MD16G1004600.v1.1

Prolyl endopeptidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011215
Physical Location & Seq
Reverse (-)
346259 .. 352921
6663 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1004600.v1.1.491

Sequence Viewer

Length: 2340 bp
ATGGGAATGGTAATGAGAATGCATAAGCAGTGGCAGTGGCGCAGAGGGAACCTACTTCCGCTTGTCTACCTTCCAGTAGTCGTATTGTCGTCATTTGGTGGTGGCGCCGGTTCCTCTCTAGGTTTCTCCGTCCGAGCGTCCACGTCATCAGAACCCACATCAAGAGTGAGGATGAGTGAGAGTGAGATGCAGCGGCCTCCGGTGGCGAAGAAGGTGGAGCATAAGATGGAGATGTTCGGCGACGTCCGCGTGGACAACTACTATTGGCTCCGCGACGACTCTCGCTCCGACCCCCAAGTCCTCTCTTACCTTCAACAAGAAAACGATTATACTCAATTCCTCATGTCTGGAAATAAGAAATTCGAAGATCAACTCTACTCTGAGATTAGAGGGAGGATCAAGGAGGATGATATATCTGCACCTGAACGAAAAGGCCCCTACTATTACTATCAAAGGACTCTCAACGGTATGGAGTACGTTCAGCATTGTCGCCGCTTAATACCCAAAACCAGCAGCGAGGGTGATGCGGACCCCCCAGTATCTGTGTATGATACCATGCCCACCGGACCTGATGCTCCTCCGGAGCATGTAATCTTCGATGAGAATGTTATGGCTAAAAATCATGCCTATTATAGCATTGCTGCTTTTAAGGTTAGTCCAAACAACAATTTGGTGGCATATGCAGAAGACACCAAAGGAAATGAAATCTATACAGTTTATGTCATTGATGCCGAGACAGGTGCTCCTGTGGGTCTGCCTCTTGTTGGTACGACATCCTATCTTGAATGGGCCGGCAACGAGGCTTTAGTTTACATTACAATGGACGAGACCCTCCGCCCTGACAAGGCATGGTTACATAAGTTGGGGACAGAACAGTCAAGTGACTCCTGCCTCTATCACGAACTGGATGATATGTTTTCAATTGATCTTCAAGCTTCTGAGAGCAAAAAGTTCTTGTTTGTTGCATCCGAAAGTAAAACTACAAGATTCAACTTTTACCTTGATAATTCAAAGCCTGAAGATGGGCTTATGGTTCTGACACCTCGCCTAGATGGCATTGATACATTTGTTAGCCATCGAGGAAATCATTTTTTTATAAAGAGGAGGAGCAACCAGTTTTTTAATTCTGAAGTAATTGCTTGTCCATTGGAAAATACATCTGAAACTACAGTTCTTCTTCCACACAGGGAAAGTGTGAAAATTCAGGATATACATCTTTTTAGTGATCACCTTGTTGTATATGAACGTGAAGAGGGTCTACCAAAAGTCACTATCTATCAACTTCCAGATGTTGGACAACCACTTAAAACCCTTCATGGTGGTCAAGCTGTTAAATTTTTGGATCCCACATACTCTGTGGATCCATCAGAATCAGAATTTTCTTCCTGCATCTTACGATTTTCTTATAGCTCCATGAAAACACCTCCCTCTGTGTATGATTATGATATGAAGACAGGTGTTTCTGTTTTGAAGAAGATTGAAACGGTTTTGGGAGGTTTTGAAGCATCAGATTATGTTACAGAAAGGAAATGGGCTAGTGCTCCAGATGGTACTCAGATTCCAATATCAATTGTTTATAGGAAGGATATTGTAAAACTTGATGGATCTGATCCATTGCTACTCTATGGCTATGGCTCTTATGAGGTCTGTATAGATGCCAGCTTTAAAGCTTCAAGGCTCTCTTTGCTGGATAGGGGTTTTATATATGCTATAGCTCATATTCGTGGGGGTGGTGAAATGGGGAGGCAGTGGTACGAGAATGGAAAGTTGCTGAAGAAGAAAAATACTTTTACAGATTTTATTGCTTGTGCTGAGTATTTGATAGAAAAGAAATATTGCTACAAAGAAAGATTATGCATTGATGGAAGGAGTGCAGGTGGATTGCTTATTGGTGCTGTTCTTAATATGAGGCCTGATTTGTTCAAGGCTGCTGTTGCTGGAGTGCCTTTTGTTGATGTTCTGACTACAATGCTTGACCCAACTATCCCCCTTACAACTTCAGAGTGGGAGGAATGGGGTGACCCACGTAAAGAGGAGTTCTACTTTTACATGAAGTCATATTCCCCTGTTGATAACGTTAAGGCTCAAAATTATCCGAACATTCTTGTTACTGCTGGTCTAAATGATCCACGGGTTATGTACTCAGAACCTGCTAAATTTGTGGCAAATCTAAGGGATAAGAAGACTGATAAAAACATCATTATGTTTAAATGTGAACTTGGTGCTGGGCATTTTTCAAAGTCAGGGAGGTTTGAGAAGCTTCAAGAAGATGCCTTCATATATACCTTCATAATGAAGACTCTAGATATGGTTCCTGCCGGAGGGTCCGGGGGGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05142 ko05143 map05142 map05143
Pfam Domains
Protein Families
Peptidase_S9 Peptidase_S9_N

Protein Analysis

780

Amino Acids

88.16

Weight (kDa)

5.57

Isoelectric Point (pI)

42.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S9_N PF02897 66 - 491 9.1e-104 Prolyl oligopeptidase, N-terminal beta-propeller domain
Peptidase_S9 PF00326 554 - 769 3e-66 Prolyl oligopeptidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011215)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50380 AT1G50380
fragaria_vesca FvH4_4g37301
malus_domestica MD16G1004600.v1.1
prunus_persica Prupe.1G351000_v2.0.a1 Prupe.1G351200_v2.0.a1 Prupe.1G351200_v2.0.a1
pyrus_communis pycom16g00020 pycom16g00030
rosa_chinensis RchiOBHm_Chr4g0447131 RchiOBHm_Chr5g0037361
rosa_laevigata RLG00000005602
rosa_multiflora Rmu_ssc0000178.1_g000010
rosa_roxburghii Rroxscaffold_5G00387470
rosa_rugosa Rorug04G0378500
rosa_samantha Rh4AG439600 Rh4BG448700 Rh4CG465700 Rh4DG447100
rosa_wichuraiana Rw4G037610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1097
AarI CACCTGC 1 cut(s) 1865
AasI GACNNNNNNGTC 2 cut(s) 296, 874
AatII GACGTC 1 cut(s) 246
Acc36I ACCTGC 2 cut(s) 1865, 2158
AccB1I GGYRCC 1 cut(s) 104
AccB7I CCANNNNNTGG 1 cut(s) 1292
AccI GTMKAC 2 cut(s) 66, 1258
AccII CGCG 2 cut(s) 249, 273
AccIII TCCGGA 1 cut(s) 580
AciI CCGC 7 cut(s) 59, 193, 247, 271, 493, 527, 835
AclI AACGTT 1 cut(s) 2076
AclWI GGATC 8 cut(s) 404, 1337, 1350, 1355, 1368, 1604, 1612, 2120
AcsI RAATTY 5 cut(s) 359, 1200, 1334, 1376, 2156
AcuI CTGAAG 4 cut(s) 1038, 1149, 1793, 1983
AcyI GRCGYC 2 cut(s) 105, 243
AfaI GTAC 5 cut(s) 476, 769, 1552, 1754, 2141
AfiI CCNNNNNNNGG 5 cut(s) 764, 844, 1022, 1292, 2321
AjiI CACGTC 1 cut(s) 144
AluBI AGCT 7 cut(s) 935, 1328, 1410, 1662, 1670, 1715, 2260
AluI AGCT 7 cut(s) 935, 1328, 1410, 1662, 1670, 1715, 2260
Alw21I GWGCWC 2 cut(s) 745, 1543
Alw26I GTCTC 2 cut(s) 728, 821
AlwI GGATC 8 cut(s) 404, 1337, 1350, 1355, 1368, 1604, 1612, 2120
AlwNI CAGNNNCTG 2 cut(s) 542, 2150
Aor13HI TCCGGA 1 cut(s) 580
AoxI GGCC 4 cut(s) 194, 433, 789, 1910
ApeKI GCWGC 4 cut(s) 190, 513, 641, 1928
ApoI RAATTY 5 cut(s) 359, 1200, 1334, 1376, 2156
AspLEI GCGC 2 cut(s) 42, 107
AspS9I GGNCC 5 cut(s) 434, 529, 566, 789, 2325
AsuC2I CCSGG 1 cut(s) 2329
AsuHPI GGTGA 4 cut(s) 533, 1220, 1745, 2030
AsuII TTCGAA 1 cut(s) 363
AvaII GGWCC 3 cut(s) 529, 566, 2325
BaeI ACNNNNGTAYC 2 cut(s) 1053, 1086
BamHI GGATCC 2 cut(s) 1342, 1360
BanI GGYRCC 1 cut(s) 104
BarI GAAGNNNNNNTAC 2 cut(s) 1242, 1274
BbsI GAAGAC 4 cut(s) 693, 1457, 2189, 2303
Bbv12I GWGCWC 2 cut(s) 745, 1543
BbvI GCAGC 4 cut(s) 202, 525, 628, 1915
BccI CCATC 8 cut(s) 220, 1016, 1046, 1083, 1372, 1541, 1595, 1856
BcgI CGANNNNNNTGC 4 cut(s) 506, 540, 722, 756
BclI TGATCA 1 cut(s) 1225
BcnI CCSGG 1 cut(s) 2329
BcoDI GTCTC 2 cut(s) 728, 821
BfaI CTAG 4 cut(s) 119, 1049, 1536, 2303
BfmI CTRYAG 2 cut(s) 1167, 1710
BfoI RGCGCY 1 cut(s) 108
BfuAI ACCTGC 2 cut(s) 1865, 2158
BglI GCCNNNNNGGC 1 cut(s) 1053
BisI GCNGC 6 cut(s) 191, 194, 493, 514, 642, 1929
BlsI GCNGC 6 cut(s) 192, 195, 494, 515, 643, 1930
Bme1390I CCNGG 1 cut(s) 2329
Bme18I GGWCC 3 cut(s) 529, 566, 2325
BmgBI CACGTC 1 cut(s) 144
BmgT120I GGNCC 5 cut(s) 434, 529, 566, 789, 2325
BmrFI CCNGG 1 cut(s) 2329
BmrI ACTGGG 1 cut(s) 530
BmsI GCATC 9 cut(s) 177, 514, 562, 718, 974, 1398, 1514, 1645, 2260
BmuI ACTGGG 1 cut(s) 530
BpiI GAAGAC 4 cut(s) 693, 1457, 2189, 2303
BpmI CTGGAG 2 cut(s) 1527, 1959
Bpu14I TTCGAA 1 cut(s) 363
BpuMI CCSGG 1 cut(s) 2329
BsaAI YACGTR 1 cut(s) 2027
BsaBI GATNNNNATC 1 cut(s) 1212
BsaHI GRCGYC 2 cut(s) 105, 243
BsaI GGTCTC 1 cut(s) 821
BsaJI CCNNGG 2 cut(s) 2129, 2328
BsaWI WCCGGW 3 cut(s) 199, 563, 580
BsaXI ACNNNNNCTCC 6 cut(s) 269, 299, 727, 757, 1861, 1891
Bsc4I CCNNNNNNNGG 5 cut(s) 764, 844, 1022, 1292, 2321
Bse118I RCCGGY 2 cut(s) 107, 791
Bse1I ACTGG 4 cut(s) 74, 536, 909, 1114
Bse3DI GCAATG 2 cut(s) 636, 1613
Bse8I GATNNNNATC 1 cut(s) 1212
BseAI TCCGGA 1 cut(s) 580
BseDI CCNNGG 2 cut(s) 2129, 2328
BseGI GGATG 5 cut(s) 177, 412, 773, 913, 965
BseJI GATNNNNATC 1 cut(s) 1212
BseLI CCNNNNNNNGG 5 cut(s) 764, 844, 1022, 1292, 2321
BseMI GCAATG 2 cut(s) 636, 1613
BseMII CTCAG 5 cut(s) 372, 930, 1568, 1803, 2157
BseNI ACTGG 4 cut(s) 74, 536, 909, 1114
BseRI GAGGAG 4 cut(s) 567, 1117, 1120, 2048
BseXI GCAGC 4 cut(s) 202, 525, 628, 1915
BseYI CCCAGC 1 cut(s) 2225
BsgI GTGCAG 2 cut(s) 402, 1893
Bsh1236I CGCG 2 cut(s) 249, 273
BshFI GGCC 4 cut(s) 196, 435, 791, 1912
BshNI GGYRCC 1 cut(s) 104
BsiHKAI GWGCWC 2 cut(s) 745, 1543
BsiSI CCGG 7 cut(s) 108, 200, 564, 581, 792, 2319, 2328
BslFI GGGAC 1 cut(s) 880
BslI CCNNNNNNNGG 5 cut(s) 764, 844, 1022, 1292, 2321
BsmAI GTCTC 2 cut(s) 728, 821
BsmFI GGGAC 1 cut(s) 880
BsmI GAATGC 1 cut(s) 24
BsnI GGCC 4 cut(s) 196, 435, 791, 1912
Bso31I GGTCTC 1 cut(s) 821
Bsp119I TTCGAA 1 cut(s) 363
Bsp1286I GDGCHC 2 cut(s) 745, 1543
Bsp13I TCCGGA 1 cut(s) 580
Bsp143I GATC 9 cut(s) 367, 396, 925, 1225, 1342, 1360, 1604, 1609, 2125
BspACI CCGC 7 cut(s) 59, 193, 247, 271, 493, 527, 835
BspANI GGCC 4 cut(s) 196, 435, 791, 1912
BspCNI CTCAG 5 cut(s) 373, 931, 1567, 1804, 2156
BspEI TCCGGA 1 cut(s) 580
BspFNI CGCG 2 cut(s) 249, 273
BspMI ACCTGC 2 cut(s) 1865, 2158
BspPI GGATC 8 cut(s) 404, 1337, 1350, 1355, 1368, 1604, 1612, 2120
BspT104I TTCGAA 1 cut(s) 363
BspT107I GGYRCC 1 cut(s) 104
BspTNI GGTCTC 1 cut(s) 821
BsrDI GCAATG 2 cut(s) 636, 1613
BsrFI RCCGGY 2 cut(s) 107, 791
BsrI ACTGG 4 cut(s) 74, 536, 909, 1114
BssAI RCCGGY 2 cut(s) 107, 791
BssECI CCNNGG 2 cut(s) 2129, 2328
BssMI GATC 9 cut(s) 367, 396, 925, 1225, 1342, 1360, 1604, 1609, 2125
BssNI GRCGYC 2 cut(s) 105, 243
Bst4CI ACNGT 5 cut(s) 467, 715, 876, 1171, 1486
Bst6I CTCTTC 1 cut(s) 1245
BstACI GRCGYC 2 cut(s) 105, 243
BstBAI YACGTR 1 cut(s) 2027
BstBI TTCGAA 1 cut(s) 363
BstC8I GCNNGC 2 cut(s) 793, 1660
BstDEI CTNAG 6 cut(s) 381, 939, 1554, 1812, 2143, 2171
BstDSI CCRYGG 1 cut(s) 2129
BstEII GGTNACC 1 cut(s) 2018
BstENI CCTNNNNNAGG 1 cut(s) 2319
BstF5I GGATG 5 cut(s) 177, 412, 773, 913, 965
BstFNI CGCG 2 cut(s) 249, 273
BstH2I RGCGCY 1 cut(s) 108
BstHHI GCGC 2 cut(s) 42, 107
BstKTI GATC 9 cut(s) 370, 399, 928, 1228, 1345, 1363, 1607, 1612, 2128
BstMAI GTCTC 2 cut(s) 728, 821
BstMBI GATC 9 cut(s) 367, 396, 925, 1225, 1342, 1360, 1604, 1609, 2125
BstMWI GCNNNNNNNGC 4 cut(s) 246, 1053, 1684, 1934
BstNSI RCATGY 1 cut(s) 590
BstPI GGTNACC 1 cut(s) 2018
BstSCI CCNGG 1 cut(s) 2327
BstSFI CTRYAG 2 cut(s) 1167, 1710
BstUI CGCG 2 cut(s) 249, 273
BstV1I GCAGC 4 cut(s) 202, 525, 628, 1915
BstV2I GAAGAC 4 cut(s) 693, 1457, 2189, 2303
BstX2I RGATCY 3 cut(s) 1342, 1360, 1604
BstYI RGATCY 3 cut(s) 1342, 1360, 1604
BsuRI GGCC 4 cut(s) 196, 435, 791, 1912
BtgI CCRYGG 1 cut(s) 2129
BtrI CACGTC 1 cut(s) 144
BtsCI GGATG 5 cut(s) 177, 412, 773, 913, 965
BtsI GCAGTG 3 cut(s) 35, 41, 1754
BtsIMutI CAGTG 3 cut(s) 35, 41, 1754
BveI ACCTGC 2 cut(s) 1865, 2158
Cac8I GCNNGC 2 cut(s) 793, 1660
CaiI CAGNNNCTG 2 cut(s) 542, 2150
CfoI GCGC 2 cut(s) 42, 107
Cfr10I RCCGGY 2 cut(s) 107, 791
Cfr13I GGNCC 5 cut(s) 434, 529, 566, 789, 2325
CseI GACGC 1 cut(s) 126
Csp6I GTAC 5 cut(s) 475, 768, 1551, 1753, 2140
CviAII CATG 8 cut(s) 343, 556, 587, 623, 849, 1316, 1414, 2050
CviQI GTAC 5 cut(s) 475, 768, 1551, 1753, 2140
DdeI CTNAG 6 cut(s) 381, 939, 1554, 1812, 2143, 2171
DinI GGCGCC 1 cut(s) 106
DpnI GATC 9 cut(s) 369, 398, 927, 1227, 1344, 1362, 1606, 1611, 2127
DpnII GATC 9 cut(s) 367, 396, 925, 1225, 1342, 1360, 1604, 1609, 2125
DraI TTTAAA 2 cut(s) 1666, 2209
DrdI GACNNNNNNGTC 2 cut(s) 296, 874
DseDI GACNNNNNNGTC 2 cut(s) 296, 874
Eam1104I CTCTTC 1 cut(s) 1245
EarI CTCTTC 1 cut(s) 1245
EciI GGCGGA 1 cut(s) 824
Eco147I AGGCCT 1 cut(s) 1912
Eco31I GGTCTC 1 cut(s) 821
Eco47I GGWCC 3 cut(s) 529, 566, 2325
Eco57I CTGAAG 4 cut(s) 1038, 1149, 1793, 1983
Eco91I GGTNACC 1 cut(s) 2018
EcoNI CCTNNNNNAGG 1 cut(s) 2319
EcoO109I RGGNCCY 1 cut(s) 434
EcoO65I GGTNACC 1 cut(s) 2018
EcoT22I ATGCAT 2 cut(s) 24, 1859
EgeI GGCGCC 1 cut(s) 106
EheI GGCGCC 1 cut(s) 106
FaeI CATG 8 cut(s) 346, 559, 590, 626, 852, 1319, 1417, 2053
FalI AAGNNNNNCTT 4 cut(s) 1011, 1043, 1666, 1698
FaqI GGGAC 1 cut(s) 880
FatI CATG 8 cut(s) 342, 555, 586, 622, 848, 1315, 1413, 2049
FauNDI CATATG 1 cut(s) 679
FbaI TGATCA 1 cut(s) 1225
FblI GTMKAC 2 cut(s) 66, 1258
Fnu4HI GCNGC 6 cut(s) 191, 194, 493, 514, 642, 1929
FokI GGATG 5 cut(s) 184, 419, 760, 920, 952
Fsp4HI GCNGC 6 cut(s) 191, 194, 493, 514, 642, 1929
FspBI CTAG 4 cut(s) 119, 1049, 1536, 2303
GlaI GCGC 2 cut(s) 41, 106
GluI GCNGC 6 cut(s) 191, 194, 493, 514, 642, 1929
GsaI CCCAGC 1 cut(s) 2229
GsuI CTGGAG 2 cut(s) 1527, 1959
HaeII RGCGCY 1 cut(s) 108
HaeIII GGCC 4 cut(s) 196, 435, 791, 1912
HapII CCGG 7 cut(s) 108, 200, 564, 581, 792, 2319, 2328
HgaI GACGC 1 cut(s) 126
HhaI GCGC 2 cut(s) 42, 107
Hin1I GRCGYC 2 cut(s) 105, 243
Hin1II CATG 8 cut(s) 346, 559, 590, 626, 852, 1319, 1417, 2053
Hin6I GCGC 2 cut(s) 40, 105
HinP1I GCGC 2 cut(s) 40, 105
HindIII AAGCTT 3 cut(s) 933, 1668, 2258
HinfI GANTC 7 cut(s) 278, 457, 884, 987, 1370, 1558, 2299
HpaII CCGG 7 cut(s) 108, 200, 564, 581, 792, 2319, 2328
HphI GGTGA 4 cut(s) 533, 1220, 1745, 2030
Hpy166II GTNNAC 6 cut(s) 67, 141, 253, 811, 1259, 2216
Hpy8I GTNNAC 6 cut(s) 67, 141, 253, 811, 1259, 2216
Hpy99I CGWCG 2 cut(s) 245, 278
HpyAV CCTTC 8 cut(s) 80, 205, 320, 1322, 1576, 1860, 2284, 2296
HpyCH4III ACNGT 5 cut(s) 467, 715, 876, 1171, 1486
HpyCH4IV ACGT 6 cut(s) 143, 243, 477, 1246, 2026, 2076
HpyCH4V TGCA 8 cut(s) 22, 190, 419, 683, 965, 1389, 1857, 1874
HpyF10VI GCNNNNNNNGC 4 cut(s) 246, 1053, 1684, 1934
HpyF3I CTNAG 6 cut(s) 381, 939, 1554, 1812, 2143, 2171
HpySE526I ACGT 6 cut(s) 143, 243, 477, 1246, 2026, 2076
Hsp92I GRCGYC 2 cut(s) 105, 243
Hsp92II CATG 8 cut(s) 346, 559, 590, 626, 852, 1319, 1417, 2053
HspAI GCGC 2 cut(s) 40, 105
KasI GGCGCC 1 cut(s) 104
Kpn2I TCCGGA 1 cut(s) 580
KroI GCCGGC 1 cut(s) 791
KroNI GCCGGC 1 cut(s) 793
Ksp22I TGATCA 1 cut(s) 1225
Kzo9I GATC 9 cut(s) 367, 396, 925, 1225, 1342, 1360, 1604, 1609, 2125
LmnI GCTCC 9 cut(s) 217, 273, 290, 580, 583, 748, 1107, 1415, 1546
Lsp1109I GCAGC 4 cut(s) 202, 525, 628, 1915
LweI GCATC 9 cut(s) 177, 514, 562, 718, 974, 1398, 1514, 1645, 2260
MaeI CTAG 4 cut(s) 119, 1049, 1536, 2303
MaeII ACGT 6 cut(s) 143, 243, 477, 1246, 2026, 2076
MaeIII GTNAC 6 cut(s) 852, 881, 1267, 1516, 2018, 2107
MalI GATC 9 cut(s) 369, 398, 927, 1227, 1344, 1362, 1606, 1611, 2127
MboI GATC 9 cut(s) 367, 396, 925, 1225, 1342, 1360, 1604, 1609, 2125
MfeI CAATTG 2 cut(s) 921, 1569
MflI RGATCY 3 cut(s) 1342, 1360, 1604
MhlI GDGCHC 2 cut(s) 745, 1543
Mly113I GGCGCC 1 cut(s) 105
MlyI GAGTC 4 cut(s) 272, 451, 878, 2293
MmeI TCCRAC 2 cut(s) 312, 1273
Mph1103I ATGCAT 2 cut(s) 24, 1859
MroI TCCGGA 1 cut(s) 580
MroNI GCCGGC 1 cut(s) 791
MslI CAYNNNNRTG 3 cut(s) 818, 1722, 2201
MspA1I CMGCKG 1 cut(s) 193
MspI CCGG 7 cut(s) 108, 200, 564, 581, 792, 2319, 2328
MspR9I CCNGG 1 cut(s) 2329
MunI CAATTG 2 cut(s) 921, 1569
Mva1269I GAATGC 1 cut(s) 24
MvnI CGCG 2 cut(s) 249, 273
MwoI GCNNNNNNNGC 4 cut(s) 246, 1053, 1684, 1934
NaeI GCCGGC 1 cut(s) 793
NarI GGCGCC 1 cut(s) 105
NciI CCSGG 1 cut(s) 2329
NdeI CATATG 1 cut(s) 679
NdeII GATC 9 cut(s) 367, 396, 925, 1225, 1342, 1360, 1604, 1609, 2125
NgoMIV GCCGGC 1 cut(s) 791
NlaIII CATG 8 cut(s) 346, 559, 590, 626, 852, 1319, 1417, 2053
NmeAIII GCCGAG 1 cut(s) 757
NmuCI GTSAC 3 cut(s) 881, 1267, 2018
NsiI ATGCAT 2 cut(s) 24, 1859
NspI RCATGY 1 cut(s) 590
NspV TTCGAA 1 cut(s) 363
PaqCI CACCTGC 1 cut(s) 1865
PceI AGGCCT 1 cut(s) 1912
PctI GAATGC 1 cut(s) 24
PdiI GCCGGC 1 cut(s) 793
PfeI GAWTC 3 cut(s) 987, 1370, 1558
PflMI CCANNNNNTGG 1 cut(s) 1292
PkrI GCNGC 6 cut(s) 192, 195, 494, 515, 643, 1930
PleI GAGTC 4 cut(s) 272, 451, 878, 2293
PluTI GGCGCC 1 cut(s) 108
PpsI GAGTC 4 cut(s) 272, 451, 878, 2293
Ppu21I YACGTR 1 cut(s) 2027
PsiI TTATAA 1 cut(s) 1097
Psp1406I AACGTT 1 cut(s) 2076
PspEI GGTNACC 1 cut(s) 2018
PspFI CCCAGC 1 cut(s) 2225
PspPI GGNCC 5 cut(s) 434, 529, 566, 789, 2325
PstNI CAGNNNCTG 2 cut(s) 542, 2150
PsuI RGATCY 3 cut(s) 1342, 1360, 1604
RsaI GTAC 5 cut(s) 476, 769, 1552, 1754, 2141
RsaNI GTAC 5 cut(s) 475, 768, 1551, 1753, 2140
RseI CAYNNNNRTG 3 cut(s) 818, 1722, 2201
SatI GCNGC 6 cut(s) 191, 194, 493, 514, 642, 1929
Sau3AI GATC 9 cut(s) 367, 396, 925, 1225, 1342, 1360, 1604, 1609, 2125
Sau96I GGNCC 5 cut(s) 434, 529, 566, 789, 2325
SchI GAGTC 4 cut(s) 272, 451, 878, 2293
ScrFI CCNGG 1 cut(s) 2329
SduI GDGCHC 2 cut(s) 745, 1543
SfaNI GCATC 9 cut(s) 177, 514, 562, 718, 974, 1398, 1514, 1645, 2260
SfcI CTRYAG 2 cut(s) 1167, 1710
SfoI GGCGCC 1 cut(s) 106
SfuI TTCGAA 1 cut(s) 363
SinI GGWCC 3 cut(s) 529, 566, 2325
SmiMI CAYNNNNRTG 3 cut(s) 818, 1722, 2201
SseBI AGGCCT 1 cut(s) 1912
SsiI CCGC 7 cut(s) 59, 193, 247, 271, 493, 527, 835
SspDI GGCGCC 1 cut(s) 104
SspI AATATT 1 cut(s) 1835
SspMI CTAG 4 cut(s) 119, 1049, 1536, 2303
StuI AGGCCT 1 cut(s) 1912
StyD4I CCNGG 1 cut(s) 2327
TaaI ACNGT 5 cut(s) 467, 715, 876, 1171, 1486
TaiI ACGT 6 cut(s) 146, 246, 480, 1249, 2029, 2079
TaqI TCGA 3 cut(s) 363, 597, 1078
TatI WGTACW 1 cut(s) 2139
TauI GCSGC 2 cut(s) 196, 495
TfiI GAWTC 3 cut(s) 987, 1370, 1558
TscAI CASTG 3 cut(s) 35, 41, 1754
TseFI GTSAC 3 cut(s) 881, 1267, 2018
TseI GCWGC 4 cut(s) 190, 513, 641, 1928
Tsp45I GTSAC 3 cut(s) 881, 1267, 2018
TspDTI ATGAA 9 cut(s) 717, 1257, 1304, 1430, 1463, 2066, 2266, 2278, 2309
TspGWI ACGGA 1 cut(s) 118
TspRI CASTG 3 cut(s) 35, 41, 1754
Van91I CCANNNNNTGG 1 cut(s) 1292
VpaK11BI GGWCC 3 cut(s) 529, 566, 2325
XagI CCTNNNNNAGG 1 cut(s) 2319
XapI RAATTY 5 cut(s) 359, 1200, 1334, 1376, 2156
XbaI TCTAGA 1 cut(s) 2302
XceI RCATGY 1 cut(s) 590
XcmI CCANNNNNNNNNTGG 1 cut(s) 1354
XmiI GTMKAC 2 cut(s) 66, 1258
XspI CTAG 4 cut(s) 119, 1049, 1536, 2303
ZraI GACGTC 1 cut(s) 244
Zsp2I ATGCAT 2 cut(s) 24, 1859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.