MD15G1444300.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010830
Physical Location & Seq
Forward (+)
54464525 .. 54467259
2735 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1444300.v1.1.491

Sequence Viewer

Length: 2685 bp
ATGATCTGCTACTTCGCGCGCAGGTCCCTCCACGTGTCCCGAACACCCCACTGGAAGCTCCGGGACGAGTACAAGCTCACCCGCCCCCAGTTACACGACCGCATATCCCGCCTCCTCCTCCTCCAGCGCTACGACGCCCTTCACCAGCTCTCCTTTGACTTCTCCGACCGCCTCCTCAGCACCGTCCTCCGCCAACTGAAGCTCAACCCCGCCGCCTGCTTAGCCTTTTTCAAATTGGCTTCCAAGCAGCACAAGTTCAGGCCCAACCTCAAGTCTTACTGTATGATTGTTCATATCTTGTCCAGAGCTGGAATGCACGACCAAACCAGAGCGTATTTGAACGAGTTGGTCCGTCTCTGCAACAACCATTGTTCAGCATTCGTGGTCTGGGATGAGCTTGTTCGCGTTTACAGGGAATTTACGTTTTCTCCAACGGTTTTCGACATGGTTCTCAAGGTGTTTGCTGAGAAGGGCATGACCAAGTGTGCACTTCATGTGTTTGATAATATGGGTAAGTGCGGTCGAGTTCCTAGTTTGAGGTCTTGTAATTCATTGTTGAGTAATTTGGTCAGAAACGGTGAATGTTTCAATGCATTGCTTGTGTATGAGCAGATAATTAGGTTGGGGATTGTTCCCGATGTTTATACCTGTTCGATAATGGTGAATGCGTATTGTAAGGAGGGGAGGCTGAGTAGAGCGGCGGAGTTTGTCAAAGAGATGGAAAGTTTGGGTTTTGAATTAAATGTGGTGACTTACAATAGTTTGATTAATGGGTACATCAGTTCGGGAGATGTTGAAGGAGCCCGATTGGTGTTGGGGTTGATGTCTGAGAAAGGAATTAAGAGAAATGTGGTGAGTTATACACTGTTGATTAAGGGTTACTGCAAGCAGTGTAAGATGGAGGAAGCGGAGAAGGTGCTTCGAGGTATGAAGGAGGAGGAGGGGGACGAGTTTGTGGTTGTGGATGAACTTGCTTATGGCGTCTTGTTAGATGGGTATTGTAAAGCTGGCAGAATTGATGATGCTATTAGGATTCGGGATGAGATGTTGACCATGGGTTTAAGTATGAATATTTTCATTTGCAACTCCTTGATCAATGGGTACTGCAAGGTCGGTCAAGTTCGTGACGCGGAGGGAGTGTTGTTGCGGATGAGATATTGGAACTTAAACCCTGATTCTTATAGTTATAATACCCTTATGGATGGGTATTGTAAGAAAGGTCAGACAAGTGATGCATTGAAGCTTTTTGATGAGATGCTTCAGGAAGGAATCCATCACACTGTTATAACTTATAATACGCTTCTCAAGGGTTTATGTCAAGCGGGTGCTTTTGATGATGCCTTGCATCTTTGGCATCTAATGTTGAAAAGAGGGTTGGCTCCTGACGAGGTTAGCTATTGTTCTCTGCTTGATGGGGTTTTGAAGAAGGAAGATCTTGATGGGGCTATAACTCTGTGGAAAGATATTCTAGCAAAGGGTTTTACCAAAAGTAAGTTTGCTTTCAATACAATGATTAATGGGTTATGCAAGATGGGAAAAATGGTTGAAGCGGAAGAGGTTTTCGAAAAGATGAAGGAGCTAGGATGTTTACCTGATGAGATGACTTATAGAACCCTGAGTGATGGGTACTGTAAAATTGGGAACGTTGAAGAAGCTTTCAAAGTTAAGAGTTTGATGGAAAGGCAAGCAATACTTCCTTCCATTGAAATGTACAATTCTCTCATCAATGGTGTCTTTATGTCCAGGAAATTAAGTAAAGTGAATGGTCTTCTTGCTGAGATGCAGACAAGGGGATTAACCCCGGATATTGTTACATATGGAGCCCTGATCACTGGTTGGTGCAATGAAGGGATGCTGGATAAAGCTTTGAGTTCATATTTTGAGATGATTGACAAAGGGTTCACCACCAATTTAATTATTTGCAGCAAAGTTGTCAGTACTCTGTATAGGCTTGGTAGGATTGATGAAGGAAATAGTCTGTTGCAGAAGCTATTGGATTTTGATTTTTTTTCAGATCAGCAATGTTTTTCGAAGCTTTGCAAAGTTGGCAGTAGAAATCAAGAAATTCAAAAATTTGCAGATTCTCTTGATGAAAGTGCTGAAAGTTTCTCTCTGTCCAACCCGGTTGTATACAATATTGCTATCTTGGGGCTCTGCAGATCAGGGAAAGTTGCTGATGCAAGGAAATTAATATCGTCATTATTGCTCAGTGGTATTTCTCCAGACAACTTTACTTATTGCACCCTAATCAATGCCACGGCTGCTGCTGGTAATGTGACTGAGGCTTTCAACTTACGAGATGAAATGCTGAAAGGGGATCTTGTTCCTAACATTACCACATATAATGCTCTTATAAATGGCTTGTCCAAATCAGGAAATTTGGATAGAGCACAGAGGCTTTTCCATAAACTTAACCGAAAGAAGTTAGTTCCTAATGATGTTACCTACAATATAATGATTGGTGGATACAGCAGAATTGGAAATACTGTGGAAGCGTTTAAATTTTGGAATGAGATGATTCGAAAAGGGATTGCTCCCTCTGTTATCACTTACTCTGCATTGATCAATGGTCTTTATAAGCAAGGAAATTTGGAAGAATCTGTGAAGCTTTTGAGTCAACTGATCAAGGTGGGAGTGCAGCATAACCTTGTTAATTACATACTCCAGTTTGGTTCAAAGCATTGA

Protein Analysis

895

Amino Acids

100.99

Weight (kDa)

8.57

Isoelectric Point (pI)

28.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 178 - 226 4.7e-08 PPR repeat family
PPR_3 PF13812 203 - 259 4.5e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 209 - 241 3.8e-09 PPR repeat
PPR PF01535 215 - 245 6e-06 PPR repeat
PPR_2 PF13041 219 - 259 3.9e-10 PPR repeat family
PPR_3 PF13812 237 - 294 1.7e-11 Pentatricopeptide repeat domain
PPR_2 PF13041 248 - 296 5.1e-14 PPR repeat family
PPR PF01535 250 - 280 1.9e-06 PPR repeat
PPR_1 PF12854 278 - 311 5.3e-09 PPR repeat
PPR_2 PF13041 282 - 313 3.5e-07 PPR repeat family
PPR PF01535 285 - 313 1.1e-06 PPR repeat
PPR_2 PF13041 322 - 358 5.4e-06 PPR repeat family
PPR_1 PF12854 322 - 349 2e-07 PPR repeat
PPR PF01535 325 - 354 6.9e-06 PPR repeat
PPR_3 PF13812 344 - 403 1.1e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 352 - 385 8.4e-06 PPR repeat
PPR_2 PF13041 357 - 402 5.1e-13 PPR repeat family
PPR PF01535 360 - 386 9.1e-06 PPR repeat
PPR_1 PF12854 388 - 419 2.5e-12 PPR repeat
PPR_2 PF13041 391 - 440 6.4e-19 PPR repeat family
PPR PF01535 394 - 424 1.7e-10 PPR repeat
PPR_long PF17177 400 - 547 2.8e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 414 - 471 1.3e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 423 - 454 6.9e-08 PPR repeat
PPR_2 PF13041 427 - 473 1.6e-14 PPR repeat family
PPR PF01535 429 - 459 1.1e-06 PPR repeat
PPR_1 PF12854 497 - 525 1.1e-09 PPR repeat
PPR_2 PF13041 499 - 545 2e-16 PPR repeat family
PPR PF01535 500 - 529 3.6e-10 PPR repeat
TPR_24 PF23276 517 - 627 2.3e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 528 - 560 6.5e-10 PPR repeat
PPR_2 PF13041 566 - 615 2.9e-10 PPR repeat family
PPR_1 PF12854 597 - 623 1.1e-07 PPR repeat
PPR_2 PF13041 601 - 637 3.3e-06 PPR repeat family
PPR_2 PF13041 707 - 753 2.4e-10 PPR repeat family
PPR_long PF17177 724 - 817 1e-05 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 737 - 786 1.6e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 738 - 769 2e-07 PPR repeat
PPR_2 PF13041 748 - 790 3.2e-09 PPR repeat family
PPR_3 PF13812 766 - 823 1.7e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 774 - 803 1.6e-08 PPR repeat
PPR_2 PF13041 776 - 824 3.2e-15 PPR repeat family
PPR PF01535 780 - 807 8e-06 PPR repeat
PPR_1 PF12854 808 - 839 1.6e-08 PPR repeat
PPR_2 PF13041 811 - 860 1.8e-17 PPR repeat family
PPR PF01535 814 - 844 7.7e-09 PPR repeat
PPR_1 PF12854 842 - 873 1.4e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010830)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19290 AT1G19290
fragaria_vesca FvH4_2g40380 FvH4_2g40380
malus_domestica MD15G1444300.v1.1
prunus_persica Prupe.1G585600_v2.0.a1
pyrus_communis pycom15g39140
rosa_chinensis RchiOBHm_Chr3g0486911 RchiOBHm_Chr6g0300361
rosa_laevigata RLG00000011344
rosa_multiflora Rmu_sc0001670.1_g000017
rosa_roxburghii Rroxscaffold_2G00128300 Rroxscaffold_7G00167860
rosa_rugosa Rorug06G0295300
rosa_samantha Rh3AG278100 Rh6AG408100 Rh6BG415400 Rh6CG422800 Rh6DG408400
rosa_wichuraiana Rw6G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 1188, 1286, 1293, 2354, 2577
Acc36I ACCTGC 1 cut(s) 12
AccBSI CCGCTC 1 cut(s) 698
AccI GTMKAC 1 cut(s) 2130
AccII CGCG 4 cut(s) 17, 19, 405, 1130
AclI AACGTT 1 cut(s) 1644
AclWI GGATC 1 cut(s) 2325
AcsI RAATTY 6 cut(s) 416, 2064, 2072, 2377, 2501, 2587
AcuI CTGAAG 2 cut(s) 218, 1244
AcvI CACGTG 1 cut(s) 34
AcyI GRCGYC 2 cut(s) 135, 981
AfaI GTAC 6 cut(s) 71, 776, 1103, 1628, 1712, 1939
AfeI AGCGCT 1 cut(s) 128
AflIII ACRYGT 1 cut(s) 33
AjnI CCWGG 1 cut(s) 1742
AjuI GAANNNNNNNTTGG 2 cut(s) 1358, 1390
Alw21I GWGCWC 2 cut(s) 490, 2392
Alw26I GTCTC 1 cut(s) 359
Alw44I GTGCAC 1 cut(s) 486
AlwI GGATC 1 cut(s) 2325
Aor51HI AGCGCT 1 cut(s) 128
AoxI GGCC 1 cut(s) 260
ApaLI GTGCAC 1 cut(s) 486
ApeKI GCWGC 5 cut(s) 247, 1923, 2261, 2264, 2638
ApoI RAATTY 6 cut(s) 416, 2064, 2072, 2377, 2501, 2587
AseI ATTAAT 3 cut(s) 768, 1515, 2189
Asp700I GAANNNNTTC 1 cut(s) 1073
AspLEI GCGC 3 cut(s) 19, 21, 129
AspS9I GGNCC 3 cut(s) 24, 261, 349
AsuC2I CCSGG 3 cut(s) 62, 1802, 2123
AsuHPI GGTGA 7 cut(s) 70, 134, 590, 673, 760, 865, 1894
AsuII TTCGAA 3 cut(s) 1563, 2030, 2521
AvaII GGWCC 2 cut(s) 24, 349
BaeGI GKGCMC 1 cut(s) 490
BaeI ACNNNNGTAYC 2 cut(s) 766, 799
BanII GRGCYC 3 cut(s) 805, 1825, 2154
BbrPI CACGTG 1 cut(s) 34
BbsI GAAGAC 1 cut(s) 1760
Bbv12I GWGCWC 2 cut(s) 490, 2392
BbvCI CCTCAGC 1 cut(s) 176
BbvI GCAGC 5 cut(s) 259, 1935, 2248, 2251, 2650
BceAI ACGGC 1 cut(s) 2274
BciT130I CCWGG 1 cut(s) 1744
BciVI GTATCC 1 cut(s) 2459
BclI TGATCA 4 cut(s) 1092, 1827, 2562, 2622
BcnI CCSGG 3 cut(s) 62, 1802, 2123
BcoDI GTCTC 1 cut(s) 359
BfaI CTAG 3 cut(s) 531, 1469, 1580
BfmI CTRYAG 1 cut(s) 2155
BfoI RGCGCY 1 cut(s) 130
BfuAI ACCTGC 1 cut(s) 12
BfuI GTATCC 1 cut(s) 2459
BglII AGATCT 1 cut(s) 1432
BisI GCNGC 7 cut(s) 213, 248, 699, 1924, 2262, 2265, 2639
BlpI GCTNAGC 1 cut(s) 220
BlsI GCNGC 7 cut(s) 214, 249, 700, 1925, 2263, 2266, 2640
BmcAI AGTACT 1 cut(s) 1939
Bme1390I CCNGG 4 cut(s) 62, 1744, 1802, 2123
Bme18I GGWCC 2 cut(s) 24, 349
BmgT120I GGNCC 3 cut(s) 24, 261, 349
BmiI GGNNCC 4 cut(s) 26, 802, 1380, 1822
BmrFI CCNGG 4 cut(s) 62, 1744, 1802, 2123
BmrI ACTGGG 1 cut(s) 82
BmsI GCATC 9 cut(s) 1012, 1222, 1245, 1327, 1354, 1363, 1770, 1842, 2167
BmuI ACTGGG 1 cut(s) 82
BpiI GAAGAC 1 cut(s) 1760
BpmI CTGGAG 3 cut(s) 107, 2205, 2648
Bpu10I CCTNAGC 1 cut(s) 176
Bpu1102I GCTNAGC 1 cut(s) 220
Bpu14I TTCGAA 3 cut(s) 1563, 2030, 2521
BpuEI CTTGAG 3 cut(s) 254, 437, 1289
BpuMI CCSGG 3 cut(s) 62, 1802, 2123
BsaAI YACGTR 1 cut(s) 34
BsaHI GRCGYC 2 cut(s) 135, 981
BsaJI CCNNGG 3 cut(s) 1053, 1800, 2256
BsaXI ACNNNNNCTCC 8 cut(s) 134, 164, 412, 442, 676, 706, 1124, 1154
Bse1I ACTGG 4 cut(s) 56, 88, 1837, 2665
Bse3DI GCAATG 3 cut(s) 593, 1849, 2027
BseBI CCWGG 1 cut(s) 1744
BseDI CCNNGG 3 cut(s) 1053, 1800, 2256
BseGI GGATG 7 cut(s) 397, 970, 1045, 1155, 1207, 1589, 1857
BseMI GCAATG 3 cut(s) 593, 1849, 2027
BseMII CTCAG 8 cut(s) 190, 456, 680, 819, 1607, 1767, 2221, 2271
BseNI ACTGG 4 cut(s) 56, 88, 1837, 2665
BsePI GCGCGC 1 cut(s) 17
BseRI GAGGAG 6 cut(s) 104, 107, 110, 164, 950, 953
BseSI GKGCMC 1 cut(s) 490
BseXI GCAGC 5 cut(s) 259, 1935, 2248, 2251, 2650
BsgI GTGCAG 1 cut(s) 2657
Bsh1236I CGCG 4 cut(s) 17, 19, 405, 1130
Bsh1285I CGRYCG 3 cut(s) 100, 169, 523
BshFI GGCC 1 cut(s) 262
BsiEI CGRYCG 3 cut(s) 100, 169, 523
BsiHKAI GWGCWC 2 cut(s) 490, 2392
BsiSI CCGG 3 cut(s) 61, 1802, 2123
BslFI GGGAC 4 cut(s) 10, 22, 77, 959
BsmAI GTCTC 1 cut(s) 359
BsmBI CGTCTC 1 cut(s) 359
BsmFI GGGAC 4 cut(s) 10, 22, 77, 959
BsmI GAATGC 3 cut(s) 318, 377, 670
BsnI GGCC 1 cut(s) 262
Bsp119I TTCGAA 3 cut(s) 1563, 2030, 2521
Bsp1286I GDGCHC 5 cut(s) 490, 805, 1825, 2154, 2392
Bsp1407I TGTACA 1 cut(s) 1710
Bsp143I GATC 9 cut(s) 3, 1092, 1432, 1827, 2014, 2159, 2317, 2562, 2622
Bsp1720I GCTNAGC 1 cut(s) 220
Bsp19I CCATGG 1 cut(s) 1053
BspANI GGCC 1 cut(s) 262
BspCNI CTCAG 8 cut(s) 189, 457, 681, 820, 1608, 1768, 2220, 2272
BspFNI CGCG 4 cut(s) 17, 19, 405, 1130
BspLI GGNNCC 4 cut(s) 26, 802, 1380, 1822
BspMAI CTGCAG 1 cut(s) 2159
BspMI ACCTGC 1 cut(s) 12
BspPI GGATC 1 cut(s) 2325
BspT104I TTCGAA 3 cut(s) 1563, 2030, 2521
BsrBI CCGCTC 1 cut(s) 698
BsrDI GCAATG 3 cut(s) 593, 1849, 2027
BsrGI TGTACA 1 cut(s) 1710
BsrI ACTGG 4 cut(s) 56, 88, 1837, 2665
BssECI CCNNGG 3 cut(s) 1053, 1800, 2256
BssHII GCGCGC 1 cut(s) 17
BssMI GATC 9 cut(s) 3, 1092, 1432, 1827, 2014, 2159, 2317, 2562, 2622
BssNAI GTATAC 1 cut(s) 2131
BssNI GRCGYC 2 cut(s) 135, 981
BssT1I CCWWGG 1 cut(s) 1053
Bst1107I GTATAC 1 cut(s) 2131
Bst2UI CCWGG 1 cut(s) 1744
Bst4CI ACNGT 8 cut(s) 184, 281, 436, 578, 867, 1282, 1631, 2488
Bst6I CTCTTC 1 cut(s) 1548
BstACI GRCGYC 2 cut(s) 135, 981
BstAUI TGTACA 1 cut(s) 1710
BstBAI YACGTR 1 cut(s) 34
BstBI TTCGAA 3 cut(s) 1563, 2030, 2521
BstC8I GCNNGC 5 cut(s) 19, 217, 887, 1009, 1686
BstDEI CTNAG 9 cut(s) 176, 220, 465, 689, 828, 1616, 1776, 2207, 2280
BstDSI CCRYGG 2 cut(s) 1053, 2256
BstF5I GGATG 7 cut(s) 397, 970, 1045, 1155, 1207, 1589, 1857
BstFNI CGCG 4 cut(s) 17, 19, 405, 1130
BstH2I RGCGCY 1 cut(s) 130
BstHHI GCGC 3 cut(s) 19, 21, 129
BstKTI GATC 9 cut(s) 6, 1095, 1435, 1830, 2017, 2162, 2320, 2565, 2625
BstMAI GTCTC 1 cut(s) 359
BstMBI GATC 9 cut(s) 3, 1092, 1432, 1827, 2014, 2159, 2317, 2562, 2622
BstMCI CGRYCG 3 cut(s) 100, 169, 523
BstMWI GCNNNNNNNGC 6 cut(s) 108, 177, 221, 1351, 2046, 2261
BstNI CCWGG 1 cut(s) 1744
BstSCI CCNGG 4 cut(s) 60, 1742, 1800, 2121
BstSFI CTRYAG 1 cut(s) 2155
BstSLI GKGCMC 1 cut(s) 490
BstUI CGCG 4 cut(s) 17, 19, 405, 1130
BstV1I GCAGC 5 cut(s) 259, 1935, 2248, 2251, 2650
BstV2I GAAGAC 1 cut(s) 1760
BstX2I RGATCY 2 cut(s) 1432, 2317
BstYI RGATCY 2 cut(s) 1432, 2317
BstZ17I GTATAC 1 cut(s) 2131
BsuI GTATCC 1 cut(s) 2459
BsuRI GGCC 1 cut(s) 262
BtgI CCRYGG 2 cut(s) 1053, 2256
BtsCI GGATG 7 cut(s) 397, 970, 1045, 1155, 1207, 1589, 1857
BtsI GCAGTG 1 cut(s) 896
BtsIMutI CAGTG 6 cut(s) 49, 863, 896, 1278, 1830, 2215
BveI ACCTGC 1 cut(s) 12
Cac8I GCNNGC 5 cut(s) 19, 217, 887, 1009, 1686
CfoI GCGC 3 cut(s) 19, 21, 129
Cfr13I GGNCC 3 cut(s) 24, 261, 349
CseI GACGC 3 cut(s) 143, 970, 1136
Csp6I GTAC 6 cut(s) 70, 775, 1102, 1627, 1711, 1938
CviAII CATG 4 cut(s) 445, 475, 494, 1054
CviQI GTAC 6 cut(s) 70, 775, 1102, 1627, 1711, 1938
DdeI CTNAG 9 cut(s) 176, 220, 465, 689, 828, 1616, 1776, 2207, 2280
DpnI GATC 9 cut(s) 5, 1094, 1434, 1829, 2016, 2161, 2319, 2564, 2624
DpnII GATC 9 cut(s) 3, 1092, 1432, 1827, 2014, 2159, 2317, 2562, 2622
DraI TTTAAA 1 cut(s) 2500
Eam1104I CTCTTC 1 cut(s) 1548
EarI CTCTTC 1 cut(s) 1548
EciI GGCGGA 2 cut(s) 179, 716
Eco130I CCWWGG 1 cut(s) 1053
Eco24I GRGCYC 3 cut(s) 805, 1825, 2154
Eco47I GGWCC 2 cut(s) 24, 349
Eco47III AGCGCT 1 cut(s) 128
Eco57I CTGAAG 2 cut(s) 218, 1244
Eco72I CACGTG 1 cut(s) 34
EcoO109I RGGNCCY 1 cut(s) 24
EcoRII CCWGG 1 cut(s) 1742
EcoT14I CCWWGG 1 cut(s) 1053
EcoT22I ATGCAT 2 cut(s) 595, 1237
EcoT38I GRGCYC 3 cut(s) 805, 1825, 2154
ErhI CCWWGG 1 cut(s) 1053
Esp3I CGTCTC 1 cut(s) 359
FaeI CATG 4 cut(s) 448, 478, 497, 1057
FalI AAGNNNNNCTT 4 cut(s) 1677, 1709, 2304, 2336
FaqI GGGAC 4 cut(s) 10, 22, 77, 959
FatI CATG 4 cut(s) 444, 474, 493, 1053
FauI CCCGC 4 cut(s) 89, 116, 217, 1315
FauNDI CATATG 1 cut(s) 1816
FbaI TGATCA 4 cut(s) 1092, 1827, 2562, 2622
FblI GTMKAC 1 cut(s) 2130
Fnu4HI GCNGC 7 cut(s) 213, 248, 699, 1924, 2262, 2265, 2639
FokI GGATG 7 cut(s) 404, 977, 1052, 1162, 1214, 1596, 1864
FriOI GRGCYC 3 cut(s) 805, 1825, 2154
Fsp4HI GCNGC 7 cut(s) 213, 248, 699, 1924, 2262, 2265, 2639
FspBI CTAG 3 cut(s) 531, 1469, 1580
GlaI GCGC 3 cut(s) 18, 20, 128
GluI GCNGC 7 cut(s) 213, 248, 699, 1924, 2262, 2265, 2639
GsuI CTGGAG 3 cut(s) 107, 2205, 2648
HaeII RGCGCY 1 cut(s) 130
HaeIII GGCC 1 cut(s) 262
HapII CCGG 3 cut(s) 61, 1802, 2123
HgaI GACGC 3 cut(s) 143, 970, 1136
HhaI GCGC 3 cut(s) 19, 21, 129
Hin1I GRCGYC 2 cut(s) 135, 981
Hin1II CATG 4 cut(s) 448, 478, 497, 1057
Hin6I GCGC 3 cut(s) 17, 19, 127
HinP1I GCGC 3 cut(s) 17, 19, 127
HincII GTYRAC 2 cut(s) 1050, 2618
HindII GTYRAC 2 cut(s) 1050, 2618
HindIII AAGCTT 5 cut(s) 1241, 1653, 1863, 2033, 2606
HinfI GANTC 7 cut(s) 1033, 1175, 1269, 2081, 2518, 2597, 2614
HpaII CCGG 3 cut(s) 61, 1802, 2123
HphI GGTGA 7 cut(s) 70, 134, 590, 673, 760, 865, 1894
Hpy166II GTNNAC 7 cut(s) 409, 488, 1050, 1589, 1902, 2131, 2618
Hpy188I TCNGA 5 cut(s) 166, 572, 829, 1224, 2014
Hpy8I GTNNAC 7 cut(s) 409, 488, 1050, 1589, 1902, 2131, 2618
Hpy99I CGWCG 1 cut(s) 137
HpyCH4III ACNGT 8 cut(s) 184, 281, 436, 578, 867, 1282, 1631, 2488
HpyCH4IV ACGT 3 cut(s) 33, 422, 1644
HpyF10VI GCNNNNNNNGC 6 cut(s) 108, 177, 221, 1351, 2046, 2261
HpyF3I CTNAG 9 cut(s) 176, 220, 465, 689, 828, 1616, 1776, 2207, 2280
HpySE526I ACGT 3 cut(s) 33, 422, 1644
Hsp92I GRCGYC 2 cut(s) 135, 981
Hsp92II CATG 4 cut(s) 448, 478, 497, 1057
HspAI GCGC 3 cut(s) 17, 19, 127
Ksp22I TGATCA 4 cut(s) 1092, 1827, 2562, 2622
Kzo9I GATC 9 cut(s) 3, 1092, 1432, 1827, 2014, 2159, 2317, 2562, 2622
LmnI GCTCC 6 cut(s) 63, 800, 1384, 1576, 1820, 2539
Lsp1109I GCAGC 5 cut(s) 259, 1935, 2248, 2251, 2650
LweI GCATC 9 cut(s) 1012, 1222, 1245, 1327, 1354, 1363, 1770, 1842, 2167
MaeI CTAG 3 cut(s) 531, 1469, 1580
MaeII ACGT 3 cut(s) 33, 422, 1644
MaeIII GTNAC 7 cut(s) 90, 748, 878, 1124, 1810, 2275, 2440
MalI GATC 9 cut(s) 5, 1094, 1434, 1829, 2016, 2161, 2319, 2564, 2624
MbiI CCGCTC 1 cut(s) 698
MboI GATC 9 cut(s) 3, 1092, 1432, 1827, 2014, 2159, 2317, 2562, 2622
MboII GAAGA 6 cut(s) 1435, 1442, 1565, 1661, 1760, 2606
MflI RGATCY 2 cut(s) 1432, 2317
MhlI GDGCHC 5 cut(s) 490, 805, 1825, 2154, 2392
MlyI GAGTC 1 cut(s) 2623
MmeI TCCRAC 3 cut(s) 189, 455, 2142
Mph1103I ATGCAT 2 cut(s) 595, 1237
MroXI GAANNNNTTC 1 cut(s) 1073
MslI CAYNNNNRTG 1 cut(s) 1706
MspI CCGG 3 cut(s) 61, 1802, 2123
MspR9I CCNGG 4 cut(s) 62, 1744, 1802, 2123
Mva1269I GAATGC 3 cut(s) 318, 377, 670
MvaI CCWGG 1 cut(s) 1744
MvnI CGCG 4 cut(s) 17, 19, 405, 1130
MwoI GCNNNNNNNGC 6 cut(s) 108, 177, 221, 1351, 2046, 2261
NciI CCSGG 3 cut(s) 62, 1802, 2123
NcoI CCATGG 1 cut(s) 1053
NdeI CATATG 1 cut(s) 1816
NdeII GATC 9 cut(s) 3, 1092, 1432, 1827, 2014, 2159, 2317, 2562, 2622
NlaIII CATG 4 cut(s) 448, 478, 497, 1057
NlaIV GGNNCC 4 cut(s) 26, 802, 1380, 1822
NmuCI GTSAC 3 cut(s) 748, 1124, 2275
NsiI ATGCAT 2 cut(s) 595, 1237
NspV TTCGAA 3 cut(s) 1563, 2030, 2521
PauI GCGCGC 1 cut(s) 17
PctI GAATGC 3 cut(s) 318, 377, 670
PdmI GAANNNNTTC 1 cut(s) 1073
PfeI GAWTC 6 cut(s) 1033, 1175, 1269, 2081, 2518, 2597
PfoI TCCNGGA 2 cut(s) 60, 1742
PkrI GCNGC 7 cut(s) 214, 249, 700, 1925, 2263, 2266, 2640
PleI GAGTC 1 cut(s) 2622
PmaCI CACGTG 1 cut(s) 34
PmlI CACGTG 1 cut(s) 34
PpsI GAGTC 1 cut(s) 2622
Ppu21I YACGTR 1 cut(s) 34
PpuMI RGGWCCY 1 cut(s) 24
PshBI ATTAAT 3 cut(s) 768, 1515, 2189
PsiI TTATAA 5 cut(s) 1188, 1286, 1293, 2354, 2577
Psp1406I AACGTT 1 cut(s) 1644
Psp5II RGGWCCY 1 cut(s) 24
Psp6I CCWGG 1 cut(s) 1742
PspCI CACGTG 1 cut(s) 34
PspGI CCWGG 1 cut(s) 1742
PspN4I GGNNCC 4 cut(s) 26, 802, 1380, 1822
PspPI GGNCC 3 cut(s) 24, 261, 349
PspPPI RGGWCCY 1 cut(s) 24
PstI CTGCAG 1 cut(s) 2159
PsuI RGATCY 2 cut(s) 1432, 2317
PteI GCGCGC 1 cut(s) 17
RsaI GTAC 6 cut(s) 71, 776, 1103, 1628, 1712, 1939
RsaNI GTAC 6 cut(s) 70, 775, 1102, 1627, 1711, 1938
RseI CAYNNNNRTG 1 cut(s) 1706
SatI GCNGC 7 cut(s) 213, 248, 699, 1924, 2262, 2265, 2639
Sau3AI GATC 9 cut(s) 3, 1092, 1432, 1827, 2014, 2159, 2317, 2562, 2622
Sau96I GGNCC 3 cut(s) 24, 261, 349
ScaI AGTACT 1 cut(s) 1939
SchI GAGTC 1 cut(s) 2623
ScrFI CCNGG 4 cut(s) 62, 1744, 1802, 2123
SduI GDGCHC 5 cut(s) 490, 805, 1825, 2154, 2392
SfaNI GCATC 9 cut(s) 1012, 1222, 1245, 1327, 1354, 1363, 1770, 1842, 2167
SfcI CTRYAG 1 cut(s) 2155
SfuI TTCGAA 3 cut(s) 1563, 2030, 2521
SinI GGWCC 2 cut(s) 24, 349
SmiMI CAYNNNNRTG 1 cut(s) 1706
SmlI CTYRAG 3 cut(s) 269, 452, 1304
SmoI CTYRAG 3 cut(s) 269, 452, 1304
SspI AATATT 2 cut(s) 1072, 2137
SspMI CTAG 3 cut(s) 531, 1469, 1580
StyD4I CCNGG 4 cut(s) 60, 1742, 1800, 2121
StyI CCWWGG 1 cut(s) 1053
TaaI ACNGT 8 cut(s) 184, 281, 436, 578, 867, 1282, 1631, 2488
TaiI ACGT 3 cut(s) 36, 425, 1647
TaqI TCGA 7 cut(s) 441, 523, 653, 922, 1563, 2030, 2521
TaqII GACCGA 1 cut(s) 1103
TatI WGTACW 3 cut(s) 69, 1710, 1937
TauI GCSGC 2 cut(s) 215, 701
TfiI GAWTC 6 cut(s) 1033, 1175, 1269, 2081, 2518, 2597
TscAI CASTG 6 cut(s) 56, 870, 896, 1285, 1837, 2215
TseFI GTSAC 3 cut(s) 748, 1124, 2275
TseI GCWGC 5 cut(s) 247, 1923, 2261, 2264, 2638
Tsp45I GTSAC 3 cut(s) 748, 1124, 2275
TspGWI ACGGA 1 cut(s) 341
TspRI CASTG 6 cut(s) 56, 870, 896, 1285, 1837, 2215
VneI GTGCAC 1 cut(s) 486
VpaK11BI GGWCC 2 cut(s) 24, 349
VspI ATTAAT 3 cut(s) 768, 1515, 2189
XapI RAATTY 6 cut(s) 416, 2064, 2072, 2377, 2501, 2587
XmiI GTMKAC 1 cut(s) 2130
XmnI GAANNNNTTC 1 cut(s) 1073
XspI CTAG 3 cut(s) 531, 1469, 1580
ZrmI AGTACT 1 cut(s) 1939
Zsp2I ATGCAT 2 cut(s) 595, 1237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.