MD13G1011400.v1.1

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001299
Physical Location & Seq
Reverse (-)
687336 .. 687944
609 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1011400.v1.1.491

Sequence Viewer

Length: 264 bp
ATGGAGCGTTCCACGCGTCTTGTTTCAGCTGCCTTCGTTCTTGTCCTGATTTTAGCCGCTACCGAGATGGGGCCATTGGGTGTTGAGGCAAGGAGTAAGTCAAGCAAGGAAGTTCAGGAAAGGACCTGCGAGGCTGCGAGCGGCAAGTTCAAGGGGTTGTGCTTTTCATCCACTAACTGTAAAAACACCTGCAAAAGGGAGCAGTTCACTGGAGGCAAATGTCGTGGCTTCCGCCGTAGATGCATGTGCAACAAAAAGTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

88

Amino Acids

9.62

Weight (kDa)

9.74

Isoelectric Point (pI)

47.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 41 - 87 6.3e-16 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 197
Acc36I ACCTGC 2 cut(s) 134, 197
AccBSI CCGCTC 1 cut(s) 141
AccII CGCG 1 cut(s) 16
AciI CCGC 3 cut(s) 57, 141, 232
AfiI CCNNNNNNNGG 2 cut(s) 69, 195
AflIII ACRYGT 1 cut(s) 14
AgsI TTSAA 1 cut(s) 151
AluBI AGCT 1 cut(s) 29
AluI AGCT 1 cut(s) 29
AoxI GGCC 1 cut(s) 71
ApeKI GCWGC 2 cut(s) 29, 134
AspS9I GGNCC 2 cut(s) 71, 123
AvaII GGWCC 1 cut(s) 123
BbvI GCAGC 2 cut(s) 16, 121
BccI CCATC 1 cut(s) 61
BceAI ACGGC 1 cut(s) 219
BfuAI ACCTGC 2 cut(s) 134, 197
BisI GCNGC 4 cut(s) 30, 57, 135, 142
BlsI GCNGC 4 cut(s) 31, 58, 136, 143
Bme18I GGWCC 1 cut(s) 123
BmgT120I GGNCC 2 cut(s) 71, 123
BmiI GGNNCC 1 cut(s) 72
BmsI GCATC 1 cut(s) 230
BpmI CTGGAG 1 cut(s) 231
Bsc4I CCNNNNNNNGG 2 cut(s) 69, 195
Bse1I ACTGG 1 cut(s) 214
BseGI GGATG 1 cut(s) 167
BseLI CCNNNNNNNGG 2 cut(s) 69, 195
BseNI ACTGG 1 cut(s) 214
BseXI GCAGC 2 cut(s) 16, 121
Bsh1236I CGCG 1 cut(s) 16
BshFI GGCC 1 cut(s) 73
BslI CCNNNNNNNGG 2 cut(s) 69, 195
BsnI GGCC 1 cut(s) 73
BspACI CCGC 3 cut(s) 57, 141, 232
BspANI GGCC 1 cut(s) 73
BspFNI CGCG 1 cut(s) 16
BspLI GGNNCC 1 cut(s) 72
BspMI ACCTGC 2 cut(s) 134, 197
BsrBI CCGCTC 1 cut(s) 141
BsrI ACTGG 1 cut(s) 214
Bst4CI ACNGT 1 cut(s) 179
BstC8I GCNNGC 1 cut(s) 139
BstENI CCTNNNNNAGG 1 cut(s) 193
BstF5I GGATG 1 cut(s) 167
BstFNI CGCG 1 cut(s) 16
BstMWI GCNNNNNNNGC 2 cut(s) 13, 240
BstNSI RCATGY 1 cut(s) 247
BstUI CGCG 1 cut(s) 16
BstV1I GCAGC 2 cut(s) 16, 121
BsuRI GGCC 1 cut(s) 73
BtsCI GGATG 1 cut(s) 167
BtsIMutI CAGTG 1 cut(s) 207
BveI ACCTGC 2 cut(s) 134, 197
Cac8I GCNNGC 1 cut(s) 139
Cfr13I GGNCC 2 cut(s) 71, 123
CseI GACGC 1 cut(s) 5
CspCI CAANNNNNGTGG 3 cut(s) 36, 205, 240
CviAII CATG 1 cut(s) 244
CviJI RGCY 5 cut(s) 29, 56, 73, 134, 228
CviKI_1 RGCY 5 cut(s) 29, 56, 73, 134, 228
EciI GGCGGA 1 cut(s) 221
Eco47I GGWCC 1 cut(s) 123
EcoNI CCTNNNNNAGG 1 cut(s) 193
EcoO109I RGGNCCY 1 cut(s) 123
EcoT22I ATGCAT 1 cut(s) 245
FaeI CATG 1 cut(s) 247
FaiI YATR 1 cut(s) 245
FatI CATG 1 cut(s) 243
Fnu4HI GCNGC 4 cut(s) 30, 57, 135, 142
FokI GGATG 1 cut(s) 154
Fsp4HI GCNGC 4 cut(s) 30, 57, 135, 142
GluI GCNGC 4 cut(s) 30, 57, 135, 142
GsuI CTGGAG 1 cut(s) 231
HaeIII GGCC 1 cut(s) 73
HgaI GACGC 1 cut(s) 5
Hin1II CATG 1 cut(s) 247
Hpy166II GTNNAC 1 cut(s) 207
Hpy188III TCNNGA 2 cut(s) 46, 116
Hpy8I GTNNAC 1 cut(s) 207
HpyAV CCTTC 1 cut(s) 43
HpyCH4III ACNGT 1 cut(s) 179
HpyCH4V TGCA 3 cut(s) 192, 243, 249
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 240
Hsp92II CATG 1 cut(s) 247
LmnI GCTCC 2 cut(s) 4, 199
LpnPI CCDG 5 cut(s) 59, 101, 139, 195, 202
Lsp1109I GCAGC 2 cut(s) 16, 121
LweI GCATC 1 cut(s) 230
MbiI CCGCTC 1 cut(s) 141
MluI ACGCGT 1 cut(s) 14
MnlI CCTC 3 cut(s) 79, 124, 206
Mph1103I ATGCAT 1 cut(s) 245
MspA1I CMGCKG 1 cut(s) 29
MvnI CGCG 1 cut(s) 16
MwoI GCNNNNNNNGC 2 cut(s) 13, 240
NlaIII CATG 1 cut(s) 247
NlaIV GGNNCC 1 cut(s) 72
NsiI ATGCAT 1 cut(s) 245
NspI RCATGY 1 cut(s) 247
PaqCI CACCTGC 1 cut(s) 197
PkrI GCNGC 4 cut(s) 31, 58, 136, 143
PpuMI RGGWCCY 1 cut(s) 123
Psp5II RGGWCCY 1 cut(s) 123
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 2 cut(s) 71, 123
PspPPI RGGWCCY 1 cut(s) 123
PvuII CAGCTG 1 cut(s) 29
SatI GCNGC 4 cut(s) 30, 57, 135, 142
Sau96I GGNCC 2 cut(s) 71, 123
SetI ASST 3 cut(s) 31, 128, 191
SfaNI GCATC 1 cut(s) 230
SinI GGWCC 1 cut(s) 123
SsiI CCGC 3 cut(s) 57, 141, 232
TaaI ACNGT 1 cut(s) 179
TauI GCSGC 2 cut(s) 59, 144
TscAI CASTG 1 cut(s) 214
TseI GCWGC 2 cut(s) 29, 134
TspDTI ATGAA 1 cut(s) 156
TspRI CASTG 1 cut(s) 214
VpaK11BI GGWCC 1 cut(s) 123
XagI CCTNNNNNAGG 1 cut(s) 193
XceI RCATGY 1 cut(s) 247
Zsp2I ATGCAT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.