Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0015839

Physical Location & Seq

Genomic Coordinates Forward (+)

1314819 .. 1317717

Gene Structure 2899 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1018000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1899 bp

ATGGCTATGGCGAACAAATACGAAGAAGAGGAGGAGGAGGAGGTGCAGAGCTTTGGAAAGCGGCTGAAATGGAGCAGATACGAGGAGCCTCTGGAGGGGGAGGAGGAAGAAGAGGAGGACGACGACGACGAGAGCCAGCTCGCTCTGAAGCCGTGGACGACGGCGTTCCCAATGAGGCCGGCGTCGTTTGTGGTGTCGGATGCTCTGGAGCCCGACAGTCCTATCATTTACGTCAACAAAGTCTTTGAGACCTTCACCGGCTATTGTGCCCACGAGGTCCTCGGCCGAAACTGTCGATTCCTGCAATATAGGGACCCTCATGCTCAAAGGCGGCATCCTCTTGTAGATCCAGTTGTGGTTTCGGAGATTCGAAGGTGTCTCGAGGAAGGAGTTGAGTTTCACGGAGAGCTTCTTAATTTCAGTAAGGATGGAACTCCCTTGGTGAATAGACTAAGACTGAAACCTATACACGATGATAACGGAACCGTCACTCACATCATAGGCATTCAAGTATTTTCTGAAGCAAAAATAGATCTCAACAGTGTATCATATCCAGTTTACAAACAGACATGCAATCAACAGTCTGATCAATCTGGTAAATATCCTCTCATCAGTGGACAAACACCTTTCACCCAGTATCACGATATATGCGGGATTCTTCAGCTCTCTGATGAAGTTTTGGCTCACAACATTTTGTCACGCTTGACTCCGAGGGATGTGGCATCCATTGGGTCTGTGTGCAGAAGAATCCGCCAACTGACAAAAAATGAGCATGTGAGGAAGATGGTATGCCAAAATGCATGGGGAAGAGAAGTCACCGGTACATTGGAACTCATGACTAATAAGTTAGGGTGGGGGCGTCTGGCTAGGGAACTGACAACCCTTGAGGCCGTTTCTTGGAGGAAATTTACGGTTGGAGGTGCCGTGGAGCCTTCAAGATGCAATTTTGGTGCTTGTGCTGTAGGAAACCGACTTGTGCTATTTGGAGGTGAAGGAGTTGATATGCAGCCAATGAACGACACATTTGTTCTCAATCTTGATGCTGCCAATCCTGAGTGGTGTCGAGTAAGAGTGAAATCGTCGCCACCAGGGCGTTGGGGCCACACCCTTTCATGGTTGAATGGTTCTTGGTTGGTTGTATTTGGAGGCTGTGGGCGGGAAGGATTGCTCAATGATGTGTTCGTTCTTGACTTGGATGCCAAGCAGCCAACGTGGAAAGAAGTTTTTGGTGGAACTCCCCCTCCTCCTAGATCCTGGCACAGCTCTTGCACATTAGAAGGTTCGAAATTGGTTGTGTCGGGTGGATGCACGGATGCTGGGGTCCTTCTTAGTGACACGTACTTACTGGATCTGGCAACAGACAACCCAAGATGGAGAGAGATTCCAACTTCATGGGCTCCTCCTTCTAGGTTGGGGCATTCCCTCTCTGTTTATGGTCGGTCAAAGATTCTCATGTTTGGTGGACTGGCCAACAGTGGGCACTTGCGGTTACGATCAGGGGAGACTTACACCATCGATTTGGAAGATGAAAACCCCCAGTGGAGGCAACTGGAGTGTAATGCGTTCACCAGCATGGGCAGCCAGAGTGCAGTGGTTCCCCCTCCCAGACTTGACCATGTTGCAGTCAGCATGCCATGCGGAAGGATTATAATATTTGGTGGTTCAATCGCGGGGCTGCATTCTCCTTCTCAGCTTTTCCTCTTGGATCCTTCGGAAGAGAAACCATCATGGAGGATTCTCAACGTTCCGGGGCAACCACCTAAATTTGCTTGGGGCCATAGCACTTGCGTGGTCGGAGGAACGAGGGTCCTGGTATTGGGTGGGCACACAGGTGAAGAGTGGATACTTAATGACTTGTATGAGTTGCGCTTAGCAAGCAAGCAGGATTCAGACCACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0007623 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009637 GO:0009889 GO:0009909 GO:0009911 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048511 GO:0048518 GO:0048580 GO:0048582 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051239 GO:0051240 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04712 map04712

Pfam Domains

Protein Families

F-box Kelch_2 Kelch_4 PAS_9

Protein Analysis

633

Amino Acids

70.55

Weight (kDa)

5.53

Isoelectric Point (pI)

49.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAS_9	PF13426	69 - 170	6.4e-15	PAS domain
PAS_3	PF08447	75 - 169	1.3e-07	PAS fold
Kelch_KLHDC2_KLHL20_DRC7	PF24681	294 - 488	1.1e-37	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42	PF13415	296 - 454	8.1e-25	FBX42, beta-propeller domain
Beta-prop_TYW4	PF13418	312 - 555	4.4e-09	tRNA wybutosine-synthesizing protein 4, beta-propeller
Beta-prop_ATRN-LZTR1	PF24981	327 - 519	2.5e-14	Attractin/LZTR1 beta-propeller
Kelch_HCF	PF13854	351 - 608	1.3e-16	Host cell factor, Kelch-repeats domain
Kelch_KLHDC2_KLHL20_DRC7	PF24681	405 - 521	1.6e-16	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42	PF13415	454 - 554	2.4e-08	FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7	PF24681	472 - 628	1.6e-17	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1	PF24981	521 - 627	2.1e-06	Attractin/LZTR1 beta-propeller
Beta-prop_FBX42	PF13415	529 - 627	3.8e-12	FBX42, beta-propeller domain
Kelch_2	PF07646	537 - 584	1.7e-07	Kelch motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0015839)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68050
fragaria_vesca	FvH4_4g33490
malus_domestica	MD16G1018000.v1.1
prunus_persica	Prupe.1G334000_v2.0.a1
pyrus_communis	pycom16g01630
rosa_chinensis	RchiOBHm_Chr4g0442801
rosa_laevigata	RLG00000005983
rosa_multiflora	Rmu_ssc0000204.1_g000019
rosa_roxburghii	Rroxscaffold_5G00383580
rosa_rugosa	Rorug04G0343300
rosa_samantha	Rh4AG395800 Rh4BG408700 Rh4CG424300 Rh4DG402800
rosa_wichuraiana	Rw4G034210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1649
AccB1I	GGYRCC	1 cut(s)	920
AccII	CGCG	1 cut(s)	1670
AciI	CCGC	8 cut(s)	61, 331, 651, 751, 1156, 1486, 1638, 1670
AclI	AACGTT	1 cut(s)	1743
AclWI	GGATC	5 cut(s)	341, 1245, 1356, 1700, 1713
AcoI	YGGCCR	2 cut(s)	283, 1467
AcsI	RAATTY	2 cut(s)	905, 1763
AcuI	CTGAAG	3 cut(s)	167, 540, 644
AcyI	GRCGYC	2 cut(s)	182, 859
AfaI	GTAC	2 cut(s)	823, 1340
AfiI	CCNNNNNNNGG	6 cut(s)	95, 897, 1113, 1476, 1542, 1816
AflIII	ACRYGT	1 cut(s)	1335
AgeI	ACCGGT	1 cut(s)	818
AgsI	TTSAA	4 cut(s)	509, 936, 1120, 1665
AjnI	CCWGG	3 cut(s)	1087, 1253, 1809
AleI	CACNNNNGTG	2 cut(s)	1787, 1830
AloI	GAACNNNNNNTCC	2 cut(s)	1225, 1257
AluBI	AGCT	6 cut(s)	51, 139, 409, 664, 1263, 1693
AluI	AGCT	6 cut(s)	51, 139, 409, 664, 1263, 1693
Alw26I	GTCTC	3 cut(s)	242, 383, 1496
AlwI	GGATC	5 cut(s)	341, 1245, 1356, 1700, 1713
Ama87I	CYCGRG	1 cut(s)	380
AoxI	GGCC	6 cut(s)	176, 283, 888, 1099, 1467, 1774
ApeKI	GCWGC	5 cut(s)	1006, 1043, 1204, 1578, 1675
ApoI	RAATTY	2 cut(s)	905, 1763
AsiGI	ACCGGT	1 cut(s)	818
AspLEI	GCGC	1 cut(s)	1869
AspS9I	GGNCC	6 cut(s)	277, 313, 1099, 1321, 1774, 1807
AsuC2I	CCSGG	1 cut(s)	1749
AsuHPI	GGTGA	7 cut(s)	247, 454, 622, 808, 1001, 1558, 1844
AsuII	TTCGAA	2 cut(s)	370, 1283
AvaI	CYCGRG	1 cut(s)	380
AvaII	GGWCC	4 cut(s)	277, 313, 1321, 1807
BaeGI	GKGCMC	3 cut(s)	271, 1482, 1827
BalI	TGGCCA	1 cut(s)	1469
BamHI	GGATCC	1 cut(s)	1705
BanI	GGYRCC	1 cut(s)	920
BanII	GRGCYC	2 cut(s)	213, 1399
BarI	GAAGNNNNNNTAC	2 cut(s)	1827, 1859
BauI	CACGAG	1 cut(s)	272
BbvI	GCAGC	5 cut(s)	1018, 1030, 1216, 1590, 1662
BccI	CCATC	5 cut(s)	422, 778, 1365, 1520, 1732
BceAI	ACGGC	4 cut(s)	136, 177, 875, 908
BciT130I	CCWGG	3 cut(s)	1089, 1255, 1811
BciVI	GTATCC	1 cut(s)	1836
BclI	TGATCA	1 cut(s)	586
BcnI	CCSGG	1 cut(s)	1749
BcoDI	GTCTC	3 cut(s)	242, 383, 1496
BfaI	CTAG	3 cut(s)	867, 1248, 1407
BfmI	CTRYAG	1 cut(s)	960
BfuI	GTATCC	1 cut(s)	1836
BglI	GCCNNNNNGGC	1 cut(s)	1090
BglII	AGATCT	1 cut(s)	532
BisI	GCNGC	7 cut(s)	62, 332, 1007, 1044, 1205, 1579, 1676
BlpI	GCTNAGC	1 cut(s)	1870
BlsI	GCNGC	7 cut(s)	63, 333, 1008, 1045, 1206, 1580, 1677
Bme1390I	CCNGG	4 cut(s)	1089, 1255, 1749, 1811
Bme18I	GGWCC	4 cut(s)	277, 313, 1321, 1807
BmeT110I	CYCGRG	1 cut(s)	380
BmgT120I	GGNCC	6 cut(s)	277, 313, 1099, 1321, 1774, 1807
BmrFI	CCNGG	4 cut(s)	1089, 1255, 1749, 1811
BmrI	ACTGGG	2 cut(s)	628, 1531
BmsI	GCATC	8 cut(s)	190, 343, 731, 929, 1030, 1186, 1295, 1303
BmuI	ACTGGG	2 cut(s)	628, 1531
BpmI	CTGGAG	3 cut(s)	113, 227, 1571
Bpu1102I	GCTNAGC	1 cut(s)	1870
Bpu14I	TTCGAA	2 cut(s)	370, 1283
BpuEI	CTTGAG	1 cut(s)	905
BpuMI	CCSGG	1 cut(s)	1749
Bsa29I	ATCGAT	1 cut(s)	1515
BsaAI	YACGTR	1 cut(s)	1338
BsaHI	GRCGYC	2 cut(s)	182, 859
BsaI	GGTCTC	1 cut(s)	242
BsaJI	CCNNGG	7 cut(s)	152, 280, 438, 710, 924, 1088, 1748
BsaWI	WCCGGW	1 cut(s)	818
BsaXI	ACNNNNNCTCC	4 cut(s)	909, 939, 1225, 1255
Bsc4I	CCNNNNNNNGG	6 cut(s)	95, 897, 1113, 1476, 1542, 1816
Bse118I	RCCGGY	3 cut(s)	178, 257, 818
Bse1I	ACTGG	7 cut(s)	350, 554, 634, 1350, 1470, 1537, 1554
BseBI	CCWGG	3 cut(s)	1089, 1255, 1811
BseCI	ATCGAT	1 cut(s)	1515
BseDI	CCNNGG	7 cut(s)	152, 280, 438, 710, 924, 1088, 1748
BseGI	GGATG	8 cut(s)	205, 334, 433, 721, 722, 1201, 1310, 1318
BseLI	CCNNNNNNNGG	6 cut(s)	95, 897, 1113, 1476, 1542, 1816
BseMII	CTCAG	2 cut(s)	1044, 1703
BseNI	ACTGG	7 cut(s)	350, 554, 634, 1350, 1470, 1537, 1554
BseRI	GAGGAG	9 cut(s)	44, 47, 50, 53, 98, 116, 128, 1233, 1389
BseSI	GKGCMC	3 cut(s)	271, 1482, 1827
BseX3I	CGGCCG	1 cut(s)	283
BseXI	GCAGC	5 cut(s)	1018, 1030, 1216, 1590, 1662
BseYI	CCCAGC	1 cut(s)	1316
BsgI	GTGCAG	3 cut(s)	65, 760, 1608
Bsh1236I	CGCG	1 cut(s)	1670
Bsh1285I	CGRYCG	1 cut(s)	286
BshFI	GGCC	6 cut(s)	178, 285, 890, 1101, 1469, 1776
BshNI	GGYRCC	1 cut(s)	920
BshTI	ACCGGT	1 cut(s)	818
BshVI	ATCGAT	1 cut(s)	1515
BsiEI	CGRYCG	1 cut(s)	286
BsiHKCI	CYCGRG	1 cut(s)	380
BsiSI	CCGG	4 cut(s)	179, 258, 819, 1748
BslFI	GGGAC	1 cut(s)	326
BslI	CCNNNNNNNGG	6 cut(s)	95, 897, 1113, 1476, 1542, 1816
BsmAI	GTCTC	3 cut(s)	242, 383, 1496
BsmFI	GGGAC	1 cut(s)	326
BsmI	GAATGC	3 cut(s)	504, 1417, 1678
BsnI	GGCC	6 cut(s)	178, 285, 890, 1101, 1469, 1776
Bso31I	GGTCTC	1 cut(s)	242
BsoBI	CYCGRG	1 cut(s)	380
Bsp119I	TTCGAA	2 cut(s)	370, 1283
Bsp1286I	GDGCHC	5 cut(s)	213, 271, 1399, 1482, 1827
Bsp143I	GATC	7 cut(s)	346, 532, 586, 1250, 1348, 1493, 1705
Bsp1720I	GCTNAGC	1 cut(s)	1870
BspACI	CCGC	8 cut(s)	61, 331, 651, 751, 1156, 1486, 1638, 1670
BspANI	GGCC	6 cut(s)	178, 285, 890, 1101, 1469, 1776
BspCNI	CTCAG	2 cut(s)	1045, 1702
BspDI	ATCGAT	1 cut(s)	1515
BspFNI	CGCG	1 cut(s)	1670
BspHI	TCATGA	1 cut(s)	834
BspPI	GGATC	5 cut(s)	341, 1245, 1356, 1700, 1713
BspT104I	TTCGAA	2 cut(s)	370, 1283
BspT107I	GGYRCC	1 cut(s)	920
BspTNI	GGTCTC	1 cut(s)	242
BsrFI	RCCGGY	3 cut(s)	178, 257, 818
BsrI	ACTGG	7 cut(s)	350, 554, 634, 1350, 1470, 1537, 1554
BssAI	RCCGGY	3 cut(s)	178, 257, 818
BssECI	CCNNGG	7 cut(s)	152, 280, 438, 710, 924, 1088, 1748
BssMI	GATC	7 cut(s)	346, 532, 586, 1250, 1348, 1493, 1705
BssNI	GRCGYC	2 cut(s)	182, 859
BssSI	CACGAG	1 cut(s)	272
BssT1I	CCWWGG	1 cut(s)	438
Bst2BI	CACGAG	1 cut(s)	272
Bst2UI	CCWGG	3 cut(s)	1089, 1255, 1811
Bst4CI	ACNGT	7 cut(s)	218, 293, 487, 542, 582, 913, 1475
Bst6I	CTCTTC	5 cut(s)	21, 105, 802, 1710, 1830
BstACI	GRCGYC	2 cut(s)	182, 859
BstAPI	GCANNNNNTGC	1 cut(s)	1635
BstBAI	YACGTR	1 cut(s)	1338
BstBI	TTCGAA	2 cut(s)	370, 1283
BstC8I	GCNNGC	6 cut(s)	137, 141, 180, 1631, 1876, 1880
BstDEI	CTNAG	5 cut(s)	452, 1053, 1328, 1689, 1870
BstDSI	CCRYGG	2 cut(s)	152, 924
BstF5I	GGATG	8 cut(s)	205, 334, 433, 721, 722, 1201, 1310, 1318
BstFNI	CGCG	1 cut(s)	1670
BstHHI	GCGC	1 cut(s)	1869
BstKTI	GATC	7 cut(s)	349, 535, 589, 1253, 1351, 1496, 1708
BstMAI	GTCTC	3 cut(s)	242, 383, 1496
BstMBI	GATC	7 cut(s)	346, 532, 586, 1250, 1348, 1493, 1705
BstMCI	CGRYCG	1 cut(s)	286
BstMWI	GCNNNNNNNGC	4 cut(s)	1090, 1578, 1635, 1875
BstNI	CCWGG	3 cut(s)	1089, 1255, 1811
BstNSI	RCATGY	3 cut(s)	573, 776, 1633
BstSCI	CCNGG	4 cut(s)	1087, 1253, 1747, 1809
BstSFI	CTRYAG	1 cut(s)	960
BstSLI	GKGCMC	3 cut(s)	271, 1482, 1827
BstUI	CGCG	1 cut(s)	1670
BstV1I	GCAGC	5 cut(s)	1018, 1030, 1216, 1590, 1662
BstX2I	RGATCY	5 cut(s)	346, 532, 1250, 1348, 1705
BstXI	CCANNNNNNTGG	3 cut(s)	1095, 1392, 1519
BstYI	RGATCY	5 cut(s)	346, 532, 1250, 1348, 1705
BstZI	CGGCCG	1 cut(s)	283
Bsu15I	ATCGAT	1 cut(s)	1515
BsuI	GTATCC	1 cut(s)	1836
BsuRI	GGCC	6 cut(s)	178, 285, 890, 1101, 1469, 1776
BsuTUI	ATCGAT	1 cut(s)	1515
BtgI	CCRYGG	2 cut(s)	152, 924
BtsCI	GGATG	8 cut(s)	205, 334, 433, 721, 722, 1201, 1310, 1318
BtsI	GCAGTG	1 cut(s)	1596
BtsIMutI	CAGTG	6 cut(s)	547, 619, 1480, 1544, 1596, 1894
Cac8I	GCNNGC	6 cut(s)	137, 141, 180, 1631, 1876, 1880
CciI	TCATGA	1 cut(s)	834
CfoI	GCGC	1 cut(s)	1869
Cfr10I	RCCGGY	3 cut(s)	178, 257, 818
Cfr13I	GGNCC	6 cut(s)	277, 313, 1099, 1321, 1774, 1807
ClaI	ATCGAT	1 cut(s)	1515
CseI	GACGC	2 cut(s)	171, 848
Csp6I	GTAC	2 cut(s)	822, 1339
CspAI	ACCGGT	1 cut(s)	818
CviQI	GTAC	2 cut(s)	822, 1339
DdeI	CTNAG	5 cut(s)	452, 1053, 1328, 1689, 1870
DpnI	GATC	7 cut(s)	348, 534, 588, 1252, 1350, 1495, 1707
DpnII	GATC	7 cut(s)	346, 532, 586, 1250, 1348, 1493, 1705
EaeI	YGGCCR	2 cut(s)	283, 1467
EagI	CGGCCG	1 cut(s)	283
Eam1104I	CTCTTC	5 cut(s)	21, 105, 802, 1710, 1830
EarI	CTCTTC	5 cut(s)	21, 105, 802, 1710, 1830
EciI	GGCGGA	1 cut(s)	740
EclXI	CGGCCG	1 cut(s)	283
Eco130I	CCWWGG	1 cut(s)	438
Eco24I	GRGCYC	2 cut(s)	213, 1399
Eco31I	GGTCTC	1 cut(s)	242
Eco47I	GGWCC	4 cut(s)	277, 313, 1321, 1807
Eco52I	CGGCCG	1 cut(s)	283
Eco57I	CTGAAG	3 cut(s)	167, 540, 644
Eco88I	CYCGRG	1 cut(s)	380
EcoO109I	RGGNCCY	4 cut(s)	277, 313, 1321, 1807
EcoRII	CCWGG	3 cut(s)	1087, 1253, 1809
EcoT14I	CCWWGG	1 cut(s)	438
EcoT22I	ATGCAT	1 cut(s)	802
EcoT38I	GRGCYC	2 cut(s)	213, 1399
ErhI	CCWWGG	1 cut(s)	438
FaqI	GGGAC	1 cut(s)	326
FauI	CCCGC	3 cut(s)	644, 1149, 1663
FbaI	TGATCA	1 cut(s)	586
Fnu4HI	GCNGC	7 cut(s)	62, 332, 1007, 1044, 1205, 1579, 1676
FokI	GGATG	8 cut(s)	212, 321, 440, 709, 728, 1208, 1317, 1325
FriOI	GRGCYC	2 cut(s)	213, 1399
Fsp4HI	GCNGC	7 cut(s)	62, 332, 1007, 1044, 1205, 1579, 1676
FspBI	CTAG	3 cut(s)	867, 1248, 1407
GlaI	GCGC	1 cut(s)	1868
GluI	GCNGC	7 cut(s)	62, 332, 1007, 1044, 1205, 1579, 1676
GsaI	CCCAGC	1 cut(s)	1320
GsuI	CTGGAG	3 cut(s)	113, 227, 1571
HaeIII	GGCC	6 cut(s)	178, 285, 890, 1101, 1469, 1776
HapII	CCGG	4 cut(s)	179, 258, 819, 1748
HgaI	GACGC	2 cut(s)	171, 848
HhaI	GCGC	1 cut(s)	1869
Hin1I	GRCGYC	2 cut(s)	182, 859
Hin6I	GCGC	1 cut(s)	1867
HinP1I	GCGC	1 cut(s)	1867
HincII	GTYRAC	1 cut(s)	235
HindII	GTYRAC	1 cut(s)	235
HinfI	GANTC	9 cut(s)	297, 367, 655, 706, 747, 1381, 1447, 1735, 1886
HpaII	CCGG	4 cut(s)	179, 258, 819, 1748
HphI	GGTGA	7 cut(s)	247, 454, 622, 808, 1001, 1558, 1844
Hpy166II	GTNNAC	6 cut(s)	156, 235, 559, 617, 1463, 1566
Hpy188III	TCNNGA	9 cut(s)	92, 206, 380, 641, 835, 936, 1037, 1052, 1187
Hpy8I	GTNNAC	6 cut(s)	156, 235, 559, 617, 1463, 1566
Hpy99I	CGWCG	6 cut(s)	125, 128, 131, 163, 187, 1084
HpyCH4III	ACNGT	7 cut(s)	218, 293, 487, 542, 582, 913, 1475
HpyCH4IV	ACGT	4 cut(s)	231, 1211, 1337, 1743
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1090, 1578, 1635, 1875
HpyF3I	CTNAG	5 cut(s)	452, 1053, 1328, 1689, 1870
HpySE526I	ACGT	4 cut(s)	231, 1211, 1337, 1743
Hsp92I	GRCGYC	2 cut(s)	182, 859
HspAI	GCGC	1 cut(s)	1867
KflI	GGGWCCC	1 cut(s)	313
KroI	GCCGGC	1 cut(s)	178
KroNI	GCCGGC	1 cut(s)	180
Ksp22I	TGATCA	1 cut(s)	586
Kzo9I	GATC	7 cut(s)	346, 532, 586, 1250, 1348, 1493, 1705
LmnI	GCTCC	5 cut(s)	72, 85, 208, 928, 1402
Lsp1109I	GCAGC	5 cut(s)	1018, 1030, 1216, 1590, 1662
LweI	GCATC	8 cut(s)	190, 343, 731, 929, 1030, 1186, 1295, 1303
MaeI	CTAG	3 cut(s)	867, 1248, 1407
MaeII	ACGT	4 cut(s)	231, 1211, 1337, 1743
MaeIII	GTNAC	5 cut(s)	487, 696, 814, 1331, 1488
MalI	GATC	7 cut(s)	348, 534, 588, 1252, 1350, 1495, 1707
MboI	GATC	7 cut(s)	346, 532, 586, 1250, 1348, 1493, 1705
MflI	RGATCY	5 cut(s)	346, 532, 1250, 1348, 1705
MhlI	GDGCHC	5 cut(s)	213, 271, 1399, 1482, 1827
MlsI	TGGCCA	1 cut(s)	1469
MluCI	AATT	5 cut(s)	415, 905, 943, 1286, 1763
MluNI	TGGCCA	1 cut(s)	1469
MlyI	GAGTC	1 cut(s)	700
MmeI	TCCRAC	4 cut(s)	177, 895, 1409, 1774
Mox20I	TGGCCA	1 cut(s)	1469
Mph1103I	ATGCAT	1 cut(s)	802
MroNI	GCCGGC	1 cut(s)	178
MscI	TGGCCA	1 cut(s)	1469
MseI	TTAA	2 cut(s)	414, 1848
MslI	CAYNNNNRTG	3 cut(s)	1571, 1787, 1830
Msp20I	TGGCCA	1 cut(s)	1469
MspI	CCGG	4 cut(s)	179, 258, 819, 1748
MspR9I	CCNGG	4 cut(s)	1089, 1255, 1749, 1811
Mva1269I	GAATGC	3 cut(s)	504, 1417, 1678
MvaI	CCWGG	3 cut(s)	1089, 1255, 1811
MvnI	CGCG	1 cut(s)	1670
MwoI	GCNNNNNNNGC	4 cut(s)	1090, 1578, 1635, 1875
NaeI	GCCGGC	1 cut(s)	180
NciI	CCSGG	1 cut(s)	1749
NdeII	GATC	7 cut(s)	346, 532, 586, 1250, 1348, 1493, 1705
NgoMIV	GCCGGC	1 cut(s)	178
NmeAIII	GCCGAG	1 cut(s)	261
NmuCI	GTSAC	4 cut(s)	487, 696, 814, 1331
NsiI	ATGCAT	1 cut(s)	802
NspI	RCATGY	3 cut(s)	573, 776, 1633
NspV	TTCGAA	2 cut(s)	370, 1283
OliI	CACNNNNGTG	2 cut(s)	1787, 1830
PaeI	GCATGC	1 cut(s)	1633
PaeR7I	CTCGAG	1 cut(s)	380
PagI	TCATGA	1 cut(s)	834
PcsI	WCGNNNNNNNCGW	1 cut(s)	126
PctI	GAATGC	3 cut(s)	504, 1417, 1678
PdiI	GCCGGC	1 cut(s)	180
PfeI	GAWTC	8 cut(s)	297, 367, 655, 747, 1381, 1447, 1735, 1886
PinAI	ACCGGT	1 cut(s)	818
PkrI	GCNGC	7 cut(s)	63, 333, 1008, 1045, 1206, 1580, 1677
PleI	GAGTC	1 cut(s)	700
PpsI	GAGTC	1 cut(s)	700
Ppu21I	YACGTR	1 cut(s)	1338
PpuMI	RGGWCCY	4 cut(s)	277, 313, 1321, 1807
PsiI	TTATAA	1 cut(s)	1649
Psp1406I	AACGTT	1 cut(s)	1743
Psp5II	RGGWCCY	4 cut(s)	277, 313, 1321, 1807
Psp6I	CCWGG	3 cut(s)	1087, 1253, 1809
PspFI	CCCAGC	1 cut(s)	1316
PspGI	CCWGG	3 cut(s)	1087, 1253, 1809
PspPI	GGNCC	6 cut(s)	277, 313, 1099, 1321, 1774, 1807
PspPPI	RGGWCCY	4 cut(s)	277, 313, 1321, 1807
PsuI	RGATCY	5 cut(s)	346, 532, 1250, 1348, 1705
RsaI	GTAC	2 cut(s)	823, 1340
RsaNI	GTAC	2 cut(s)	822, 1339
RseI	CAYNNNNRTG	3 cut(s)	1571, 1787, 1830
SaqAI	TTAA	2 cut(s)	414, 1848
SatI	GCNGC	7 cut(s)	62, 332, 1007, 1044, 1205, 1579, 1676
Sau3AI	GATC	7 cut(s)	346, 532, 586, 1250, 1348, 1493, 1705
Sau96I	GGNCC	6 cut(s)	277, 313, 1099, 1321, 1774, 1807
SchI	GAGTC	1 cut(s)	700
ScrFI	CCNGG	4 cut(s)	1089, 1255, 1749, 1811
SduI	GDGCHC	5 cut(s)	213, 271, 1399, 1482, 1827
SfaNI	GCATC	8 cut(s)	190, 343, 731, 929, 1030, 1186, 1295, 1303
SfcI	CTRYAG	1 cut(s)	960
Sfr274I	CTCGAG	1 cut(s)	380
SfuI	TTCGAA	2 cut(s)	370, 1283
SinI	GGWCC	4 cut(s)	277, 313, 1321, 1807
SlaI	CTCGAG	1 cut(s)	380
SmiMI	CAYNNNNRTG	3 cut(s)	1571, 1787, 1830
SmlI	CTYRAG	2 cut(s)	380, 884
SmoI	CTYRAG	2 cut(s)	380, 884
SphI	GCATGC	1 cut(s)	1633
Sse9I	AATT	5 cut(s)	415, 905, 943, 1286, 1763
SsiI	CCGC	8 cut(s)	61, 331, 651, 751, 1156, 1486, 1638, 1670
SspI	AATATT	1 cut(s)	1653
SspMI	CTAG	3 cut(s)	867, 1248, 1407
StyD4I	CCNGG	4 cut(s)	1087, 1253, 1747, 1809
StyI	CCWWGG	1 cut(s)	438
TaaI	ACNGT	7 cut(s)	218, 293, 487, 542, 582, 913, 1475
TaiI	ACGT	4 cut(s)	234, 1214, 1340, 1746
TaqI	TCGA	6 cut(s)	295, 370, 381, 1063, 1283, 1515
TaqII	GACCGA	1 cut(s)	1428
TasI	AATT	5 cut(s)	415, 905, 943, 1286, 1763
TauI	GCSGC	2 cut(s)	64, 334
TfiI	GAWTC	8 cut(s)	297, 367, 655, 747, 1381, 1447, 1735, 1886
Tru1I	TTAA	2 cut(s)	414, 1848
Tru9I	TTAA	2 cut(s)	414, 1848
TscAI	CASTG	5 cut(s)	547, 619, 1480, 1544, 1596
TseFI	GTSAC	4 cut(s)	487, 696, 814, 1331
TseI	GCWGC	5 cut(s)	1006, 1043, 1204, 1578, 1675
Tsp45I	GTSAC	4 cut(s)	487, 696, 814, 1331
TspDTI	ATGAA	5 cut(s)	687, 1028, 1101, 1380, 1542
TspGWI	ACGGA	3 cut(s)	417, 495, 1325
TspRI	CASTG	5 cut(s)	547, 619, 1480, 1544, 1596
VpaK11BI	GGWCC	4 cut(s)	277, 313, 1321, 1807
XapI	RAATTY	2 cut(s)	905, 1763
XceI	RCATGY	3 cut(s)	573, 776, 1633
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1092
XhoI	CTCGAG	1 cut(s)	380
XspI	CTAG	3 cut(s)	867, 1248, 1407
Zsp2I	ATGCAT	1 cut(s)	802

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.