Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0015611

Physical Location & Seq

Genomic Coordinates Forward (+)

1210771 .. 1213170

Gene Structure 2400 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1015600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2400 bp

ATGTCCACCTTCCTCCTCTCCATCCTCCTCGCCTTCATCCTCTCAAACCTGCCACCCTCCTCCGCCCAACAACTCAACTCCTTCTCCTCCAAAGACTCCCCATGGACTCCATCCCAGCAGAACAAGACCCTCCTCTCCCCAAACTCCCTCTTTGCCGCCGGTTTCCTAAGCCTACCCAATTCCCCCAACCTCTTCAACTTCTCAGTTTGGTACCACAACATCTCCGCCCCCAACTCCGTCGTCTGGACCGCCAACCCAAAATCCCCATTAGCCCCCTCCGCAACCCTCGTCATCACGGCAGCCGGCGTCCTCCGTCTCACTAACTCTTCCGCCGCCGCTGGCGGTGGCAACTTGTGGCCAGGACCTTCTTCTTCGAACCCAAACACCACCAGACTCCTCCTCCGCAACGACGGAAACTTAATTTACGGCAAATGGGAGAGCTTCGCCTTCCCCACTGACACCGTGTTGCCGAACCAGAGCATGACCGGAGCTAATTTCACCCTTCTTTCCAAAAACGGCAAGTTCAGCGTCGTCAACGCGAGTTGTTTAGTCTTCAACAACACTGACGTCTACCAGAATCTCACCAACGCTTTCCAGTCTCTGGGCTCCGAAGGCAAAGTGCAGCAGGCAAACGGCGCCTCGTTTATTGCCTCCGATTTCGGCCTGAATCGATCTCGAAGACTGACGATCGACGACGACGGGAATTTCAGAATTTACAGCTTCGACCCCAGTCTGCGGCAATGGAACATCGTCTGGCAAGCCGGGTACGAATTGTGCCAAGTCCACGGCACGTGTGGGCCGAACGCCATCTGCGTCAGCGACGGTTCGAGCTCCTCTTACTGCGTGTGCCCGCCGGGATTTAGAGAATCCGCCGGCGGAATCAAAGACGGAGGGTGCGAAAGGAAAATTAAGCTCACGAATTTGGGGAACACCAGATTTGAGCGGCTTGATTACGTGAATTTCACGGGCGGGTCGAACCAGACCAATTGGCCCGCCACCAATTTCAGCGTCTGCGAATCAAGATGCCTGGTGAGAAACAATTGCCTCGGATTCATGTTCAAATACGACGGCAAAGGCTACTGCGTGCTCCAGCTCGAGAGATTGTTATACGGGTACTGGTCGCCCGGATCCGAAACCGCTATGTTTCTCCGGGTCGACAAATCGGAGACTGACAGGTCGAATTTTACAGGGATGACGGAGCTGCTGGAGACGACGTGTCCCGTTCAGATAAGACTCCCGCTTCCGCCGGAGGATTCGAACGCGACGACAAGGAACATTGTGATTATATGCACCCTTTTTGCGGCGGAGCTGATTTCCGGCGTGCTTTTCTTCTGGGCATTTATAAAGAAGTACATCAAGTACAGAGACATGGCTCGGACTCTGGGTCTCGAGTTCCTCCCCGCCGGGGGCCCAAAGAGGTTCTCGTACGCTGAACTTAAAGCCGCCACCAAAGACTTCTCGAACCTCATCGGCAAAGGCGGGTTTGGAGACGTTTACAAAGGCGTGTTAACCGATCAGCGCGTGGTGGCAGTTAAGTGTTTGAAGCATGTCGCCGGCGGTGACGCCGAGTTCTGGGCGGAGGTAACGATTATTGCCCGGATGCACCACCTCAACTTGGTCAGACTTTGGGGCTTTTGCGCGGAAAAGGGTCAACGGATCTTAGTCTATGAGTACGTCCCAAATGGATCCCTCGACAAGTACCTCTTCCAACCGGGTCGGGTTACGCCGTCAGACTCGGTGGAGGAAACGGGTGTTCTGATCGATGACGAGCGGAAACCGATACTCGATTGGGGGATCCGATACCGAATTGCTTTGGGTGTGGCGAGATCGATCGCGTACCTCCACGAGGAGTGCTTGGAGTGGGTTTTGCACTGCGACATCAAGCCGGAGAACATTCTCTTGGGCGACGATTTCTGCCCCAAGATATCGGATTTCGGGCTGGCGAAGCTGAAGAAAAAGGAGGACATGGTGACGATATCTCGGATGCACGGGACCCGCGGGTACATGGCGCCGGAGTGGATCAAGGCGGACCAGATCACGCCCAAGGCTGACGTGTACAGCTTCGGGATGGTGCTGTTGGAACTGGTGAGTGGGGTCCGCAACACCGAGATCCAAGGGTCGATGATCGAGAGCGACGACTGGTACTTTCCGAGATGGGCGTTCGATAAGGTGTTTAAGGAGATGAACGTGGAGGATATTCTGGACCGTCGGATCAAGAACTCGTACGATAGCAGGGTGCATTTCGATGCGGTGAATCGGATGGTGCAGACGGCGATGTGGTGCTTGCAGGATCGGCCGGAGATGCGGCCGTCGATGGGGAAGGTGGCGAAGATGTTGGAGGGGACGGTGGATATCACGGAGCCCAAAAAGCCCACTATATTTTTCCTCAGCGACGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin PAN_2 Pkinase S_locus_glycop

Protein Analysis

800

Amino Acids

89.1

Weight (kDa)

7.42

Isoelectric Point (pI)

37.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	80 - 162	5.4e-08	D-mannose binding lectin
S_locus_glycop	PF00954	221 - 289	3.4e-10	S-locus glycoprotein domain
PK_Tyr_Ser-Thr	PF07714	487 - 778	5.9e-43	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	488 - 775	4.3e-44	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015611)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g33230
malus_domestica	MD16G1015600.v1.1
prunus_persica	Prupe.1G336000_v2.0.a1
pyrus_communis	pycom15g28260 pycom16g01450
rosa_chinensis	RchiOBHm_Chr4g0442381
rosa_laevigata	RLG00000006014
rosa_multiflora	Rmu_co8314767.1_g000001 Rmu_co8434117.1_g000001 Rmu_sc0000124.1_g000043
rosa_roxburghii	Rroxscaffold_5G00383230
rosa_rugosa	Rorug04G0340700
rosa_samantha	Rh4AG392300 Rh4BG406000 Rh4CG421100 Rh4DG399100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1343
AasI	GACNNNNNNGTC	1 cut(s)	1174
AatII	GACGTC	1 cut(s)	570
Acc36I	ACCTGC	1 cut(s)	57
Acc65I	GGTACC	1 cut(s)	210
AccB1I	GGYRCC	3 cut(s)	210, 635, 2008
AccB7I	CCANNNNNTGG	1 cut(s)	601
AccBSI	CCGCTC	2 cut(s)	943, 1771
AccI	GTMKAC	2 cut(s)	570, 1155
AccII	CGCG	6 cut(s)	539, 1262, 1521, 1640, 1835, 1998
AcoI	YGGCCR	3 cut(s)	356, 2294, 2306
AcsI	RAATTY	5 cut(s)	703, 711, 919, 958, 1180
AcuI	CTGAAG	1 cut(s)	1970
AcvI	CACGTG	1 cut(s)	792
AcyI	GRCGYC	5 cut(s)	306, 567, 636, 1563, 2009
AflIII	ACRYGT	3 cut(s)	791, 1214, 2052
AgsI	TTSAA	4 cut(s)	196, 556, 1060, 1543
AhdI	GACNNNNNGTC	2 cut(s)	1215, 1383
AjiI	CACGTC	2 cut(s)	1215, 2053
AjnI	CCWGG	2 cut(s)	358, 1026
AjuI	GAANNNNNNNTTGG	2 cut(s)	1883, 1915
Alw21I	GWGCWC	2 cut(s)	833, 1089
Alw26I	GTCTC	7 cut(s)	320, 603, 1160, 1202, 1359, 1391, 1482
AlwNI	CAGNNNCTG	2 cut(s)	601, 1011
Ama87I	CYCGRG	2 cut(s)	1094, 1388
AoxI	GGCC	7 cut(s)	356, 661, 797, 989, 1408, 2294, 2306
ApaI	GGGCCC	1 cut(s)	1412
ApeKI	GCWGC	3 cut(s)	299, 622, 1201
ApoI	RAATTY	5 cut(s)	703, 711, 919, 958, 1180
Asp718I	GGTACC	1 cut(s)	210
AspLEI	GCGC	4 cut(s)	638, 1521, 1640, 2011
AsuC2I	CCSGG	7 cut(s)	763, 855, 1125, 1151, 1405, 1597, 1713
AsuHPI	GGTGA	7 cut(s)	490, 574, 1042, 1571, 1981, 2098, 2263
AsuII	TTCGAA	2 cut(s)	374, 1256
AvaI	CYCGRG	2 cut(s)	1094, 1388
AvaII	GGWCC	6 cut(s)	246, 362, 1992, 2029, 2095, 2203
BaeGI	GKGCMC	2 cut(s)	851, 1412
BalI	TGGCCA	1 cut(s)	358
BamHI	GGATCC	3 cut(s)	1127, 1685, 1794
BanI	GGYRCC	3 cut(s)	210, 635, 2008
BanII	GRGCYC	4 cut(s)	608, 833, 1412, 2364
BauI	CACGAG	1 cut(s)	1844
BbrPI	CACGTG	1 cut(s)	792
BbsI	GAAGAC	2 cut(s)	544, 685
Bbv12I	GWGCWC	2 cut(s)	833, 1089
BbvCI	CCTCAGC	1 cut(s)	2387
BbvI	GCAGC	3 cut(s)	311, 634, 1188
BccI	CCATC	7 cut(s)	29, 118, 815, 2062, 2148, 2254, 2308
BceAI	ACGGC	9 cut(s)	312, 442, 532, 649, 802, 1084, 1711, 2286, 2293
BciT130I	CCWGG	2 cut(s)	360, 1028
BcnI	CCSGG	7 cut(s)	763, 855, 1125, 1151, 1405, 1597, 1713
BcoDI	GTCTC	7 cut(s)	320, 603, 1160, 1202, 1359, 1391, 1482
BfaI	CTAG	1 cut(s)	2398
BfoI	RGCGCY	2 cut(s)	639, 2012
BfuAI	ACCTGC	1 cut(s)	57
Bme1390I	CCNGG	9 cut(s)	360, 763, 855, 1028, 1125, 1151, 1405, 1597, 1713
Bme18I	GGWCC	6 cut(s)	246, 362, 1992, 2029, 2095, 2203
BmeRI	GACNNNNNGTC	2 cut(s)	1215, 1383
BmeT110I	CYCGRG	2 cut(s)	1094, 1388
BmgBI	CACGTC	2 cut(s)	1215, 2053
BmrFI	CCNGG	9 cut(s)	360, 763, 855, 1028, 1125, 1151, 1405, 1597, 1713
BmrI	ACTGGG	1 cut(s)	723
BmsI	GCATC	5 cut(s)	1013, 1590, 1974, 2236, 2292
BmuI	ACTGGG	1 cut(s)	723
BoxI	GACNNNNGTC	1 cut(s)	729
BpiI	GAAGAC	2 cut(s)	544, 685
BpmI	CTGGAG	2 cut(s)	1073, 1226
Bpu10I	CCTNAGC	2 cut(s)	167, 2387
Bpu14I	TTCGAA	2 cut(s)	374, 1256
BpuMI	CCSGG	7 cut(s)	763, 855, 1125, 1151, 1405, 1597, 1713
Bsa29I	ATCGAT	3 cut(s)	670, 1761, 1829
BsaAI	YACGTR	2 cut(s)	792, 955
BsaHI	GRCGYC	5 cut(s)	306, 567, 636, 1563, 2009
BsaI	GGTCTC	1 cut(s)	1391
BsaJI	CCNNGG	7 cut(s)	101, 784, 1045, 1404, 1996, 2043, 2113
BsaWI	WCCGGW	1 cut(s)	485
BsaXI	ACNNNNNCTCC	8 cut(s)	68, 98, 206, 236, 384, 414, 1199, 1229
Bse118I	RCCGGY	4 cut(s)	158, 302, 872, 1553
Bse1I	ACTGG	5 cut(s)	595, 729, 1121, 2088, 2144
Bse3DI	GCAATG	1 cut(s)	746
BseBI	CCWGG	2 cut(s)	360, 1028
BseCI	ATCGAT	3 cut(s)	670, 1761, 1829
BseDI	CCNNGG	7 cut(s)	101, 784, 1045, 1404, 1996, 2043, 2113
BseGI	GGATG	8 cut(s)	21, 36, 110, 1197, 1605, 1989, 2073, 2265
BseMI	GCAATG	1 cut(s)	746
BseMII	CTCAG	1 cut(s)	216
BseNI	ACTGG	5 cut(s)	595, 729, 1121, 2088, 2144
BseRI	GAGGAG	9 cut(s)	5, 17, 49, 76, 122, 386, 389, 823, 1862
BseSI	GKGCMC	2 cut(s)	851, 1412
BseX3I	CGGCCG	2 cut(s)	2294, 2306
BseXI	GCAGC	3 cut(s)	311, 634, 1188
BseYI	CCCAGC	1 cut(s)	114
BsgI	GTGCAG	2 cut(s)	641, 2285
Bsh1236I	CGCG	6 cut(s)	539, 1262, 1521, 1640, 1835, 1998
Bsh1285I	CGRYCG	4 cut(s)	690, 1833, 2297, 2309
BshFI	GGCC	7 cut(s)	358, 663, 799, 991, 1410, 2296, 2308
BshNI	GGYRCC	3 cut(s)	210, 635, 2008
BshVI	ATCGAT	3 cut(s)	670, 1761, 1829
BsiEI	CGRYCG	4 cut(s)	690, 1833, 2297, 2309
BsiHKAI	GWGCWC	2 cut(s)	833, 1089
BsiHKCI	CYCGRG	2 cut(s)	1094, 1388
BsiWI	CGTACG	2 cut(s)	1425, 2223
BslFI	GGGAC	4 cut(s)	1203, 1661, 2005, 2356
BsmAI	GTCTC	7 cut(s)	320, 603, 1160, 1202, 1359, 1391, 1482
BsmBI	CGTCTC	3 cut(s)	320, 1202, 1482
BsmFI	GGGAC	4 cut(s)	1203, 1661, 2005, 2356
BsnI	GGCC	7 cut(s)	358, 663, 799, 991, 1410, 2296, 2308
Bso31I	GGTCTC	1 cut(s)	1391
BsoBI	CYCGRG	2 cut(s)	1094, 1388
Bsp119I	TTCGAA	2 cut(s)	374, 1256
Bsp120I	GGGCCC	1 cut(s)	1408
Bsp1286I	GDGCHC	6 cut(s)	608, 833, 851, 1089, 1412, 2364
Bsp1407I	TGTACA	1 cut(s)	2055
Bsp19I	CCATGG	1 cut(s)	101
BspANI	GGCC	7 cut(s)	358, 663, 799, 991, 1410, 2296, 2308
BspCNI	CTCAG	2 cut(s)	215, 2400
BspDI	ATCGAT	3 cut(s)	670, 1761, 1829
BspFNI	CGCG	6 cut(s)	539, 1262, 1521, 1640, 1835, 1998
BspMI	ACCTGC	1 cut(s)	57
BspT104I	TTCGAA	2 cut(s)	374, 1256
BspT107I	GGYRCC	3 cut(s)	210, 635, 2008
BspTNI	GGTCTC	1 cut(s)	1391
BsrBI	CCGCTC	2 cut(s)	943, 1771
BsrDI	GCAATG	1 cut(s)	746
BsrFI	RCCGGY	4 cut(s)	158, 302, 872, 1553
BsrGI	TGTACA	1 cut(s)	2055
BsrI	ACTGG	5 cut(s)	595, 729, 1121, 2088, 2144
BssAI	RCCGGY	4 cut(s)	158, 302, 872, 1553
BssECI	CCNNGG	7 cut(s)	101, 784, 1045, 1404, 1996, 2043, 2113
BssNI	GRCGYC	5 cut(s)	306, 567, 636, 1563, 2009
BssSI	CACGAG	1 cut(s)	1844
BssT1I	CCWWGG	3 cut(s)	101, 2043, 2113
Bst2BI	CACGAG	1 cut(s)	1844
Bst2UI	CCWGG	2 cut(s)	360, 1028
Bst4CI	ACNGT	4 cut(s)	463, 824, 2207, 2347
Bst6I	CTCTTC	3 cut(s)	197, 331, 1709
BstACI	GRCGYC	5 cut(s)	306, 567, 636, 1563, 2009
BstAUI	TGTACA	1 cut(s)	2055
BstBAI	YACGTR	2 cut(s)	792, 955
BstBI	TTCGAA	2 cut(s)	374, 1256
BstDEI	CTNAG	4 cut(s)	167, 202, 1660, 2387
BstDSI	CCRYGG	3 cut(s)	101, 784, 1996
BstENI	CCTNNNNNAGG	1 cut(s)	1844
BstF5I	GGATG	8 cut(s)	21, 36, 110, 1197, 1605, 1989, 2073, 2265
BstFNI	CGCG	6 cut(s)	539, 1262, 1521, 1640, 1835, 1998
BstH2I	RGCGCY	2 cut(s)	639, 2012
BstHHI	GCGC	4 cut(s)	638, 1521, 1640, 2011
BstMAI	GTCTC	7 cut(s)	320, 603, 1160, 1202, 1359, 1391, 1482
BstMCI	CGRYCG	4 cut(s)	690, 1833, 2297, 2309
BstMWI	GCNNNNNNNGC	8 cut(s)	278, 525, 612, 635, 1945, 2293, 2302, 2368
BstNI	CCWGG	2 cut(s)	360, 1028
BstNSI	RCATGY	1 cut(s)	1550
BstPAI	GACNNNNGTC	1 cut(s)	729
BstSCI	CCNGG	9 cut(s)	358, 761, 853, 1026, 1123, 1149, 1403, 1595, 1711
BstSLI	GKGCMC	2 cut(s)	851, 1412
BstUI	CGCG	6 cut(s)	539, 1262, 1521, 1640, 1835, 1998
BstV1I	GCAGC	3 cut(s)	311, 634, 1188
BstV2I	GAAGAC	2 cut(s)	544, 685
BstX2I	RGATCY	5 cut(s)	1127, 1656, 1685, 1794, 2109
BstYI	RGATCY	5 cut(s)	1127, 1656, 1685, 1794, 2109
BstZI	CGGCCG	2 cut(s)	2294, 2306
Bsu15I	ATCGAT	3 cut(s)	670, 1761, 1829
BsuRI	GGCC	7 cut(s)	358, 663, 799, 991, 1410, 2296, 2308
BsuTUI	ATCGAT	3 cut(s)	670, 1761, 1829
BtgI	CCRYGG	3 cut(s)	101, 784, 1996
BtgZI	GCGATG	1 cut(s)	2288
BtrI	CACGTC	2 cut(s)	1215, 2053
BtsCI	GGATG	8 cut(s)	21, 36, 110, 1197, 1605, 1989, 2073, 2265
BtsI	GCAGTG	1 cut(s)	1870
BtsIMutI	CAGTG	3 cut(s)	453, 561, 1870
BveI	ACCTGC	1 cut(s)	57
CaiI	CAGNNNCTG	2 cut(s)	601, 1011
CfoI	GCGC	4 cut(s)	638, 1521, 1640, 2011
Cfr10I	RCCGGY	4 cut(s)	158, 302, 872, 1553
Cfr42I	CCGCGG	1 cut(s)	1999
ClaI	ATCGAT	3 cut(s)	670, 1761, 1829
CseI	GACGC	5 cut(s)	295, 517, 802, 997, 1571
CviAII	CATG	7 cut(s)	102, 481, 1054, 1369, 1547, 1966, 2005
DdeI	CTNAG	4 cut(s)	167, 202, 1660, 2387
DinI	GGCGCC	2 cut(s)	637, 2010
DrdI	GACNNNNNNGTC	1 cut(s)	1174
DriI	GACNNNNNGTC	2 cut(s)	1215, 1383
DseDI	GACNNNNNNGTC	1 cut(s)	1174
EaeI	YGGCCR	3 cut(s)	356, 2294, 2306
EagI	CGGCCG	2 cut(s)	2294, 2306
Eam1104I	CTCTTC	3 cut(s)	197, 331, 1709
Eam1105I	GACNNNNNGTC	2 cut(s)	1215, 1383
EarI	CTCTTC	3 cut(s)	197, 331, 1709
EciI	GGCGGA	9 cut(s)	52, 214, 319, 859, 891, 1233, 1319, 1592, 2042
Ecl136II	GAGCTC	1 cut(s)	831
EclXI	CGGCCG	2 cut(s)	2294, 2306
Eco130I	CCWWGG	3 cut(s)	101, 2043, 2113
Eco24I	GRGCYC	4 cut(s)	608, 833, 1412, 2364
Eco31I	GGTCTC	1 cut(s)	1391
Eco32I	GATATC	3 cut(s)	1926, 1977, 2353
Eco47I	GGWCC	6 cut(s)	246, 362, 1992, 2029, 2095, 2203
Eco52I	CGGCCG	2 cut(s)	2294, 2306
Eco53kI	GAGCTC	1 cut(s)	831
Eco57I	CTGAAG	1 cut(s)	1970
Eco72I	CACGTG	1 cut(s)	792
Eco88I	CYCGRG	2 cut(s)	1094, 1388
EcoICRI	GAGCTC	1 cut(s)	831
EcoNI	CCTNNNNNAGG	1 cut(s)	1844
EcoO109I	RGGNCCY	3 cut(s)	362, 1408, 1992
EcoRII	CCWGG	2 cut(s)	358, 1026
EcoRV	GATATC	3 cut(s)	1926, 1977, 2353
EcoT14I	CCWWGG	3 cut(s)	101, 2043, 2113
EcoT38I	GRGCYC	4 cut(s)	608, 833, 1412, 2364
EgeI	GGCGCC	2 cut(s)	637, 2010
EheI	GGCGCC	2 cut(s)	637, 2010
ErhI	CCWWGG	3 cut(s)	101, 2043, 2113
Esp3I	CGTCTC	3 cut(s)	320, 1202, 1482
FaeI	CATG	7 cut(s)	105, 484, 1057, 1372, 1550, 1969, 2008
FalI	AAGNNNNNCTT	2 cut(s)	1688, 1720
FaqI	GGGAC	4 cut(s)	1203, 1661, 2005, 2356
FatI	CATG	7 cut(s)	101, 480, 1053, 1368, 1546, 1965, 2004
FauI	CCCGC	8 cut(s)	858, 962, 1000, 1245, 1408, 1472, 1991, 2003
FblI	GTMKAC	2 cut(s)	570, 1155
FokI	GGATG	8 cut(s)	8, 23, 97, 1204, 1612, 1996, 2080, 2272
FriOI	GRGCYC	4 cut(s)	608, 833, 1412, 2364
FspBI	CTAG	1 cut(s)	2398
GlaI	GCGC	4 cut(s)	637, 1520, 1639, 2010
GsaI	CCCAGC	1 cut(s)	118
GsuI	CTGGAG	2 cut(s)	1073, 1226
HaeII	RGCGCY	2 cut(s)	639, 2012
HaeIII	GGCC	7 cut(s)	358, 663, 799, 991, 1410, 2296, 2308
HgaI	GACGC	5 cut(s)	295, 517, 802, 997, 1571
HhaI	GCGC	4 cut(s)	638, 1521, 1640, 2011
Hin1I	GRCGYC	5 cut(s)	306, 567, 636, 1563, 2009
Hin1II	CATG	7 cut(s)	105, 484, 1057, 1372, 1550, 1969, 2008
Hin6I	GCGC	4 cut(s)	636, 1519, 1638, 2009
HinP1I	GCGC	4 cut(s)	636, 1519, 1638, 2009
HincII	GTYRAC	4 cut(s)	535, 1156, 1509, 1652
HindII	GTYRAC	4 cut(s)	535, 1156, 1509, 1652
HpaI	GTTAAC	1 cut(s)	1509
HphI	GGTGA	7 cut(s)	490, 574, 1042, 1571, 1981, 2098, 2263
Hpy166II	GTNNAC	9 cut(s)	6, 535, 571, 784, 1156, 1495, 1509, 1652, 2056
Hpy8I	GTNNAC	9 cut(s)	6, 535, 571, 784, 1156, 1495, 1509, 1652, 2056
HpyAV	CCTTC	8 cut(s)	19, 43, 91, 375, 457, 512, 605, 2314
HpyCH4III	ACNGT	4 cut(s)	463, 824, 2207, 2347
HpyCH4IV	ACGT	8 cut(s)	567, 791, 954, 1214, 1491, 1674, 2052, 2187
HpyCH4V	TGCA	8 cut(s)	622, 1290, 1603, 1870, 1987, 2239, 2266, 2287
HpyF10VI	GCNNNNNNNGC	8 cut(s)	278, 525, 612, 635, 1945, 2293, 2302, 2368
HpyF3I	CTNAG	4 cut(s)	167, 202, 1660, 2387
HpySE526I	ACGT	8 cut(s)	567, 791, 954, 1214, 1491, 1674, 2052, 2187
Hsp92I	GRCGYC	5 cut(s)	306, 567, 636, 1563, 2009
Hsp92II	CATG	7 cut(s)	105, 484, 1057, 1372, 1550, 1969, 2008
HspAI	GCGC	4 cut(s)	636, 1519, 1638, 2009
KasI	GGCGCC	2 cut(s)	635, 2008
KflI	GGGWCCC	1 cut(s)	1992
KpnI	GGTACC	1 cut(s)	214
KroI	GCCGGC	3 cut(s)	302, 872, 1553
KroNI	GCCGGC	3 cut(s)	304, 874, 1555
KspAI	GTTAAC	1 cut(s)	1509
KspI	CCGCGG	1 cut(s)	1999
LmnI	GCTCC	7 cut(s)	488, 611, 836, 1092, 1198, 1306, 2359
Lsp1109I	GCAGC	3 cut(s)	311, 634, 1188
LweI	GCATC	5 cut(s)	1013, 1590, 1974, 2236, 2292
MaeI	CTAG	1 cut(s)	2398
MaeII	ACGT	8 cut(s)	567, 791, 954, 1214, 1491, 1674, 2052, 2187
MaeIII	GTNAC	4 cut(s)	1559, 1582, 1720, 1969
MbiI	CCGCTC	2 cut(s)	943, 1771
MfeI	CAATTG	2 cut(s)	985, 1039
MflI	RGATCY	5 cut(s)	1127, 1656, 1685, 1794, 2109
MhlI	GDGCHC	6 cut(s)	608, 833, 851, 1089, 1412, 2364
MlsI	TGGCCA	1 cut(s)	358
MluNI	TGGCCA	1 cut(s)	358
Mly113I	GGCGCC	2 cut(s)	636, 2009
MlyI	GAGTC	6 cut(s)	89, 100, 387, 1227, 1372, 1727
MmeI	TCCRAC	4 cut(s)	1732, 2058, 2189, 2316
Mox20I	TGGCCA	1 cut(s)	358
MreI	CGCCGGCG	2 cut(s)	872, 1553
MroNI	GCCGGC	3 cut(s)	302, 872, 1553
MscI	TGGCCA	1 cut(s)	358
MseI	TTAA	6 cut(s)	419, 909, 1437, 1508, 1533, 2175
MslI	CAYNNNNRTG	1 cut(s)	2244
Msp20I	TGGCCA	1 cut(s)	358
MspA1I	CMGCKG	2 cut(s)	338, 1998
MspR9I	CCNGG	9 cut(s)	360, 763, 855, 1028, 1125, 1151, 1405, 1597, 1713
MunI	CAATTG	2 cut(s)	985, 1039
MvaI	CCWGG	2 cut(s)	360, 1028
MvnI	CGCG	6 cut(s)	539, 1262, 1521, 1640, 1835, 1998
MwoI	GCNNNNNNNGC	8 cut(s)	278, 525, 612, 635, 1945, 2293, 2302, 2368
NaeI	GCCGGC	3 cut(s)	304, 874, 1555
NarI	GGCGCC	2 cut(s)	636, 2009
NciI	CCSGG	7 cut(s)	763, 855, 1125, 1151, 1405, 1597, 1713
NcoI	CCATGG	1 cut(s)	101
NgoMIV	GCCGGC	3 cut(s)	302, 872, 1553
NlaIII	CATG	7 cut(s)	105, 484, 1057, 1372, 1550, 1969, 2008
NmeAIII	GCCGAG	1 cut(s)	1591
NmuCI	GTSAC	2 cut(s)	1559, 1969
NspI	RCATGY	1 cut(s)	1550
NspV	TTCGAA	2 cut(s)	374, 1256
PaeR7I	CTCGAG	2 cut(s)	1094, 1388
PcsI	WCGNNNNNNNCGW	6 cut(s)	797, 810, 894, 971, 1262, 2133
PdiI	GCCGGC	3 cut(s)	304, 874, 1555
PfeI	GAWTC	8 cut(s)	577, 667, 866, 879, 1016, 1050, 1253, 2254
Pfl23II	CGTACG	2 cut(s)	1425, 2223
PflMI	CCANNNNNTGG	1 cut(s)	601
Ple19I	CGATCG	2 cut(s)	690, 1833
PleI	GAGTC	6 cut(s)	89, 100, 387, 1227, 1372, 1727
PluTI	GGCGCC	2 cut(s)	639, 2012
PmaCI	CACGTG	1 cut(s)	792
PmlI	CACGTG	1 cut(s)	792
PpsI	GAGTC	6 cut(s)	89, 100, 387, 1227, 1372, 1727
Ppu21I	YACGTR	2 cut(s)	792, 955
PpuMI	RGGWCCY	2 cut(s)	362, 1992
PshAI	GACNNNNGTC	1 cut(s)	729
PsiI	TTATAA	1 cut(s)	1343
Psp124BI	GAGCTC	1 cut(s)	833
Psp5II	RGGWCCY	2 cut(s)	362, 1992
Psp6I	CCWGG	2 cut(s)	358, 1026
PspCI	CACGTG	1 cut(s)	792
PspFI	CCCAGC	1 cut(s)	114
PspGI	CCWGG	2 cut(s)	358, 1026
PspLI	CGTACG	2 cut(s)	1425, 2223
PspOMI	GGGCCC	1 cut(s)	1408
PspPPI	RGGWCCY	2 cut(s)	362, 1992
PstNI	CAGNNNCTG	2 cut(s)	601, 1011
PsuI	RGATCY	5 cut(s)	1127, 1656, 1685, 1794, 2109
PvuI	CGATCG	2 cut(s)	690, 1833
RseI	CAYNNNNRTG	1 cut(s)	2244
SacI	GAGCTC	1 cut(s)	833
SacII	CCGCGG	1 cut(s)	1999
SalI	GTCGAC	1 cut(s)	1154
SaqAI	TTAA	6 cut(s)	419, 909, 1437, 1508, 1533, 2175
SchI	GAGTC	6 cut(s)	89, 100, 387, 1227, 1372, 1727
ScrFI	CCNGG	9 cut(s)	360, 763, 855, 1028, 1125, 1151, 1405, 1597, 1713
SduI	GDGCHC	6 cut(s)	608, 833, 851, 1089, 1412, 2364
SfaNI	GCATC	5 cut(s)	1013, 1590, 1974, 2236, 2292
SfoI	GGCGCC	2 cut(s)	637, 2010
Sfr274I	CTCGAG	2 cut(s)	1094, 1388
Sfr303I	CCGCGG	1 cut(s)	1999
SfuI	TTCGAA	2 cut(s)	374, 1256
SgrAI	CRCCGGYG	2 cut(s)	872, 1553
SgrBI	CCGCGG	1 cut(s)	1999
SinI	GGWCC	6 cut(s)	246, 362, 1992, 2029, 2095, 2203
SlaI	CTCGAG	2 cut(s)	1094, 1388
SmiMI	CAYNNNNRTG	1 cut(s)	2244
SmlI	CTYRAG	2 cut(s)	1094, 1388
SmoI	CTYRAG	2 cut(s)	1094, 1388
SspDI	GGCGCC	2 cut(s)	635, 2008
SspMI	CTAG	1 cut(s)	2398
SstI	GAGCTC	1 cut(s)	833
StyD4I	CCNGG	9 cut(s)	358, 761, 853, 1026, 1123, 1149, 1403, 1595, 1711
StyI	CCWWGG	3 cut(s)	101, 2043, 2113
TaaI	ACNGT	4 cut(s)	463, 824, 2207, 2347
TaiI	ACGT	8 cut(s)	570, 794, 957, 1217, 1494, 1677, 2055, 2190
TatI	WGTACW	3 cut(s)	1350, 1359, 2055
TauI	GCSGC	8 cut(s)	158, 335, 338, 739, 946, 1304, 1445, 2308
TfiI	GAWTC	8 cut(s)	577, 667, 866, 879, 1016, 1050, 1253, 2254
Tru1I	TTAA	6 cut(s)	419, 909, 1437, 1508, 1533, 2175
Tru9I	TTAA	6 cut(s)	419, 909, 1437, 1508, 1533, 2175
TscAI	CASTG	3 cut(s)	460, 568, 1877
TseFI	GTSAC	2 cut(s)	1559, 1969
TseI	GCWGC	3 cut(s)	299, 622, 1201
Tsp45I	GTSAC	2 cut(s)	1559, 1969
TspDTI	ATGAA	3 cut(s)	25, 1042, 2198
TspGWI	ACGGA	7 cut(s)	226, 302, 426, 903, 1211, 1669, 2372
TspRI	CASTG	3 cut(s)	460, 568, 1877
Van91I	CCANNNNNTGG	1 cut(s)	601
VpaK11BI	GGWCC	6 cut(s)	246, 362, 1992, 2029, 2095, 2203
XagI	CCTNNNNNAGG	1 cut(s)	1844
XapI	RAATTY	5 cut(s)	703, 711, 919, 958, 1180
XceI	RCATGY	1 cut(s)	1550
XcmI	CCANNNNNNNNNTGG	1 cut(s)	791
XhoI	CTCGAG	2 cut(s)	1094, 1388
XmiI	GTMKAC	2 cut(s)	570, 1155
XspI	CTAG	1 cut(s)	2398
ZraI	GACGTC	1 cut(s)	568

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.