MD15G1390700.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016862
Physical Location & Seq
Forward (+)
48508608 .. 48511171
2564 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1390700.v1.1.491

Sequence Viewer

Length: 2292 bp
ATGGCTTCTTTGCCCTCAGTTACTCTCAGTGGCACTCTCAAACTAGACCAAGAATTCCGAAAACACCCCACAAATTTTCTTCCTTCCGAAAAGAGCGTAAATGTTTCGTATCCGAAAAGTCAGACAATCACCAATTCAGATCGAAGTTCAGAACTCCCCAAGTCTCTAGACTTTCGAGAAGCGCTTTTGATGATTAGAGAGGGGGATAAGGTTGAGTCATCTCACTACGTTCCTGTCTTGCAAGAATGTATACACAGGAATTCGGCTTCAGAAGCGGAAGTTGTGCATGCCCACATCATAAAGACCGGAACCCACCAAGATTTGTTTGTCACAACATTCCTTGTCAATGTTTATGCAAAATGTAGAATCATGGAAAGTGCACGTAAAGTTTTTGACAATCTGCCTGGGAGGAACGTTGTTTCGTGGACAAGTTTGATAACGGGCTATGTTCACAATTCGCAGCCAGAAGTTGCCATTCGGGTTTTCAAAGAAATGCTGGAAGCCGGGGCTTATCCTACGAATTACACTCTAGGAATTGTTTTGAACGCTTGTTCTTCTTTGCAGTCCTTAAAATTAGGGAAGCAGTTGCATGGCTATATAATCAAATACCAGATCGATTTTGACACTAGTATCGGCAACTGTCTCTGCAGTTTATACTCGAAATGTGGAAGCTTGGAGTCCGGTATTAAAGCGTTCAAGAAAATCGAGGAAAAAAATGTTATCTCGTGGACTGCAACCATATCTGCTTGTAGTGATAACGGTGAAGCTGCAAGGGGTTTGAGATTTTTCGCTGAGATGCTTTCCGAGGGCGTTGGACCTAATGAGTATACCCTAACCAATGTCTTGAGCTTGTGTTGTTTAATGCTGTCTTTGGGTGTGGGGCAACAGGTTCATTCGTTAAGCATAAAACTTGGCTACGAATCGAACCTACCAATAAAAAACTCTATCATGTATTTATACCTCAAATGTGGACAGATTAATGAGGCTCGAAAGTTGTTTAATGGGATGGAAGCTATCAGCTTGGTTACATGGAATGCAATGATTGCAGGCCATGCACAAATTATGGATCTTGCACAGGATGATGTTTCAGCGTACCAAAGCGGAACTGAAGCACTCAACATTTTCTTGAAACTGAACCGTTCAGGCTTGCAACCGGATTTGTTCACCTTCTCGAGTATCTTAGCTATATGTAGTGGTTTAGTGGCCTTAGAACAGGGAGAACAGATTCATGCTCAGACCATTAAGACGGGGTTTCTCGAAGATATGGTAGTAGGCACTGCCCTAGTCAATATGTACAACAAATGTGGAAGTATTGAGAAAGCAAGTAAAGCTTTTGTGGAGATGTCTACGAGAACTTTGATATCGTGGACAACTATGATTGCAGGTTTTGCGCAGCATGGCCGGACTCAGCAAGCACTGCAGCTCTTCGAGGATATGAGAATTGCAGGAGTAAGGCCGAATCAGATTACTTTCGTGAGTGTTCTCTCGGCTTGTAGCCATGCTGGGATGGTCAACGAAGCTCTCGGTTACTTTGAGATGATGAAAAAAGACTATAAGATTAAGCCTGTGCTGGATCATTTTGCCTGCTTGATTGATATGTACGTGAGGAAGGGTCGGTTAGAGGAAGCGATTGATCTGGCAAAGAAAATGGATCCTCAAGCGAACGAGTTTATATGGTCGATCTTGGTTGCAGGGTGTAGAAGACATGGGAATTTAGAATTGGGATTTTATGCTGCTGAAAGGTTGCTCAAGCTAAAACCAAAAGATACCGAGACTTACACATTGTTGTTGAATTTGTACCACTCTGCAGGAAGATTGAAGGACGTTGCTAAGGTGAGAAAGAAGATGAAAGAGGAGAAGCTTGAAAAATTAAGGGACTGGAGCTGGATTAGCATTAGAGACAAGATCTATTCGTTTCAACAGAACGATAATTTACATCCTTACAGCGCAGATACACAAAAATTCGTACAAAGTTTGATTGATCGAGTCAAGAGTCTTGGATATCAGTCACTAGAAATTTTGGAAGTAACCGATGAGGAGCATGAAGAGCAGACTTTGATTCCAACCGCTTATCATAGCGAAAAATTAGCGATTGCTTTTGGATTGTTGAACATGCCAAATGCTGCACCAATTCGGGTCATCAAGAGCATCGTTATGTGCAGAGATTGCCATAGTTTTGTTAAGTTTGTGTCATTACTGACTGGTAGGGAGATGATCATTCGAGACAGCAAGCGCCTCCACAGATTTGTGAACGGAACATGTTCATGTGGGGACTTCGGAGGTGCTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

764

Amino Acids

85.4

Weight (kDa)

8.12

Isoelectric Point (pI)

33.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 137 - 185 5.2e-12 PPR repeat family
PPR PF01535 140 - 169 1.1e-06 PPR repeat
PPR_2 PF13041 238 - 286 8.5e-08 PPR repeat family
PPR_2 PF13041 453 - 499 1.5e-11 PPR repeat family
PPR PF01535 454 - 483 1.1e-06 PPR repeat
PPR_long PF17177 469 - 552 1.3e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 475 - 535 8.2e-08 Pentatricopeptide repeat domain
E_motif PF20431 569 - 630 9e-15 E motif
DYW_deaminase PF14432 676 - 759 3e-27 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016862)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g33990
malus_domestica MD15G1390700.v1.1
prunus_persica Prupe.1G539500_v2.0.a1
pyrus_communis pycom15g34980
rosa_chinensis RchiOBHm_Chr7g0233371
rosa_laevigata RLG00000001274
rosa_multiflora Rmu_sc0008806.1_g000005
rosa_roxburghii Rroxscaffold_3G00227330
rosa_rugosa Rorug07G0280500
rosa_samantha Rh7BG409100 Rh7CG455500
rosa_wichuraiana Rw0G022470 Rw7G035890 Rw7G035930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1392
Acc36I ACCTGC 1 cut(s) 1373
AccI GTMKAC 3 cut(s) 250, 827, 1346
AciI CCGC 3 cut(s) 275, 1101, 2067
AclI AACGTT 1 cut(s) 414
AclWI GGATC 4 cut(s) 1074, 1581, 1646, 1659
AcoI YGGCCR 1 cut(s) 1399
AcsI RAATTY 7 cut(s) 53, 73, 259, 1711, 1792, 1961, 2016
AcuI CTGAAG 2 cut(s) 252, 1128
AfaI GTAC 5 cut(s) 1094, 1295, 1601, 1799, 1968
AfeI AGCGCT 1 cut(s) 183
AflIII ACRYGT 1 cut(s) 2258
AgsI TTSAA 9 cut(s) 487, 544, 697, 1129, 1792, 1819, 1865, 1919, 2110
AhlI ACTAGT 1 cut(s) 626
AjnI CCWGG 1 cut(s) 403
AjuI GAANNNNNNNTTGG 2 cut(s) 1703, 1735
Alw21I GWGCWC 1 cut(s) 382
Alw26I GTCTC 5 cut(s) 168, 647, 1766, 1893, 2217
Alw44I GTGCAC 1 cut(s) 378
AlwI GGATC 4 cut(s) 1074, 1581, 1646, 1659
Ama87I CYCGRG 1 cut(s) 1171
Aor51HI AGCGCT 1 cut(s) 183
AoxI GGCC 4 cut(s) 1048, 1203, 1399, 1454
ApaLI GTGCAC 1 cut(s) 378
ApeKI GCWGC 6 cut(s) 460, 767, 1393, 1420, 1733, 2123
ApoI RAATTY 7 cut(s) 53, 73, 259, 1711, 1792, 1961, 2016
AseI ATTAAT 1 cut(s) 978
Asp700I GAANNNNTTC 2 cut(s) 1224, 2260
AspLEI GCGC 4 cut(s) 184, 1393, 1949, 2235
AspS9I GGNCC 1 cut(s) 815
AsuC2I CCSGG 1 cut(s) 505
AsuHPI GGTGA 4 cut(s) 121, 773, 1156, 1846
AvaI CYCGRG 1 cut(s) 1171
AvaII GGWCC 1 cut(s) 815
BaeGI GKGCMC 1 cut(s) 382
BaeI ACNNNNGTAYC 2 cut(s) 613, 646
BamHI GGATCC 1 cut(s) 1651
BauI CACGAG 1 cut(s) 724
BbsI GAAGAC 1 cut(s) 1708
Bbv12I GWGCWC 1 cut(s) 382
BbvI GCAGC 6 cut(s) 472, 754, 1405, 1432, 1720, 2110
BccI CCATC 2 cut(s) 1000, 1501
BciT130I CCWGG 1 cut(s) 405
BciVI GTATCC 1 cut(s) 120
BclI TGATCA 1 cut(s) 2214
BcnI CCSGG 1 cut(s) 505
BcoDI GTCTC 5 cut(s) 168, 647, 1766, 1893, 2217
BcuI ACTAGT 1 cut(s) 626
BfaI CTAG 6 cut(s) 44, 167, 530, 627, 1283, 2012
BfmI CTRYAG 3 cut(s) 646, 1418, 1806
BfoI RGCGCY 2 cut(s) 185, 2236
BfuAI ACCTGC 1 cut(s) 1373
BfuI GTATCC 1 cut(s) 120
BglII AGATCT 1 cut(s) 1905
BisI GCNGC 6 cut(s) 461, 768, 1394, 1421, 1734, 2124
BlsI GCNGC 6 cut(s) 462, 769, 1395, 1422, 1735, 2125
Bme1390I CCNGG 2 cut(s) 405, 505
Bme18I GGWCC 1 cut(s) 815
BmeT110I CYCGRG 1 cut(s) 1171
BmgT120I GGNCC 1 cut(s) 815
BmiI GGNNCC 2 cut(s) 310, 1653
BmrFI CCNGG 2 cut(s) 405, 505
BmsI GCATC 2 cut(s) 786, 2157
BpiI GAAGAC 1 cut(s) 1708
BpmI CTGGAG 1 cut(s) 1900
Bpu10I CCTNAGC 1 cut(s) 1830
BpuEI CTTGAG 3 cut(s) 865, 1641, 1733
BpuMI CCSGG 1 cut(s) 505
Bsa29I ATCGAT 1 cut(s) 615
BsaAI YACGTR 2 cut(s) 383, 1603
BsaJI CCNNGG 3 cut(s) 404, 504, 804
BsaWI WCCGGW 3 cut(s) 305, 680, 1153
Bse1I ACTGG 2 cut(s) 1883, 2206
Bse3DI GCAATG 1 cut(s) 1044
BseBI CCWGG 1 cut(s) 405
BseCI ATCGAT 1 cut(s) 615
BseDI CCNNGG 3 cut(s) 404, 504, 804
BseGI GGATG 4 cut(s) 1011, 1084, 1512, 1936
BseMI GCAATG 1 cut(s) 1044
BseMII CTCAG 5 cut(s) 30, 40, 783, 1247, 1421
BseNI ACTGG 2 cut(s) 1883, 2206
BseRI GAGGAG 2 cut(s) 1868, 2051
BseSI GKGCMC 1 cut(s) 382
BseXI GCAGC 6 cut(s) 472, 754, 1405, 1432, 1720, 2110
BseYI CCCAGC 1 cut(s) 1502
BsgI GTGCAG 2 cut(s) 2109, 2179
BshFI GGCC 4 cut(s) 1050, 1205, 1401, 1456
BshVI ATCGAT 1 cut(s) 615
BsiHKAI GWGCWC 1 cut(s) 382
BsiHKCI CYCGRG 1 cut(s) 1171
BsiSI CCGG 5 cut(s) 306, 504, 681, 1154, 1402
BslFI GGGAC 2 cut(s) 1889, 2285
BsmAI GTCTC 5 cut(s) 168, 647, 1766, 1893, 2217
BsmFI GGGAC 2 cut(s) 1889, 2285
BsmI GAATGC 1 cut(s) 1039
BsnI GGCC 4 cut(s) 1050, 1205, 1401, 1456
BsoBI CYCGRG 1 cut(s) 1171
Bsp1286I GDGCHC 1 cut(s) 382
Bsp1407I TGTACA 1 cut(s) 1293
BspACI CCGC 3 cut(s) 275, 1101, 2067
BspANI GGCC 4 cut(s) 1050, 1205, 1401, 1456
BspCNI CTCAG 5 cut(s) 29, 39, 784, 1246, 1420
BspDI ATCGAT 1 cut(s) 615
BspLI GGNNCC 2 cut(s) 310, 1653
BspMAI CTGCAG 3 cut(s) 650, 1422, 1810
BspMI ACCTGC 1 cut(s) 1373
BspPI GGATC 4 cut(s) 1074, 1581, 1646, 1659
BspQI GCTCTTC 2 cut(s) 1430, 2040
BsrDI GCAATG 1 cut(s) 1044
BsrGI TGTACA 1 cut(s) 1293
BsrI ACTGG 2 cut(s) 1883, 2206
BssECI CCNNGG 3 cut(s) 404, 504, 804
BssNAI GTATAC 2 cut(s) 251, 828
BssSI CACGAG 1 cut(s) 724
Bst1107I GTATAC 2 cut(s) 251, 828
Bst2BI CACGAG 1 cut(s) 724
Bst2UI CCWGG 1 cut(s) 405
Bst4CI ACNGT 3 cut(s) 641, 761, 1139
Bst6I CTCTTC 2 cut(s) 1430, 2040
BstAPI GCANNNNNTGC 5 cut(s) 1043, 1052, 1388, 1417, 2166
BstAUI TGTACA 1 cut(s) 1293
BstBAI YACGTR 2 cut(s) 383, 1603
BstC8I GCNNGC 6 cut(s) 288, 1048, 1148, 1413, 1585, 2231
BstDEI CTNAG 8 cut(s) 16, 26, 792, 1180, 1207, 1233, 1407, 1830
BstF5I GGATG 4 cut(s) 1011, 1084, 1512, 1936
BstH2I RGCGCY 2 cut(s) 185, 2236
BstHHI GCGC 4 cut(s) 184, 1393, 1949, 2235
BstMAI GTCTC 5 cut(s) 168, 647, 1766, 1893, 2217
BstMWI GCNNNNNNNGC 9 cut(s) 272, 1043, 1052, 1328, 1388, 1417, 1890, 2047, 2166
BstNI CCWGG 1 cut(s) 405
BstNSI RCATGY 3 cut(s) 290, 2116, 2262
BstSCI CCNGG 2 cut(s) 403, 503
BstSFI CTRYAG 3 cut(s) 646, 1418, 1806
BstSLI GKGCMC 1 cut(s) 382
BstV1I GCAGC 6 cut(s) 472, 754, 1405, 1432, 1720, 2110
BstV2I GAAGAC 1 cut(s) 1708
BstX2I RGATCY 3 cut(s) 1066, 1651, 1905
BstYI RGATCY 3 cut(s) 1066, 1651, 1905
BstZ17I GTATAC 2 cut(s) 251, 828
Bsu15I ATCGAT 1 cut(s) 615
BsuI GTATCC 1 cut(s) 120
BsuRI GGCC 4 cut(s) 1050, 1205, 1401, 1456
BsuTUI ATCGAT 1 cut(s) 615
BtsCI GGATG 4 cut(s) 1011, 1084, 1512, 1936
BtsI GCAGTG 2 cut(s) 1275, 1415
BtsIMutI CAGTG 3 cut(s) 34, 1275, 1415
BveI ACCTGC 1 cut(s) 1373
Cac8I GCNNGC 6 cut(s) 288, 1048, 1148, 1413, 1585, 2231
CfoI GCGC 4 cut(s) 184, 1393, 1949, 2235
Cfr13I GGNCC 1 cut(s) 815
ClaI ATCGAT 1 cut(s) 615
Csp6I GTAC 5 cut(s) 1093, 1294, 1600, 1798, 1967
CspCI CAANNNNNGTGG 2 cut(s) 1285, 1320
CviQI GTAC 5 cut(s) 1093, 1294, 1600, 1798, 1967
DdeI CTNAG 8 cut(s) 16, 26, 792, 1180, 1207, 1233, 1407, 1830
EaeI YGGCCR 1 cut(s) 1399
Eam1104I CTCTTC 2 cut(s) 1430, 2040
EarI CTCTTC 2 cut(s) 1430, 2040
Eco32I GATATC 2 cut(s) 1362, 2003
Eco47I GGWCC 1 cut(s) 815
Eco47III AGCGCT 1 cut(s) 183
Eco57I CTGAAG 2 cut(s) 252, 1128
Eco88I CYCGRG 1 cut(s) 1171
EcoRI GAATTC 2 cut(s) 53, 259
EcoRII CCWGG 1 cut(s) 403
EcoRV GATATC 2 cut(s) 1362, 2003
FalI AAGNNNNNCTT 2 cut(s) 1315, 1347
FaqI GGGAC 2 cut(s) 1889, 2285
FbaI TGATCA 1 cut(s) 2214
FblI GTMKAC 3 cut(s) 250, 827, 1346
Fnu4HI GCNGC 6 cut(s) 461, 768, 1394, 1421, 1734, 2124
FokI GGATG 4 cut(s) 1018, 1091, 1519, 1923
Fsp4HI GCNGC 6 cut(s) 461, 768, 1394, 1421, 1734, 2124
FspBI CTAG 6 cut(s) 44, 167, 530, 627, 1283, 2012
FspI TGCGCA 1 cut(s) 1392
GlaI GCGC 4 cut(s) 183, 1392, 1948, 2234
GluI GCNGC 6 cut(s) 461, 768, 1394, 1421, 1734, 2124
GsaI CCCAGC 1 cut(s) 1506
GsuI CTGGAG 1 cut(s) 1900
HaeII RGCGCY 2 cut(s) 185, 2236
HaeIII GGCC 4 cut(s) 1050, 1205, 1401, 1456
HapII CCGG 5 cut(s) 306, 504, 681, 1154, 1402
HhaI GCGC 4 cut(s) 184, 1393, 1949, 2235
Hin6I GCGC 4 cut(s) 182, 1391, 1947, 2233
HinP1I GCGC 4 cut(s) 182, 1391, 1947, 2233
HincII GTYRAC 1 cut(s) 1513
HindII GTYRAC 1 cut(s) 1513
HindIII AAGCTT 3 cut(s) 670, 1329, 1859
HpaII CCGG 5 cut(s) 306, 504, 681, 1154, 1402
HphI GGTGA 4 cut(s) 121, 773, 1156, 1846
HpyAV CCTTC 4 cut(s) 93, 1177, 1603, 1813
HpyCH4III ACNGT 3 cut(s) 641, 761, 1139
HpyCH4IV ACGT 5 cut(s) 228, 382, 414, 1602, 1824
HpyF10VI GCNNNNNNNGC 9 cut(s) 272, 1043, 1052, 1328, 1388, 1417, 1890, 2047, 2166
HpyF3I CTNAG 8 cut(s) 16, 26, 792, 1180, 1207, 1233, 1407, 1830
HpySE526I ACGT 5 cut(s) 228, 382, 414, 1602, 1824
HspAI GCGC 4 cut(s) 182, 1391, 1947, 2233
Ksp22I TGATCA 1 cut(s) 2214
LguI GCTCTTC 2 cut(s) 1430, 2040
LmnI GCTCC 2 cut(s) 1881, 2038
Lsp1109I GCAGC 6 cut(s) 472, 754, 1405, 1432, 1720, 2110
LweI GCATC 2 cut(s) 786, 2157
MaeI CTAG 6 cut(s) 44, 167, 530, 627, 1283, 2012
MaeII ACGT 5 cut(s) 228, 382, 414, 1602, 1824
MaeIII GTNAC 6 cut(s) 19, 328, 1024, 1526, 2007, 2026
MboII GAAGA 8 cut(s) 71, 546, 1271, 1417, 1713, 1824, 1855, 2057
MflI RGATCY 3 cut(s) 1066, 1651, 1905
MhlI GDGCHC 1 cut(s) 382
MlyI GAGTC 5 cut(s) 224, 686, 1399, 1995, 2002
MmeI TCCRAC 2 cut(s) 793, 2087
MroXI GAANNNNTTC 2 cut(s) 1224, 2260
MslI CAYNNNNRTG 2 cut(s) 2153, 2263
MspI CCGG 5 cut(s) 306, 504, 681, 1154, 1402
MspR9I CCNGG 2 cut(s) 405, 505
Mva1269I GAATGC 1 cut(s) 1039
MvaI CCWGG 1 cut(s) 405
MwoI GCNNNNNNNGC 9 cut(s) 272, 1043, 1052, 1328, 1388, 1417, 1890, 2047, 2166
NciI CCSGG 1 cut(s) 505
NlaIV GGNNCC 2 cut(s) 310, 1653
NmeAIII GCCGAG 1 cut(s) 1466
NmuCI GTSAC 2 cut(s) 328, 2007
NsbI TGCGCA 1 cut(s) 1392
NspI RCATGY 3 cut(s) 290, 2116, 2262
PaeI GCATGC 1 cut(s) 290
PaeR7I CTCGAG 1 cut(s) 1171
PciI ACATGT 1 cut(s) 2258
PciSI GCTCTTC 2 cut(s) 1430, 2040
PctI GAATGC 1 cut(s) 1039
PdmI GAANNNNTTC 2 cut(s) 1224, 2260
PfeI GAWTC 5 cut(s) 366, 920, 1225, 1459, 2059
PkrI GCNGC 6 cut(s) 462, 769, 1395, 1422, 1735, 2125
PleI GAGTC 5 cut(s) 223, 685, 1399, 1994, 2001
PpsI GAGTC 5 cut(s) 223, 685, 1399, 1994, 2001
Ppu21I YACGTR 2 cut(s) 383, 1603
PscI ACATGT 1 cut(s) 2258
PshBI ATTAAT 1 cut(s) 978
Psp1406I AACGTT 1 cut(s) 414
Psp6I CCWGG 1 cut(s) 403
PspFI CCCAGC 1 cut(s) 1502
PspGI CCWGG 1 cut(s) 403
PspN4I GGNNCC 2 cut(s) 310, 1653
PspPI GGNCC 1 cut(s) 815
PstI CTGCAG 3 cut(s) 650, 1422, 1810
PsuI RGATCY 3 cut(s) 1066, 1651, 1905
RsaI GTAC 5 cut(s) 1094, 1295, 1601, 1799, 1968
RsaNI GTAC 5 cut(s) 1093, 1294, 1600, 1798, 1967
RseI CAYNNNNRTG 2 cut(s) 2153, 2263
SapI GCTCTTC 2 cut(s) 1430, 2040
SatI GCNGC 6 cut(s) 461, 768, 1394, 1421, 1734, 2124
Sau96I GGNCC 1 cut(s) 815
SchI GAGTC 5 cut(s) 224, 686, 1399, 1995, 2002
ScrFI CCNGG 2 cut(s) 405, 505
SduI GDGCHC 1 cut(s) 382
SfaNI GCATC 2 cut(s) 786, 2157
SfcI CTRYAG 3 cut(s) 646, 1418, 1806
Sfr274I CTCGAG 1 cut(s) 1171
SinI GGWCC 1 cut(s) 815
SlaI CTCGAG 1 cut(s) 1171
SmiMI CAYNNNNRTG 2 cut(s) 2153, 2263
SmlI CTYRAG 4 cut(s) 844, 1171, 1656, 1748
SmoI CTYRAG 4 cut(s) 844, 1171, 1656, 1748
SpeI ACTAGT 1 cut(s) 626
SphI GCATGC 1 cut(s) 290
SsiI CCGC 3 cut(s) 275, 1101, 2067
SspMI CTAG 6 cut(s) 44, 167, 530, 627, 1283, 2012
StyD4I CCNGG 2 cut(s) 403, 503
TaaI ACNGT 3 cut(s) 641, 761, 1139
TaiI ACGT 5 cut(s) 231, 385, 417, 1605, 1827
TatI WGTACW 1 cut(s) 1293
TfiI GAWTC 5 cut(s) 366, 920, 1225, 1459, 2059
TscAI CASTG 3 cut(s) 34, 1282, 1422
TseFI GTSAC 2 cut(s) 328, 2007
TseI GCWGC 6 cut(s) 460, 767, 1393, 1420, 1733, 2123
Tsp45I GTSAC 2 cut(s) 328, 2007
TspDTI ATGAA 6 cut(s) 881, 1217, 1556, 1862, 2058, 2253
TspGWI ACGGA 1 cut(s) 2268
TspRI CASTG 3 cut(s) 34, 1282, 1422
VneI GTGCAC 1 cut(s) 378
VpaK11BI GGWCC 1 cut(s) 815
VspI ATTAAT 1 cut(s) 978
XapI RAATTY 7 cut(s) 53, 73, 259, 1711, 1792, 1961, 2016
XbaI TCTAGA 1 cut(s) 166
XceI RCATGY 3 cut(s) 290, 2116, 2262
XhoI CTCGAG 1 cut(s) 1171
XmiI GTMKAC 3 cut(s) 250, 827, 1346
XmnI GAANNNNTTC 2 cut(s) 1224, 2260
XspI CTAG 6 cut(s) 44, 167, 530, 627, 1283, 2012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.