Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0015839

Physical Location & Seq

Genomic Coordinates Forward (+)

61277198 .. 61279916

Gene Structure 2719 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G034210.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1896 bp

ATGGCCATGGCGAAGAAGCACGAAGGAGAAGAGGAGGAGGAGGAGGTTCAGAACTTCGGGAAGAGACTGAAATGCAGCAAATACGAGGAGGTTCGAGACGAATATGAAGAAGAAGAAGAAGAAGAGAGCGAGGTTTCAGAGAAGAGAGAGATGCTTAGCATGTACCCAATGACTCCCACATCGTTTGTGGTCTCCGATGCTCTGGAACCCGATTGTCCGATCATTTACGTCAACAAAGTGTTCGAGACGTTCACCGGATATGGAGCTCATGAAGTCCTCGGTCGGAACTGTCGGTTCTTACAATATAGAGACCCTCATGCTCAAAGACGACATCCTCTGGTAGATCCTGATGTCGTTTCTGAGATTAGAAGGTGTCTTGAAGAAGGAGTTGAATTCTTAGGTGAGCTTCTTAATTTCAGTAAGGATGGCACTCCCTTGGTGAATAGGCTAAGGCTGAAGCCTATACTTGATGATAACGGAACTGTCACACACATCATAGGCATTCAAGTATTTGCTGAAACAAAAATAGATCTCAATAGTGTGTCATATCCAGTTTATAAAGAGACATGCACTGAGCAGTTCACTCAGCCCGATAAATATTCTCTCACCAGTGGACAAACACCTTCCACCCAACATCAAGAAATTTGTGGGATTCTGCAGCTCTCGGATGAAGTCTTGGCTCACAACATCTTATCGCGCTTGACTCCAAGGGATGTGGCATCCATTGGGTCTGTCTGCAGAAGAATCCGTCAGCTAACGAAAAATGAGCATGTGAGGAAGATGGTATGTCAAAATGCATGGGGAAGAGAAGTCACTGGTACATTGGAACTGATGACTAAGAAGTTAGGGTGGGGGCGTCTGGCTAGGGAACTGACAACTCTTGAGGCTGTTTGTTGGAGGAAGTTTACTGTTGGAGGTTCCGTGGAGCCTTCACGGTGCAATTTCAGTGCTTGTGCTGTGGGAAATCGACTGGTATTGTTTGGAGGTGAAGGAGTGGATATGCAGCCAATGGATGACACATTTGTTCTCAATCTTGATGCTGCCAATCCAGAGTGGACTAGAGTAAGTGTGAAATCATCACCACCAGGTCGGTGGGGCCATACACTTTCATGTTTGAATGGTTCTTTGCTAGTAGTTTTTGGGGGATGTGGGCGGCAAGGGTTGCTCAATGATGTTTGGGTTCTTGACTTGGATGCCAAGCAGCCAACATGGAAAGAAGTTTATGGTGGAACTCCTCCTCTCCCTAGATCCTGGCATAGCTCTTGCATAACAGAAGGTTCTAAATTAGTTGTGTCAGGTGGATGCACGGATGCTGGGGTACTTCTTAGCGACACATACGTATTGGATCTCACCACTGACAATCCAACATGGAGAGAGATTCCAACTTCATGGTCTCCTCCCTCTAGGTTGGGACATTCCCTTTCAGTTTATGGCCGGTCAAAGATTCTCATGTTTGGTGGACTTGCCAACAGTGGGCACTTGCGGTTAAGATCAGGTGAGACTTACACCATTGATTTGGAAGACGAAGAGCCTCAATGGAGGCAACTGGAGTGTAATGCATTTACCAGCATTGACAGCCAGAGTGCGGTGCTCCCTCCTCCTAGACTTGATCATGTTGCTGTGAGCATGCCTTGCGGAAGGATTATCATTTTTGGTGGTTCAATTGCCGGGCTGCATTCTCCTTCTCAGCTTTTCCTCTTGGATCCTTCTGAAGAGAAACCATCATGGAGAATTTTGAACGTTCCCGGGCAACCACCTAAATTTGCTTGGGGTCACAGCACCTGCGTGGTCGGAGGGACAAGGGTCCTGGTACTGGGTGGCCACACAGGTGAGGAGTGGATACTCAATGACTTGCATGAGTTGTGCTTAGCAAGCAGGCAGGATTCAGACCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0007623 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009637 GO:0009889 GO:0009909 GO:0009911 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048511 GO:0048518 GO:0048580 GO:0048582 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051239 GO:0051240 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04712 map04712

Pfam Domains

Protein Families

F-box Kelch_2 Kelch_4 PAS_9

Protein Analysis

631

Amino Acids

70.36

Weight (kDa)

5.28

Isoelectric Point (pI)

47.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAS_9	PF13426	68 - 169	7.5e-16	PAS domain
PAS_3	PF08447	74 - 168	8.6e-07	PAS fold
PAS	PF00989	74 - 168	1.4e-06	PAS fold
Kelch_KLHDC2_KLHL20_DRC7	PF24681	293 - 487	1.8e-40	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42	PF13415	298 - 453	4e-26	FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1	PF24981	299 - 435	2.7e-10	Attractin/LZTR1 beta-propeller
Beta-prop_TYW4	PF13418	311 - 554	2.1e-12	tRNA wybutosine-synthesizing protein 4, beta-propeller
Beta-prop_ATRN-LZTR1	PF24981	326 - 516	2.4e-17	Attractin/LZTR1 beta-propeller
Kelch_HCF	PF13854	350 - 608	6.8e-18	Host cell factor, Kelch-repeats domain
Kelch_KLHDC2_KLHL20_DRC7	PF24681	402 - 520	3.8e-17	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_KLHDC2_KLHL20_DRC7	PF24681	481 - 626	2.9e-15	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42	PF13415	522 - 625	5.6e-12	FBX42, beta-propeller domain
Kelch_2	PF07646	536 - 583	1.7e-07	Kelch motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0015839)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68050
fragaria_vesca	FvH4_4g33490
malus_domestica	MD16G1018000.v1.1
prunus_persica	Prupe.1G334000_v2.0.a1
pyrus_communis	pycom16g01630
rosa_chinensis	RchiOBHm_Chr4g0442801
rosa_laevigata	RLG00000005983
rosa_multiflora	Rmu_ssc0000204.1_g000019
rosa_roxburghii	Rroxscaffold_5G00383580
rosa_rugosa	Rorug04G0343300
rosa_samantha	Rh4AG395800 Rh4BG408700 Rh4CG424300 Rh4DG402800
rosa_wichuraiana	Rw4G034210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	558
AarI	CACCTGC	1 cut(s)	1790
Acc36I	ACCTGC	1 cut(s)	1790
AccII	CGCG	1 cut(s)	697
AciI	CCGC	4 cut(s)	1153, 1483, 1586, 1635
AclI	AACGTT	1 cut(s)	1740
AclWI	GGATC	5 cut(s)	338, 1242, 1353, 1697, 1710
AcoI	YGGCCR	3 cut(s)	3, 1432, 1819
AcsI	RAATTY	4 cut(s)	392, 642, 1731, 1760
AcuI	CTGAAG	2 cut(s)	476, 1731
AcyI	GRCGYC	1 cut(s)	856
AfaI	GTAC	4 cut(s)	164, 820, 1320, 1812
AfiI	CCNNNNNNNGG	3 cut(s)	1473, 1585, 1813
AgsI	TTSAA	6 cut(s)	380, 392, 506, 1117, 1662, 1738
AjnI	CCWGG	3 cut(s)	1084, 1250, 1806
AleI	CACNNNNGTG	2 cut(s)	1784, 1827
AluBI	AGCT	6 cut(s)	266, 406, 661, 754, 1260, 1690
AluI	AGCT	6 cut(s)	266, 406, 661, 754, 1260, 1690
Alw21I	GWGCWC	2 cut(s)	268, 1593
Alw26I	GTCTC	8 cut(s)	58, 90, 196, 239, 303, 557, 1398, 1493
AlwI	GGATC	5 cut(s)	338, 1242, 1353, 1697, 1710
AlwNI	CAGNNNCTG	1 cut(s)	1782
Ama87I	CYCGRG	1 cut(s)	1745
AoxI	GGCC	4 cut(s)	3, 1096, 1432, 1819
ApeKI	GCWGC	6 cut(s)	75, 658, 1003, 1040, 1201, 1672
ApoI	RAATTY	4 cut(s)	392, 642, 1731, 1760
AspLEI	GCGC	1 cut(s)	699
AspS9I	GGNCC	2 cut(s)	1096, 1804
AsuC2I	CCSGG	3 cut(s)	1669, 1746, 1747
AsuHPI	GGTGA	9 cut(s)	244, 413, 451, 598, 998, 1071, 1342, 1508, 1841
AvaI	CYCGRG	1 cut(s)	1745
AvaII	GGWCC	1 cut(s)	1804
BaeGI	GKGCMC	1 cut(s)	1479
BalI	TGGCCA	2 cut(s)	5, 1821
BamHI	GGATCC	1 cut(s)	1702
BanII	GRGCYC	1 cut(s)	268
BbsI	GAAGAC	1 cut(s)	1527
Bbv12I	GWGCWC	2 cut(s)	268, 1593
BbvI	GCAGC	6 cut(s)	87, 670, 1015, 1027, 1213, 1659
BccI	CCATC	3 cut(s)	419, 775, 1729
BciT130I	CCWGG	3 cut(s)	1086, 1252, 1808
BciVI	GTATCC	1 cut(s)	1833
BclI	TGATCA	1 cut(s)	1609
BcnI	CCSGG	3 cut(s)	1669, 1746, 1747
BcoDI	GTCTC	8 cut(s)	58, 90, 196, 239, 303, 557, 1398, 1493
BfaI	CTAG	6 cut(s)	864, 1059, 1130, 1245, 1404, 1602
BfmI	CTRYAG	2 cut(s)	656, 736
BfuAI	ACCTGC	1 cut(s)	1790
BfuI	GTATCC	1 cut(s)	1833
BglII	AGATCT	1 cut(s)	529
BisI	GCNGC	7 cut(s)	76, 659, 1004, 1041, 1154, 1202, 1673
BlpI	GCTNAGC	2 cut(s)	155, 1867
BlsI	GCNGC	7 cut(s)	77, 660, 1005, 1042, 1155, 1203, 1674
Bme1390I	CCNGG	6 cut(s)	1086, 1252, 1669, 1746, 1747, 1808
Bme18I	GGWCC	1 cut(s)	1804
BmeT110I	CYCGRG	1 cut(s)	1745
BmgT120I	GGNCC	2 cut(s)	1096, 1804
BmiI	GGNNCC	6 cut(s)	207, 919, 927, 1097, 1704, 1805
BmrFI	CCNGG	6 cut(s)	1086, 1252, 1669, 1746, 1747, 1808
BmrI	ACTGGG	1 cut(s)	1823
BmsI	GCATC	7 cut(s)	141, 187, 728, 1027, 1183, 1292, 1300
BmuI	ACTGGG	1 cut(s)	1823
BoxI	GACNNNNGTC	1 cut(s)	1802
BpiI	GAAGAC	1 cut(s)	1527
BpmI	CTGGAG	1 cut(s)	1568
Bpu10I	CCTNAGC	1 cut(s)	449
Bpu1102I	GCTNAGC	2 cut(s)	155, 1867
BpuEI	CTTGAG	1 cut(s)	902
BpuMI	CCSGG	3 cut(s)	1669, 1746, 1747
BsaAI	YACGTR	1 cut(s)	1339
BsaHI	GRCGYC	1 cut(s)	856
BsaI	GGTCTC	3 cut(s)	196, 303, 1398
BsaJI	CCNNGG	6 cut(s)	6, 277, 435, 707, 921, 1745
BsaWI	WCCGGW	1 cut(s)	254
BsaXI	ACNNNNNCTCC	2 cut(s)	906, 936
Bsc4I	CCNNNNNNNGG	3 cut(s)	1473, 1585, 1813
Bse118I	RCCGGY	1 cut(s)	1434
Bse1I	ACTGG	6 cut(s)	551, 609, 820, 975, 1551, 1818
BseBI	CCWGG	3 cut(s)	1086, 1252, 1808
BseDI	CCNNGG	6 cut(s)	6, 277, 435, 707, 921, 1745
BseLI	CCNNNNNNNGG	3 cut(s)	1473, 1585, 1813
BseMII	CTCAG	4 cut(s)	351, 564, 599, 1700
BseNI	ACTGG	6 cut(s)	551, 609, 820, 975, 1551, 1818
BseSI	GKGCMC	1 cut(s)	1479
BseXI	GCAGC	6 cut(s)	87, 670, 1015, 1027, 1213, 1659
BseYI	CCCAGC	1 cut(s)	1313
Bsh1236I	CGCG	1 cut(s)	697
Bsh1285I	CGRYCG	1 cut(s)	283
BshFI	GGCC	4 cut(s)	5, 1098, 1434, 1821
BsiEI	CGRYCG	1 cut(s)	283
BsiHKAI	GWGCWC	2 cut(s)	268, 1593
BsiHKCI	CYCGRG	1 cut(s)	1745
BsiSI	CCGG	4 cut(s)	255, 1435, 1668, 1746
BslFI	GGGAC	2 cut(s)	1425, 1810
BslI	CCNNNNNNNGG	3 cut(s)	1473, 1585, 1813
BsmAI	GTCTC	8 cut(s)	58, 90, 196, 239, 303, 557, 1398, 1493
BsmBI	CGTCTC	2 cut(s)	90, 239
BsmFI	GGGAC	2 cut(s)	1425, 1810
BsmI	GAATGC	2 cut(s)	501, 1675
BsnI	GGCC	4 cut(s)	5, 1098, 1434, 1821
Bso31I	GGTCTC	3 cut(s)	196, 303, 1398
BsoBI	CYCGRG	1 cut(s)	1745
Bsp1286I	GDGCHC	3 cut(s)	268, 1479, 1593
Bsp143I	GATC	8 cut(s)	219, 343, 529, 1247, 1345, 1490, 1609, 1702
Bsp1720I	GCTNAGC	2 cut(s)	155, 1867
Bsp19I	CCATGG	1 cut(s)	6
BspACI	CCGC	4 cut(s)	1153, 1483, 1586, 1635
BspANI	GGCC	4 cut(s)	5, 1098, 1434, 1821
BspCNI	CTCAG	4 cut(s)	352, 565, 598, 1699
BspFNI	CGCG	1 cut(s)	697
BspHI	TCATGA	1 cut(s)	268
BspLI	GGNNCC	6 cut(s)	207, 919, 927, 1097, 1704, 1805
BspMAI	CTGCAG	2 cut(s)	660, 740
BspMI	ACCTGC	1 cut(s)	1790
BspPI	GGATC	5 cut(s)	338, 1242, 1353, 1697, 1710
BspQI	GCTCTTC	1 cut(s)	1521
BspTNI	GGTCTC	3 cut(s)	196, 303, 1398
BsrFI	RCCGGY	1 cut(s)	1434
BsrI	ACTGG	6 cut(s)	551, 609, 820, 975, 1551, 1818
BssAI	RCCGGY	1 cut(s)	1434
BssECI	CCNNGG	6 cut(s)	6, 277, 435, 707, 921, 1745
BssMI	GATC	8 cut(s)	219, 343, 529, 1247, 1345, 1490, 1609, 1702
BssNI	GRCGYC	1 cut(s)	856
BssT1I	CCWWGG	3 cut(s)	6, 435, 707
Bst2UI	CCWGG	3 cut(s)	1086, 1252, 1808
Bst4CI	ACNGT	5 cut(s)	290, 484, 910, 936, 1472
Bst6I	CTCTTC	7 cut(s)	24, 56, 117, 137, 799, 1521, 1707
BstACI	GRCGYC	1 cut(s)	856
BstAPI	GCANNNNNTGC	2 cut(s)	1162, 1632
BstBAI	YACGTR	1 cut(s)	1339
BstC8I	GCNNGC	3 cut(s)	1628, 1873, 1877
BstDSI	CCRYGG	2 cut(s)	6, 921
BstFNI	CGCG	1 cut(s)	697
BstHHI	GCGC	1 cut(s)	699
BstKTI	GATC	8 cut(s)	222, 346, 532, 1250, 1348, 1493, 1612, 1705
BstMAI	GTCTC	8 cut(s)	58, 90, 196, 239, 303, 557, 1398, 1493
BstMBI	GATC	8 cut(s)	219, 343, 529, 1247, 1345, 1490, 1609, 1702
BstMCI	CGRYCG	1 cut(s)	283
BstMWI	GCNNNNNNNGC	4 cut(s)	1162, 1575, 1632, 1872
BstNI	CCWGG	3 cut(s)	1086, 1252, 1808
BstNSI	RCATGY	4 cut(s)	163, 570, 773, 1630
BstPAI	GACNNNNGTC	1 cut(s)	1802
BstSCI	CCNGG	6 cut(s)	1084, 1250, 1667, 1744, 1745, 1806
BstSFI	CTRYAG	2 cut(s)	656, 736
BstSLI	GKGCMC	1 cut(s)	1479
BstSNI	TACGTA	1 cut(s)	1339
BstUI	CGCG	1 cut(s)	697
BstV1I	GCAGC	6 cut(s)	87, 670, 1015, 1027, 1213, 1659
BstV2I	GAAGAC	1 cut(s)	1527
BstX2I	RGATCY	5 cut(s)	343, 529, 1247, 1345, 1702
BstXI	CCANNNNNNTGG	3 cut(s)	1092, 1389, 1516
BstYI	RGATCY	5 cut(s)	343, 529, 1247, 1345, 1702
BsuI	GTATCC	1 cut(s)	1833
BsuRI	GGCC	4 cut(s)	5, 1098, 1434, 1821
BtgI	CCRYGG	2 cut(s)	6, 921
BtsIMutI	CAGTG	6 cut(s)	570, 616, 813, 952, 1353, 1477
BveI	ACCTGC	1 cut(s)	1790
Cac8I	GCNNGC	3 cut(s)	1628, 1873, 1877
CaiI	CAGNNNCTG	1 cut(s)	1782
CciI	TCATGA	1 cut(s)	268
CfoI	GCGC	1 cut(s)	699
Cfr10I	RCCGGY	1 cut(s)	1434
Cfr13I	GGNCC	2 cut(s)	1096, 1804
Cfr9I	CCCGGG	1 cut(s)	1745
CseI	GACGC	1 cut(s)	845
CsiI	ACCWGGT	1 cut(s)	1084
Csp6I	GTAC	4 cut(s)	163, 819, 1319, 1811
CspCI	CAANNNNNGTGG	4 cut(s)	166, 201, 696, 731
CviQI	GTAC	4 cut(s)	163, 819, 1319, 1811
DpnI	GATC	8 cut(s)	221, 345, 531, 1249, 1347, 1492, 1611, 1704
DpnII	GATC	8 cut(s)	219, 343, 529, 1247, 1345, 1490, 1609, 1702
EaeI	YGGCCR	3 cut(s)	3, 1432, 1819
Eam1104I	CTCTTC	7 cut(s)	24, 56, 117, 137, 799, 1521, 1707
EarI	CTCTTC	7 cut(s)	24, 56, 117, 137, 799, 1521, 1707
Ecl136II	GAGCTC	1 cut(s)	266
Eco105I	TACGTA	1 cut(s)	1339
Eco130I	CCWWGG	3 cut(s)	6, 435, 707
Eco24I	GRGCYC	1 cut(s)	268
Eco31I	GGTCTC	3 cut(s)	196, 303, 1398
Eco47I	GGWCC	1 cut(s)	1804
Eco53kI	GAGCTC	1 cut(s)	266
Eco57I	CTGAAG	2 cut(s)	476, 1731
Eco88I	CYCGRG	1 cut(s)	1745
EcoICRI	GAGCTC	1 cut(s)	266
EcoO109I	RGGNCCY	1 cut(s)	1804
EcoRI	GAATTC	1 cut(s)	392
EcoRII	CCWGG	3 cut(s)	1084, 1250, 1806
EcoT14I	CCWWGG	3 cut(s)	6, 435, 707
EcoT22I	ATGCAT	2 cut(s)	799, 1561
EcoT38I	GRGCYC	1 cut(s)	268
ErhI	CCWWGG	3 cut(s)	6, 435, 707
Esp3I	CGTCTC	2 cut(s)	90, 239
FaqI	GGGAC	2 cut(s)	1425, 1810
FbaI	TGATCA	1 cut(s)	1609
Fnu4HI	GCNGC	7 cut(s)	76, 659, 1004, 1041, 1154, 1202, 1673
FriOI	GRGCYC	1 cut(s)	268
Fsp4HI	GCNGC	7 cut(s)	76, 659, 1004, 1041, 1154, 1202, 1673
FspBI	CTAG	6 cut(s)	864, 1059, 1130, 1245, 1404, 1602
GlaI	GCGC	1 cut(s)	698
GluI	GCNGC	7 cut(s)	76, 659, 1004, 1041, 1154, 1202, 1673
GsaI	CCCAGC	1 cut(s)	1317
GsuI	CTGGAG	1 cut(s)	1568
HaeIII	GGCC	4 cut(s)	5, 1098, 1434, 1821
HapII	CCGG	4 cut(s)	255, 1435, 1668, 1746
HgaI	GACGC	1 cut(s)	845
HhaI	GCGC	1 cut(s)	699
Hin1I	GRCGYC	1 cut(s)	856
Hin6I	GCGC	1 cut(s)	697
HinP1I	GCGC	1 cut(s)	697
HincII	GTYRAC	1 cut(s)	232
HindII	GTYRAC	1 cut(s)	232
HinfI	GANTC	7 cut(s)	172, 652, 703, 744, 1378, 1444, 1883
HpaII	CCGG	4 cut(s)	255, 1435, 1668, 1746
HphI	GGTGA	9 cut(s)	244, 413, 451, 598, 998, 1071, 1342, 1508, 1841
Hpy166II	GTNNAC	7 cut(s)	232, 252, 582, 614, 906, 1056, 1460
Hpy8I	GTNNAC	7 cut(s)	232, 252, 582, 614, 906, 1056, 1460
HpyCH4III	ACNGT	5 cut(s)	290, 484, 910, 936, 1472
HpyCH4IV	ACGT	4 cut(s)	228, 248, 1338, 1740
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1162, 1575, 1632, 1872
HpySE526I	ACGT	4 cut(s)	228, 248, 1338, 1740
Hsp92I	GRCGYC	1 cut(s)	856
HspAI	GCGC	1 cut(s)	697
Ksp22I	TGATCA	1 cut(s)	1609
Kzo9I	GATC	8 cut(s)	219, 343, 529, 1247, 1345, 1490, 1609, 1702
LguI	GCTCTTC	1 cut(s)	1521
LmnI	GCTCC	3 cut(s)	263, 925, 1596
Lsp1109I	GCAGC	6 cut(s)	87, 670, 1015, 1027, 1213, 1659
LweI	GCATC	7 cut(s)	141, 187, 728, 1027, 1183, 1292, 1300
MabI	ACCWGGT	1 cut(s)	1084
MaeI	CTAG	6 cut(s)	864, 1059, 1130, 1245, 1404, 1602
MaeII	ACGT	4 cut(s)	228, 248, 1338, 1740
MaeIII	GTNAC	3 cut(s)	484, 811, 1772
MalI	GATC	8 cut(s)	221, 345, 531, 1249, 1347, 1492, 1611, 1704
MboI	GATC	8 cut(s)	219, 343, 529, 1247, 1345, 1490, 1609, 1702
MfeI	CAATTG	1 cut(s)	1662
MflI	RGATCY	5 cut(s)	343, 529, 1247, 1345, 1702
MhlI	GDGCHC	3 cut(s)	268, 1479, 1593
MlsI	TGGCCA	2 cut(s)	5, 1821
MluCI	AATT	8 cut(s)	392, 412, 642, 940, 1283, 1662, 1731, 1760
MluNI	TGGCCA	2 cut(s)	5, 1821
MlyI	GAGTC	2 cut(s)	166, 697
MmeI	TCCRAC	6 cut(s)	263, 875, 892, 1388, 1406, 1771
Mox20I	TGGCCA	2 cut(s)	5, 1821
Mph1103I	ATGCAT	2 cut(s)	799, 1561
MscI	TGGCCA	2 cut(s)	5, 1821
MseI	TTAA	2 cut(s)	411, 1487
MslI	CAYNNNNRTG	3 cut(s)	1108, 1784, 1827
Msp20I	TGGCCA	2 cut(s)	5, 1821
MspI	CCGG	4 cut(s)	255, 1435, 1668, 1746
MspR9I	CCNGG	6 cut(s)	1086, 1252, 1669, 1746, 1747, 1808
MunI	CAATTG	1 cut(s)	1662
Mva1269I	GAATGC	2 cut(s)	501, 1675
MvaI	CCWGG	3 cut(s)	1086, 1252, 1808
MvnI	CGCG	1 cut(s)	697
MwoI	GCNNNNNNNGC	4 cut(s)	1162, 1575, 1632, 1872
NciI	CCSGG	3 cut(s)	1669, 1746, 1747
NcoI	CCATGG	1 cut(s)	6
NdeII	GATC	8 cut(s)	219, 343, 529, 1247, 1345, 1490, 1609, 1702
NlaIV	GGNNCC	6 cut(s)	207, 919, 927, 1097, 1704, 1805
NmuCI	GTSAC	3 cut(s)	484, 811, 1772
NsiI	ATGCAT	2 cut(s)	799, 1561
NspI	RCATGY	4 cut(s)	163, 570, 773, 1630
OliI	CACNNNNGTG	2 cut(s)	1784, 1827
PaeI	GCATGC	1 cut(s)	1630
PagI	TCATGA	1 cut(s)	268
PaqCI	CACCTGC	1 cut(s)	1790
PciSI	GCTCTTC	1 cut(s)	1521
PctI	GAATGC	2 cut(s)	501, 1675
PfeI	GAWTC	5 cut(s)	652, 744, 1378, 1444, 1883
PkrI	GCNGC	7 cut(s)	77, 660, 1005, 1042, 1155, 1203, 1674
PleI	GAGTC	2 cut(s)	166, 697
PpsI	GAGTC	2 cut(s)	166, 697
Ppu21I	YACGTR	1 cut(s)	1339
PpuMI	RGGWCCY	1 cut(s)	1804
PshAI	GACNNNNGTC	1 cut(s)	1802
PsiI	TTATAA	1 cut(s)	558
Psp124BI	GAGCTC	1 cut(s)	268
Psp1406I	AACGTT	1 cut(s)	1740
Psp5II	RGGWCCY	1 cut(s)	1804
Psp6I	CCWGG	3 cut(s)	1084, 1250, 1806
PspFI	CCCAGC	1 cut(s)	1313
PspGI	CCWGG	3 cut(s)	1084, 1250, 1806
PspN4I	GGNNCC	6 cut(s)	207, 919, 927, 1097, 1704, 1805
PspPI	GGNCC	2 cut(s)	1096, 1804
PspPPI	RGGWCCY	1 cut(s)	1804
PstI	CTGCAG	2 cut(s)	660, 740
PstNI	CAGNNNCTG	1 cut(s)	1782
PsuI	RGATCY	5 cut(s)	343, 529, 1247, 1345, 1702
RsaI	GTAC	4 cut(s)	164, 820, 1320, 1812
RsaNI	GTAC	4 cut(s)	163, 819, 1319, 1811
RseI	CAYNNNNRTG	3 cut(s)	1108, 1784, 1827
SacI	GAGCTC	1 cut(s)	268
SapI	GCTCTTC	1 cut(s)	1521
SaqAI	TTAA	2 cut(s)	411, 1487
SatI	GCNGC	7 cut(s)	76, 659, 1004, 1041, 1154, 1202, 1673
Sau3AI	GATC	8 cut(s)	219, 343, 529, 1247, 1345, 1490, 1609, 1702
Sau96I	GGNCC	2 cut(s)	1096, 1804
SchI	GAGTC	2 cut(s)	166, 697
ScrFI	CCNGG	6 cut(s)	1086, 1252, 1669, 1746, 1747, 1808
SduI	GDGCHC	3 cut(s)	268, 1479, 1593
SexAI	ACCWGGT	1 cut(s)	1084
SfaNI	GCATC	7 cut(s)	141, 187, 728, 1027, 1183, 1292, 1300
SfcI	CTRYAG	2 cut(s)	656, 736
SinI	GGWCC	1 cut(s)	1804
SmaI	CCCGGG	1 cut(s)	1747
SmiMI	CAYNNNNRTG	3 cut(s)	1108, 1784, 1827
SmlI	CTYRAG	1 cut(s)	881
SmoI	CTYRAG	1 cut(s)	881
SnaBI	TACGTA	1 cut(s)	1339
SphI	GCATGC	1 cut(s)	1630
Sse9I	AATT	8 cut(s)	392, 412, 642, 940, 1283, 1662, 1731, 1760
SsiI	CCGC	4 cut(s)	1153, 1483, 1586, 1635
SspI	AATATT	1 cut(s)	599
SspMI	CTAG	6 cut(s)	864, 1059, 1130, 1245, 1404, 1602
SstI	GAGCTC	1 cut(s)	268
StyD4I	CCNGG	6 cut(s)	1084, 1250, 1667, 1744, 1745, 1806
StyI	CCWWGG	3 cut(s)	6, 435, 707
TaaI	ACNGT	5 cut(s)	290, 484, 910, 936, 1472
TaiI	ACGT	4 cut(s)	231, 251, 1341, 1743
TaqI	TCGA	3 cut(s)	94, 243, 967
TaqII	GACCGA	1 cut(s)	269
TasI	AATT	8 cut(s)	392, 412, 642, 940, 1283, 1662, 1731, 1760
TauI	GCSGC	1 cut(s)	1156
TfiI	GAWTC	5 cut(s)	652, 744, 1378, 1444, 1883
Tru1I	TTAA	2 cut(s)	411, 1487
Tru9I	TTAA	2 cut(s)	411, 1487
TscAI	CASTG	6 cut(s)	577, 616, 820, 952, 1360, 1477
TseFI	GTSAC	3 cut(s)	484, 811, 1772
TseI	GCWGC	6 cut(s)	75, 658, 1003, 1040, 1201, 1672
Tsp45I	GTSAC	3 cut(s)	484, 811, 1772
TspDTI	ATGAA	5 cut(s)	120, 285, 684, 1098, 1377
TspGWI	ACGGA	4 cut(s)	492, 737, 910, 1322
TspMI	CCCGGG	1 cut(s)	1745
TspRI	CASTG	6 cut(s)	577, 616, 820, 952, 1360, 1477
VpaK11BI	GGWCC	1 cut(s)	1804
XapI	RAATTY	4 cut(s)	392, 642, 1731, 1760
XceI	RCATGY	4 cut(s)	163, 570, 773, 1630
XcmI	CCANNNNNNNNNTGG	2 cut(s)	184, 1089
XmaI	CCCGGG	1 cut(s)	1745
XspI	CTAG	6 cut(s)	864, 1059, 1130, 1245, 1404, 1602
Zsp2I	ATGCAT	2 cut(s)	799, 1561

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.