut(s) 29, 44, 59, 80, 114, 177, 201
DrdI GACNNNNNNGTC 1 cut(s) 103
DseDI GACNNNNNNGTC 1 cut(s) 103
EciI GGCGGA 1 cut(s) 194
Eco47I GGWCC 1 cut(s) 4
FaeI CATG 2 cut(s) 16, 102
FaiI YATR 3 cut(s) 14, 100, 117
FatI CATG 2 cut(s) 12, 98
Fnu4HI GCNGC 3 cut(s) 30, 196, 199
FokI GGATG 1 cut(s) 23
Fsp4HI GCNGC 3 cut(s) 30, 196, 199
FspBI CTAG 1 cut(s) 41
FspI TGCGCA 1 cut(s) 154
GlaI GCGC 1 cut(s) 154
GluI GCNGC 3 cut(s) 30, 196, 199
HapII CCGG 2 cut(s) 126, 144
HhaI GCGC 1 cut(s) 155
Hin1II CATG 2 cut(s) 16, 102
Hin6I GCGC 1 cut(s) 153
HinP1I GCGC 1 cut(s) 153
HpaII CCGG 2 cut(s) 126, 144
Hpy188I TCNGA 2 cut(s) 111, 185
Hpy188III TCNNGA 1 cut(s) 126
HpyAV CCTTC 1 cut(s) 167
HpyCH4V TGCA 2 cut(s) 165, 222
HpyF10VI GCNNNNNNNGC 4 cut(s) 86, 195, 204, 213
Hsp92II CATG 2 cut(s) 16, 102
HspAI GCGC 1 cut(s) 153
Kpn2I TCCGGA 1 cut(s) 125
LmnI GCTCC 1 cut(s) 14
LpnPI CCDG 3 cut(s) 75, 139, 157
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 1 cut(s) 203
MaeI CTAG 1 cut(s) 41
MbiI CCGCTC 1 cut(s) 9
MboII GAAGA 1 cut(s) 130
MfeI CAATTG 1 cut(s) 148
MluCI AATT 2 cut(s) 148, 159
MnlI CCTC 3 cut(s) 79, 179, 191
MroI TCCGGA 1 cut(s) 125
MspA1I CMGCKG 1 cut(s) 29
MspI CCGG 2 cut(s) 126, 144
MunI CAATTG 1 cut(s) 148
MwoI GCNNNNNNNGC 4 cut(s) 86, 195, 204, 213
NlaIII CATG 2 cut(s) 16, 102
NsbI TGCGCA 1 cut(s) 154
NspI RCATGY 1 cut(s) 102
PciI ACATGT 1 cut(s) 98
PkrI GCNGC 3 cut(s) 31, 197, 200
PscI ACATGT 1 cut(s) 98
PspPI GGNCC 1 cut(s) 4
PvuII CAGCTG 1 cut(s) 29
SatI GCNGC 3 cut(s) 30, 196, 199
Sau96I GGNCC 1 cut(s) 4
SetI ASST 3 cut(s) 31, 82, 94
SfaNI GCATC 1 cut(s) 203
SfcI CTRYAG 1 cut(s) 60
SinI GGWCC 1 cut(s) 4
Sse9I AATT 2 cut(s) 148, 159
SsiI CCGC 4 cut(s) 7, 195, 198, 205
SspMI CTAG 1 cut(s) 41
TasI AATT 2 cut(s) 148, 159
TauI GCSGC 2 cut(s) 198, 201
TseI GCWGC 1 cut(s) 29
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 1 cut(s) 159
XceI RCATGY 1 cut(s) 102
XcmI CCANNNNNNNNNTGG 1 cut(s) 52
XspI CTAG 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.