pycom16g00030

Prolyl endopeptidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0011215
Physical Location & Seq
Forward (+)
15896 .. 19423
3528 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g00030.4

Sequence Viewer

Length: 1101 bp
ATGAGCGTGAAGAGGGTCTACCAAAAGTCACTATCTATCAACTTCCAGATGTTGGACAACCACTTAAAACCCTTCATGGTGGTCAAGCTGTTAAATTTTTGGATCCCACATACTCTGATGGATCCATCAGAATCAGAATTTTCTTCCAGCATCTTACGATTTTCTTATAGCTCCATGAAAACACCTCCCTCTGTGTACGATTATGATATGAAGACAGGTGTTTCTGTTTTGAAGAAGATTGAAACAGTTTTGGGAGGTTTTGAAGCATCAGATTATGTTACAGAAAGGAAATGGGCTAGTGCTCTAGATGGTACTCAGATTCCAATATCAATTGTTTATAGGAAGGATATTGTAAAACTTGATGGATATGATCCATTGCTACTCTACGGCTATGGCTCTTATGAGATCTGTATAGATGCCAGCTTTAAAGCTTCAAGGCTGTCTTTGCTGGATAGGGGTTTTATATATGCTATAGCTCATATTCGTGGGGGTGGTGAAATGGGGAGGCAGTGGTACGAGAATGGAAAGTTGCTGAAGAAGAAAAATACGTTTACAGATTTTATTGCTTGTGCTGAGTATTTGATAGAAAAGAAATATTGCTCCAAAGAAAGATTATGCATTGATGGAAGGAGTGCAGGGGGATTGCTTATTGGTGCTGTTCTTAATATGAGGCCTGATTTGTTCAAGGCTGCTGTTGCTGGAGTGCCTTTTGTTGATGTTCTGACTACAATGCTTGACCCAACTATCCCCCTTACAACTTCAGAGTGGGAGGAATGGGGTGACCCACGTAAAGAGGAGTTCTACTTTTACATGAAGTCATATTCCCCTGTTGACAACGTTAAGGCTCAAAATTATCCGAACATTCTTGTTACTGCTGGTCTAAATGATCCACGGGTTATGTACTCAGAACCTGCTAAATTTGTGGCAAATCTAAGGGATAAGAAGACTGATAAAAACATCATTATGTTTAAATGTGAACTTGGTGCTGGGCATTTTTCAAAGTCAGGGAGGTTTGAGAAGCTTCAAGAAGATGCCTTCATATATACCTTCATAATGAAGACTCTAGATATGGTTCCTGCCGGAGGGTCCGGGGGGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05142 ko05143 map05142 map05143
Pfam Domains
Protein Families
Peptidase_S9 Peptidase_S9_N

Protein Analysis

367

Amino Acids

41.41

Weight (kDa)

8.36

Isoelectric Point (pI)

33.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011215)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50380 AT1G50380
fragaria_vesca FvH4_4g37301
malus_domestica MD16G1004600.v1.1
prunus_persica Prupe.1G351000_v2.0.a1 Prupe.1G351200_v2.0.a1 Prupe.1G351200_v2.0.a1
pyrus_communis pycom16g00020 pycom16g00030
rosa_chinensis RchiOBHm_Chr4g0447131 RchiOBHm_Chr5g0037361
rosa_laevigata RLG00000005602
rosa_multiflora Rmu_ssc0000178.1_g000010
rosa_roxburghii Rroxscaffold_5G00387470
rosa_rugosa Rorug04G0378500
rosa_samantha Rh4AG439600 Rh4BG448700 Rh4CG465700 Rh4DG447100
rosa_wichuraiana Rw4G037610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 919
AccB7I CCANNNNNTGG 1 cut(s) 52
AccI GTMKAC 1 cut(s) 18
AclI AACGTT 1 cut(s) 837
AclWI GGATC 6 cut(s) 97, 110, 116, 129, 365, 881
AcsI RAATTY 3 cut(s) 94, 137, 917
AcuI CTGAAG 2 cut(s) 554, 744
AfaI GTAC 4 cut(s) 197, 313, 515, 902
AfiI CCNNNNNNNGG 2 cut(s) 52, 1082
AgsI TTSAA 7 cut(s) 232, 242, 263, 435, 685, 999, 1025
AluBI AGCT 6 cut(s) 88, 171, 423, 431, 476, 1021
AluI AGCT 6 cut(s) 88, 171, 423, 431, 476, 1021
Alw21I GWGCWC 1 cut(s) 304
AlwI GGATC 6 cut(s) 97, 110, 116, 129, 365, 881
AlwNI CAGNNNCTG 1 cut(s) 911
AoxI GGCC 1 cut(s) 671
ApeKI GCWGC 1 cut(s) 689
ApoI RAATTY 3 cut(s) 94, 137, 917
AspS9I GGNCC 1 cut(s) 1086
AsuC2I CCSGG 1 cut(s) 1090
AsuHPI GGTGA 2 cut(s) 506, 791
AvaII GGWCC 1 cut(s) 1086
BamHI GGATCC 2 cut(s) 102, 121
BarI GAAGNNNNNNTAC 1 cut(s) 34
BbsI GAAGAC 3 cut(s) 218, 950, 1064
Bbv12I GWGCWC 1 cut(s) 304
BbvI GCAGC 1 cut(s) 676
BccI CCATC 5 cut(s) 112, 133, 302, 356, 617
BceAI ACGGC 1 cut(s) 403
BcnI CCSGG 1 cut(s) 1090
BfaI CTAG 3 cut(s) 297, 305, 1064
BfmI CTRYAG 1 cut(s) 471
BfuAI ACCTGC 1 cut(s) 919
BglII AGATCT 1 cut(s) 405
BisI GCNGC 1 cut(s) 690
BlsI GCNGC 1 cut(s) 691
Bme1390I CCNGG 1 cut(s) 1090
Bme18I GGWCC 1 cut(s) 1086
BmgT120I GGNCC 1 cut(s) 1086
BmiI GGNNCC 4 cut(s) 104, 123, 1074, 1087
BmrFI CCNGG 1 cut(s) 1090
BmsI GCATC 4 cut(s) 159, 275, 406, 1021
BpiI GAAGAC 3 cut(s) 218, 950, 1064
BpmI CTGGAG 1 cut(s) 720
BpuMI CCSGG 1 cut(s) 1090
BsaAI YACGTR 1 cut(s) 788
BsaJI CCNNGG 2 cut(s) 890, 1089
Bsc4I CCNNNNNNNGG 2 cut(s) 52, 1082
Bse3DI GCAATG 1 cut(s) 374
BseDI CCNNGG 2 cut(s) 890, 1089
BseLI CCNNNNNNNGG 2 cut(s) 52, 1082
BseMI GCAATG 1 cut(s) 374
BseMII CTCAG 3 cut(s) 329, 564, 918
BseRI GAGGAG 1 cut(s) 809
BseXI GCAGC 1 cut(s) 676
BseYI CCCAGC 1 cut(s) 986
BsgI GTGCAG 1 cut(s) 654
BshFI GGCC 1 cut(s) 673
BsiHKAI GWGCWC 1 cut(s) 304
BsiSI CCGG 2 cut(s) 1080, 1089
BslI CCNNNNNNNGG 2 cut(s) 52, 1082
BsnI GGCC 1 cut(s) 673
Bsp1286I GDGCHC 1 cut(s) 304
Bsp143I GATC 5 cut(s) 102, 121, 370, 405, 886
BspANI GGCC 1 cut(s) 673
BspCNI CTCAG 3 cut(s) 328, 565, 917
BspLI GGNNCC 4 cut(s) 104, 123, 1074, 1087
BspMI ACCTGC 1 cut(s) 919
BspPI GGATC 6 cut(s) 97, 110, 116, 129, 365, 881
BsrDI GCAATG 1 cut(s) 374
BssECI CCNNGG 2 cut(s) 890, 1089
BssMI GATC 5 cut(s) 102, 121, 370, 405, 886
Bst4CI ACNGT 1 cut(s) 247
Bst6I CTCTTC 1 cut(s) 5
BstBAI YACGTR 1 cut(s) 788
BstC8I GCNNGC 1 cut(s) 421
BstDEI CTNAG 4 cut(s) 315, 573, 904, 932
BstDSI CCRYGG 1 cut(s) 890
BstEII GGTNACC 1 cut(s) 779
BstENI CCTNNNNNAGG 1 cut(s) 1080
BstKTI GATC 5 cut(s) 105, 124, 373, 408, 889
BstMBI GATC 5 cut(s) 102, 121, 370, 405, 886
BstMWI GCNNNNNNNGC 2 cut(s) 445, 695
BstPI GGTNACC 1 cut(s) 779
BstSCI CCNGG 1 cut(s) 1088
BstSFI CTRYAG 1 cut(s) 471
BstV1I GCAGC 1 cut(s) 676
BstV2I GAAGAC 3 cut(s) 218, 950, 1064
BstX2I RGATCY 3 cut(s) 102, 121, 405
BstYI RGATCY 3 cut(s) 102, 121, 405
BsuRI GGCC 1 cut(s) 673
BtgI CCRYGG 1 cut(s) 890
BtsI GCAGTG 1 cut(s) 515
BtsIMutI CAGTG 1 cut(s) 515
BveI ACCTGC 1 cut(s) 919
Cac8I GCNNGC 1 cut(s) 421
CaiI CAGNNNCTG 1 cut(s) 911
Cfr13I GGNCC 1 cut(s) 1086
Csp6I GTAC 4 cut(s) 196, 312, 514, 901
CviAII CATG 3 cut(s) 76, 175, 811
CviQI GTAC 4 cut(s) 196, 312, 514, 901
DdeI CTNAG 4 cut(s) 315, 573, 904, 932
DpnI GATC 5 cut(s) 104, 123, 372, 407, 888
DpnII GATC 5 cut(s) 102, 121, 370, 405, 886
DraI TTTAAA 2 cut(s) 427, 970
Eam1104I CTCTTC 1 cut(s) 5
EarI CTCTTC 1 cut(s) 5
Eco147I AGGCCT 1 cut(s) 673
Eco47I GGWCC 1 cut(s) 1086
Eco57I CTGAAG 2 cut(s) 554, 744
Eco91I GGTNACC 1 cut(s) 779
EcoNI CCTNNNNNAGG 1 cut(s) 1080
EcoO65I GGTNACC 1 cut(s) 779
EcoT22I ATGCAT 1 cut(s) 620
FaeI CATG 3 cut(s) 79, 178, 814
FalI AAGNNNNNCTT 2 cut(s) 427, 459
FatI CATG 3 cut(s) 75, 174, 810
FblI GTMKAC 1 cut(s) 18
Fnu4HI GCNGC 1 cut(s) 690
Fsp4HI GCNGC 1 cut(s) 690
FspBI CTAG 3 cut(s) 297, 305, 1064
GluI GCNGC 1 cut(s) 690
GsaI CCCAGC 1 cut(s) 990
GsuI CTGGAG 1 cut(s) 720
HaeIII GGCC 1 cut(s) 673
HapII CCGG 2 cut(s) 1080, 1089
Hin1II CATG 3 cut(s) 79, 178, 814
HincII GTYRAC 1 cut(s) 832
HindII GTYRAC 1 cut(s) 832
HindIII AAGCTT 2 cut(s) 429, 1019
HinfI GANTC 3 cut(s) 131, 319, 1060
HpaII CCGG 2 cut(s) 1080, 1089
HphI GGTGA 2 cut(s) 506, 791
Hpy166II GTNNAC 5 cut(s) 19, 196, 552, 832, 977
Hpy188I TCNGA 9 cut(s) 117, 130, 136, 271, 318, 723, 763, 858, 907
Hpy188III TCNNGA 4 cut(s) 46, 305, 1025, 1064
Hpy8I GTNNAC 5 cut(s) 19, 196, 552, 832, 977
HpyAV CCTTC 5 cut(s) 82, 337, 621, 1045, 1057
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4IV ACGT 3 cut(s) 548, 787, 837
HpyCH4V TGCA 2 cut(s) 618, 635
HpyF10VI GCNNNNNNNGC 2 cut(s) 445, 695
HpyF3I CTNAG 4 cut(s) 315, 573, 904, 932
HpySE526I ACGT 3 cut(s) 548, 787, 837
Hsp92II CATG 3 cut(s) 79, 178, 814
Kzo9I GATC 5 cut(s) 102, 121, 370, 405, 886
LmnI GCTCC 2 cut(s) 176, 605
Lsp1109I GCAGC 1 cut(s) 676
LweI GCATC 4 cut(s) 159, 275, 406, 1021
MaeI CTAG 3 cut(s) 297, 305, 1064
MaeII ACGT 3 cut(s) 548, 787, 837
MaeIII GTNAC 4 cut(s) 27, 277, 779, 868
MalI GATC 5 cut(s) 104, 123, 372, 407, 888
MboI GATC 5 cut(s) 102, 121, 370, 405, 886
MfeI CAATTG 1 cut(s) 330
MflI RGATCY 3 cut(s) 102, 121, 405
MhlI GDGCHC 1 cut(s) 304
MluCI AATT 6 cut(s) 94, 137, 330, 850, 917, 1096
MlyI GAGTC 1 cut(s) 1054
MmeI TCCRAC 1 cut(s) 33
Mph1103I ATGCAT 1 cut(s) 620
MseI TTAA 7 cut(s) 65, 92, 426, 663, 840, 969, 1099
MslI CAYNNNNRTG 2 cut(s) 483, 962
MspI CCGG 2 cut(s) 1080, 1089
MspR9I CCNGG 1 cut(s) 1090
MunI CAATTG 1 cut(s) 330
MwoI GCNNNNNNNGC 2 cut(s) 445, 695
NciI CCSGG 1 cut(s) 1090
NdeII GATC 5 cut(s) 102, 121, 370, 405, 886
NlaIII CATG 3 cut(s) 79, 178, 814
NlaIV GGNNCC 4 cut(s) 104, 123, 1074, 1087
NmuCI GTSAC 2 cut(s) 27, 779
NsiI ATGCAT 1 cut(s) 620
PceI AGGCCT 1 cut(s) 673
PfeI GAWTC 2 cut(s) 131, 319
PflMI CCANNNNNTGG 1 cut(s) 52
PkrI GCNGC 1 cut(s) 691
PleI GAGTC 1 cut(s) 1054
PpsI GAGTC 1 cut(s) 1054
Ppu21I YACGTR 1 cut(s) 788
Psp1406I AACGTT 1 cut(s) 837
PspEI GGTNACC 1 cut(s) 779
PspFI CCCAGC 1 cut(s) 986
PspN4I GGNNCC 4 cut(s) 104, 123, 1074, 1087
PspPI GGNCC 1 cut(s) 1086
PstNI CAGNNNCTG 1 cut(s) 911
PsuI RGATCY 3 cut(s) 102, 121, 405
RsaI GTAC 4 cut(s) 197, 313, 515, 902
RsaNI GTAC 4 cut(s) 196, 312, 514, 901
RseI CAYNNNNRTG 2 cut(s) 483, 962
SaqAI TTAA 7 cut(s) 65, 92, 426, 663, 840, 969, 1099
SatI GCNGC 1 cut(s) 690
Sau3AI GATC 5 cut(s) 102, 121, 370, 405, 886
Sau96I GGNCC 1 cut(s) 1086
SchI GAGTC 1 cut(s) 1054
ScrFI CCNGG 1 cut(s) 1090
SduI GDGCHC 1 cut(s) 304
SfaNI GCATC 4 cut(s) 159, 275, 406, 1021
SfcI CTRYAG 1 cut(s) 471
SinI GGWCC 1 cut(s) 1086
SmiMI CAYNNNNRTG 2 cut(s) 483, 962
Sse9I AATT 6 cut(s) 94, 137, 330, 850, 917, 1096
SseBI AGGCCT 1 cut(s) 673
SspI AATATT 1 cut(s) 596
SspMI CTAG 3 cut(s) 297, 305, 1064
StuI AGGCCT 1 cut(s) 673
StyD4I CCNGG 1 cut(s) 1088
TaaI ACNGT 1 cut(s) 247
TaiI ACGT 3 cut(s) 551, 790, 840
TasI AATT 6 cut(s) 94, 137, 330, 850, 917, 1096
TatI WGTACW 1 cut(s) 900
TfiI GAWTC 2 cut(s) 131, 319
Tru1I TTAA 7 cut(s) 65, 92, 426, 663, 840, 969, 1099
Tru9I TTAA 7 cut(s) 65, 92, 426, 663, 840, 969, 1099
TscAI CASTG 1 cut(s) 515
TseFI GTSAC 2 cut(s) 27, 779
TseI GCWGC 1 cut(s) 689
Tsp45I GTSAC 2 cut(s) 27, 779
TspDTI ATGAA 7 cut(s) 64, 191, 224, 827, 1027, 1039, 1070
TspRI CASTG 1 cut(s) 515
Van91I CCANNNNNTGG 1 cut(s) 52
VpaK11BI GGWCC 1 cut(s) 1086
XagI CCTNNNNNAGG 1 cut(s) 1080
XapI RAATTY 3 cut(s) 94, 137, 917
XbaI TCTAGA 2 cut(s) 304, 1063
XmiI GTMKAC 1 cut(s) 18
XspI CTAG 3 cut(s) 297, 305, 1064
Zsp2I ATGCAT 1 cut(s) 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.