Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0016111

Physical Location & Seq

Genomic Coordinates Forward (+)

37202452 .. 37204494

Gene Structure 2043 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1334500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2043 bp

ATGGCATTCAAAAACGTTGTTATCGAACAGAAAAATAAGAAAGCAGAAGACGATTCAGACGACCAAGGGGCCAAGGGGCCAAGGGGCCAAGGCAATGATTTAATGGTCCTCCTTTCAAAACCGTCAAAGAATATTGGGGGTGTGTTTGAGTGGAACTCCGCCATAAGAGAATGTGTGAAGCAAAATTGTTCCCACAAAGCCCTCCTCCTCTTTCGCCAAATGAAACAAAATGGTCTGGAAGCTGACAACTTCACCTTTCCCTTGCTTGCCAAAGCGTCTGCCAATCTGTGCAGCCTCAGATCCTCCCAAATCATTCACTCCAATGTCCTCAAGTCGCCGTTCCGGTCCGATCTCTATGTGCAAACAGCAATGCTTGACATGTACGTCAAGTGCCAACAACTCACTGATGCCTGCCTTCTGTTCGACAGAATTCCCGTGAGAGACGTGGCTTCCTGGAACGTGATGGTTCTCGCTTTTGCGCAATCCGGGTTTCTTGATAAAGTGTTGGGTTTGTTCCGTGACATGAGGTTTGACGGAATTCTGCCAGATACTGTTACAGTTGTGGGGTTGACTCGGGCAAGTTTGGATTCGAAAAATTTGGCATTGGTGAAGTCCGTGCACGCGTTTGGGATTCGAATTGGGATAGATGGAGATGTTTCAATGGCCAACACTTGGATTTCTGCCTATGCCAAGTGCGAGGACTTGAGTTCCGCCATGGCTGTATTTAATGGGGTTGAGATTGGTGTGAGGACTGTTGTGTCTTGGAACTCATTAATAGCAGGATATGGAAGCTTAGAAAAGTTTATCAATGCTCTAAGTTTTTACAAATGGATGTTGTGCGATGGATATAGGCCCGATACAAGCACTATCGTAAGCCTCCTTTCTTCGTGCATTCAGCCTGATAAACTGTTCCAGGGCGCGCTGATTCATTGTCATGGAATTAAACTGGGCTGTGATTCTGATATTTTTGTTGTCAATGCCCTTATATCTATGTATTCCAGATGCAGTGATATTTTATCTTCTCGATTTTTATTTGATGGCATGACTGATAGGACTTGTGTTTCATGGACTGCTATGATCAGTGGATATGCTGAGAGAGGGGATTTGGACGAGGCACTAAGGTTGTTTCATGCTATGGAAGTTGCTGGTGTGAAACCCGATTTGGTTACTGTCCTTTCACTGGTTTCAGGCTGCGGCCTAACAGGAGCACTTGAGCTTGGAAAATGGATTCATCAATATGCCTTTTCTAATGGGTTAAGAGACAATATAGTGGTCTGCAATGCATTAATAGACATGCATGCAAAATGTGGAAATATTATTGGTGCTCGAGAGCTCTTCAATGTCTTGCCAGTGAGAACTGTTGTTTCCTGGACAACGATGATCACAGGGTGTGCTTTGAATGGCGAATACAAAGAAGCTCTGGACCTTTTCAGTCTGATGGTGGAGTTTGGATTGAAACCAAACCACTTGACGTTTCTTGCCATTCTTCAAGCTTGCACTCATGCAGGGTTTCTTGAGAAAGGATTGGAGCTCTTTCACATGATGAGAGAAGTTTACAATATAAATCCCGGGGTAGATCATTATTCCTGTATCGCCGATCTTCTTGGACGTAAAGGGAGGTTAAAAGAAGCAGTGGAGCTTGTTGAAAGTATGCCAGTGAAACCAGATGCCGGCATATGGAGTGCGTTGCTTAGTGCTTGCAAGATTCATCGCAACGTAGAGATGGGTGAATACGCTTGTGGTCGTCTATTTGAGTTGGAGCCCGACCTCGAGGGGGCAGTTCCATTTGTGGAGATGGCTAACATATATGCATCGGAGAGAAGGTGGGATGAAGCGGCTGCAGTAAGAAGAGCGATGAAGATTAACAAAGTGAAAAAATTTCCGGGACAAAGCCTTGTTCGTGTAAATGGGAAGCCTCACGTGTTTACAGTCGAAGATCGGGGTCATCCTGACAGCTTACTTTTATATGCAACGTTAGATAGTTTGAGTTTGCAGTCGAAAGAAGAAGAATACTGGCCATATTTAGAGGATTTTGTGACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04330 map04330

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

681

Amino Acids

75.39

Weight (kDa)

6.05

Isoelectric Point (pI)

37.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	48 - 92	2.8e-06	PPR repeat family
PPR_2	PF13041	251 - 297	1.1e-07	PPR repeat family
PPR_2	PF13041	354 - 398	3.7e-11	PPR repeat family
PPR	PF01535	354 - 384	4.8e-08	PPR repeat
PPR_2	PF13041	453 - 500	9.9e-11	PPR repeat family
PPR_3	PF13812	454 - 499	1.4e-06	Pentatricopeptide repeat domain
PPR	PF01535	455 - 485	3.5e-08	PPR repeat
E_motif	PF20431	570 - 633	3.4e-13	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0016111)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19191
fragaria_vesca	FvH4_1g22044
malus_domestica	MD15G1334500.v1.1
prunus_persica	Prupe.1G178100_v2.0.a1
pyrus_communis	pycom15g29090 pycom15g29100
rosa_chinensis	RchiOBHm_Chr2g0116121
rosa_laevigata	RLG00000018241
rosa_multiflora	Rmu_sc0002288.1_g000012
rosa_roxburghii	Rroxscaffold_2G00127480
rosa_rugosa	Rorug02G0203600
rosa_samantha	Rh2BG271000 Rh2CG266300 Rh2DG268200
rosa_wichuraiana	Rw2G020360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	383, 757
AbsI	CCTCGAGG	1 cut(s)	1769
Acc16I	TGCGCA	1 cut(s)	480
AccB7I	CCANNNNNTGG	1 cut(s)	672
AccII	CGCG	2 cut(s)	623, 920
AciI	CCGC	4 cut(s)	159, 711, 1194, 1835
AclI	AACGTT	2 cut(s)	15, 1973
AclWI	GGATC	1 cut(s)	294
AcoI	YGGCCR	2 cut(s)	663, 2015
AcsI	RAATTY	4 cut(s)	429, 537, 595, 1877
AcvI	CACGTG	1 cut(s)	1921
AdeI	CACNNNGTG	1 cut(s)	1389
AfaI	GTAC	1 cut(s)	383
AfiI	CCNNNNNNNGG	5 cut(s)	672, 1180, 1670, 1677, 1774
AflIII	ACRYGT	3 cut(s)	378, 621, 1920
AgsI	TTSAA	8 cut(s)	10, 117, 660, 1339, 1399, 1456, 1490, 1646
AjiI	CACGTC	1 cut(s)	445
AjnI	CCWGG	3 cut(s)	452, 912, 1367
AjuI	GAANNNNNNNTTGG	2 cut(s)	1145, 1177
AluBI	AGCT	9 cut(s)	242, 792, 1216, 1333, 1418, 1493, 1531, 1639, 1956
AluI	AGCT	9 cut(s)	242, 792, 1216, 1333, 1418, 1493, 1531, 1639, 1956
Alw21I	GWGCWC	5 cut(s)	621, 1210, 1327, 1335, 1533
Alw26I	GTCTC	2 cut(s)	435, 1254
Alw44I	GTGCAC	1 cut(s)	617
AlwI	GGATC	1 cut(s)	294
AlwNI	CAGNNNCTG	1 cut(s)	551
Ama87I	CYCGRG	4 cut(s)	573, 1326, 1568, 1769
AoxI	GGCC	7 cut(s)	69, 77, 85, 663, 851, 1195, 2015
ApaLI	GTGCAC	1 cut(s)	617
ApeKI	GCWGC	3 cut(s)	291, 1191, 1838
ApoI	RAATTY	4 cut(s)	429, 537, 595, 1877
ArsI	GACNNNNNNTTYG	2 cut(s)	512, 544
AseI	ATTAAT	2 cut(s)	773, 1286
AspLEI	GCGC	3 cut(s)	481, 920, 922
AspS9I	GGNCC	7 cut(s)	69, 77, 85, 106, 345, 852, 1423
AsuC2I	CCSGG	4 cut(s)	487, 1569, 1570, 1884
AsuHPI	GGTGA	3 cut(s)	244, 619, 1739
AsuII	TTCGAA	2 cut(s)	590, 634
AvaI	CYCGRG	4 cut(s)	573, 1326, 1568, 1769
AvaII	GGWCC	3 cut(s)	106, 345, 1423
BaeGI	GKGCMC	1 cut(s)	621
BalI	TGGCCA	2 cut(s)	665, 2017
BanII	GRGCYC	3 cut(s)	1335, 1533, 1764
BbrPI	CACGTG	1 cut(s)	1921
BbsI	GAAGAC	1 cut(s)	54
Bbv12I	GWGCWC	5 cut(s)	621, 1210, 1327, 1335, 1533
BbvI	GCAGC	3 cut(s)	303, 1178, 1825
BccI	CCATC	7 cut(s)	457, 641, 836, 1031, 1432, 1717, 1789
BceAI	ACGGC	1 cut(s)	322
BciT130I	CCWGG	3 cut(s)	454, 914, 1369
BclI	TGATCA	2 cut(s)	1077, 1380
BcnI	CCSGG	4 cut(s)	487, 1569, 1570, 1884
BcoDI	GTCTC	2 cut(s)	435, 1254
BfmI	CTRYAG	1 cut(s)	1839
BisI	GCNGC	5 cut(s)	292, 1192, 1195, 1836, 1839
BlsI	GCNGC	5 cut(s)	293, 1193, 1196, 1837, 1840
Bme1390I	CCNGG	7 cut(s)	454, 487, 914, 1369, 1569, 1570, 1884
Bme18I	GGWCC	3 cut(s)	106, 345, 1423
BmeT110I	CYCGRG	4 cut(s)	573, 1326, 1568, 1769
BmgBI	CACGTC	1 cut(s)	445
BmgT120I	GGNCC	7 cut(s)	69, 77, 85, 106, 345, 852, 1423
BmiI	GGNNCC	4 cut(s)	70, 78, 86, 1761
BmrFI	CCNGG	7 cut(s)	454, 487, 914, 1369, 1569, 1570, 1884
BmrI	ACTGGG	1 cut(s)	956
BmsI	GCATC	4 cut(s)	397, 992, 1657, 1820
BmuI	ACTGGG	1 cut(s)	956
BpiI	GAAGAC	1 cut(s)	54
BplI	GAGNNNNNCTC	2 cut(s)	140, 172
Bpu14I	TTCGAA	2 cut(s)	590, 634
BpuEI	CTTGAG	4 cut(s)	314, 724, 1232, 1535
BpuMI	CCSGG	4 cut(s)	487, 1569, 1570, 1884
BsaAI	YACGTR	1 cut(s)	1921
BsaJI	CCNNGG	8 cut(s)	64, 72, 80, 88, 714, 913, 1568, 1569
BsaWI	WCCGGW	1 cut(s)	342
BsaXI	ACNNNNNCTCC	2 cut(s)	1808, 1838
Bsc4I	CCNNNNNNNGG	5 cut(s)	672, 1180, 1670, 1677, 1774
Bse118I	RCCGGY	1 cut(s)	1670
Bse1I	ACTGG	5 cut(s)	951, 1185, 1349, 1655, 2018
Bse3DI	GCAATG	3 cut(s)	100, 375, 1285
BseBI	CCWGG	3 cut(s)	454, 914, 1369
BseDI	CCNNGG	8 cut(s)	64, 72, 80, 88, 714, 913, 1568, 1569
BseGI	GGATG	3 cut(s)	837, 1834, 1945
BseLI	CCNNNNNNNGG	5 cut(s)	672, 1180, 1670, 1677, 1774
BseMI	GCAATG	3 cut(s)	100, 375, 1285
BseMII	CTCAG	2 cut(s)	310, 1083
BseNI	ACTGG	5 cut(s)	951, 1185, 1349, 1655, 2018
BsePI	GCGCGC	1 cut(s)	918
BseRI	GAGGAG	2 cut(s)	194, 197
BseSI	GKGCMC	1 cut(s)	621
BseXI	GCAGC	3 cut(s)	303, 1178, 1825
BsgI	GTGCAG	1 cut(s)	310
Bsh1236I	CGCG	2 cut(s)	623, 920
BshFI	GGCC	7 cut(s)	71, 79, 87, 665, 853, 1197, 2017
BsiHKAI	GWGCWC	5 cut(s)	621, 1210, 1327, 1335, 1533
BsiHKCI	CYCGRG	4 cut(s)	573, 1326, 1568, 1769
BsiSI	CCGG	5 cut(s)	343, 486, 1569, 1671, 1883
BslFI	GGGAC	1 cut(s)	1899
BslI	CCNNNNNNNGG	5 cut(s)	672, 1180, 1670, 1677, 1774
BsmAI	GTCTC	2 cut(s)	435, 1254
BsmBI	CGTCTC	1 cut(s)	435
BsmFI	GGGAC	1 cut(s)	1899
BsmI	GAATGC	2 cut(s)	5, 891
BsnI	GGCC	7 cut(s)	71, 79, 87, 665, 853, 1197, 2017
BsoBI	CYCGRG	4 cut(s)	573, 1326, 1568, 1769
Bsp119I	TTCGAA	2 cut(s)	590, 634
Bsp1286I	GDGCHC	6 cut(s)	621, 1210, 1327, 1335, 1533, 1764
Bsp143I	GATC	7 cut(s)	299, 349, 1077, 1380, 1576, 1597, 1936
Bsp19I	CCATGG	1 cut(s)	714
BspACI	CCGC	4 cut(s)	159, 711, 1194, 1835
BspANI	GGCC	7 cut(s)	71, 79, 87, 665, 853, 1197, 2017
BspCNI	CTCAG	2 cut(s)	309, 1084
BspFNI	CGCG	2 cut(s)	623, 920
BspLI	GGNNCC	4 cut(s)	70, 78, 86, 1761
BspMAI	CTGCAG	1 cut(s)	1843
BspPI	GGATC	1 cut(s)	294
BspQI	GCTCTTC	2 cut(s)	1340, 1843
BspT104I	TTCGAA	2 cut(s)	590, 634
BsrDI	GCAATG	3 cut(s)	100, 375, 1285
BsrFI	RCCGGY	1 cut(s)	1670
BsrI	ACTGG	5 cut(s)	951, 1185, 1349, 1655, 2018
BssAI	RCCGGY	1 cut(s)	1670
BssECI	CCNNGG	8 cut(s)	64, 72, 80, 88, 714, 913, 1568, 1569
BssHII	GCGCGC	1 cut(s)	918
BssMI	GATC	7 cut(s)	299, 349, 1077, 1380, 1576, 1597, 1936
BssT1I	CCWWGG	5 cut(s)	64, 72, 80, 88, 714
Bst2UI	CCWGG	3 cut(s)	454, 914, 1369
Bst4CI	ACNGT	8 cut(s)	123, 553, 559, 754, 909, 1171, 1360, 1930
Bst6I	CTCTTC	2 cut(s)	1340, 1843
BstBAI	YACGTR	1 cut(s)	1921
BstBI	TTCGAA	2 cut(s)	590, 634
BstC8I	GCNNGC	8 cut(s)	267, 412, 621, 920, 1299, 1495, 1672, 1699
BstDEI	CTNAG	6 cut(s)	296, 793, 815, 1092, 1118, 1691
BstDSI	CCRYGG	1 cut(s)	714
BstF5I	GGATG	3 cut(s)	837, 1834, 1945
BstFNI	CGCG	2 cut(s)	623, 920
BstHHI	GCGC	3 cut(s)	481, 920, 922
BstKTI	GATC	7 cut(s)	302, 352, 1080, 1383, 1579, 1600, 1939
BstMAI	GTCTC	2 cut(s)	435, 1254
BstMBI	GATC	7 cut(s)	299, 349, 1077, 1380, 1576, 1597, 1936
BstNI	CCWGG	3 cut(s)	454, 914, 1369
BstNSI	RCATGY	3 cut(s)	382, 1297, 1301
BstSCI	CCNGG	7 cut(s)	452, 485, 912, 1367, 1567, 1568, 1882
BstSFI	CTRYAG	1 cut(s)	1839
BstSLI	GKGCMC	1 cut(s)	621
BstUI	CGCG	2 cut(s)	623, 920
BstV1I	GCAGC	3 cut(s)	303, 1178, 1825
BstV2I	GAAGAC	1 cut(s)	54
BstX2I	RGATCY	1 cut(s)	299
BstYI	RGATCY	1 cut(s)	299
BsuRI	GGCC	7 cut(s)	71, 79, 87, 665, 853, 1197, 2017
BtgI	CCRYGG	1 cut(s)	714
BtgZI	GCGATG	3 cut(s)	855, 1694, 1868
BtrI	CACGTC	1 cut(s)	445
BtsCI	GGATG	3 cut(s)	837, 1834, 1945
BtsI	GCAGTG	2 cut(s)	1012, 1638
BtsIMutI	CAGTG	7 cut(s)	402, 1012, 1087, 1178, 1356, 1638, 1662
Cac8I	GCNNGC	8 cut(s)	267, 412, 621, 920, 1299, 1495, 1672, 1699
CaiI	CAGNNNCTG	1 cut(s)	551
CfoI	GCGC	3 cut(s)	481, 920, 922
Cfr10I	RCCGGY	1 cut(s)	1670
Cfr13I	GGNCC	7 cut(s)	69, 77, 85, 106, 345, 852, 1423
Cfr9I	CCCGGG	1 cut(s)	1568
CpoI	CGGWCCG	1 cut(s)	345
CseI	GACGC	1 cut(s)	264
Csp6I	GTAC	1 cut(s)	382
CspI	CGGWCCG	1 cut(s)	345
CviQI	GTAC	1 cut(s)	382
DdeI	CTNAG	6 cut(s)	296, 793, 815, 1092, 1118, 1691
DpnI	GATC	7 cut(s)	301, 351, 1079, 1382, 1578, 1599, 1938
DpnII	GATC	7 cut(s)	299, 349, 1077, 1380, 1576, 1597, 1936
DraIII	CACNNNGTG	1 cut(s)	1389
DrdI	GACNNNNNNGTC	2 cut(s)	383, 757
DseDI	GACNNNNNNGTC	2 cut(s)	383, 757
EaeI	YGGCCR	2 cut(s)	663, 2015
Eam1104I	CTCTTC	2 cut(s)	1340, 1843
EarI	CTCTTC	2 cut(s)	1340, 1843
EciI	GGCGGA	2 cut(s)	148, 700
Ecl136II	GAGCTC	2 cut(s)	1333, 1531
Eco130I	CCWWGG	5 cut(s)	64, 72, 80, 88, 714
Eco24I	GRGCYC	3 cut(s)	1335, 1533, 1764
Eco47I	GGWCC	3 cut(s)	106, 345, 1423
Eco53kI	GAGCTC	2 cut(s)	1333, 1531
Eco72I	CACGTG	1 cut(s)	1921
Eco88I	CYCGRG	4 cut(s)	573, 1326, 1568, 1769
EcoICRI	GAGCTC	2 cut(s)	1333, 1531
EcoRI	GAATTC	2 cut(s)	429, 537
EcoRII	CCWGG	3 cut(s)	452, 912, 1367
EcoT14I	CCWWGG	5 cut(s)	64, 72, 80, 88, 714
EcoT22I	ATGCAT	3 cut(s)	1285, 1299, 1813
EcoT38I	GRGCYC	3 cut(s)	1335, 1533, 1764
ErhI	CCWWGG	5 cut(s)	64, 72, 80, 88, 714
Esp3I	CGTCTC	1 cut(s)	435
FaqI	GGGAC	1 cut(s)	1899
FauNDI	CATATG	1 cut(s)	1676
FbaI	TGATCA	2 cut(s)	1077, 1380
Fnu4HI	GCNGC	5 cut(s)	292, 1192, 1195, 1836, 1839
FokI	GGATG	3 cut(s)	844, 1841, 1932
FriOI	GRGCYC	3 cut(s)	1335, 1533, 1764
Fsp4HI	GCNGC	5 cut(s)	292, 1192, 1195, 1836, 1839
FspI	TGCGCA	1 cut(s)	480
GlaI	GCGC	3 cut(s)	480, 919, 921
GluI	GCNGC	5 cut(s)	292, 1192, 1195, 1836, 1839
HaeIII	GGCC	7 cut(s)	71, 79, 87, 665, 853, 1197, 2017
HapII	CCGG	5 cut(s)	343, 486, 1569, 1671, 1883
HgaI	GACGC	1 cut(s)	264
HhaI	GCGC	3 cut(s)	481, 920, 922
Hin6I	GCGC	3 cut(s)	479, 918, 920
HinP1I	GCGC	3 cut(s)	479, 918, 920
HincII	GTYRAC	1 cut(s)	570
HindII	GTYRAC	1 cut(s)	570
HindIII	AAGCTT	2 cut(s)	790, 1491
HinfI	GANTC	8 cut(s)	53, 571, 587, 631, 925, 956, 1228, 1705
HpaII	CCGG	5 cut(s)	343, 486, 1569, 1671, 1883
HphI	GGTGA	3 cut(s)	244, 619, 1739
Hpy166II	GTNNAC	4 cut(s)	570, 619, 1555, 1926
Hpy188I	TCNGA	6 cut(s)	58, 299, 349, 961, 1437, 1816
Hpy188III	TCNNGA	8 cut(s)	236, 494, 999, 1023, 1328, 1421, 1514, 1949
Hpy8I	GTNNAC	4 cut(s)	570, 619, 1555, 1926
HpyAV	CCTTC	2 cut(s)	425, 1815
HpyCH4III	ACNGT	8 cut(s)	123, 553, 559, 754, 909, 1171, 1360, 1930
HpyCH4IV	ACGT	9 cut(s)	15, 384, 444, 459, 1472, 1609, 1716, 1920, 1973
HpyF3I	CTNAG	6 cut(s)	296, 793, 815, 1092, 1118, 1691
HpySE526I	ACGT	9 cut(s)	15, 384, 444, 459, 1472, 1609, 1716, 1920, 1973
HspAI	GCGC	3 cut(s)	479, 918, 920
KroI	GCCGGC	1 cut(s)	1670
KroNI	GCCGGC	1 cut(s)	1672
Ksp22I	TGATCA	2 cut(s)	1077, 1380
Kzo9I	GATC	7 cut(s)	299, 349, 1077, 1380, 1576, 1597, 1936
LguI	GCTCTTC	2 cut(s)	1340, 1843
LmnI	GCTCC	4 cut(s)	1205, 1528, 1636, 1759
Lsp1109I	GCAGC	3 cut(s)	303, 1178, 1825
LweI	GCATC	4 cut(s)	397, 992, 1657, 1820
MaeII	ACGT	9 cut(s)	15, 384, 444, 459, 1472, 1609, 1716, 1920, 1973
MaeIII	GTNAC	4 cut(s)	518, 553, 1165, 2035
MalI	GATC	7 cut(s)	301, 351, 1079, 1382, 1578, 1599, 1938
MboI	GATC	7 cut(s)	299, 349, 1077, 1380, 1576, 1597, 1936
MflI	RGATCY	1 cut(s)	299
MhlI	GDGCHC	6 cut(s)	621, 1210, 1327, 1335, 1533, 1764
MlsI	TGGCCA	2 cut(s)	665, 2017
MluCI	AATT	7 cut(s)	184, 429, 537, 595, 636, 939, 1877
MluI	ACGCGT	1 cut(s)	621
MluNI	TGGCCA	2 cut(s)	665, 2017
MlyI	GAGTC	1 cut(s)	565
MmeI	TCCRAC	1 cut(s)	1737
Mox20I	TGGCCA	2 cut(s)	665, 2017
Mph1103I	ATGCAT	3 cut(s)	1285, 1299, 1813
MroNI	GCCGGC	1 cut(s)	1670
MscI	TGGCCA	2 cut(s)	665, 2017
MseI	TTAA	8 cut(s)	101, 726, 773, 942, 1256, 1286, 1622, 1863
MslI	CAYNNNNRTG	3 cut(s)	321, 933, 1236
Msp20I	TGGCCA	2 cut(s)	665, 2017
MspI	CCGG	5 cut(s)	343, 486, 1569, 1671, 1883
MspR9I	CCNGG	7 cut(s)	454, 487, 914, 1369, 1569, 1570, 1884
Mva1269I	GAATGC	2 cut(s)	5, 891
MvaI	CCWGG	3 cut(s)	454, 914, 1369
MvnI	CGCG	2 cut(s)	623, 920
NaeI	GCCGGC	1 cut(s)	1672
NciI	CCSGG	4 cut(s)	487, 1569, 1570, 1884
NcoI	CCATGG	1 cut(s)	714
NdeI	CATATG	1 cut(s)	1676
NdeII	GATC	7 cut(s)	299, 349, 1077, 1380, 1576, 1597, 1936
NgoMIV	GCCGGC	1 cut(s)	1670
NlaIV	GGNNCC	4 cut(s)	70, 78, 86, 1761
NmuCI	GTSAC	2 cut(s)	518, 2035
NsbI	TGCGCA	1 cut(s)	480
NsiI	ATGCAT	3 cut(s)	1285, 1299, 1813
NspI	RCATGY	3 cut(s)	382, 1297, 1301
NspV	TTCGAA	2 cut(s)	590, 634
PaeI	GCATGC	1 cut(s)	1301
PaeR7I	CTCGAG	2 cut(s)	1326, 1769
PauI	GCGCGC	1 cut(s)	918
PciI	ACATGT	1 cut(s)	378
PciSI	GCTCTTC	2 cut(s)	1340, 1843
PcsI	WCGNNNNNNNCGW	2 cut(s)	21, 57
PctI	GAATGC	2 cut(s)	5, 891
PdiI	GCCGGC	1 cut(s)	1672
PfeI	GAWTC	7 cut(s)	53, 587, 631, 925, 956, 1228, 1705
PflMI	CCANNNNNTGG	1 cut(s)	672
PfoI	TCCNGGA	3 cut(s)	452, 1367, 1882
PkrI	GCNGC	5 cut(s)	293, 1193, 1196, 1837, 1840
PleI	GAGTC	1 cut(s)	565
PmaCI	CACGTG	1 cut(s)	1921
PmlI	CACGTG	1 cut(s)	1921
PpsI	GAGTC	1 cut(s)	565
Ppu21I	YACGTR	1 cut(s)	1921
PscI	ACATGT	1 cut(s)	378
PshBI	ATTAAT	2 cut(s)	773, 1286
Psp124BI	GAGCTC	2 cut(s)	1335, 1533
Psp1406I	AACGTT	2 cut(s)	15, 1973
Psp6I	CCWGG	3 cut(s)	452, 912, 1367
PspCI	CACGTG	1 cut(s)	1921
PspGI	CCWGG	3 cut(s)	452, 912, 1367
PspN4I	GGNNCC	4 cut(s)	70, 78, 86, 1761
PspPI	GGNCC	7 cut(s)	69, 77, 85, 106, 345, 852, 1423
PspXI	VCTCGAGB	1 cut(s)	1769
PstI	CTGCAG	1 cut(s)	1843
PstNI	CAGNNNCTG	1 cut(s)	551
PsuI	RGATCY	1 cut(s)	299
PteI	GCGCGC	1 cut(s)	918
RsaI	GTAC	1 cut(s)	383
RsaNI	GTAC	1 cut(s)	382
RseI	CAYNNNNRTG	3 cut(s)	321, 933, 1236
Rsr2I	CGGWCCG	1 cut(s)	345
RsrII	CGGWCCG	1 cut(s)	345
SacI	GAGCTC	2 cut(s)	1335, 1533
SapI	GCTCTTC	2 cut(s)	1340, 1843
SaqAI	TTAA	8 cut(s)	101, 726, 773, 942, 1256, 1286, 1622, 1863
SatI	GCNGC	5 cut(s)	292, 1192, 1195, 1836, 1839
Sau3AI	GATC	7 cut(s)	299, 349, 1077, 1380, 1576, 1597, 1936
Sau96I	GGNCC	7 cut(s)	69, 77, 85, 106, 345, 852, 1423
SchI	GAGTC	1 cut(s)	565
ScrFI	CCNGG	7 cut(s)	454, 487, 914, 1369, 1569, 1570, 1884
SduI	GDGCHC	6 cut(s)	621, 1210, 1327, 1335, 1533, 1764
SfaNI	GCATC	4 cut(s)	397, 992, 1657, 1820
SfcI	CTRYAG	1 cut(s)	1839
Sfr274I	CTCGAG	2 cut(s)	1326, 1769
SfuI	TTCGAA	2 cut(s)	590, 634
SinI	GGWCC	3 cut(s)	106, 345, 1423
SlaI	CTCGAG	2 cut(s)	1326, 1769
SmaI	CCCGGG	1 cut(s)	1570
SmiMI	CAYNNNNRTG	3 cut(s)	321, 933, 1236
SmlI	CTYRAG	6 cut(s)	329, 703, 1211, 1326, 1514, 1769
SmoI	CTYRAG	6 cut(s)	329, 703, 1211, 1326, 1514, 1769
SphI	GCATGC	1 cut(s)	1301
Sse9I	AATT	7 cut(s)	184, 429, 537, 595, 636, 939, 1877
SsiI	CCGC	4 cut(s)	159, 711, 1194, 1835
SspI	AATATT	2 cut(s)	133, 1315
SstI	GAGCTC	2 cut(s)	1335, 1533
StyD4I	CCNGG	7 cut(s)	452, 485, 912, 1367, 1567, 1568, 1882
StyI	CCWWGG	5 cut(s)	64, 72, 80, 88, 714
TaaI	ACNGT	8 cut(s)	123, 553, 559, 754, 909, 1171, 1360, 1930
TaiI	ACGT	9 cut(s)	18, 387, 447, 462, 1475, 1612, 1719, 1923, 1976
TaqI	TCGA	9 cut(s)	24, 423, 590, 634, 1024, 1327, 1770, 1932, 1997
TasI	AATT	7 cut(s)	184, 429, 537, 595, 636, 939, 1877
TauI	GCSGC	2 cut(s)	1197, 1838
TfiI	GAWTC	7 cut(s)	53, 587, 631, 925, 956, 1228, 1705
Tru1I	TTAA	8 cut(s)	101, 726, 773, 942, 1256, 1286, 1622, 1863
Tru9I	TTAA	8 cut(s)	101, 726, 773, 942, 1256, 1286, 1622, 1863
TscAI	CASTG	7 cut(s)	409, 1012, 1087, 1185, 1356, 1638, 1662
TseFI	GTSAC	2 cut(s)	518, 2035
TseI	GCWGC	3 cut(s)	291, 1191, 1838
Tsp45I	GTSAC	2 cut(s)	518, 2035
TspDTI	ATGAA	8 cut(s)	236, 917, 1053, 1118, 1220, 1697, 1845, 1871
TspGWI	ACGGA	3 cut(s)	506, 549, 604
TspMI	CCCGGG	1 cut(s)	1568
TspRI	CASTG	7 cut(s)	409, 1012, 1087, 1185, 1356, 1638, 1662
Van91I	CCANNNNNTGG	1 cut(s)	672
VneI	GTGCAC	1 cut(s)	617
VpaK11BI	GGWCC	3 cut(s)	106, 345, 1423
VspI	ATTAAT	2 cut(s)	773, 1286
XapI	RAATTY	4 cut(s)	429, 537, 595, 1877
XceI	RCATGY	3 cut(s)	382, 1297, 1301
XhoI	CTCGAG	2 cut(s)	1326, 1769
XmaI	CCCGGG	1 cut(s)	1568
Zsp2I	ATGCAT	3 cut(s)	1285, 1299, 1813

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.