pycom16g00020

Prolyl endopeptidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0011215
Physical Location & Seq
Forward (+)
13249 .. 15223
1975 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g00020.2

Sequence Viewer

Length: 1050 bp
ATGGGAATGGGAATGAGAATGCATAAGCAGTGGCAGTGGCAGTGGCGCAGAGGAAACCTACTTCCGCTTGTCTACCTTCCAGTAGTCGTATTGTCGTCGTGTGGTGGTGGCGCCGGTTCCTCTCTAGGTTTCTCCGTCCGAGCGTCCACGTCATCAGAACCCACATCAAGAGTGAGGATGAGTGAGAGTAAGATGCAGCGGCCTCCGGTGGCGAAGAAGGTGGAGCATAAGATGGAGATGTTCGGCGACGTCAGAGTGGACAACTACTATTGGCTCCGCGACGACTCTCGCTCCGACCCCCAAGTCCTCTCTTACCTTCAACAAGAAAACGATTACACTCAATTCCTCATGTCTGGAAATAAGAAATTCGAAGATCAACTCTACTCTGAGATTAGGGGAAGGATCAAGGAGGATGATATATCTGCACCTGAACGAAAAGGCCCCTACTATTACTATCAAAGGACTCTCAAAGGTATGGAGTACGTTCAGCATTGTCGCCGCTTTATACCCAAAACCAAAAGCAGCGAGGGTGATGCGGACCCCCCAGTATCTGTGTATGATACCATGCCCACCGGACCTGATGCTCCTCCGGAGCATGTAATCTTGGATGAGAATGTTATGGCTAAAAATCATGCCTATTATAGCATTGCTGCTTTTAAGGTTAGCCCAAACAACAATTTGGTGGCATATGCAGAAGACACCAAAGGAAATGAAATCTATACAGTTTATGTCATTGATGCCGAGACAGGTGCTCCTGTGGGTCTGCCTCTTGTTGGTACAACATCCTATCTTGAATGGGCCGGCAACGAGGCATTAGTTTACATTACAATGGACGAGACCCTCCGCCCTGACAAGGCATGGTTACATAAGTTGGGGACAGAACAGTCAAGTGACTCCTGCCTCTATCATGAACAGGATGATATGTTTTCAATTGATCTTCACGCTTCTGAGAGCAAAAAGTTCTTGTTTGTTGCATCCGAAAGTAAAACTACAAGATTCAACTTTTACATGATGATCATTCAAAGCCTGAAGATGGGCTTATGGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05142 ko05143 map05142 map05143
Pfam Domains
Protein Families
Peptidase_S9 Peptidase_S9_N

Protein Analysis

350

Amino Acids

39.95

Weight (kDa)

5.82

Isoelectric Point (pI)

56.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011215)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50380 AT1G50380
fragaria_vesca FvH4_4g37301
malus_domestica MD16G1004600.v1.1
prunus_persica Prupe.1G351000_v2.0.a1 Prupe.1G351200_v2.0.a1 Prupe.1G351200_v2.0.a1
pyrus_communis pycom16g00020 pycom16g00030
rosa_chinensis RchiOBHm_Chr4g0447131 RchiOBHm_Chr5g0037361
rosa_laevigata RLG00000005602
rosa_multiflora Rmu_ssc0000178.1_g000010
rosa_roxburghii Rroxscaffold_5G00387470
rosa_rugosa Rorug04G0378500
rosa_samantha Rh4AG439600 Rh4BG448700 Rh4CG465700 Rh4DG447100
rosa_wichuraiana Rw4G037610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 302, 883
AatII GACGTC 1 cut(s) 252
AccB1I GGYRCC 1 cut(s) 110
AccI GTMKAC 1 cut(s) 72
AccII CGCG 1 cut(s) 279
AccIII TCCGGA 1 cut(s) 589
AciI CCGC 6 cut(s) 65, 199, 277, 499, 536, 844
AclWI GGATC 1 cut(s) 410
AcsI RAATTY 1 cut(s) 365
AcuI CTGAAG 1 cut(s) 1049
AcyI GRCGYC 2 cut(s) 111, 249
AfaI GTAC 2 cut(s) 482, 778
AfiI CCNNNNNNNGG 3 cut(s) 773, 853, 1033
AgsI TTSAA 5 cut(s) 320, 794, 930, 1000, 1022
AjiI CACGTC 1 cut(s) 150
Alw21I GWGCWC 1 cut(s) 754
Alw26I GTCTC 2 cut(s) 737, 830
AlwI GGATC 1 cut(s) 410
AlwNI CAGNNNCTG 1 cut(s) 551
Aor13HI TCCGGA 1 cut(s) 589
AoxI GGCC 3 cut(s) 200, 439, 798
ApeKI GCWGC 3 cut(s) 196, 522, 650
ApoI RAATTY 1 cut(s) 365
AspLEI GCGC 2 cut(s) 48, 113
AspS9I GGNCC 4 cut(s) 440, 538, 575, 798
AsuHPI GGTGA 1 cut(s) 542
AsuII TTCGAA 1 cut(s) 369
AvaII GGWCC 2 cut(s) 538, 575
BanI GGYRCC 1 cut(s) 110
BbsI GAAGAC 1 cut(s) 702
Bbv12I GWGCWC 1 cut(s) 754
BbvI GCAGC 3 cut(s) 208, 534, 637
BccI CCATC 2 cut(s) 226, 1027
BcgI CGANNNNNNTGC 4 cut(s) 515, 549, 731, 765
BclI TGATCA 1 cut(s) 1014
BcoDI GTCTC 2 cut(s) 737, 830
BfaI CTAG 1 cut(s) 125
BfoI RGCGCY 1 cut(s) 114
BisI GCNGC 5 cut(s) 197, 200, 499, 523, 651
BlsI GCNGC 5 cut(s) 198, 201, 500, 524, 652
Bme18I GGWCC 2 cut(s) 538, 575
BmgBI CACGTC 1 cut(s) 150
BmgT120I GGNCC 4 cut(s) 440, 538, 575, 798
BmiI GGNNCC 5 cut(s) 112, 118, 275, 442, 540
BmrI ACTGGG 1 cut(s) 539
BmsI GCATC 5 cut(s) 183, 523, 571, 727, 983
BmuI ACTGGG 1 cut(s) 539
BpiI GAAGAC 1 cut(s) 702
Bpu14I TTCGAA 1 cut(s) 369
BsaHI GRCGYC 2 cut(s) 111, 249
BsaI GGTCTC 1 cut(s) 830
BsaWI WCCGGW 3 cut(s) 205, 572, 589
BsaXI ACNNNNNCTCC 4 cut(s) 275, 305, 736, 766
Bsc4I CCNNNNNNNGG 3 cut(s) 773, 853, 1033
Bse118I RCCGGY 2 cut(s) 113, 800
Bse1I ACTGG 2 cut(s) 80, 545
Bse3DI GCAATG 1 cut(s) 645
BseAI TCCGGA 1 cut(s) 589
BseGI GGATG 6 cut(s) 183, 418, 613, 782, 922, 974
BseLI CCNNNNNNNGG 3 cut(s) 773, 853, 1033
BseMI GCAATG 1 cut(s) 645
BseMII CTCAG 2 cut(s) 378, 939
BseNI ACTGG 2 cut(s) 80, 545
BseRI GAGGAG 1 cut(s) 576
BseXI GCAGC 3 cut(s) 208, 534, 637
BsgI GTGCAG 1 cut(s) 408
Bsh1236I CGCG 1 cut(s) 279
BshFI GGCC 3 cut(s) 202, 441, 800
BshNI GGYRCC 1 cut(s) 110
BsiHKAI GWGCWC 1 cut(s) 754
BsiSI CCGG 5 cut(s) 114, 206, 573, 590, 801
BslFI GGGAC 1 cut(s) 889
BslI CCNNNNNNNGG 3 cut(s) 773, 853, 1033
BsmAI GTCTC 2 cut(s) 737, 830
BsmFI GGGAC 1 cut(s) 889
BsmI GAATGC 1 cut(s) 24
BsnI GGCC 3 cut(s) 202, 441, 800
Bso31I GGTCTC 1 cut(s) 830
Bsp119I TTCGAA 1 cut(s) 369
Bsp1286I GDGCHC 1 cut(s) 754
Bsp13I TCCGGA 1 cut(s) 589
Bsp143I GATC 4 cut(s) 373, 402, 934, 1014
BspACI CCGC 6 cut(s) 65, 199, 277, 499, 536, 844
BspANI GGCC 3 cut(s) 202, 441, 800
BspCNI CTCAG 2 cut(s) 379, 940
BspEI TCCGGA 1 cut(s) 589
BspFNI CGCG 1 cut(s) 279
BspHI TCATGA 1 cut(s) 907
BspLI GGNNCC 5 cut(s) 112, 118, 275, 442, 540
BspPI GGATC 1 cut(s) 410
BspT104I TTCGAA 1 cut(s) 369
BspT107I GGYRCC 1 cut(s) 110
BspTNI GGTCTC 1 cut(s) 830
BsrDI GCAATG 1 cut(s) 645
BsrFI RCCGGY 2 cut(s) 113, 800
BsrI ACTGG 2 cut(s) 80, 545
BssAI RCCGGY 2 cut(s) 113, 800
BssMI GATC 4 cut(s) 373, 402, 934, 1014
BssNI GRCGYC 2 cut(s) 111, 249
Bst4CI ACNGT 2 cut(s) 724, 885
BstACI GRCGYC 2 cut(s) 111, 249
BstBI TTCGAA 1 cut(s) 369
BstC8I GCNNGC 1 cut(s) 802
BstDEI CTNAG 2 cut(s) 387, 948
BstF5I GGATG 6 cut(s) 183, 418, 613, 782, 922, 974
BstFNI CGCG 1 cut(s) 279
BstH2I RGCGCY 1 cut(s) 114
BstHHI GCGC 2 cut(s) 48, 113
BstKTI GATC 4 cut(s) 376, 405, 937, 1017
BstMAI GTCTC 2 cut(s) 737, 830
BstMBI GATC 4 cut(s) 373, 402, 934, 1014
BstNSI RCATGY 1 cut(s) 599
BstUI CGCG 1 cut(s) 279
BstV1I GCAGC 3 cut(s) 208, 534, 637
BstV2I GAAGAC 1 cut(s) 702
BsuRI GGCC 3 cut(s) 202, 441, 800
BtrI CACGTC 1 cut(s) 150
BtsCI GGATG 6 cut(s) 183, 418, 613, 782, 922, 974
BtsI GCAGTG 3 cut(s) 35, 41, 47
BtsIMutI CAGTG 3 cut(s) 35, 41, 47
Cac8I GCNNGC 1 cut(s) 802
CaiI CAGNNNCTG 1 cut(s) 551
CciI TCATGA 1 cut(s) 907
CfoI GCGC 2 cut(s) 48, 113
Cfr10I RCCGGY 2 cut(s) 113, 800
Cfr13I GGNCC 4 cut(s) 440, 538, 575, 798
CseI GACGC 1 cut(s) 132
Csp6I GTAC 2 cut(s) 481, 777
CviAII CATG 7 cut(s) 349, 565, 596, 632, 858, 908, 1009
CviJI RGCY 8 cut(s) 202, 274, 441, 623, 666, 800, 1026, 1038
CviKI_1 RGCY 8 cut(s) 202, 274, 441, 623, 666, 800, 1026, 1038
CviQI GTAC 2 cut(s) 481, 777
DdeI CTNAG 2 cut(s) 387, 948
DinI GGCGCC 1 cut(s) 112
DpnI GATC 4 cut(s) 375, 404, 936, 1016
DpnII GATC 4 cut(s) 373, 402, 934, 1014
DrdI GACNNNNNNGTC 2 cut(s) 302, 883
DseDI GACNNNNNNGTC 2 cut(s) 302, 883
EciI GGCGGA 1 cut(s) 833
Eco31I GGTCTC 1 cut(s) 830
Eco47I GGWCC 2 cut(s) 538, 575
Eco57I CTGAAG 1 cut(s) 1049
EcoO109I RGGNCCY 1 cut(s) 440
EcoT22I ATGCAT 1 cut(s) 24
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
FaeI CATG 7 cut(s) 352, 568, 599, 635, 861, 911, 1012
FalI AAGNNNNNCTT 1 cut(s) 1022
FaqI GGGAC 1 cut(s) 889
FatI CATG 7 cut(s) 348, 564, 595, 631, 857, 907, 1008
FauNDI CATATG 1 cut(s) 688
FbaI TGATCA 1 cut(s) 1014
FblI GTMKAC 1 cut(s) 72
Fnu4HI GCNGC 5 cut(s) 197, 200, 499, 523, 651
FokI GGATG 6 cut(s) 190, 425, 620, 769, 929, 961
Fsp4HI GCNGC 5 cut(s) 197, 200, 499, 523, 651
FspBI CTAG 1 cut(s) 125
GlaI GCGC 2 cut(s) 47, 112
GluI GCNGC 5 cut(s) 197, 200, 499, 523, 651
HaeII RGCGCY 1 cut(s) 114
HaeIII GGCC 3 cut(s) 202, 441, 800
HapII CCGG 5 cut(s) 114, 206, 573, 590, 801
HgaI GACGC 1 cut(s) 132
HhaI GCGC 2 cut(s) 48, 113
Hin1I GRCGYC 2 cut(s) 111, 249
Hin1II CATG 7 cut(s) 352, 568, 599, 635, 861, 911, 1012
Hin6I GCGC 2 cut(s) 46, 111
HinP1I GCGC 2 cut(s) 46, 111
HinfI GANTC 4 cut(s) 284, 463, 893, 996
HpaII CCGG 5 cut(s) 114, 206, 573, 590, 801
HphI GGTGA 1 cut(s) 542
Hpy166II GTNNAC 4 cut(s) 73, 147, 259, 820
Hpy188I TCNGA 8 cut(s) 140, 157, 254, 295, 388, 949, 979, 1049
Hpy188III TCNNGA 5 cut(s) 168, 354, 590, 791, 908
Hpy8I GTNNAC 4 cut(s) 73, 147, 259, 820
Hpy99I CGWCG 3 cut(s) 100, 251, 284
HpyAV CCTTC 4 cut(s) 86, 211, 326, 393
HpyCH4III ACNGT 2 cut(s) 724, 885
HpyCH4IV ACGT 3 cut(s) 149, 249, 483
HpyCH4V TGCA 5 cut(s) 22, 196, 425, 692, 974
HpyF3I CTNAG 2 cut(s) 387, 948
HpySE526I ACGT 3 cut(s) 149, 249, 483
Hsp92I GRCGYC 2 cut(s) 111, 249
Hsp92II CATG 7 cut(s) 352, 568, 599, 635, 861, 911, 1012
HspAI GCGC 2 cut(s) 46, 111
KasI GGCGCC 1 cut(s) 110
Kpn2I TCCGGA 1 cut(s) 589
KroI GCCGGC 1 cut(s) 800
KroNI GCCGGC 1 cut(s) 802
Ksp22I TGATCA 1 cut(s) 1014
Kzo9I GATC 4 cut(s) 373, 402, 934, 1014
LmnI GCTCC 6 cut(s) 223, 279, 296, 589, 592, 757
Lsp1109I GCAGC 3 cut(s) 208, 534, 637
LweI GCATC 5 cut(s) 183, 523, 571, 727, 983
MaeI CTAG 1 cut(s) 125
MaeII ACGT 3 cut(s) 149, 249, 483
MaeIII GTNAC 2 cut(s) 861, 890
MalI GATC 4 cut(s) 375, 404, 936, 1016
MboI GATC 4 cut(s) 373, 402, 934, 1014
MboII GAAGA 5 cut(s) 226, 383, 707, 929, 1042
MfeI CAATTG 1 cut(s) 930
MhlI GDGCHC 1 cut(s) 754
MluCI AATT 4 cut(s) 341, 365, 676, 930
Mly113I GGCGCC 1 cut(s) 111
MlyI GAGTC 3 cut(s) 278, 457, 887
MmeI TCCRAC 1 cut(s) 318
Mph1103I ATGCAT 1 cut(s) 24
MroI TCCGGA 1 cut(s) 589
MroNI GCCGGC 1 cut(s) 800
MseI TTAA 1 cut(s) 657
MslI CAYNNNNRTG 1 cut(s) 827
MspA1I CMGCKG 1 cut(s) 199
MspI CCGG 5 cut(s) 114, 206, 573, 590, 801
MunI CAATTG 1 cut(s) 930
Mva1269I GAATGC 1 cut(s) 24
MvnI CGCG 1 cut(s) 279
NaeI GCCGGC 1 cut(s) 802
NarI GGCGCC 1 cut(s) 111
NdeI CATATG 1 cut(s) 688
NdeII GATC 4 cut(s) 373, 402, 934, 1014
NgoMIV GCCGGC 1 cut(s) 800
NlaIII CATG 7 cut(s) 352, 568, 599, 635, 861, 911, 1012
NlaIV GGNNCC 5 cut(s) 112, 118, 275, 442, 540
NmeAIII GCCGAG 1 cut(s) 766
NmuCI GTSAC 1 cut(s) 890
NsiI ATGCAT 1 cut(s) 24
NspI RCATGY 1 cut(s) 599
NspV TTCGAA 1 cut(s) 369
PagI TCATGA 1 cut(s) 907
PctI GAATGC 1 cut(s) 24
PdiI GCCGGC 1 cut(s) 802
PfeI GAWTC 1 cut(s) 996
PkrI GCNGC 5 cut(s) 198, 201, 500, 524, 652
PleI GAGTC 3 cut(s) 278, 457, 887
PluTI GGCGCC 1 cut(s) 114
PpsI GAGTC 3 cut(s) 278, 457, 887
PspN4I GGNNCC 5 cut(s) 112, 118, 275, 442, 540
PspPI GGNCC 4 cut(s) 440, 538, 575, 798
PstNI CAGNNNCTG 1 cut(s) 551
RsaI GTAC 2 cut(s) 482, 778
RsaNI GTAC 2 cut(s) 481, 777
RseI CAYNNNNRTG 1 cut(s) 827
SaqAI TTAA 1 cut(s) 657
SatI GCNGC 5 cut(s) 197, 200, 499, 523, 651
Sau3AI GATC 4 cut(s) 373, 402, 934, 1014
Sau96I GGNCC 4 cut(s) 440, 538, 575, 798
SchI GAGTC 3 cut(s) 278, 457, 887
SduI GDGCHC 1 cut(s) 754
SfaNI GCATC 5 cut(s) 183, 523, 571, 727, 983
SfoI GGCGCC 1 cut(s) 112
SfuI TTCGAA 1 cut(s) 369
SinI GGWCC 2 cut(s) 538, 575
SmiMI CAYNNNNRTG 1 cut(s) 827
Sse9I AATT 4 cut(s) 341, 365, 676, 930
SsiI CCGC 6 cut(s) 65, 199, 277, 499, 536, 844
SspDI GGCGCC 1 cut(s) 110
SspMI CTAG 1 cut(s) 125
TaaI ACNGT 2 cut(s) 724, 885
TaiI ACGT 3 cut(s) 152, 252, 486
TaqI TCGA 1 cut(s) 369
TasI AATT 4 cut(s) 341, 365, 676, 930
TauI GCSGC 2 cut(s) 202, 501
TfiI GAWTC 1 cut(s) 996
Tru1I TTAA 1 cut(s) 657
Tru9I TTAA 1 cut(s) 657
TscAI CASTG 3 cut(s) 35, 41, 47
TseFI GTSAC 1 cut(s) 890
TseI GCWGC 3 cut(s) 196, 522, 650
Tsp45I GTSAC 1 cut(s) 890
TspDTI ATGAA 2 cut(s) 726, 924
TspGWI ACGGA 1 cut(s) 124
TspRI CASTG 3 cut(s) 35, 41, 47
VpaK11BI GGWCC 2 cut(s) 538, 575
XapI RAATTY 1 cut(s) 365
XceI RCATGY 1 cut(s) 599
XmiI GTMKAC 1 cut(s) 72
XspI CTAG 1 cut(s) 125
ZraI GACGTC 1 cut(s) 250
Zsp2I ATGCAT 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.