MD16G1026300.v1.1

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0016838
Physical Location & Seq
Reverse (-)
1866494 .. 1869637
3144 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1026300.v1.1.491

Sequence Viewer

Length: 1401 bp
ATGGCTGCCCAATCACCATTCGGGTGGTCGTGGAACTCATCGATTCGCGCCACCACCGCCATCAAATGCTCTTCTTCTACTAACTCCTCAACTTTCTCTTCCTTTCACACAAATCCCCGGAAACGACCTCGTTTAACCGCTTCTTCTTATCCTCCTCCTCCTCCTTCTTCTTCTCCTAGTAGTAGCTCTAAACCTAATGCCAAACAGGGACTTAGAAAGCCAGTGAAATTGGATGGTCCGAATTGGAATCCGAACCCAACCACATCGGTTAAAAATTCTAGTGGAAATCGATCGCAAGACGAAAGGAGAGTAACGACCTTGAGCTTCAAGTCCTTGTTCAGCAAACGATCTCTGTGGCGAAGGATCTTTTTCGCTTCCAAGAAAGTTAGGAGCATCATCTTGCTCAACGTCATCACCGTCGTTTATGCCAGTAATTTCTCGATTGTAAAAGAGGTGGAAGCCATCATGGACCCGGCAGCCTTCACTGTTGTGCGGTTTGCTGTGTCAGCCATTCCGTTCATCCCATTTGTGTTCCAAGCTCGAGGTGATGCTCAGACATGTAAAGCAGGGATTGAGTTGGGTTTCTGGGTCAGTCTAGGGTATCTCATGCAGGCACTGGGATTGCAAACTTCAGATGCTGGTCGTGCATCGTTTCTGTCCATGTTCACTGTAGTTATAGTTCCCTTGCTTGATGGTATGTTAGGAGCAATAGTTCCTGCTCGTACCTGGTTTGGAGCTTTTGTGTCTATCATAGGGGTTGCAATGCTGGAATCCAGTGGATCGCCCCCCAGTGTTGGAGACTTGTTGAACTTCTTAAGTGCTGTGTTTTTTGGTGTCCATATGCTAAGAACTGAGCACATTTCGAGAAACACGAAAAGTGAAAATTTCTTAGCTCTGCTCGGTTATGAGGTATGTGTTGTTACTGTCATGTCAGCTATATGGTACTGCGTTGGAGTAGGGTCGGGTGGCCTCCAAGATTTTAACCCATCGTTATGGACGTGGGGGATGTTCTGGGAATGGATGGTTGTGTTCCCATGGATACCTGCACTGTATTCGGGCATATTTTCGACTGGTCTATGCTTATGGGTAGAGATGGCCGCAATGCGTGATGTATCAGCAACAGAAACAGCAATAATTTATAGTTTGGAGCCGGTCTGGGGTGCTGCTTTTGCATGGTTTCTCCTTGGGGAAAGGTGGGGTGCTACTGGTTGGGTTGGCGCTGCTCTGGTGCTAGGTGGAAGCCTAACAGTGCAGATATTTGGATCAGCATCTCCTTCAAAGTCTTACAAAGATGAAAACAATAGTAAGAAACTTGATTACCCGGTGGTTTCAGATAAACAAAATCGCCTTTCTGCCTCTCCGGTTACAGTTAGTTCCAGAAAAGATAGATCTGCTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

467

Amino Acids

50.84

Weight (kDa)

9.8

Isoelectric Point (pI)

43.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 132 - 256 3.6e-09 EamA-like transporter family
EamA PF00892 266 - 415 7.5e-13 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016838)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g32690
malus_domestica MD16G1026300.v1.1
prunus_persica Prupe.1G327200_v2.0.a1
pyrus_communis pycom16g02220
rosa_chinensis RchiOBHm_Chr4g0441611
rosa_laevigata RLG00000006081
rosa_multiflora Rmu_sc0000353.1_g000024
rosa_roxburghii Rroxscaffold_5G00382440
rosa_rugosa Rorug04G0334900
rosa_samantha Rh4AG386400 Rh4BG400600 Rh4CG415600 Rh4DG393300
rosa_wichuraiana Rw4G033380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1051
AccII CGCG 1 cut(s) 48
AciI CCGC 4 cut(s) 57, 138, 493, 1098
AclWI GGATC 3 cut(s) 371, 787, 1270
AcoI YGGCCR 1 cut(s) 1095
AcsI RAATTY 2 cut(s) 274, 883
AcuI CTGAAG 1 cut(s) 615
AfaI GTAC 2 cut(s) 724, 944
AfiI CCNNNNNNNGG 2 cut(s) 794, 1157
AflII CTTAAG 1 cut(s) 814
AflIII ACRYGT 1 cut(s) 557
AgsI TTSAA 3 cut(s) 328, 808, 1278
AjiI CACGTC 1 cut(s) 999
AjnI CCWGG 1 cut(s) 725
AleI CACNNNNGTG 1 cut(s) 488
AloI GAACNNNNNNTCC 2 cut(s) 696, 728
AluBI AGCT 6 cut(s) 186, 324, 539, 737, 893, 935
AluI AGCT 6 cut(s) 186, 324, 539, 737, 893, 935
Alw21I GWGCWC 1 cut(s) 858
Alw26I GTCTC 1 cut(s) 792
AlwI GGATC 3 cut(s) 371, 787, 1270
AlwNI CAGNNNCTG 2 cut(s) 616, 638
Ama87I CYCGRG 1 cut(s) 540
AoxI GGCC 2 cut(s) 967, 1095
ApeKI GCWGC 4 cut(s) 5, 476, 1163, 1220
ApoI RAATTY 2 cut(s) 274, 883
AspLEI GCGC 2 cut(s) 50, 1220
AspS9I GGNCC 2 cut(s) 236, 469
AsuC2I CCSGG 3 cut(s) 118, 473, 1322
AsuHPI GGTGA 3 cut(s) 6, 406, 557
AvaI CYCGRG 1 cut(s) 540
AvaII GGWCC 2 cut(s) 236, 469
Bbv12I GWGCWC 1 cut(s) 858
BbvI GCAGC 3 cut(s) 488, 1150, 1207
BccI CCATC 7 cut(s) 68, 227, 470, 686, 994, 1015, 1087
BcgI CGANNNNNNTGC 2 cut(s) 1035, 1069
BciT130I CCWGG 1 cut(s) 727
BciVI GTATCC 1 cut(s) 1032
BcnI CCSGG 3 cut(s) 118, 473, 1322
BcoDI GTCTC 1 cut(s) 792
BfaI CTAG 4 cut(s) 177, 279, 596, 1232
BfmI CTRYAG 1 cut(s) 669
BfoI RGCGCY 1 cut(s) 1221
BfrI CTTAAG 1 cut(s) 814
BfuAI ACCTGC 1 cut(s) 1051
BfuI GTATCC 1 cut(s) 1032
BglII AGATCT 1 cut(s) 1388
BisI GCNGC 5 cut(s) 6, 477, 1098, 1164, 1221
BlsI GCNGC 5 cut(s) 7, 478, 1099, 1165, 1222
Bme1390I CCNGG 4 cut(s) 118, 473, 727, 1322
Bme18I GGWCC 2 cut(s) 236, 469
BmeT110I CYCGRG 1 cut(s) 540
BmgBI CACGTC 1 cut(s) 999
BmgT120I GGNCC 2 cut(s) 236, 469
BmiI GGNNCC 2 cut(s) 471, 1149
BmrFI CCNGG 4 cut(s) 118, 473, 727, 1322
BmrI ACTGGG 2 cut(s) 626, 783
BmsI GCATC 5 cut(s) 402, 538, 625, 656, 1277
BmuI ACTGGG 2 cut(s) 626, 783
BpuEI CTTGAG 1 cut(s) 340
BpuMI CCSGG 3 cut(s) 118, 473, 1322
Bsa29I ATCGAT 2 cut(s) 41, 289
BsaBI GATNNNNATC 1 cut(s) 1267
BsaJI CCNNGG 3 cut(s) 116, 1034, 1183
BsaWI WCCGGW 1 cut(s) 1360
BsaXI ACNNNNNCTCC 4 cut(s) 696, 726, 726, 756
Bsc4I CCNNNNNNNGG 2 cut(s) 794, 1157
Bse118I RCCGGY 1 cut(s) 1150
Bse1I ACTGG 7 cut(s) 221, 429, 621, 774, 789, 1075, 1210
Bse3DI GCAATG 2 cut(s) 768, 1107
Bse8I GATNNNNATC 1 cut(s) 1267
BseBI CCWGG 1 cut(s) 727
BseCI ATCGAT 2 cut(s) 41, 289
BseDI CCNNGG 3 cut(s) 116, 1034, 1183
BseGI GGATG 4 cut(s) 238, 519, 1011, 1026
BseJI GATNNNNATC 1 cut(s) 1267
BseLI CCNNNNNNNGG 2 cut(s) 794, 1157
BseMI GCAATG 2 cut(s) 768, 1107
BseMII CTCAG 2 cut(s) 566, 843
BseNI ACTGG 7 cut(s) 221, 429, 621, 774, 789, 1075, 1210
BseRI GAGGAG 4 cut(s) 76, 144, 147, 150
BseXI GCAGC 3 cut(s) 488, 1150, 1207
BsgI GTGCAG 2 cut(s) 1029, 1271
Bsh1236I CGCG 1 cut(s) 48
Bsh1285I CGRYCG 1 cut(s) 293
BshFI GGCC 2 cut(s) 969, 1097
BshVI ATCGAT 2 cut(s) 41, 289
BsiEI CGRYCG 1 cut(s) 293
BsiHKAI GWGCWC 1 cut(s) 858
BsiHKCI CYCGRG 1 cut(s) 540
BsiSI CCGG 5 cut(s) 118, 473, 1151, 1322, 1361
BslFI GGGAC 1 cut(s) 222
BslI CCNNNNNNNGG 2 cut(s) 794, 1157
BsmAI GTCTC 1 cut(s) 792
BsmFI GGGAC 1 cut(s) 222
BsnI GGCC 2 cut(s) 969, 1097
BsoBI CYCGRG 1 cut(s) 540
Bsp1286I GDGCHC 1 cut(s) 858
Bsp143I GATC 6 cut(s) 290, 347, 363, 779, 1262, 1388
Bsp19I CCATGG 1 cut(s) 1034
BspACI CCGC 4 cut(s) 57, 138, 493, 1098
BspANI GGCC 2 cut(s) 969, 1097
BspCNI CTCAG 2 cut(s) 565, 844
BspDI ATCGAT 2 cut(s) 41, 289
BspFNI CGCG 1 cut(s) 48
BspLI GGNNCC 2 cut(s) 471, 1149
BspMI ACCTGC 1 cut(s) 1051
BspPI GGATC 3 cut(s) 371, 787, 1270
BspQI GCTCTTC 1 cut(s) 76
BspTI CTTAAG 1 cut(s) 814
BsrDI GCAATG 2 cut(s) 768, 1107
BsrFI RCCGGY 1 cut(s) 1150
BsrI ACTGG 7 cut(s) 221, 429, 621, 774, 789, 1075, 1210
BssAI RCCGGY 1 cut(s) 1150
BssECI CCNNGG 3 cut(s) 116, 1034, 1183
BssMI GATC 6 cut(s) 290, 347, 363, 779, 1262, 1388
BssT1I CCWWGG 2 cut(s) 1034, 1183
Bst2UI CCWGG 1 cut(s) 727
Bst4CI ACNGT 7 cut(s) 418, 487, 670, 925, 1050, 1249, 1369
Bst6I CTCTTC 2 cut(s) 76, 103
BstAFI CTTAAG 1 cut(s) 814
BstC8I GCNNGC 1 cut(s) 612
BstDEI CTNAG 5 cut(s) 212, 552, 845, 852, 889
BstDSI CCRYGG 1 cut(s) 1034
BstF5I GGATG 4 cut(s) 238, 519, 1011, 1026
BstFNI CGCG 1 cut(s) 48
BstH2I RGCGCY 1 cut(s) 1221
BstHHI GCGC 2 cut(s) 50, 1220
BstKTI GATC 6 cut(s) 293, 350, 366, 782, 1265, 1391
BstMAI GTCTC 1 cut(s) 792
BstMBI GATC 6 cut(s) 290, 347, 363, 779, 1262, 1388
BstMCI CGRYCG 1 cut(s) 293
BstMWI GCNNNNNNNGC 4 cut(s) 56, 506, 644, 1169
BstNI CCWGG 1 cut(s) 727
BstNSI RCATGY 1 cut(s) 561
BstSCI CCNGG 4 cut(s) 116, 471, 725, 1320
BstSFI CTRYAG 1 cut(s) 669
BstUI CGCG 1 cut(s) 48
BstV1I GCAGC 3 cut(s) 488, 1150, 1207
BstX2I RGATCY 2 cut(s) 363, 1388
BstXI CCANNNNNNTGG 2 cut(s) 24, 993
BstYI RGATCY 2 cut(s) 363, 1388
Bsu15I ATCGAT 2 cut(s) 41, 289
BsuI GTATCC 1 cut(s) 1032
BsuRI GGCC 2 cut(s) 969, 1097
BsuTUI ATCGAT 2 cut(s) 41, 289
BtgI CCRYGG 1 cut(s) 1034
BtrI CACGTC 1 cut(s) 999
BtsCI GGATG 4 cut(s) 238, 519, 1011, 1026
BtsIMutI CAGTG 8 cut(s) 228, 483, 614, 666, 781, 796, 1046, 1254
BveI ACCTGC 1 cut(s) 1051
Cac8I GCNNGC 1 cut(s) 612
CaiI CAGNNNCTG 2 cut(s) 616, 638
CfoI GCGC 2 cut(s) 50, 1220
Cfr10I RCCGGY 1 cut(s) 1150
Cfr13I GGNCC 2 cut(s) 236, 469
ClaI ATCGAT 2 cut(s) 41, 289
CsiI ACCWGGT 1 cut(s) 725
Csp6I GTAC 2 cut(s) 723, 943
CviAII CATG 7 cut(s) 466, 558, 607, 661, 928, 1035, 1173
CviQI GTAC 2 cut(s) 723, 943
DdeI CTNAG 5 cut(s) 212, 552, 845, 852, 889
DpnI GATC 6 cut(s) 292, 349, 365, 781, 1264, 1390
DpnII GATC 6 cut(s) 290, 347, 363, 779, 1262, 1388
EaeI YGGCCR 1 cut(s) 1095
Eam1104I CTCTTC 2 cut(s) 76, 103
EarI CTCTTC 2 cut(s) 76, 103
Eco130I CCWWGG 2 cut(s) 1034, 1183
Eco47I GGWCC 2 cut(s) 236, 469
Eco57I CTGAAG 1 cut(s) 615
Eco88I CYCGRG 1 cut(s) 540
EcoRII CCWGG 1 cut(s) 725
EcoT14I CCWWGG 2 cut(s) 1034, 1183
ErhI CCWWGG 2 cut(s) 1034, 1183
FaeI CATG 7 cut(s) 469, 561, 610, 664, 931, 1038, 1176
FaqI GGGAC 1 cut(s) 222
FatI CATG 7 cut(s) 465, 557, 606, 660, 927, 1034, 1172
FauNDI CATATG 1 cut(s) 840
Fnu4HI GCNGC 5 cut(s) 6, 477, 1098, 1164, 1221
FokI GGATG 4 cut(s) 245, 506, 1018, 1033
Fsp4HI GCNGC 5 cut(s) 6, 477, 1098, 1164, 1221
FspBI CTAG 4 cut(s) 177, 279, 596, 1232
GlaI GCGC 2 cut(s) 49, 1219
GluI GCNGC 5 cut(s) 6, 477, 1098, 1164, 1221
HaeII RGCGCY 1 cut(s) 1221
HaeIII GGCC 2 cut(s) 969, 1097
HapII CCGG 5 cut(s) 118, 473, 1151, 1322, 1361
HhaI GCGC 2 cut(s) 50, 1220
Hin1II CATG 7 cut(s) 469, 561, 610, 664, 931, 1038, 1176
Hin6I GCGC 2 cut(s) 48, 1218
HinP1I GCGC 2 cut(s) 48, 1218
HinfI GANTC 3 cut(s) 43, 247, 770
HpaII CCGG 5 cut(s) 118, 473, 1151, 1322, 1361
HphI GGTGA 3 cut(s) 6, 406, 557
Hpy166II GTNNAC 1 cut(s) 666
Hpy188I TCNGA 5 cut(s) 240, 252, 555, 634, 1333
Hpy188III TCNNGA 3 cut(s) 439, 864, 1377
Hpy8I GTNNAC 1 cut(s) 666
Hpy99I CGWCG 1 cut(s) 422
HpyAV CCTTC 4 cut(s) 174, 354, 490, 1284
HpyCH4III ACNGT 7 cut(s) 418, 487, 670, 925, 1050, 1249, 1369
HpyCH4IV ACGT 2 cut(s) 408, 998
HpyCH4V TGCA 7 cut(s) 610, 625, 647, 761, 1046, 1172, 1252
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 506, 644, 1169
HpyF3I CTNAG 5 cut(s) 212, 552, 845, 852, 889
HpySE526I ACGT 2 cut(s) 408, 998
Hsp92II CATG 7 cut(s) 469, 561, 610, 664, 931, 1038, 1176
HspAI GCGC 2 cut(s) 48, 1218
Kzo9I GATC 6 cut(s) 290, 347, 363, 779, 1262, 1388
LguI GCTCTTC 1 cut(s) 76
LmnI GCTCC 4 cut(s) 390, 704, 734, 1147
Lsp1109I GCAGC 3 cut(s) 488, 1150, 1207
LweI GCATC 5 cut(s) 402, 538, 625, 656, 1277
MabI ACCWGGT 1 cut(s) 725
MaeI CTAG 4 cut(s) 177, 279, 596, 1232
MaeII ACGT 2 cut(s) 408, 998
MaeIII GTNAC 3 cut(s) 310, 919, 1363
MalI GATC 6 cut(s) 292, 349, 365, 781, 1264, 1390
MboI GATC 6 cut(s) 290, 347, 363, 779, 1262, 1388
MboII GAAGA 6 cut(s) 63, 66, 90, 135, 159, 162
MflI RGATCY 2 cut(s) 363, 1388
MhlI GDGCHC 1 cut(s) 858
MluCI AATT 6 cut(s) 227, 241, 274, 433, 883, 1134
MmeI TCCRAC 2 cut(s) 775, 931
MseI TTAA 4 cut(s) 134, 270, 815, 981
MslI CAYNNNNRTG 3 cut(s) 22, 488, 991
MspCI CTTAAG 1 cut(s) 814
MspI CCGG 5 cut(s) 118, 473, 1151, 1322, 1361
MspR9I CCNGG 4 cut(s) 118, 473, 727, 1322
MvaI CCWGG 1 cut(s) 727
MvnI CGCG 1 cut(s) 48
MwoI GCNNNNNNNGC 4 cut(s) 56, 506, 644, 1169
NciI CCSGG 3 cut(s) 118, 473, 1322
NcoI CCATGG 1 cut(s) 1034
NdeI CATATG 1 cut(s) 840
NdeII GATC 6 cut(s) 290, 347, 363, 779, 1262, 1388
NlaIII CATG 7 cut(s) 469, 561, 610, 664, 931, 1038, 1176
NlaIV GGNNCC 2 cut(s) 471, 1149
NspI RCATGY 1 cut(s) 561
OliI CACNNNNGTG 1 cut(s) 488
PaeR7I CTCGAG 1 cut(s) 540
PciI ACATGT 1 cut(s) 557
PciSI GCTCTTC 1 cut(s) 76
PcsI WCGNNNNNNNCGW 3 cut(s) 414, 869, 995
PfeI GAWTC 3 cut(s) 43, 247, 770
PkrI GCNGC 5 cut(s) 7, 478, 1099, 1165, 1222
Ple19I CGATCG 1 cut(s) 293
PscI ACATGT 1 cut(s) 557
Psp6I CCWGG 1 cut(s) 725
PspGI CCWGG 1 cut(s) 725
PspN4I GGNNCC 2 cut(s) 471, 1149
PspPI GGNCC 2 cut(s) 236, 469
PspXI VCTCGAGB 1 cut(s) 540
PsrI GAACNNNNNNTAC 2 cut(s) 663, 695
PstNI CAGNNNCTG 2 cut(s) 616, 638
PsuI RGATCY 2 cut(s) 363, 1388
PvuI CGATCG 1 cut(s) 293
RsaI GTAC 2 cut(s) 724, 944
RsaNI GTAC 2 cut(s) 723, 943
RseI CAYNNNNRTG 3 cut(s) 22, 488, 991
SapI GCTCTTC 1 cut(s) 76
SaqAI TTAA 4 cut(s) 134, 270, 815, 981
SatI GCNGC 5 cut(s) 6, 477, 1098, 1164, 1221
Sau3AI GATC 6 cut(s) 290, 347, 363, 779, 1262, 1388
Sau96I GGNCC 2 cut(s) 236, 469
ScrFI CCNGG 4 cut(s) 118, 473, 727, 1322
SduI GDGCHC 1 cut(s) 858
SexAI ACCWGGT 1 cut(s) 725
SfaNI GCATC 5 cut(s) 402, 538, 625, 656, 1277
SfcI CTRYAG 1 cut(s) 669
Sfr274I CTCGAG 1 cut(s) 540
SinI GGWCC 2 cut(s) 236, 469
SlaI CTCGAG 1 cut(s) 540
SmiMI CAYNNNNRTG 3 cut(s) 22, 488, 991
SmlI CTYRAG 3 cut(s) 319, 540, 814
SmoI CTYRAG 3 cut(s) 319, 540, 814
Sse9I AATT 6 cut(s) 227, 241, 274, 433, 883, 1134
SsiI CCGC 4 cut(s) 57, 138, 493, 1098
SspMI CTAG 4 cut(s) 177, 279, 596, 1232
StyD4I CCNGG 4 cut(s) 116, 471, 725, 1320
StyI CCWWGG 2 cut(s) 1034, 1183
TaaI ACNGT 7 cut(s) 418, 487, 670, 925, 1050, 1249, 1369
TaiI ACGT 2 cut(s) 411, 1001
TaqI TCGA 6 cut(s) 41, 289, 440, 541, 863, 1067
TasI AATT 6 cut(s) 227, 241, 274, 433, 883, 1134
TauI GCSGC 1 cut(s) 1100
TfiI GAWTC 3 cut(s) 43, 247, 770
Tru1I TTAA 4 cut(s) 134, 270, 815, 981
Tru9I TTAA 4 cut(s) 134, 270, 815, 981
TscAI CASTG 8 cut(s) 228, 490, 621, 673, 781, 796, 1053, 1254
TseI GCWGC 4 cut(s) 5, 476, 1163, 1220
TspDTI ATGAA 2 cut(s) 508, 1308
TspGWI ACGGA 1 cut(s) 504
TspRI CASTG 8 cut(s) 228, 490, 621, 673, 781, 796, 1053, 1254
Vha464I CTTAAG 1 cut(s) 814
VpaK11BI GGWCC 2 cut(s) 236, 469
XapI RAATTY 2 cut(s) 274, 883
XceI RCATGY 1 cut(s) 561
XhoI CTCGAG 1 cut(s) 540
XspI CTAG 4 cut(s) 177, 279, 596, 1232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.