Rorug04G0334900

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0016838
Physical Location & Seq
Forward (+)
50922143 .. 50923813
1671 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0334900.1

Sequence Viewer

Length: 1311 bp
ATGGCTACGGTGCGGCTCGAAGTAATATCCAGAGAGCTCATCAAACCCTCCTCTCAAACTCCACATCACCTAAGAGACTACAACCTCTCCTACTTCGATCAGCTCTCTCCTCGTAAGTACATCCCGCTTGTTCTTTTCTATAACCCTTGCAATACCACTGCTAGCACTTTTGAGACATCCATTCTCCTCAAAAAATCTTTATCTGAAACCCTAGCCTTTTACTACCCATTTGCCGGAAAGGTCAAAGATCAAGAGTTCATCGACTGCAACGACGAGGGAATTTTGTTCTTGGAAGCAAAGGTCAAACTGAAGTTGTCTGAAATTCTTGAGAATCCGAAACATGAAATTTTGGACCTTTTATTTGCAGACAATTTGCAGTGGAATGATACGAATTTGAGTACTTTACTTGCTGTACAAGTCAGCTACTTTGACTGTGGGGGAATGGCATTAAGTGTGTGCATGTCACACAAAATTGGCGATGCAGCAACCATGGTCAATTTCGTCAATGACTGGGCTGCCAAGGCTTCAAACTCAGGTAAACAAGTTTCCCCTTTGCTCTATACACCCTCGATTTTTCCAAGGGGTGACTTACCACTTATGCCGCAGCCGGAATATTCCATCAAGAAAGAAAATTGTGCCTCCAGAAGGTTTGTATTCGATGCCCAAAAAATTGCGGCCCTCAAAGCCATTGTAGCTGACAAAGTGCAAAAGGCTACAAAGGTAGAGGTTGTGAGTGCTCTACTATTTAAGTGTGCAATTTCTGCCGCCAATGCAAGTTCATCAGGTACTACAAGACCAACCCTTTTTCTCCAAAATGTTAACCTTCGTACCAGGATGATTCCTCCGCTACCAGAAAACTATGTGGGTAACATCAGTTGGGTATTTCCGGTACCAAAAAGGGAGGACATCAGTTTGCATTGCTTGGTGGGTCAAATGAAAGAGGCCTTGTCAGATTTCTGCAACACATATGTTAAGAATGTAAGGGGTACAGAATTGGCCTTGAGCATTAGCAAAACCATGGAGAAGATGCGGGCTATGTTCAAAAGCAGTAAGCAATGTGTGCAATACAATTGTAGTAGCTGGTGCAGGTTCCCAGTATACGAAGCAGATTTTGGGTGGGGAAAGCCTGTGTGGGTGAGTATTGGGGCTTGTCTTGACAAGAATGTTATAATTCTGATGGATAGTAAAAGTGGGGATGGGATCGAAGCTTTTGTCACTCTGGAAGAGCAAGACATGGCTGCTTTTGAGTGTGATGAGGAGTTACTAGCATTTGCTGCTCTAAATCCAACTCCATTAACAAGCCAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

436

Amino Acids

48.71

Weight (kDa)

6.27

Isoelectric Point (pI)

38.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 421 1.3e-86 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016838)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g32690
malus_domestica MD16G1026300.v1.1
prunus_persica Prupe.1G327200_v2.0.a1
pyrus_communis pycom16g02220
rosa_chinensis RchiOBHm_Chr4g0441611
rosa_laevigata RLG00000006081
rosa_multiflora Rmu_sc0000353.1_g000024
rosa_roxburghii Rroxscaffold_5G00382440
rosa_rugosa Rorug04G0334900
rosa_samantha Rh4AG386400 Rh4BG400600 Rh4CG415600 Rh4DG393300
rosa_wichuraiana Rw4G033380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1169
Acc36I ACCTGC 1 cut(s) 1077
Acc65I GGTACC 1 cut(s) 889
AccB1I GGYRCC 1 cut(s) 889
AccI GTMKAC 1 cut(s) 1098
AciI CCGC 7 cut(s) 13, 125, 602, 674, 765, 845, 1030
AclWI GGATC 1 cut(s) 1208
AcsI RAATTY 4 cut(s) 279, 321, 345, 391
AcuI CTGAAG 1 cut(s) 329
AfaI GTAC 7 cut(s) 119, 400, 414, 787, 829, 891, 988
AfiI CCNNNNNNNGG 2 cut(s) 233, 645
AgsI TTSAA 2 cut(s) 528, 1042
AjnI CCWGG 1 cut(s) 830
AjuI GAANNNNNNNTTGG 2 cut(s) 1095, 1127
AluBI AGCT 6 cut(s) 37, 103, 423, 695, 1080, 1208
AluI AGCT 6 cut(s) 37, 103, 423, 695, 1080, 1208
Alw21I GWGCWC 2 cut(s) 39, 739
Alw26I GTCTC 2 cut(s) 69, 167
AlwI GGATC 1 cut(s) 1208
AoxI GGCC 3 cut(s) 675, 942, 996
ApeKI GCWGC 5 cut(s) 482, 515, 604, 1238, 1274
ApoI RAATTY 4 cut(s) 279, 321, 345, 391
ArsI GACNNNNNNTTYG 4 cut(s) 344, 376, 1197, 1229
Asp718I GGTACC 1 cut(s) 889
AspS9I GGNCC 2 cut(s) 352, 676
AsuHPI GGTGA 3 cut(s) 59, 596, 1147
AsuNHI GCTAGC 1 cut(s) 161
AvaII GGWCC 1 cut(s) 352
BaeI ACNNNNGTAYC 2 cut(s) 811, 844
BanI GGYRCC 1 cut(s) 889
BanII GRGCYC 1 cut(s) 39
Bbv12I GWGCWC 2 cut(s) 39, 739
BbvI GCAGC 5 cut(s) 494, 502, 616, 1225, 1261
BccI CCATC 3 cut(s) 626, 1171, 1190
BciT130I CCWGG 1 cut(s) 832
BcoDI GTCTC 2 cut(s) 69, 167
BfaI CTAG 3 cut(s) 162, 212, 1265
BfuAI ACCTGC 1 cut(s) 1077
BisI GCNGC 9 cut(s) 14, 483, 516, 602, 605, 675, 765, 1239, 1275
BlsI GCNGC 9 cut(s) 15, 484, 517, 603, 606, 676, 766, 1240, 1276
BmcAI AGTACT 1 cut(s) 400
Bme1390I CCNGG 1 cut(s) 832
Bme18I GGWCC 1 cut(s) 352
BmgT120I GGNCC 2 cut(s) 352, 676
BmiI GGNNCC 2 cut(s) 891, 1091
BmrFI CCNGG 1 cut(s) 832
BmrI ACTGGG 2 cut(s) 520, 1088
BmsI GCATC 3 cut(s) 469, 649, 1017
BmtI GCTAGC 1 cut(s) 165
BmuI ACTGGG 2 cut(s) 520, 1088
BpmI CTGGAG 1 cut(s) 625
BpuEI CTTGAG 2 cut(s) 347, 1021
BsaJI CCNNGG 4 cut(s) 489, 519, 578, 1017
BsaWI WCCGGW 1 cut(s) 886
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 645
Bse1I ACTGG 2 cut(s) 515, 1094
Bse3DI GCAATG 2 cut(s) 916, 1061
BseBI CCWGG 1 cut(s) 832
BseDI CCNNGG 4 cut(s) 489, 519, 578, 1017
BseGI GGATG 4 cut(s) 120, 176, 840, 1201
BseLI CCNNNNNNNGG 2 cut(s) 233, 645
BseMI GCAATG 2 cut(s) 916, 1061
BseMII CTCAG 1 cut(s) 546
BseNI ACTGG 2 cut(s) 515, 1094
BseRI GAGGAG 4 cut(s) 40, 99, 176, 1271
BseXI GCAGC 5 cut(s) 494, 502, 616, 1225, 1261
BsgI GTGCAG 1 cut(s) 1105
BshFI GGCC 3 cut(s) 677, 944, 998
BshNI GGYRCC 1 cut(s) 889
BsiHKAI GWGCWC 2 cut(s) 39, 739
BsiSI CCGG 3 cut(s) 234, 608, 887
BslI CCNNNNNNNGG 2 cut(s) 233, 645
BsmAI GTCTC 2 cut(s) 69, 167
BsnI GGCC 3 cut(s) 677, 944, 998
Bsp1286I GDGCHC 2 cut(s) 39, 739
Bsp1407I TGTACA 1 cut(s) 412
Bsp143I GATC 3 cut(s) 97, 247, 1200
Bsp19I CCATGG 2 cut(s) 489, 1017
BspACI CCGC 7 cut(s) 13, 125, 602, 674, 765, 845, 1030
BspANI GGCC 3 cut(s) 677, 944, 998
BspCNI CTCAG 1 cut(s) 545
BspLI GGNNCC 2 cut(s) 891, 1091
BspMI ACCTGC 1 cut(s) 1077
BspOI GCTAGC 1 cut(s) 165
BspPI GGATC 1 cut(s) 1208
BspQI GCTCTTC 1 cut(s) 1218
BspT107I GGYRCC 1 cut(s) 889
BsrDI GCAATG 2 cut(s) 916, 1061
BsrGI TGTACA 1 cut(s) 412
BsrI ACTGG 2 cut(s) 515, 1094
BssECI CCNNGG 4 cut(s) 489, 519, 578, 1017
BssMI GATC 3 cut(s) 97, 247, 1200
BssNAI GTATAC 1 cut(s) 1099
BssT1I CCWWGG 4 cut(s) 489, 519, 578, 1017
Bst1107I GTATAC 1 cut(s) 1099
Bst2UI CCWGG 1 cut(s) 832
Bst4CI ACNGT 2 cut(s) 10, 434
Bst6I CTCTTC 1 cut(s) 1218
BstAPI GCANNNNNTGC 3 cut(s) 761, 1060, 1274
BstAUI TGTACA 1 cut(s) 412
BstC8I GCNNGC 2 cut(s) 163, 1032
BstDEI CTNAG 2 cut(s) 71, 532
BstDSI CCRYGG 2 cut(s) 489, 1017
BstENI CCTNNNNNAGG 1 cut(s) 643
BstF5I GGATG 4 cut(s) 120, 176, 840, 1201
BstKTI GATC 3 cut(s) 100, 250, 1203
BstMAI GTCTC 2 cut(s) 69, 167
BstMBI GATC 3 cut(s) 97, 247, 1200
BstMWI GCNNNNNNNGC 7 cut(s) 521, 683, 692, 761, 770, 1060, 1274
BstNI CCWGG 1 cut(s) 832
BstNSI RCATGY 1 cut(s) 463
BstSCI CCNGG 1 cut(s) 830
BstV1I GCAGC 5 cut(s) 494, 502, 616, 1225, 1261
BstZ17I GTATAC 1 cut(s) 1099
BsuRI GGCC 3 cut(s) 677, 944, 998
BtgI CCRYGG 2 cut(s) 489, 1017
BtgZI GCGATG 1 cut(s) 492
BtsCI GGATG 4 cut(s) 120, 176, 840, 1201
BtsI GCAGTG 2 cut(s) 156, 383
BtsIMutI CAGTG 2 cut(s) 156, 383
BveI ACCTGC 1 cut(s) 1077
Cac8I GCNNGC 2 cut(s) 163, 1032
Cfr13I GGNCC 2 cut(s) 352, 676
Csp6I GTAC 7 cut(s) 118, 399, 413, 786, 828, 890, 987
CviAII CATG 5 cut(s) 341, 460, 490, 1018, 1234
CviQI GTAC 7 cut(s) 118, 399, 413, 786, 828, 890, 987
DdeI CTNAG 2 cut(s) 71, 532
DpnI GATC 3 cut(s) 99, 249, 1202
DpnII GATC 3 cut(s) 97, 247, 1200
Eam1104I CTCTTC 1 cut(s) 1218
EarI CTCTTC 1 cut(s) 1218
Ecl136II GAGCTC 1 cut(s) 37
Eco130I CCWWGG 4 cut(s) 489, 519, 578, 1017
Eco147I AGGCCT 1 cut(s) 944
Eco24I GRGCYC 1 cut(s) 39
Eco47I GGWCC 1 cut(s) 352
Eco53kI GAGCTC 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 329
EcoICRI GAGCTC 1 cut(s) 37
EcoNI CCTNNNNNAGG 1 cut(s) 643
EcoRII CCWGG 1 cut(s) 830
EcoT14I CCWWGG 4 cut(s) 489, 519, 578, 1017
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 4 cut(s) 489, 519, 578, 1017
FaeI CATG 5 cut(s) 344, 463, 493, 1021, 1237
FatI CATG 5 cut(s) 340, 459, 489, 1017, 1233
FauI CCCGC 2 cut(s) 132, 1023
FauNDI CATATG 1 cut(s) 967
FblI GTMKAC 1 cut(s) 1098
Fnu4HI GCNGC 9 cut(s) 14, 483, 516, 602, 605, 675, 765, 1239, 1275
FokI GGATG 4 cut(s) 107, 163, 847, 1208
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 9 cut(s) 14, 483, 516, 602, 605, 675, 765, 1239, 1275
FspBI CTAG 3 cut(s) 162, 212, 1265
GluI GCNGC 9 cut(s) 14, 483, 516, 602, 605, 675, 765, 1239, 1275
GsuI CTGGAG 1 cut(s) 625
HaeIII GGCC 3 cut(s) 677, 944, 998
HapII CCGG 3 cut(s) 234, 608, 887
Hin1II CATG 5 cut(s) 344, 463, 493, 1021, 1237
HincII GTYRAC 1 cut(s) 820
HindII GTYRAC 1 cut(s) 820
HindIII AAGCTT 1 cut(s) 1206
HinfI GANTC 2 cut(s) 331, 838
HpaI GTTAAC 1 cut(s) 820
HpaII CCGG 3 cut(s) 234, 608, 887
HphI GGTGA 3 cut(s) 59, 596, 1147
Hpy166II GTNNAC 3 cut(s) 539, 820, 1099
Hpy188I TCNGA 5 cut(s) 205, 319, 336, 952, 1176
Hpy188III TCNNGA 7 cut(s) 30, 251, 326, 622, 642, 1154, 1220
Hpy8I GTNNAC 3 cut(s) 539, 820, 1099
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 2 cut(s) 639, 833
HpyCH4III ACNGT 2 cut(s) 10, 434
HpyF10VI GCNNNNNNNGC 7 cut(s) 521, 683, 692, 761, 770, 1060, 1274
HpyF3I CTNAG 2 cut(s) 71, 532
Hsp92II CATG 5 cut(s) 344, 463, 493, 1021, 1237
KpnI GGTACC 1 cut(s) 893
KspAI GTTAAC 1 cut(s) 820
Kzo9I GATC 3 cut(s) 97, 247, 1200
LguI GCTCTTC 1 cut(s) 1218
Lsp1109I GCAGC 5 cut(s) 494, 502, 616, 1225, 1261
LweI GCATC 3 cut(s) 469, 649, 1017
MaeI CTAG 3 cut(s) 162, 212, 1265
MaeIII GTNAC 5 cut(s) 462, 584, 866, 1213, 1260
MalI GATC 3 cut(s) 99, 249, 1202
MboI GATC 3 cut(s) 97, 247, 1200
MboII GAAGA 2 cut(s) 1036, 1235
MfeI CAATTG 1 cut(s) 1069
MhlI GDGCHC 2 cut(s) 39, 739
MmeI TCCRAC 1 cut(s) 1310
MseI TTAA 5 cut(s) 449, 747, 819, 972, 1295
MspI CCGG 3 cut(s) 234, 608, 887
MspR9I CCNGG 1 cut(s) 832
MunI CAATTG 1 cut(s) 1069
MvaI CCWGG 1 cut(s) 832
MwoI GCNNNNNNNGC 7 cut(s) 521, 683, 692, 761, 770, 1060, 1274
NcoI CCATGG 2 cut(s) 489, 1017
NdeI CATATG 1 cut(s) 967
NdeII GATC 3 cut(s) 97, 247, 1200
NheI GCTAGC 1 cut(s) 161
NlaIII CATG 5 cut(s) 344, 463, 493, 1021, 1237
NlaIV GGNNCC 2 cut(s) 891, 1091
NmuCI GTSAC 3 cut(s) 462, 584, 1213
NspI RCATGY 1 cut(s) 463
PceI AGGCCT 1 cut(s) 944
PciSI GCTCTTC 1 cut(s) 1218
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PfeI GAWTC 2 cut(s) 331, 838
PkrI GCNGC 9 cut(s) 15, 484, 517, 603, 606, 676, 766, 1240, 1276
PsiI TTATAA 1 cut(s) 1169
Psp124BI GAGCTC 1 cut(s) 39
Psp6I CCWGG 1 cut(s) 830
PspGI CCWGG 1 cut(s) 830
PspN4I GGNNCC 2 cut(s) 891, 1091
PspPI GGNCC 2 cut(s) 352, 676
RsaI GTAC 7 cut(s) 119, 400, 414, 787, 829, 891, 988
RsaNI GTAC 7 cut(s) 118, 399, 413, 786, 828, 890, 987
SacI GAGCTC 1 cut(s) 39
SapI GCTCTTC 1 cut(s) 1218
SaqAI TTAA 5 cut(s) 449, 747, 819, 972, 1295
SatI GCNGC 9 cut(s) 14, 483, 516, 602, 605, 675, 765, 1239, 1275
Sau3AI GATC 3 cut(s) 97, 247, 1200
Sau96I GGNCC 2 cut(s) 352, 676
ScaI AGTACT 1 cut(s) 400
ScrFI CCNGG 1 cut(s) 832
SduI GDGCHC 2 cut(s) 39, 739
SfaNI GCATC 3 cut(s) 469, 649, 1017
SinI GGWCC 1 cut(s) 352
SmlI CTYRAG 2 cut(s) 326, 1000
SmoI CTYRAG 2 cut(s) 326, 1000
SseBI AGGCCT 1 cut(s) 944
SsiI CCGC 7 cut(s) 13, 125, 602, 674, 765, 845, 1030
SspI AATATT 1 cut(s) 614
SspMI CTAG 3 cut(s) 162, 212, 1265
SstI GAGCTC 1 cut(s) 39
StuI AGGCCT 1 cut(s) 944
StyD4I CCNGG 1 cut(s) 830
StyI CCWWGG 4 cut(s) 489, 519, 578, 1017
TaaI ACNGT 2 cut(s) 10, 434
TaqI TCGA 6 cut(s) 18, 96, 261, 569, 657, 1203
TatI WGTACW 3 cut(s) 117, 398, 412
TauI GCSGC 4 cut(s) 16, 604, 677, 767
TfiI GAWTC 2 cut(s) 331, 838
Tru1I TTAA 5 cut(s) 449, 747, 819, 972, 1295
Tru9I TTAA 5 cut(s) 449, 747, 819, 972, 1295
TscAI CASTG 2 cut(s) 163, 383
TseFI GTSAC 3 cut(s) 462, 584, 1213
TseI GCWGC 5 cut(s) 482, 515, 604, 1238, 1274
Tsp45I GTSAC 3 cut(s) 462, 584, 1213
TspDTI ATGAA 4 cut(s) 247, 357, 768, 950
TspRI CASTG 2 cut(s) 163, 383
VpaK11BI GGWCC 1 cut(s) 352
XagI CCTNNNNNAGG 1 cut(s) 643
XapI RAATTY 4 cut(s) 279, 321, 345, 391
XceI RCATGY 1 cut(s) 463
XmiI GTMKAC 1 cut(s) 1098
XspI CTAG 3 cut(s) 162, 212, 1265
ZrmI AGTACT 1 cut(s) 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.