RchiOBHm_Chr6g0300361

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0010830
Physical Location & Seq
Forward (+)
61230406 .. 61235673
5268 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26974

Sequence Viewer

Length: 2568 bp
ATGCTCAGGCGCTACTGCTTCCACCGCCGACGACTCCACGTGTCCAGAACCGGACTCCACTGGAAGCCCCGGGACGAGTACAAGCTGACCCGACCCGAATTGCTCGACCGGATCTCCCGCCTCCTCGTCCTCCAACGGTACGACGCCCTTAACAACCTCTCTTTTGAATTCTCCGACCAACTCCTCAACACCGTTCTCCGGAAACTGAAGCTAAACCCTAACGCCTGCTTAGAGTTTTTCAAATTGGCCTCGAAGCAACAGAAATTCAGGCCCAATCTCAAGTCCTACTGTATAATTGTTCATATTTTGTCTCGTGCTCGAATGCACGATCAAACCAGAGCCTATTTGAATGAATTGGTTGCTCTCTGTAAGAGTAATTATCCGGCTTTCGTGGTCTGGAATGAGCTTGTTAGGGTTTATAGGGAGTTTACATTTTCTCCCACGGTTTTCGATATGATTCTCAAGGTGTTTGCTGAACAAGGCATGACAAAGTATGCGTTACATGTCTTTGATAATATGGGCAAGTGCGGTAGGGTGCCGAGTTTGAGGTCTTGTAATTCTTTGTTGAGTAATTTAGTCAGGAGTGGTGAATGTCACACTGCATTGCTTGTTTATGAGCAGATAGTTAGGTTGGGGATTGTTCCGGATGTTTATACGTGTTCGATAGTGGTGAGAGCATATTGTAAAGAGGGGAGAGTGAGCAGAGCGGCGGAGTTTGTGAAAGAAATGGAGAGTTCGGGTTTTGAGTTGAATGTAGTGACTTACAATTGTTTGATTGATGGGTATGCTGGTCTGGGAGATGTTGGAGGAGCGAAATCGGTGTTGAGGTTGATGTCTGAAAGGGGAATTAAGAGAAATGTGGTGAGTTGTACACTGTTGATGAAGGCTTACTGCAAGCAAGGTAAGATGGAGGAAGCGGAGGAGGTGCTTCGCGGTATAAAGGAGGAGGAGCCAGTGGTTGTGGATGAGCGTGCATATGGTGTGTTGGTAGATGGATATTGTAAAGCTGGCAGAATGGATGATGCTAGTAGGATACATGATGAGTTGTTGAGGATTGGTTTGAAAATGAATACTATCATTTGCAACTCCTTGATCAACGGGTATTGTAAGCTTGGTCAAGTTTGGGAAGCAGAGGGAGTATTGCAGCGTATGAGATCTTGGAACTTAAAGCCAGATTCTTATAGTTACAATACCCTGATGGATGGTTACTGTAGGAAAGGTCAGACGAGTGAAGCATTGAAGCTTTTCGATGAGATGCCTGAGGGAGGAATCCATCAAACTGTGGTAACTTATAATACCATTCTCAAGGGTTTATGTCAAGCAAATGCTTTTGATGATGCTTTACACCTTTGGCATTTAATGTTGAAAAGAGGGTTGGCTCCTGATGAGGTTAGCTACTGTTCTCTGCTTGATGGGTTTTTCAAGAAGGAAGATCTTGACGGCGCTATAACTCTGTGGAAAGATATTTTGGCAAAGGGTTTTACCAAAAGCCGGTTTGCTTTCAATACAATGATTAATGGGTTATGCAAGATGGGGAAATTGGTTGAAGCGGAGGAGATCTTCGATAAGATGAAGGAGCTAGGATATTTACCTGATGAGATAACTTATAGAACCCTGAGTGATGGGTACTGCAAACTTGGGAATGTTGAAGAAGCTTTCAGAGTTAAAACTTTGATGGAAGGCCAGGCAATATTTCCTTCGATTGAAATGTACAATTCTCTCATCAGTGGTGTTTTTATGTCTAGAAATATAAGTAAAGTGATGAATCTTCTTGCTGAGATGCAGACAAGGGGACTATCCCCTAATACTGTAACATATGGAGCCCTTATCTCTGGTTGCAAATTTATCAGTACTCTGTATAGGCTTGGAAGGATTGATGAAGCAAGTATTCTGTTGCAGAAGTTAGTACATTATGATCCTTTTCCAGTTCAAAAAGGTGATATTACACAACGCGAAATTCAAAAGTTTGCAGATTCTCTTGATGAAAGTGCTATAAGTGTCTGTCTTCCCAACAATGTATTGTACAACATTGCCATCTTTGGAATCTGCAAATCGGGGAAGGTTGGTGATGCAAGAAGATTTTTATCAGCTTTGTTACTTAGTGGCTTTTCTCCAGACAATTTCACATATTGTACCCTGATTCATGCCACTGCTGCTGCTGGTAATGTGAACGAGGCTTTCAGCTTACGAGATGAAATGCTGAGAAGGAATCTTGTTCCTAACATTACCACATATAATGCTCTTATAAATGGCTTGTGCAAAGTAGGAGAATTGGATCGAGCGCAGAGGCTTTTCTATAAACTTTGCAAGAAGGGGTTAGCTCCTAATGCTGTTACCTATAATATATTGATTGATGGATACTGCAGAACTGGCAATACTGTTGAAGCTTTTAAATTTAAAGACGAGATGATTCTAGAAGGGATTGTTCCCTCTATAATCACTTATTCTGCATTGATCAATGGTCTTTATAAGCAAGGAAATATGGAAGAATCTGTGAAGCTTTTGAGCCAAATGATCAAGGCTGGAGTGCAACAAAACCTAGTCAGTTATGTACTCCAGTTTAGTTGA

Protein Analysis

855

Amino Acids

96.85

Weight (kDa)

8.76

Isoelectric Point (pI)

38.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 181 - 229 3.4e-08 PPR repeat family
PPR_3 PF13812 205 - 262 2.3e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 212 - 244 5.5e-08 PPR repeat
PPR_2 PF13041 223 - 263 2.5e-10 PPR repeat family
TPR_24 PF23276 223 - 347 1.3e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 247 - 278 6.5e-06 PPR repeat
PPR_2 PF13041 251 - 288 2.8e-07 PPR repeat family
PPR PF01535 253 - 283 8.2e-06 PPR repeat
PPR_3 PF13812 273 - 316 6.7e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 321 - 348 3.3e-06 PPR repeat
PPR_3 PF13812 344 - 401 4.6e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 353 - 385 6.4e-07 PPR repeat
PPR_2 PF13041 357 - 404 2.3e-15 PPR repeat family
PPR PF01535 360 - 388 3.3e-07 PPR repeat
PPR_3 PF13812 380 - 436 1.7e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 388 - 419 5.7e-13 PPR repeat
PPR_2 PF13041 394 - 431 1.4e-11 PPR repeat family
PPR PF01535 394 - 424 2.8e-10 PPR repeat
PPR_1 PF12854 423 - 454 6.7e-07 PPR repeat
PPR_2 PF13041 427 - 475 2.3e-15 PPR repeat family
PPR_long PF17177 475 - 611 2.7e-07 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 497 - 525 1.1e-09 PPR repeat
PPR_2 PF13041 499 - 545 2e-15 PPR repeat family
PPR PF01535 500 - 528 1.2e-09 PPR repeat
PPR_1 PF12854 528 - 560 4.3e-09 PPR repeat
PPR_3 PF13812 555 - 613 4.4e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 566 - 613 7.4e-11 PPR repeat family
PPR_2 PF13041 670 - 716 4.3e-09 PPR repeat family
PPR_long PF17177 690 - 793 1e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 702 - 733 1.3e-07 PPR repeat
PPR_3 PF13812 702 - 750 2.2e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 712 - 754 3e-12 PPR repeat family
PPR_3 PF13812 729 - 787 7.8e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 736 - 765 1.1e-10 PPR repeat
PPR_2 PF13041 740 - 789 2.6e-18 PPR repeat family
PPR PF01535 744 - 773 1.2e-06 PPR repeat
PPR_1 PF12854 771 - 803 2e-13 PPR repeat
PPR_2 PF13041 775 - 824 2.1e-16 PPR repeat family
PPR PF01535 778 - 808 6.6e-08 PPR repeat
PPR_1 PF12854 807 - 838 3.4e-07 PPR repeat
PPR_2 PF13041 810 - 850 3.2e-08 PPR repeat family
PPR PF01535 813 - 843 1.8e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010830)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19290 AT1G19290
fragaria_vesca FvH4_2g40380 FvH4_2g40380
malus_domestica MD15G1444300.v1.1
prunus_persica Prupe.1G585600_v2.0.a1
pyrus_communis pycom15g39140
rosa_chinensis RchiOBHm_Chr3g0486911 RchiOBHm_Chr6g0300361
rosa_laevigata RLG00000011344
rosa_multiflora Rmu_sc0001670.1_g000017
rosa_roxburghii Rroxscaffold_2G00128300 Rroxscaffold_7G00167860
rosa_rugosa Rorug06G0295300
rosa_samantha Rh3AG278100 Rh6AG408100 Rh6BG415400 Rh6CG422800 Rh6DG408400
rosa_wichuraiana Rw6G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1293, 2246, 2469
AccB1I GGYRCC 1 cut(s) 535
AccBSI CCGCTC 1 cut(s) 707
AccII CGCG 2 cut(s) 933, 1953
AccIII TCCGGA 2 cut(s) 198, 643
AciI CCGC 8 cut(s) 25, 118, 528, 707, 710, 917, 933, 1550
AclWI GGATC 3 cut(s) 119, 1910, 2283
AcsI RAATTY 5 cut(s) 167, 263, 1841, 1956, 2393
AcuI CTGAAG 1 cut(s) 227
AcvI CACGTG 1 cut(s) 40
AcyI GRCGYC 1 cut(s) 144
AfiI CCNNNNNNNGG 3 cut(s) 198, 1265, 1491
AflIII ACRYGT 3 cut(s) 39, 502, 656
AjnI CCWGG 1 cut(s) 1683
AjuI GAANNNNNNNTTGG 4 cut(s) 1358, 1390, 1451, 1483
Alw21I GWGCWC 1 cut(s) 319
Alw26I GTCTC 1 cut(s) 315
AlwI GGATC 3 cut(s) 119, 1910, 2283
Ama87I CYCGRG 1 cut(s) 69
Aor13HI TCCGGA 2 cut(s) 198, 643
AoxI GGCC 3 cut(s) 246, 269, 1681
ApeKI GCWGC 3 cut(s) 1144, 2153, 2156
ApoI RAATTY 5 cut(s) 167, 263, 1841, 1956, 2393
AseI ATTAAT 1 cut(s) 1515
Asp700I GAANNNNTTC 1 cut(s) 1244
AspLEI GCGC 3 cut(s) 12, 1445, 2284
AspS9I GGNCC 1 cut(s) 270
AsuC2I CCSGG 2 cut(s) 70, 71
AsuHPI GGTGA 5 cut(s) 599, 682, 874, 1949, 2078
AvaI CYCGRG 1 cut(s) 69
AxyI CCTNAGG 1 cut(s) 1260
BanI GGYRCC 1 cut(s) 535
BanII GRGCYC 1 cut(s) 1825
BauI CACGAG 1 cut(s) 312
BbrPI CACGTG 1 cut(s) 40
BbsI GAAGAC 1 cut(s) 1997
Bbv12I GWGCWC 1 cut(s) 319
BbvI GCAGC 3 cut(s) 1156, 2140, 2143
BceAI ACGGC 1 cut(s) 1456
BciT130I CCWGG 1 cut(s) 1685
BciVI GTATCC 2 cut(s) 1026, 2351
BclI TGATCA 3 cut(s) 1092, 2454, 2514
BcnI CCSGG 2 cut(s) 70, 71
BcoDI GTCTC 1 cut(s) 315
BfaI CTAG 5 cut(s) 1026, 1580, 1743, 2414, 2540
BfmI CTRYAG 2 cut(s) 1210, 2362
BfoI RGCGCY 2 cut(s) 13, 1446
BfuI GTATCC 2 cut(s) 1026, 2351
BglII AGATCT 3 cut(s) 1154, 1432, 1557
BisI GCNGC 4 cut(s) 708, 1145, 2154, 2157
BlsI GCNGC 4 cut(s) 709, 1146, 2155, 2158
BmcAI AGTACT 1 cut(s) 1852
Bme1390I CCNGG 3 cut(s) 70, 71, 1685
BmeT110I CYCGRG 1 cut(s) 69
BmgT120I GGNCC 1 cut(s) 270
BmiI GGNNCC 4 cut(s) 537, 951, 1380, 1822
BmrFI CCNGG 3 cut(s) 70, 71, 1685
BmsI GCATC 5 cut(s) 1012, 1245, 1327, 1770, 2059
BpiI GAAGAC 1 cut(s) 1997
BpmI CTGGAG 3 cut(s) 2097, 2540, 2544
Bpu10I CCTNAGC 1 cut(s) 5
BpuEI CTTGAG 3 cut(s) 263, 446, 1289
BpuMI CCSGG 2 cut(s) 70, 71
BsaAI YACGTR 2 cut(s) 40, 657
BsaBI GATNNNNATC 1 cut(s) 2083
BsaHI GRCGYC 1 cut(s) 144
BsaJI CCNNGG 3 cut(s) 68, 69, 441
BsaWI WCCGGW 4 cut(s) 50, 108, 198, 643
BsaXI ACNNNNNCTCC 4 cut(s) 98, 128, 421, 451
Bsc4I CCNNNNNNNGG 3 cut(s) 198, 1265, 1491
Bse118I RCCGGY 1 cut(s) 1491
Bse1I ACTGG 5 cut(s) 65, 953, 1925, 2374, 2557
Bse21I CCTNAGG 1 cut(s) 1260
Bse3DI GCAATG 2 cut(s) 602, 2028
Bse8I GATNNNNATC 1 cut(s) 2083
BseAI TCCGGA 2 cut(s) 198, 643
BseBI CCWGG 1 cut(s) 1685
BseDI CCNNGG 3 cut(s) 68, 69, 441
BseGI GGATG 4 cut(s) 652, 970, 1024, 1207
BseJI GATNNNNATC 1 cut(s) 2083
BseLI CCNNNNNNNGG 3 cut(s) 198, 1265, 1491
BseMI GCAATG 2 cut(s) 602, 2028
BseMII CTCAG 5 cut(s) 19, 1251, 1607, 1767, 2192
BseNI ACTGG 5 cut(s) 65, 953, 1925, 2374, 2557
BseRI GAGGAG 7 cut(s) 113, 173, 822, 935, 959, 962, 1568
BseXI GCAGC 3 cut(s) 1156, 2140, 2143
Bsh1236I CGCG 2 cut(s) 933, 1953
Bsh1285I CGRYCG 1 cut(s) 109
BshFI GGCC 3 cut(s) 248, 271, 1683
BshNI GGYRCC 1 cut(s) 535
BsiEI CGRYCG 1 cut(s) 109
BsiHKAI GWGCWC 1 cut(s) 319
BsiHKCI CYCGRG 1 cut(s) 69
BsiSI CCGG 7 cut(s) 51, 70, 109, 199, 383, 644, 1492
BslFI GGGAC 2 cut(s) 86, 1806
BslI CCNNNNNNNGG 3 cut(s) 198, 1265, 1491
BsmAI GTCTC 1 cut(s) 315
BsmFI GGGAC 2 cut(s) 86, 1806
BsmI GAATGC 1 cut(s) 327
BsnI GGCC 3 cut(s) 248, 271, 1683
BsoBI CYCGRG 1 cut(s) 69
Bsp1286I GDGCHC 2 cut(s) 319, 1825
Bsp13I TCCGGA 2 cut(s) 198, 643
Bsp1407I TGTACA 3 cut(s) 869, 1710, 2022
BspACI CCGC 8 cut(s) 25, 118, 528, 707, 710, 917, 933, 1550
BspANI GGCC 3 cut(s) 248, 271, 1683
BspCNI CTCAG 5 cut(s) 18, 1252, 1608, 1768, 2193
BspEI TCCGGA 2 cut(s) 198, 643
BspFNI CGCG 2 cut(s) 933, 1953
BspLI GGNNCC 4 cut(s) 537, 951, 1380, 1822
BspMAI CTGCAG 1 cut(s) 2366
BspPI GGATC 3 cut(s) 119, 1910, 2283
BspT107I GGYRCC 1 cut(s) 535
BsrBI CCGCTC 1 cut(s) 707
BsrDI GCAATG 2 cut(s) 602, 2028
BsrFI RCCGGY 1 cut(s) 1491
BsrGI TGTACA 3 cut(s) 869, 1710, 2022
BsrI ACTGG 5 cut(s) 65, 953, 1925, 2374, 2557
BssAI RCCGGY 1 cut(s) 1491
BssECI CCNNGG 3 cut(s) 68, 69, 441
BssNI GRCGYC 1 cut(s) 144
BssSI CACGAG 1 cut(s) 312
Bst2BI CACGAG 1 cut(s) 312
Bst2UI CCWGG 1 cut(s) 1685
BstACI GRCGYC 1 cut(s) 144
BstAUI TGTACA 3 cut(s) 869, 1710, 2022
BstBAI YACGTR 2 cut(s) 40, 657
BstC8I GCNNGC 4 cut(s) 226, 896, 972, 1009
BstDEI CTNAG 7 cut(s) 5, 229, 1260, 1616, 1776, 2099, 2201
BstDSI CCRYGG 1 cut(s) 441
BstENI CCTNNNNNAGG 1 cut(s) 1263
BstF5I GGATG 4 cut(s) 652, 970, 1024, 1207
BstFNI CGCG 2 cut(s) 933, 1953
BstH2I RGCGCY 2 cut(s) 13, 1446
BstHHI GCGC 3 cut(s) 12, 1445, 2284
BstMAI GTCTC 1 cut(s) 315
BstMCI CGRYCG 1 cut(s) 109
BstMWI GCNNNNNNNGC 4 cut(s) 24, 2153, 2327, 2370
BstNI CCWGG 1 cut(s) 1685
BstNSI RCATGY 1 cut(s) 506
BstSCI CCNGG 3 cut(s) 68, 69, 1683
BstSFI CTRYAG 2 cut(s) 1210, 2362
BstUI CGCG 2 cut(s) 933, 1953
BstV1I GCAGC 3 cut(s) 1156, 2140, 2143
BstV2I GAAGAC 1 cut(s) 1997
BstX2I RGATCY 4 cut(s) 111, 1154, 1432, 1557
BstYI RGATCY 4 cut(s) 111, 1154, 1432, 1557
Bsu36I CCTNAGG 1 cut(s) 1260
BsuI GTATCC 2 cut(s) 1026, 2351
BsuRI GGCC 3 cut(s) 248, 271, 1683
BtgI CCRYGG 1 cut(s) 441
BtsCI GGATG 4 cut(s) 652, 970, 1024, 1207
BtsI GCAGTG 2 cut(s) 597, 2148
BtsIMutI CAGTG 6 cut(s) 58, 597, 872, 960, 1732, 2148
Cac8I GCNNGC 4 cut(s) 226, 896, 972, 1009
CfoI GCGC 3 cut(s) 12, 1445, 2284
Cfr10I RCCGGY 1 cut(s) 1491
Cfr13I GGNCC 1 cut(s) 270
Cfr9I CCCGGG 1 cut(s) 69
CseI GACGC 1 cut(s) 152
CviAII CATG 4 cut(s) 484, 503, 1037, 2144
DdeI CTNAG 7 cut(s) 5, 229, 1260, 1616, 1776, 2099, 2201
DraI TTTAAA 2 cut(s) 2392, 2398
EciI GGCGGA 1 cut(s) 725
Eco24I GRGCYC 1 cut(s) 1825
Eco57I CTGAAG 1 cut(s) 227
Eco72I CACGTG 1 cut(s) 40
Eco81I CCTNAGG 1 cut(s) 1260
Eco88I CYCGRG 1 cut(s) 69
EcoNI CCTNNNNNAGG 1 cut(s) 1263
EcoRI GAATTC 1 cut(s) 167
EcoRII CCWGG 1 cut(s) 1683
EcoT38I GRGCYC 1 cut(s) 1825
FaeI CATG 4 cut(s) 487, 506, 1040, 2147
FaqI GGGAC 2 cut(s) 86, 1806
FatI CATG 4 cut(s) 483, 502, 1036, 2143
FauI CCCGC 1 cut(s) 125
FauNDI CATATG 2 cut(s) 976, 1816
FbaI TGATCA 3 cut(s) 1092, 2454, 2514
Fnu4HI GCNGC 4 cut(s) 708, 1145, 2154, 2157
FokI GGATG 4 cut(s) 659, 977, 1031, 1214
FriOI GRGCYC 1 cut(s) 1825
Fsp4HI GCNGC 4 cut(s) 708, 1145, 2154, 2157
FspBI CTAG 5 cut(s) 1026, 1580, 1743, 2414, 2540
GlaI GCGC 3 cut(s) 11, 1444, 2283
GluI GCNGC 4 cut(s) 708, 1145, 2154, 2157
GsuI CTGGAG 3 cut(s) 2097, 2540, 2544
HaeII RGCGCY 2 cut(s) 13, 1446
HaeIII GGCC 3 cut(s) 248, 271, 1683
HapII CCGG 7 cut(s) 51, 70, 109, 199, 383, 644, 1492
HgaI GACGC 1 cut(s) 152
HhaI GCGC 3 cut(s) 12, 1445, 2284
Hin1I GRCGYC 1 cut(s) 144
Hin1II CATG 4 cut(s) 487, 506, 1040, 2147
Hin6I GCGC 3 cut(s) 10, 1443, 2282
HinP1I GCGC 3 cut(s) 10, 1443, 2282
HindIII AAGCTT 5 cut(s) 1109, 1241, 1653, 2385, 2498
HpaII CCGG 7 cut(s) 51, 70, 109, 199, 383, 644, 1492
HphI GGTGA 5 cut(s) 599, 682, 874, 1949, 2078
Hpy166II GTNNAC 3 cut(s) 429, 872, 2170
Hpy188I TCNGA 4 cut(s) 175, 838, 1224, 1661
Hpy8I GTNNAC 3 cut(s) 429, 872, 2170
Hpy99I CGWCG 2 cut(s) 33, 146
HpyCH4IV ACGT 2 cut(s) 39, 656
HpyF10VI GCNNNNNNNGC 4 cut(s) 24, 2153, 2327, 2370
HpyF3I CTNAG 7 cut(s) 5, 229, 1260, 1616, 1776, 2099, 2201
HpySE526I ACGT 2 cut(s) 39, 656
Hsp92I GRCGYC 1 cut(s) 144
Hsp92II CATG 4 cut(s) 487, 506, 1040, 2147
HspAI GCGC 3 cut(s) 10, 1443, 2282
Kpn2I TCCGGA 2 cut(s) 198, 643
Ksp22I TGATCA 3 cut(s) 1092, 2454, 2514
LmnI GCTCC 6 cut(s) 809, 949, 1384, 1576, 1820, 2326
Lsp1109I GCAGC 3 cut(s) 1156, 2140, 2143
LweI GCATC 5 cut(s) 1012, 1245, 1327, 1770, 2059
MaeI CTAG 5 cut(s) 1026, 1580, 1743, 2414, 2540
MaeII ACGT 2 cut(s) 39, 656
MaeIII GTNAC 9 cut(s) 498, 593, 757, 1184, 1205, 1285, 1810, 2094, 2332
MbiI CCGCTC 1 cut(s) 707
MboII GAAGA 7 cut(s) 1442, 1552, 1661, 1760, 1997, 2088, 2498
MfeI CAATTG 1 cut(s) 766
MflI RGATCY 4 cut(s) 111, 1154, 1432, 1557
MhlI GDGCHC 2 cut(s) 319, 1825
MlyI GAGTC 2 cut(s) 27, 48
MmeI TCCRAC 3 cut(s) 157, 198, 784
MroI TCCGGA 2 cut(s) 198, 643
MroXI GAANNNNTTC 1 cut(s) 1244
MseI TTAA 8 cut(s) 150, 849, 1166, 1358, 1515, 1665, 2391, 2397
MspI CCGG 7 cut(s) 51, 70, 109, 199, 383, 644, 1492
MspR9I CCNGG 3 cut(s) 70, 71, 1685
MunI CAATTG 1 cut(s) 766
Mva1269I GAATGC 1 cut(s) 327
MvaI CCWGG 1 cut(s) 1685
MvnI CGCG 2 cut(s) 933, 1953
MwoI GCNNNNNNNGC 4 cut(s) 24, 2153, 2327, 2370
NciI CCSGG 2 cut(s) 70, 71
NdeI CATATG 2 cut(s) 976, 1816
NlaIII CATG 4 cut(s) 487, 506, 1040, 2147
NlaIV GGNNCC 4 cut(s) 537, 951, 1380, 1822
NmeAIII GCCGAG 1 cut(s) 564
NmuCI GTSAC 2 cut(s) 593, 757
NspI RCATGY 1 cut(s) 506
PciI ACATGT 1 cut(s) 502
PctI GAATGC 1 cut(s) 327
PdmI GAANNNNTTC 1 cut(s) 1244
PkrI GCNGC 4 cut(s) 709, 1146, 2155, 2158
PleI GAGTC 2 cut(s) 27, 48
PmaCI CACGTG 1 cut(s) 40
PmlI CACGTG 1 cut(s) 40
PpsI GAGTC 2 cut(s) 27, 48
Ppu21I YACGTR 2 cut(s) 40, 657
PscI ACATGT 1 cut(s) 502
PshBI ATTAAT 1 cut(s) 1515
PsiI TTATAA 3 cut(s) 1293, 2246, 2469
Psp6I CCWGG 1 cut(s) 1683
PspCI CACGTG 1 cut(s) 40
PspGI CCWGG 1 cut(s) 1683
PspN4I GGNNCC 4 cut(s) 537, 951, 1380, 1822
PspPI GGNCC 1 cut(s) 270
PsrI GAACNNNNNNTAC 2 cut(s) 2359, 2391
PstI CTGCAG 1 cut(s) 2366
PsuI RGATCY 4 cut(s) 111, 1154, 1432, 1557
SaqAI TTAA 8 cut(s) 150, 849, 1166, 1358, 1515, 1665, 2391, 2397
SatI GCNGC 4 cut(s) 708, 1145, 2154, 2157
Sau96I GGNCC 1 cut(s) 270
ScaI AGTACT 1 cut(s) 1852
SchI GAGTC 2 cut(s) 27, 48
ScrFI CCNGG 3 cut(s) 70, 71, 1685
SduI GDGCHC 2 cut(s) 319, 1825
SfaNI GCATC 5 cut(s) 1012, 1245, 1327, 1770, 2059
SfcI CTRYAG 2 cut(s) 1210, 2362
SmaI CCCGGG 1 cut(s) 71
SmlI CTYRAG 3 cut(s) 278, 461, 1304
SmoI CTYRAG 3 cut(s) 278, 461, 1304
SsiI CCGC 8 cut(s) 25, 118, 528, 707, 710, 917, 933, 1550
SspI AATATT 1 cut(s) 1692
SspMI CTAG 5 cut(s) 1026, 1580, 1743, 2414, 2540
StyD4I CCNGG 3 cut(s) 68, 69, 1683
TaiI ACGT 2 cut(s) 42, 659
TaqI TCGA 9 cut(s) 105, 251, 319, 450, 662, 1248, 1563, 1700, 2278
TatI WGTACW 7 cut(s) 78, 869, 1710, 1850, 1906, 2022, 2551
TauI GCSGC 1 cut(s) 710
Tru1I TTAA 8 cut(s) 150, 849, 1166, 1358, 1515, 1665, 2391, 2397
Tru9I TTAA 8 cut(s) 150, 849, 1166, 1358, 1515, 1665, 2391, 2397
TscAI CASTG 6 cut(s) 65, 604, 879, 960, 1732, 2155
TseFI GTSAC 2 cut(s) 593, 757
TseI GCWGC 3 cut(s) 1144, 2153, 2156
Tsp45I GTSAC 2 cut(s) 593, 757
TspMI CCCGGG 1 cut(s) 69
TspRI CASTG 6 cut(s) 65, 604, 879, 960, 1732, 2155
VspI ATTAAT 1 cut(s) 1515
XagI CCTNNNNNAGG 1 cut(s) 1263
XapI RAATTY 5 cut(s) 167, 263, 1841, 1956, 2393
XbaI TCTAGA 2 cut(s) 1742, 2413
XceI RCATGY 1 cut(s) 506
XmaI CCCGGG 1 cut(s) 69
XmnI GAANNNNTTC 1 cut(s) 1244
XspI CTAG 5 cut(s) 1026, 1580, 1743, 2414, 2540
ZrmI AGTACT 1 cut(s) 1852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.