MD11G1113600.v1.1

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001299
Physical Location & Seq
Forward (+)
10270020 .. 10270567
548 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1113600.v1.1.491

Sequence Viewer

Length: 264 bp
ATGGAGCATTCAATGCGTCTTGTTTCAGCCGCCTTCGTTCTTGTCTTGCTTTTGGTTGCTACCGATATGGGGCCAATGGGTGTTGAGGCAAGGACTGAGTCAAACAAGGCAGTTGAAGGGAAGATCTGCGAGTATCCGAGCACCCTGTTCAAAGGGTTGTGCTTTTCGTCTAACAACTGTAAACACACCTGCAGAAAGGAGCAATTCACTAGAGGCCATTGTAGTGTCTTCACCCGTGCTTGCGTGTGCACCAAAAAGTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

88

Amino Acids

9.64

Weight (kDa)

8.88

Isoelectric Point (pI)

22.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 41 - 87 3.1e-14 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 197
Acc36I ACCTGC 1 cut(s) 197
AciI CCGC 1 cut(s) 30
AfiI CCNNNNNNNGG 1 cut(s) 69
AgsI TTSAA 3 cut(s) 12, 116, 151
Alw21I GWGCWC 2 cut(s) 143, 251
Alw44I GTGCAC 1 cut(s) 247
AoxI GGCC 2 cut(s) 71, 214
ApaLI GTGCAC 1 cut(s) 247
AspS9I GGNCC 1 cut(s) 71
AsuHPI GGTGA 1 cut(s) 223
BaeGI GKGCMC 1 cut(s) 251
BbsI GAAGAC 1 cut(s) 220
Bbv12I GWGCWC 2 cut(s) 143, 251
BciVI GTATCC 1 cut(s) 144
BfaI CTAG 1 cut(s) 210
BfmI CTRYAG 1 cut(s) 190
BfuAI ACCTGC 1 cut(s) 197
BfuI GTATCC 1 cut(s) 144
BglII AGATCT 1 cut(s) 123
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 1 cut(s) 72
BpiI GAAGAC 1 cut(s) 220
Bsc4I CCNNNNNNNGG 1 cut(s) 69
BseLI CCNNNNNNNGG 1 cut(s) 69
BseMII CTCAG 1 cut(s) 87
BseSI GKGCMC 1 cut(s) 251
BshFI GGCC 2 cut(s) 73, 216
BsiHKAI GWGCWC 2 cut(s) 143, 251
BslI CCNNNNNNNGG 1 cut(s) 69
BsmI GAATGC 1 cut(s) 7
BsnI GGCC 2 cut(s) 73, 216
Bsp1286I GDGCHC 2 cut(s) 143, 251
Bsp143I GATC 1 cut(s) 123
BspACI CCGC 1 cut(s) 30
BspANI GGCC 2 cut(s) 73, 216
BspCNI CTCAG 1 cut(s) 88
BspLI GGNNCC 1 cut(s) 72
BspMAI CTGCAG 1 cut(s) 194
BspMI ACCTGC 1 cut(s) 197
BssMI GATC 1 cut(s) 123
Bst4CI ACNGT 1 cut(s) 179
BstAPI GCANNNNNTGC 1 cut(s) 13
BstC8I GCNNGC 1 cut(s) 241
BstDEI CTNAG 1 cut(s) 96
BstKTI GATC 1 cut(s) 126
BstMBI GATC 1 cut(s) 123
BstMWI GCNNNNNNNGC 1 cut(s) 13
BstSFI CTRYAG 1 cut(s) 190
BstSLI GKGCMC 1 cut(s) 251
BstV2I GAAGAC 1 cut(s) 220
BstX2I RGATCY 1 cut(s) 123
BstYI RGATCY 1 cut(s) 123
BsuI GTATCC 1 cut(s) 144
BsuRI GGCC 2 cut(s) 73, 216
BveI ACCTGC 1 cut(s) 197
Cac8I GCNNGC 1 cut(s) 241
Cfr13I GGNCC 1 cut(s) 71
CseI GACGC 1 cut(s) 5
CviJI RGCY 3 cut(s) 29, 73, 216
CviKI_1 RGCY 3 cut(s) 29, 73, 216
DdeI CTNAG 1 cut(s) 96
DpnI GATC 1 cut(s) 125
DpnII GATC 1 cut(s) 123
FaiI YATR 1 cut(s) 68
Fnu4HI GCNGC 1 cut(s) 30
Fsp4HI GCNGC 1 cut(s) 30
FspBI CTAG 1 cut(s) 210
GluI GCNGC 1 cut(s) 30
HaeIII GGCC 2 cut(s) 73, 216
HgaI GACGC 1 cut(s) 5
HinfI GANTC 1 cut(s) 98
HphI GGTGA 1 cut(s) 223
Hpy166II GTNNAC 2 cut(s) 182, 249
Hpy188I TCNGA 1 cut(s) 138
Hpy8I GTNNAC 2 cut(s) 182, 249
HpyAV CCTTC 2 cut(s) 43, 110
HpyCH4III ACNGT 1 cut(s) 179
HpyCH4V TGCA 2 cut(s) 192, 249
HpyF10VI GCNNNNNNNGC 1 cut(s) 13
HpyF3I CTNAG 1 cut(s) 96
Kzo9I GATC 1 cut(s) 123
LmnI GCTCC 2 cut(s) 4, 199
LpnPI CCDG 2 cut(s) 158, 202
MaeI CTAG 1 cut(s) 210
MalI GATC 1 cut(s) 125
MboI GATC 1 cut(s) 123
MboII GAAGA 2 cut(s) 133, 220
MflI RGATCY 1 cut(s) 123
MhlI GDGCHC 2 cut(s) 143, 251
MluCI AATT 1 cut(s) 203
MlyI GAGTC 1 cut(s) 107
MnlI CCTC 2 cut(s) 79, 206
MslI CAYNNNNRTG 1 cut(s) 222
Mva1269I GAATGC 1 cut(s) 7
MwoI GCNNNNNNNGC 1 cut(s) 13
NdeII GATC 1 cut(s) 123
NlaIV GGNNCC 1 cut(s) 72
PaqCI CACCTGC 1 cut(s) 197
PctI GAATGC 1 cut(s) 7
PflFI GACNNNGTC 1 cut(s) 97
PkrI GCNGC 1 cut(s) 31
PleI GAGTC 1 cut(s) 106
PpsI GAGTC 1 cut(s) 106
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 71
PstI CTGCAG 1 cut(s) 194
PsuI RGATCY 1 cut(s) 123
PsyI GACNNNGTC 1 cut(s) 97
RseI CAYNNNNRTG 1 cut(s) 222
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 1 cut(s) 123
Sau96I GGNCC 1 cut(s) 71
SchI GAGTC 1 cut(s) 107
SduI GDGCHC 2 cut(s) 143, 251
SetI ASST 1 cut(s) 191
SfcI CTRYAG 1 cut(s) 190
SmiMI CAYNNNNRTG 1 cut(s) 222
Sse9I AATT 1 cut(s) 203
SsiI CCGC 1 cut(s) 30
SspMI CTAG 1 cut(s) 210
TaaI ACNGT 1 cut(s) 179
TasI AATT 1 cut(s) 203
TauI GCSGC 1 cut(s) 32
Tth111I GACNNNGTC 1 cut(s) 97
VneI GTGCAC 1 cut(s) 247
XspI CTAG 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.