MD15G1366200.v1.1

Hydroxyproline-rich glycoprotein family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016863
Physical Location & Seq
Reverse (-)
44477188 .. 44478036
849 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1366200.v1.1.491

Sequence Viewer

Length: 849 bp
ATGGCGGAGCGGTCCCCACCTGATCTCCTCGGTAACAAAAACCACTCTCCTCCTTCTGTCCTTCTTGCAATTCCCGAATACCCCCAATTCCCACCTCCAACACTGCCCCCGTCGACGTCCCGACAGTTGGTCATCTCCCAACCCCAAAATCCTTCCGTCGCCACCTTCCGCTCAGGTACCTACGTTGTCCAAGTCCCCAAGGACCAAATCTACCACGTGCCCCCGCCCGAAAACGCCTACATTGTCGAACGCCACCAGGACCCGTCCCGCAAAAGAAAAAGGCCGTTATGTTGCAACCTTTACTGCATTGGCATCTTGGCTTTCATTGTCCTCCTTGTTGTCATGCTCGTAACGATTTTTTCTGTTATGTTCGCCAAGTCCGAAAATCCCAAATTCAGCGTAGAACGTGTTGTTGTACATAGCAAGAGTAATGGACGGCCGGACTACAGCTTGACGTTGAGGGTTCGAAATCCGAATTCGAGAGTGGCAATTGTGTACAATGGTGGTGGTGCTAGCCTTCATTTCAAGCAAAAAACAATAGCTAACGGAAAATACCCATCTCTTACGCAAGGGACGGGCAAATCAAAAGAGGTTACTATCGTCGTCCACGGTGCAAACGTTATTTTGCCTAAAGAGATTAAGAAAAGCTTGGGAGTTAGTGAGAGCAGCAGCAGCACGAAGAAAGTGCAGGTTAGTTTGTCCCTCAACATGAACATTCCGGCGAGGTTGAGAATTGGGACGTTGAAAAGTAGAAGCATGAAATTTCATGCGACATGTGATATAACGGTGGATGAATTGTCGAAACGGACTAAAGTATTGAAGCAGGAATGCGAAACCCAGCGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

283

Amino Acids

31.26

Weight (kDa)

10.04

Isoelectric Point (pI)

49.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 152 - 258 5.6e-08 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016863)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g37770
malus_domestica MD08G1180500.v1.1 MD15G1366200.v1.1
prunus_persica Prupe.1G513900_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0239521
rosa_laevigata RLG00000000748
rosa_multiflora Rmu_sc0003379.1_g000018
rosa_roxburghii Rroxscaffold_3G00220870
rosa_rugosa Rorug07G0328400
rosa_samantha Rh7AG481900 Rh7BG455000 Rh7CG499900 Rh7DG468100
rosa_wichuraiana Rw7G040380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 119
Acc36I ACCTGC 1 cut(s) 679
Acc65I GGTACC 1 cut(s) 176
AccB1I GGYRCC 1 cut(s) 176
AccBSI CCGCTC 2 cut(s) 10, 171
AccI GTMKAC 1 cut(s) 113
AciI CCGC 6 cut(s) 5, 10, 169, 224, 268, 841
AclI AACGTT 1 cut(s) 618
AcoI YGGCCR 1 cut(s) 437
AcsI RAATTY 3 cut(s) 392, 475, 761
AcvI CACGTG 1 cut(s) 217
AcyI GRCGYC 1 cut(s) 116
AfaI GTAC 3 cut(s) 178, 417, 497
AfiI CCNNNNNNNGG 1 cut(s) 127
AflIII ACRYGT 2 cut(s) 406, 773
AgsI TTSAA 3 cut(s) 526, 745, 820
AhdI GACNNNNNGTC 1 cut(s) 128
AjnI CCWGG 1 cut(s) 255
AluBI AGCT 3 cut(s) 450, 542, 648
AluI AGCT 3 cut(s) 450, 542, 648
AoxI GGCC 2 cut(s) 281, 437
ApeKI GCWGC 3 cut(s) 666, 669, 672
ApoI RAATTY 3 cut(s) 392, 475, 761
Asp718I GGTACC 1 cut(s) 176
AspS9I GGNCC 3 cut(s) 12, 202, 259
AsuII TTCGAA 1 cut(s) 466
AsuNHI GCTAGC 1 cut(s) 512
AvaII GGWCC 3 cut(s) 12, 202, 259
BaeGI GKGCMC 1 cut(s) 222
BaeI ACNNNNGTAYC 2 cut(s) 168, 201
BanI GGYRCC 1 cut(s) 176
BbrPI CACGTG 1 cut(s) 217
BbvI GCAGC 3 cut(s) 678, 681, 684
BccI CCATC 1 cut(s) 565
BceAI ACGGC 2 cut(s) 268, 452
BcgI CGANNNNNNTGC 2 cut(s) 667, 701
BciT130I CCWGG 1 cut(s) 257
BfaI CTAG 1 cut(s) 513
BfmI CTRYAG 1 cut(s) 445
BfuAI ACCTGC 1 cut(s) 679
BisI GCNGC 3 cut(s) 667, 670, 673
BlsI GCNGC 3 cut(s) 668, 671, 674
Bme1390I CCNGG 1 cut(s) 257
Bme18I GGWCC 3 cut(s) 12, 202, 259
BmeRI GACNNNNNGTC 1 cut(s) 128
BmgT120I GGNCC 3 cut(s) 12, 202, 259
BmiI GGNNCC 3 cut(s) 14, 178, 261
BmrFI CCNGG 1 cut(s) 257
BmsI GCATC 1 cut(s) 321
BmtI GCTAGC 1 cut(s) 516
Bpu10I CCTNAGC 1 cut(s) 172
Bpu14I TTCGAA 1 cut(s) 466
BsaAI YACGTR 1 cut(s) 217
BsaHI GRCGYC 1 cut(s) 116
BsaJI CCNNGG 3 cut(s) 28, 198, 607
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bsc4I CCNNNNNNNGG 1 cut(s) 127
BseBI CCWGG 1 cut(s) 257
BseDI CCNNGG 3 cut(s) 28, 198, 607
BseGI GGATG 1 cut(s) 796
BseLI CCNNNNNNNGG 1 cut(s) 127
BseMII CTCAG 1 cut(s) 186
BseRI GAGGAG 2 cut(s) 17, 39
BseSI GKGCMC 1 cut(s) 222
BseX3I CGGCCG 1 cut(s) 437
BseXI GCAGC 3 cut(s) 678, 681, 684
BseYI CCCAGC 1 cut(s) 837
BsgI GTGCAG 1 cut(s) 707
Bsh1285I CGRYCG 1 cut(s) 440
BshFI GGCC 2 cut(s) 283, 439
BshNI GGYRCC 1 cut(s) 176
BsiEI CGRYCG 1 cut(s) 440
BsiSI CCGG 2 cut(s) 440, 719
BslFI GGGAC 6 cut(s) 103, 179, 250, 586, 685, 751
BslI CCNNNNNNNGG 1 cut(s) 127
BsmFI GGGAC 6 cut(s) 103, 179, 250, 586, 685, 751
BsmI GAATGC 1 cut(s) 833
BsnI GGCC 2 cut(s) 283, 439
Bsp119I TTCGAA 1 cut(s) 466
Bsp1286I GDGCHC 1 cut(s) 222
Bsp1407I TGTACA 2 cut(s) 415, 495
Bsp143I GATC 1 cut(s) 22
BspACI CCGC 6 cut(s) 5, 10, 169, 224, 268, 841
BspANI GGCC 2 cut(s) 283, 439
BspCNI CTCAG 1 cut(s) 185
BspLI GGNNCC 3 cut(s) 14, 178, 261
BspMI ACCTGC 1 cut(s) 679
BspOI GCTAGC 1 cut(s) 516
BspT104I TTCGAA 1 cut(s) 466
BspT107I GGYRCC 1 cut(s) 176
BsrBI CCGCTC 2 cut(s) 10, 171
BsrGI TGTACA 2 cut(s) 415, 495
BssECI CCNNGG 3 cut(s) 28, 198, 607
BssMI GATC 1 cut(s) 22
BssNI GRCGYC 1 cut(s) 116
BssT1I CCWWGG 1 cut(s) 198
Bst2UI CCWGG 1 cut(s) 257
Bst4CI ACNGT 3 cut(s) 126, 611, 787
BstACI GRCGYC 1 cut(s) 116
BstAUI TGTACA 2 cut(s) 415, 495
BstBAI YACGTR 1 cut(s) 217
BstBI TTCGAA 1 cut(s) 466
BstC8I GCNNGC 1 cut(s) 514
BstDEI CTNAG 1 cut(s) 172
BstDSI CCRYGG 1 cut(s) 607
BstF5I GGATG 1 cut(s) 796
BstKTI GATC 1 cut(s) 25
BstMBI GATC 1 cut(s) 22
BstMCI CGRYCG 1 cut(s) 440
BstMWI GCNNNNNNNGC 1 cut(s) 672
BstNI CCWGG 1 cut(s) 257
BstNSI RCATGY 1 cut(s) 777
BstSCI CCNGG 1 cut(s) 255
BstSFI CTRYAG 1 cut(s) 445
BstSLI GKGCMC 1 cut(s) 222
BstV1I GCAGC 3 cut(s) 678, 681, 684
BstZI CGGCCG 1 cut(s) 437
BsuRI GGCC 2 cut(s) 283, 439
BtgI CCRYGG 1 cut(s) 607
BtsCI GGATG 1 cut(s) 796
BtsI GCAGTG 1 cut(s) 101
BtsIMutI CAGTG 1 cut(s) 101
BveI ACCTGC 1 cut(s) 679
Cac8I GCNNGC 1 cut(s) 514
Cfr13I GGNCC 3 cut(s) 12, 202, 259
Csp6I GTAC 3 cut(s) 177, 416, 496
CspCI CAANNNNNGTGG 2 cut(s) 487, 522
CviAII CATG 5 cut(s) 343, 709, 757, 767, 774
CviJI RGCY 7 cut(s) 283, 320, 439, 450, 516, 542, 648
CviKI_1 RGCY 7 cut(s) 283, 320, 439, 450, 516, 542, 648
CviQI GTAC 3 cut(s) 177, 416, 496
DdeI CTNAG 1 cut(s) 172
DpnI GATC 1 cut(s) 24
DpnII GATC 1 cut(s) 22
DriI GACNNNNNGTC 1 cut(s) 128
EaeI YGGCCR 1 cut(s) 437
EagI CGGCCG 1 cut(s) 437
Eam1105I GACNNNNNGTC 1 cut(s) 128
EciI GGCGGA 1 cut(s) 20
EclXI CGGCCG 1 cut(s) 437
Eco130I CCWWGG 1 cut(s) 198
Eco47I GGWCC 3 cut(s) 12, 202, 259
Eco52I CGGCCG 1 cut(s) 437
Eco72I CACGTG 1 cut(s) 217
EcoO109I RGGNCCY 1 cut(s) 259
EcoRI GAATTC 1 cut(s) 475
EcoRII CCWGG 1 cut(s) 255
EcoT14I CCWWGG 1 cut(s) 198
ErhI CCWWGG 1 cut(s) 198
FaeI CATG 5 cut(s) 346, 712, 760, 770, 777
FaiI YATR 9 cut(s) 289, 344, 368, 420, 710, 758, 768, 775, 782
FalI AAGNNNNNCTT 2 cut(s) 632, 664
FaqI GGGAC 6 cut(s) 103, 179, 250, 586, 685, 751
FatI CATG 5 cut(s) 342, 708, 756, 766, 773
FauI CCCGC 2 cut(s) 231, 275
FblI GTMKAC 1 cut(s) 113
Fnu4HI GCNGC 3 cut(s) 667, 670, 673
FokI GGATG 1 cut(s) 803
Fsp4HI GCNGC 3 cut(s) 667, 670, 673
FspBI CTAG 1 cut(s) 513
GluI GCNGC 3 cut(s) 667, 670, 673
GsaI CCCAGC 1 cut(s) 841
HaeIII GGCC 2 cut(s) 283, 439
HapII CCGG 2 cut(s) 440, 719
Hin1I GRCGYC 1 cut(s) 116
Hin1II CATG 5 cut(s) 346, 712, 760, 770, 777
HincII GTYRAC 1 cut(s) 114
HindII GTYRAC 1 cut(s) 114
HindIII AAGCTT 1 cut(s) 646
HpaII CCGG 2 cut(s) 440, 719
Hpy166II GTNNAC 3 cut(s) 114, 496, 607
Hpy188I TCNGA 2 cut(s) 382, 474
Hpy188III TCNNGA 3 cut(s) 74, 120, 480
Hpy8I GTNNAC 3 cut(s) 114, 496, 607
Hpy99I CGWCG 4 cut(s) 115, 118, 161, 605
HpyAV CCTTC 5 cut(s) 63, 71, 162, 175, 527
HpyCH4III ACNGT 3 cut(s) 126, 611, 787
HpyCH4IV ACGT 7 cut(s) 116, 183, 216, 406, 455, 618, 740
HpyCH4V TGCA 5 cut(s) 68, 294, 306, 614, 688
HpyF10VI GCNNNNNNNGC 1 cut(s) 672
HpyF3I CTNAG 1 cut(s) 172
HpySE526I ACGT 7 cut(s) 116, 183, 216, 406, 455, 618, 740
Hsp92I GRCGYC 1 cut(s) 116
Hsp92II CATG 5 cut(s) 346, 712, 760, 770, 777
KpnI GGTACC 1 cut(s) 180
Kzo9I GATC 1 cut(s) 22
LmnI GCTCC 1 cut(s) 7
LpnPI CCDG 8 cut(s) 33, 159, 242, 269, 453, 674, 732, 809
Lsp1109I GCAGC 3 cut(s) 678, 681, 684
LweI GCATC 1 cut(s) 321
MaeI CTAG 1 cut(s) 513
MaeII ACGT 7 cut(s) 116, 183, 216, 406, 455, 618, 740
MaeIII GTNAC 3 cut(s) 32, 349, 592
MalI GATC 1 cut(s) 24
MbiI CCGCTC 2 cut(s) 10, 171
MboI GATC 1 cut(s) 22
MboII GAAGA 1 cut(s) 691
MfeI CAATTG 1 cut(s) 489
MhlI GDGCHC 1 cut(s) 222
MluCI AATT 8 cut(s) 69, 86, 392, 475, 489, 732, 761, 794
MmeI TCCRAC 1 cut(s) 122
MnlI CCTC 8 cut(s) 38, 60, 105, 341, 453, 583, 713, 717
MseI TTAA 1 cut(s) 639
MspA1I CMGCKG 1 cut(s) 841
MspI CCGG 2 cut(s) 440, 719
MspR9I CCNGG 1 cut(s) 257
MunI CAATTG 1 cut(s) 489
Mva1269I GAATGC 1 cut(s) 833
MvaI CCWGG 1 cut(s) 257
MwoI GCNNNNNNNGC 1 cut(s) 672
NdeII GATC 1 cut(s) 22
NheI GCTAGC 1 cut(s) 512
NlaIII CATG 5 cut(s) 346, 712, 760, 770, 777
NlaIV GGNNCC 3 cut(s) 14, 178, 261
NspI RCATGY 1 cut(s) 777
NspV TTCGAA 1 cut(s) 466
PciI ACATGT 1 cut(s) 773
PcsI WCGNNNNNNNCGW 2 cut(s) 378, 615
PctI GAATGC 1 cut(s) 833
PkrI GCNGC 3 cut(s) 668, 671, 674
PmaCI CACGTG 1 cut(s) 217
PmlI CACGTG 1 cut(s) 217
Ppu21I YACGTR 1 cut(s) 217
PpuMI RGGWCCY 1 cut(s) 259
PscI ACATGT 1 cut(s) 773
Psp1406I AACGTT 1 cut(s) 618
Psp5II RGGWCCY 1 cut(s) 259
Psp6I CCWGG 1 cut(s) 255
PspCI CACGTG 1 cut(s) 217
PspFI CCCAGC 1 cut(s) 837
PspGI CCWGG 1 cut(s) 255
PspN4I GGNNCC 3 cut(s) 14, 178, 261
PspPI GGNCC 3 cut(s) 12, 202, 259
PspPPI RGGWCCY 1 cut(s) 259
RsaI GTAC 3 cut(s) 178, 417, 497
RsaNI GTAC 3 cut(s) 177, 416, 496
SalI GTCGAC 1 cut(s) 112
SaqAI TTAA 1 cut(s) 639
SatI GCNGC 3 cut(s) 667, 670, 673
Sau3AI GATC 1 cut(s) 22
Sau96I GGNCC 3 cut(s) 12, 202, 259
ScrFI CCNGG 1 cut(s) 257
SduI GDGCHC 1 cut(s) 222
SfaNI GCATC 1 cut(s) 321
SfcI CTRYAG 1 cut(s) 445
SfuI TTCGAA 1 cut(s) 466
SgrDI CGTCGACG 1 cut(s) 112
SinI GGWCC 3 cut(s) 12, 202, 259
Sse9I AATT 8 cut(s) 69, 86, 392, 475, 489, 732, 761, 794
SsiI CCGC 6 cut(s) 5, 10, 169, 224, 268, 841
SspMI CTAG 1 cut(s) 513
StyD4I CCNGG 1 cut(s) 255
StyI CCWWGG 1 cut(s) 198
TaaI ACNGT 3 cut(s) 126, 611, 787
TaiI ACGT 7 cut(s) 119, 186, 219, 409, 458, 621, 743
TaqI TCGA 5 cut(s) 113, 246, 466, 479, 800
TasI AATT 8 cut(s) 69, 86, 392, 475, 489, 732, 761, 794
TatI WGTACW 2 cut(s) 415, 495
Tru1I TTAA 1 cut(s) 639
Tru9I TTAA 1 cut(s) 639
TscAI CASTG 1 cut(s) 108
TseI GCWGC 3 cut(s) 666, 669, 672
TspDTI ATGAA 6 cut(s) 313, 509, 725, 755, 773, 807
TspGWI ACGGA 3 cut(s) 145, 561, 820
TspRI CASTG 1 cut(s) 108
VpaK11BI GGWCC 3 cut(s) 12, 202, 259
XapI RAATTY 3 cut(s) 392, 475, 761
XceI RCATGY 1 cut(s) 777
XmiI GTMKAC 1 cut(s) 113
XspI CTAG 1 cut(s) 513
ZraI GACGTC 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.