Rroxscaffold_5G00383580

Adagio protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0015839
Physical Location & Seq
Forward (+)
63188690 .. 63191729
3040 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00383580.1

Sequence Viewer

Length: 762 bp
ATGCAGCCAATGGATGACACATTTGTTCTCAATCTTGATGCTGCCAATCCAGAGTGGACTAGAGTAAGTGTGAAATCATCACCACCAGGTCGGTGGGGCCATACACTTTCATGTTTGAATGGTTCTTTGCTAGTAGTTTTTGGGGGATGTGGGCGGCAAGGGTTGCTCAATGATGTTTGGGTTCTTGACTTGGATGCCAAGCAGCCAACATGGAAAGAAGTTTATGGTGGAACTCCTCCTCTCCCTAGATCCTGGCATAGCTCTTGCATAACAGAAGGTTCTAAATTAGTTGTGTCAGGTGGATGCACGGATGCTGGGGTACTTCTTAGCGACACATACGTATTGGATCTCACCACTGACAATCCAACATGGAGAGAGATTCCAACTTCATGGTCTCCTCCCTCTAGGTTGGGACATTCCCTTTCAGTTTATGGCCGGTCAAAGATTCTCATGTTTGGTGGACTTGCCAACAGTGGGCACTTGCGGTTAAGATCAGGTGAGACTTACACCATTGATTTGGAAGACGAAGAGCCTCAATGGAGGCAACTGGAGTGTAATGCATTTACCAGCATTGACAGCCAGAGTGCAGTGCTCCCTCCTCCTAGACTTGATCATGTTGCTGTGAGCATGCCTTGTGGAAGGATTATCATTTTTGGTGGTTCAATTGCCGGGCTGCATTCTCCTTCTCAGCTTTTCCTCTTGGATCCTTCTGAAGAGAAACCATCGTGGAGAATTTTGAACGTTCCCGGGGCAACCACCTAA

Protein Analysis

253

Amino Acids

27.55

Weight (kDa)

5.32

Isoelectric Point (pI)

54.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_TYW4 PF13418 2 - 221 1.1e-10 tRNA wybutosine-synthesizing protein 4, beta-propeller
Kelch_KLHDC2_KLHL20_DRC7 PF24681 4 - 154 3.6e-30 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 4 - 120 1e-21 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 7 - 221 6.7e-19 Attractin/LZTR1 beta-propeller
Kelch_HCF PF13854 17 - 221 5.1e-16 Host cell factor, Kelch-repeats domain
Beta-prop_FBX42 PF13415 121 - 236 6.1e-10 FBX42, beta-propeller domain
Kelch_2 PF07646 203 - 250 4.5e-08 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015839)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68050
fragaria_vesca FvH4_4g33490
malus_domestica MD16G1018000.v1.1
prunus_persica Prupe.1G334000_v2.0.a1
pyrus_communis pycom16g01630
rosa_chinensis RchiOBHm_Chr4g0442801
rosa_laevigata RLG00000005983
rosa_multiflora Rmu_ssc0000204.1_g000019
rosa_roxburghii Rroxscaffold_5G00383580
rosa_rugosa Rorug04G0343300
rosa_samantha Rh4AG395800 Rh4BG408700 Rh4CG424300 Rh4DG402800
rosa_wichuraiana Rw4G034210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 154, 484
AclI AACGTT 1 cut(s) 741
AclWI GGATC 4 cut(s) 243, 354, 698, 711
AcoI YGGCCR 1 cut(s) 433
AcsI RAATTY 1 cut(s) 732
AcuI CTGAAG 1 cut(s) 732
AfaI GTAC 1 cut(s) 321
AfiI CCNNNNNNNGG 1 cut(s) 474
AgsI TTSAA 3 cut(s) 118, 663, 739
AjnI CCWGG 2 cut(s) 85, 251
AluBI AGCT 2 cut(s) 261, 691
AluI AGCT 2 cut(s) 261, 691
Alw21I GWGCWC 1 cut(s) 594
Alw26I GTCTC 2 cut(s) 399, 494
AlwI GGATC 4 cut(s) 243, 354, 698, 711
Ama87I CYCGRG 1 cut(s) 746
AoxI GGCC 2 cut(s) 97, 433
ApeKI GCWGC 4 cut(s) 4, 41, 202, 673
ApoI RAATTY 1 cut(s) 732
AspS9I GGNCC 1 cut(s) 97
AsuC2I CCSGG 3 cut(s) 670, 747, 748
AsuHPI GGTGA 3 cut(s) 72, 343, 509
AvaI CYCGRG 1 cut(s) 746
BaeGI GKGCMC 1 cut(s) 480
BamHI GGATCC 1 cut(s) 703
BbsI GAAGAC 1 cut(s) 528
Bbv12I GWGCWC 1 cut(s) 594
BbvI GCAGC 4 cut(s) 16, 28, 214, 660
BccI CCATC 1 cut(s) 730
BciT130I CCWGG 2 cut(s) 87, 253
BclI TGATCA 1 cut(s) 610
BcnI CCSGG 3 cut(s) 670, 747, 748
BcoDI GTCTC 2 cut(s) 399, 494
BfaI CTAG 5 cut(s) 60, 131, 246, 405, 603
BisI GCNGC 5 cut(s) 5, 42, 155, 203, 674
BlsI GCNGC 5 cut(s) 6, 43, 156, 204, 675
Bme1390I CCNGG 5 cut(s) 87, 253, 670, 747, 748
BmeT110I CYCGRG 1 cut(s) 746
BmgT120I GGNCC 1 cut(s) 97
BmiI GGNNCC 2 cut(s) 98, 705
BmrFI CCNGG 5 cut(s) 87, 253, 670, 747, 748
BmsI GCATC 4 cut(s) 28, 184, 293, 301
BpiI GAAGAC 1 cut(s) 528
BpmI CTGGAG 1 cut(s) 569
BpuMI CCSGG 3 cut(s) 670, 747, 748
BsaAI YACGTR 1 cut(s) 340
BsaI GGTCTC 1 cut(s) 399
BsaJI CCNNGG 2 cut(s) 746, 747
Bsc4I CCNNNNNNNGG 1 cut(s) 474
Bse118I RCCGGY 1 cut(s) 435
Bse1I ACTGG 1 cut(s) 552
BseBI CCWGG 2 cut(s) 87, 253
BseDI CCNNGG 2 cut(s) 746, 747
BseGI GGATG 5 cut(s) 19, 152, 199, 308, 316
BseLI CCNNNNNNNGG 1 cut(s) 474
BseMII CTCAG 1 cut(s) 701
BseNI ACTGG 1 cut(s) 552
BseRI GAGGAG 4 cut(s) 225, 228, 387, 588
BseSI GKGCMC 1 cut(s) 480
BseXI GCAGC 4 cut(s) 16, 28, 214, 660
BseYI CCCAGC 1 cut(s) 314
BsgI GTGCAG 1 cut(s) 606
BshFI GGCC 2 cut(s) 99, 435
BsiHKAI GWGCWC 1 cut(s) 594
BsiHKCI CYCGRG 1 cut(s) 746
BsiSI CCGG 3 cut(s) 436, 669, 747
BslFI GGGAC 1 cut(s) 426
BslI CCNNNNNNNGG 1 cut(s) 474
BsmAI GTCTC 2 cut(s) 399, 494
BsmFI GGGAC 1 cut(s) 426
BsmI GAATGC 1 cut(s) 676
BsnI GGCC 2 cut(s) 99, 435
Bso31I GGTCTC 1 cut(s) 399
BsoBI CYCGRG 1 cut(s) 746
Bsp1286I GDGCHC 2 cut(s) 480, 594
Bsp143I GATC 5 cut(s) 248, 346, 491, 610, 703
BspACI CCGC 2 cut(s) 154, 484
BspANI GGCC 2 cut(s) 99, 435
BspCNI CTCAG 1 cut(s) 700
BspLI GGNNCC 2 cut(s) 98, 705
BspPI GGATC 4 cut(s) 243, 354, 698, 711
BspQI GCTCTTC 1 cut(s) 522
BspTNI GGTCTC 1 cut(s) 399
BsrFI RCCGGY 1 cut(s) 435
BsrI ACTGG 1 cut(s) 552
BssAI RCCGGY 1 cut(s) 435
BssECI CCNNGG 2 cut(s) 746, 747
BssMI GATC 5 cut(s) 248, 346, 491, 610, 703
Bst2UI CCWGG 2 cut(s) 87, 253
Bst4CI ACNGT 1 cut(s) 473
Bst6I CTCTTC 2 cut(s) 522, 708
BstAPI GCANNNNNTGC 1 cut(s) 163
BstBAI YACGTR 1 cut(s) 340
BstC8I GCNNGC 1 cut(s) 629
BstDEI CTNAG 2 cut(s) 326, 687
BstF5I GGATG 5 cut(s) 19, 152, 199, 308, 316
BstKTI GATC 5 cut(s) 251, 349, 494, 613, 706
BstMAI GTCTC 2 cut(s) 399, 494
BstMBI GATC 5 cut(s) 248, 346, 491, 610, 703
BstMWI GCNNNNNNNGC 2 cut(s) 163, 576
BstNI CCWGG 2 cut(s) 87, 253
BstNSI RCATGY 1 cut(s) 631
BstSCI CCNGG 5 cut(s) 85, 251, 668, 745, 746
BstSLI GKGCMC 1 cut(s) 480
BstSNI TACGTA 1 cut(s) 340
BstV1I GCAGC 4 cut(s) 16, 28, 214, 660
BstV2I GAAGAC 1 cut(s) 528
BstX2I RGATCY 3 cut(s) 248, 346, 703
BstXI CCANNNNNNTGG 3 cut(s) 93, 390, 517
BstYI RGATCY 3 cut(s) 248, 346, 703
BsuRI GGCC 2 cut(s) 99, 435
BtsCI GGATG 5 cut(s) 19, 152, 199, 308, 316
BtsI GCAGTG 1 cut(s) 594
BtsIMutI CAGTG 3 cut(s) 354, 478, 594
Cac8I GCNNGC 1 cut(s) 629
Cfr10I RCCGGY 1 cut(s) 435
Cfr13I GGNCC 1 cut(s) 97
Cfr9I CCCGGG 1 cut(s) 746
CsiI ACCWGGT 1 cut(s) 85
Csp6I GTAC 1 cut(s) 320
CviAII CATG 7 cut(s) 111, 210, 369, 390, 451, 614, 628
CviJI RGCY 9 cut(s) 7, 99, 205, 261, 435, 532, 579, 673, 691
CviKI_1 RGCY 9 cut(s) 7, 99, 205, 261, 435, 532, 579, 673, 691
CviQI GTAC 1 cut(s) 320
DdeI CTNAG 2 cut(s) 326, 687
DpnI GATC 5 cut(s) 250, 348, 493, 612, 705
DpnII GATC 5 cut(s) 248, 346, 491, 610, 703
EaeI YGGCCR 1 cut(s) 433
Eam1104I CTCTTC 2 cut(s) 522, 708
EarI CTCTTC 2 cut(s) 522, 708
Eco105I TACGTA 1 cut(s) 340
Eco31I GGTCTC 1 cut(s) 399
Eco57I CTGAAG 1 cut(s) 732
Eco88I CYCGRG 1 cut(s) 746
EcoRII CCWGG 2 cut(s) 85, 251
EcoT22I ATGCAT 1 cut(s) 562
FaeI CATG 7 cut(s) 114, 213, 372, 393, 454, 617, 631
FaqI GGGAC 1 cut(s) 426
FatI CATG 7 cut(s) 110, 209, 368, 389, 450, 613, 627
FbaI TGATCA 1 cut(s) 610
Fnu4HI GCNGC 5 cut(s) 5, 42, 155, 203, 674
FokI GGATG 5 cut(s) 26, 159, 206, 315, 323
Fsp4HI GCNGC 5 cut(s) 5, 42, 155, 203, 674
FspBI CTAG 5 cut(s) 60, 131, 246, 405, 603
GluI GCNGC 5 cut(s) 5, 42, 155, 203, 674
GsaI CCCAGC 1 cut(s) 318
GsuI CTGGAG 1 cut(s) 569
HaeIII GGCC 2 cut(s) 99, 435
HapII CCGG 3 cut(s) 436, 669, 747
Hin1II CATG 7 cut(s) 114, 213, 372, 393, 454, 617, 631
HinfI GANTC 2 cut(s) 379, 445
HpaII CCGG 3 cut(s) 436, 669, 747
HphI GGTGA 3 cut(s) 72, 343, 509
Hpy166II GTNNAC 2 cut(s) 57, 461
Hpy188I TCNGA 1 cut(s) 712
Hpy188III TCNNGA 3 cut(s) 35, 50, 185
Hpy8I GTNNAC 2 cut(s) 57, 461
HpyAV CCTTC 4 cut(s) 269, 633, 693, 717
HpyCH4III ACNGT 1 cut(s) 473
HpyCH4IV ACGT 2 cut(s) 339, 741
HpyCH4V TGCA 6 cut(s) 4, 267, 306, 560, 587, 676
HpyF10VI GCNNNNNNNGC 2 cut(s) 163, 576
HpyF3I CTNAG 2 cut(s) 326, 687
HpySE526I ACGT 2 cut(s) 339, 741
Hsp92II CATG 7 cut(s) 114, 213, 372, 393, 454, 617, 631
Ksp22I TGATCA 1 cut(s) 610
Kzo9I GATC 5 cut(s) 248, 346, 491, 610, 703
LguI GCTCTTC 1 cut(s) 522
LmnI GCTCC 1 cut(s) 597
Lsp1109I GCAGC 4 cut(s) 16, 28, 214, 660
LweI GCATC 4 cut(s) 28, 184, 293, 301
MabI ACCWGGT 1 cut(s) 85
MaeI CTAG 5 cut(s) 60, 131, 246, 405, 603
MaeII ACGT 2 cut(s) 339, 741
MalI GATC 5 cut(s) 250, 348, 493, 612, 705
MboI GATC 5 cut(s) 248, 346, 491, 610, 703
MboII GAAGA 3 cut(s) 533, 539, 725
MfeI CAATTG 1 cut(s) 663
MflI RGATCY 3 cut(s) 248, 346, 703
MhlI GDGCHC 2 cut(s) 480, 594
MluCI AATT 3 cut(s) 284, 663, 732
MmeI TCCRAC 2 cut(s) 389, 407
MnlI CCTC 9 cut(s) 246, 249, 408, 412, 534, 543, 606, 609, 707
Mph1103I ATGCAT 1 cut(s) 562
MseI TTAA 1 cut(s) 488
MslI CAYNNNNRTG 1 cut(s) 109
MspI CCGG 3 cut(s) 436, 669, 747
MspR9I CCNGG 5 cut(s) 87, 253, 670, 747, 748
MunI CAATTG 1 cut(s) 663
Mva1269I GAATGC 1 cut(s) 676
MvaI CCWGG 2 cut(s) 87, 253
MwoI GCNNNNNNNGC 2 cut(s) 163, 576
NciI CCSGG 3 cut(s) 670, 747, 748
NdeII GATC 5 cut(s) 248, 346, 491, 610, 703
NlaIII CATG 7 cut(s) 114, 213, 372, 393, 454, 617, 631
NlaIV GGNNCC 2 cut(s) 98, 705
NsiI ATGCAT 1 cut(s) 562
NspI RCATGY 1 cut(s) 631
PaeI GCATGC 1 cut(s) 631
PciSI GCTCTTC 1 cut(s) 522
PctI GAATGC 1 cut(s) 676
PfeI GAWTC 2 cut(s) 379, 445
PkrI GCNGC 5 cut(s) 6, 43, 156, 204, 675
Ppu21I YACGTR 1 cut(s) 340
Psp1406I AACGTT 1 cut(s) 741
Psp6I CCWGG 2 cut(s) 85, 251
PspFI CCCAGC 1 cut(s) 314
PspGI CCWGG 2 cut(s) 85, 251
PspN4I GGNNCC 2 cut(s) 98, 705
PspPI GGNCC 1 cut(s) 97
PsuI RGATCY 3 cut(s) 248, 346, 703
RsaI GTAC 1 cut(s) 321
RsaNI GTAC 1 cut(s) 320
RseI CAYNNNNRTG 1 cut(s) 109
SapI GCTCTTC 1 cut(s) 522
SaqAI TTAA 1 cut(s) 488
SatI GCNGC 5 cut(s) 5, 42, 155, 203, 674
Sau3AI GATC 5 cut(s) 248, 346, 491, 610, 703
Sau96I GGNCC 1 cut(s) 97
ScrFI CCNGG 5 cut(s) 87, 253, 670, 747, 748
SduI GDGCHC 2 cut(s) 480, 594
SexAI ACCWGGT 1 cut(s) 85
SfaNI GCATC 4 cut(s) 28, 184, 293, 301
SmaI CCCGGG 1 cut(s) 748
SmiMI CAYNNNNRTG 1 cut(s) 109
SnaBI TACGTA 1 cut(s) 340
SphI GCATGC 1 cut(s) 631
Sse9I AATT 3 cut(s) 284, 663, 732
SsiI CCGC 2 cut(s) 154, 484
SspMI CTAG 5 cut(s) 60, 131, 246, 405, 603
StyD4I CCNGG 5 cut(s) 85, 251, 668, 745, 746
TaaI ACNGT 1 cut(s) 473
TaiI ACGT 2 cut(s) 342, 744
TasI AATT 3 cut(s) 284, 663, 732
TauI GCSGC 1 cut(s) 157
TfiI GAWTC 2 cut(s) 379, 445
Tru1I TTAA 1 cut(s) 488
Tru9I TTAA 1 cut(s) 488
TscAI CASTG 3 cut(s) 361, 478, 594
TseI GCWGC 4 cut(s) 4, 41, 202, 673
TspDTI ATGAA 2 cut(s) 99, 378
TspGWI ACGGA 1 cut(s) 323
TspMI CCCGGG 1 cut(s) 746
TspRI CASTG 3 cut(s) 361, 478, 594
XapI RAATTY 1 cut(s) 732
XceI RCATGY 1 cut(s) 631
XcmI CCANNNNNNNNNTGG 1 cut(s) 90
XmaI CCCGGG 1 cut(s) 746
XspI CTAG 5 cut(s) 60, 131, 246, 405, 603
Zsp2I ATGCAT 1 cut(s) 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.