RchiOBHm_Chr4g0441611

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0016838
Physical Location & Seq
Forward (+)
63407291 .. 63410520
3230 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40942

Sequence Viewer

Length: 1374 bp
ATGGCTGCCCAATCACCGTGTGGGTGGTCATGGAGCTCATCCACCTACGACGCCACCACCGCCAAATGCTCTTCCAGCTCATCGAATCTCTCTTCGTTTTATACAAAACGACGACCTCGTTTCACCGCCTGTTCGTCGTCTTCTAAATCACCCGGTAGCTCCGGCGCCAAAGCGCGGCTGAAAAAGCCGGCGAAATTGGACAGTACCGGTCCGAGTCCAACCGCATTGGTGAAAACCGCAAAGGGAGATGGACCCAGAAGTGAAAAGACGGTAACAGCCCTGAGCTTCAAGTCCTTGTCTGGGAAGCGATCTCTATGGCGGAGGATATTCTTCGCCTCCAAGAAAGTTAGGAGCATCCTTCTGCTTAATGTAATCACTGTAGTCTATGCTAGTAATTTTTCGATTGTGAAAGAAGTGGAAGCTGTGATAGACCCGGCGGCGTTTACTGTTGTGCGGTTTGCTATGTCGGCGGTTCCATTTATCCCATTTGTTTTCCGAGCAAGAGGTGATGTTGATACTCGTAATGCAGGAATTGAGTTGGGATTCTGGGTTAGTTTAGGGTATCTTATGCAGGCATTAGGACTGCTAACATCTGATGCTGGTCGTGCGTCATTTCTTTCTATGTTCACTGTGATTATCGTTCCCTTGCTTGATGGAATGTTAGGAGCAATAGTCCCTGCTCGTACCTGGTTTGGAGCCCTCATGTCTATTGTCGGAGTTGGGCTGCTGGAATCCAGTGGATCTCCCCCAAGTGTTGGAGATTTGCTGAACTTCTTGAGCGCGGTGTTTTTTGGGGTTCATATGCTTAGAACAGAGCATATATCAAGAAACACAAAAAAAGAAAACTTCTTGCCTCTGCTTGGATATGAGATATGCGTTGTTGCTTTCTTATCAACCATATGGTACCTCATTGGGGCATGGTCTGGTGGTGGCCTCCACGACTTTAACCCATCATTGTGGACAGGGGAGATGTTCTGGGAGTGGATGGTTGTGTTTCCATGGATACCTGCACTGTACACAGGCATTTTCTCCACTGTTTTATGCTTATGGGTAGAGATGGGCGCAATGCGCGATGTATCAGCCACAGAAACGGCCATAATTTATGGTTTGGAGCCTGTCTGGGGTGCTGGTTTCGCATGGTTTCTCCTTGGCGAAAGATGGGGTTCTACTGGTTGGATTGGTGCTGCTCTTGTGCTAGGTGGAAGCTTAACAGTGCAGATATTTGGATCAACATCTCCCGCAGAGCCTTCAAAAGATGAAATGAAAGCTAAGAAACTCACTTTCCCAGTGGTTTCGGATAAACACAATGGCTTATCTGCCTCGCCAGTTATAGTCAGTTCCAGAAAGGATGCTCCTGATCTGCGGAGTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

457

Amino Acids

49.39

Weight (kDa)

9.59

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 119 - 242 9.4e-08 EamA-like transporter family
EamA PF00892 253 - 403 1.5e-12 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016838)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g32690
malus_domestica MD16G1026300.v1.1
prunus_persica Prupe.1G327200_v2.0.a1
pyrus_communis pycom16g02220
rosa_chinensis RchiOBHm_Chr4g0441611
rosa_laevigata RLG00000006081
rosa_multiflora Rmu_sc0000353.1_g000024
rosa_roxburghii Rroxscaffold_5G00382440
rosa_rugosa Rorug04G0334900
rosa_samantha Rh4AG386400 Rh4BG400600 Rh4CG415600 Rh4DG393300
rosa_wichuraiana Rw4G033380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1015
Acc65I GGTACC 1 cut(s) 903
AccB1I GGYRCC 2 cut(s) 164, 903
AccB7I CCANNNNNTGG 1 cut(s) 755
AccII CGCG 3 cut(s) 175, 782, 1071
AclWI GGATC 2 cut(s) 748, 1234
AcoI YGGCCR 1 cut(s) 1092
AcyI GRCGYC 2 cut(s) 51, 165
AdeI CACNNNGTG 1 cut(s) 20
AfaI GTAC 4 cut(s) 205, 685, 905, 1016
AfiI CCNNNNNNNGG 6 cut(s) 174, 300, 755, 860, 913, 1121
AgeI ACCGGT 1 cut(s) 206
AgsI TTSAA 2 cut(s) 289, 1251
AjnI CCWGG 1 cut(s) 686
AluBI AGCT 7 cut(s) 36, 78, 159, 285, 422, 1206, 1268
AluI AGCT 7 cut(s) 36, 78, 159, 285, 422, 1206, 1268
Alw21I GWGCWC 1 cut(s) 38
AlwI GGATC 2 cut(s) 748, 1234
AoxI GGCC 2 cut(s) 931, 1092
ApeKI GCWGC 3 cut(s) 5, 724, 1184
AsiGI ACCGGT 1 cut(s) 206
Asp718I GGTACC 1 cut(s) 903
AspLEI GCGC 5 cut(s) 167, 175, 782, 1064, 1071
AspS9I GGNCC 2 cut(s) 209, 251
AsuC2I CCSGG 2 cut(s) 153, 434
AsuHPI GGTGA 5 cut(s) 6, 115, 141, 241, 518
AvaII GGWCC 2 cut(s) 209, 251
BanI GGYRCC 2 cut(s) 164, 903
BanII GRGCYC 2 cut(s) 38, 700
BbsI GAAGAC 1 cut(s) 132
Bbv12I GWGCWC 1 cut(s) 38
BbvI GCAGC 2 cut(s) 711, 1171
BccI CCATC 6 cut(s) 242, 647, 958, 979, 1051, 1152
BceAI ACGGC 1 cut(s) 1107
BciT130I CCWGG 1 cut(s) 688
BciVI GTATCC 1 cut(s) 996
BcnI CCSGG 2 cut(s) 153, 434
BfaI CTAG 2 cut(s) 390, 1196
BfmI CTRYAG 1 cut(s) 378
BfoI RGCGCY 1 cut(s) 168
BfuAI ACCTGC 1 cut(s) 1015
BfuI GTATCC 1 cut(s) 996
BisI GCNGC 5 cut(s) 6, 176, 438, 725, 1185
BlsI GCNGC 5 cut(s) 7, 177, 439, 726, 1186
Bme1390I CCNGG 3 cut(s) 153, 434, 688
Bme18I GGWCC 2 cut(s) 209, 251
BmgT120I GGNCC 2 cut(s) 209, 251
BmiI GGNNCC 6 cut(s) 166, 253, 474, 697, 905, 1113
BmrFI CCNGG 3 cut(s) 153, 434, 688
BmrI ACTGGG 1 cut(s) 1280
BmsI GCATC 3 cut(s) 363, 586, 1339
BmuI ACTGGG 1 cut(s) 1280
BpiI GAAGAC 1 cut(s) 132
Bpu10I CCTNAGC 1 cut(s) 281
BpuEI CTTGAG 1 cut(s) 796
BpuMI CCSGG 2 cut(s) 153, 434
BsaBI GATNNNNATC 1 cut(s) 1231
BsaHI GRCGYC 2 cut(s) 51, 165
BsaJI CCNNGG 2 cut(s) 998, 1147
BsaWI WCCGGW 1 cut(s) 206
BsaXI ACNNNNNCTCC 2 cut(s) 657, 687
Bsc4I CCNNNNNNNGG 6 cut(s) 174, 300, 755, 860, 913, 1121
Bse118I RCCGGY 2 cut(s) 187, 206
Bse1I ACTGG 4 cut(s) 735, 1174, 1286, 1325
Bse3DI GCAATG 1 cut(s) 1071
Bse8I GATNNNNATC 1 cut(s) 1231
BseBI CCWGG 1 cut(s) 688
BseDI CCNNGG 2 cut(s) 998, 1147
BseGI GGATG 4 cut(s) 38, 354, 990, 1354
BseJI GATNNNNATC 1 cut(s) 1231
BseLI CCNNNNNNNGG 6 cut(s) 174, 300, 755, 860, 913, 1121
BseMI GCAATG 1 cut(s) 1071
BseMII CTCAG 1 cut(s) 272
BseNI ACTGG 4 cut(s) 735, 1174, 1286, 1325
BseXI GCAGC 2 cut(s) 711, 1171
BsgI GTGCAG 2 cut(s) 993, 1235
Bsh1236I CGCG 3 cut(s) 175, 782, 1071
BshFI GGCC 2 cut(s) 933, 1094
BshNI GGYRCC 2 cut(s) 164, 903
BshTI ACCGGT 1 cut(s) 206
BsiHKAI GWGCWC 1 cut(s) 38
BsiSI CCGG 5 cut(s) 153, 162, 188, 207, 434
BslFI GGGAC 1 cut(s) 659
BslI CCNNNNNNNGG 6 cut(s) 174, 300, 755, 860, 913, 1121
BsmFI GGGAC 1 cut(s) 659
BsnI GGCC 2 cut(s) 933, 1094
Bsp1286I GDGCHC 2 cut(s) 38, 700
Bsp1407I TGTACA 1 cut(s) 1014
Bsp143I GATC 4 cut(s) 308, 740, 1226, 1357
Bsp19I CCATGG 1 cut(s) 998
BspANI GGCC 2 cut(s) 933, 1094
BspCNI CTCAG 1 cut(s) 273
BspFNI CGCG 3 cut(s) 175, 782, 1071
BspLI GGNNCC 6 cut(s) 166, 253, 474, 697, 905, 1113
BspMI ACCTGC 1 cut(s) 1015
BspPI GGATC 2 cut(s) 748, 1234
BspQI GCTCTTC 1 cut(s) 76
BspT107I GGYRCC 2 cut(s) 164, 903
BsrDI GCAATG 1 cut(s) 1071
BsrFI RCCGGY 2 cut(s) 187, 206
BsrGI TGTACA 1 cut(s) 1014
BsrI ACTGG 4 cut(s) 735, 1174, 1286, 1325
BssAI RCCGGY 2 cut(s) 187, 206
BssECI CCNNGG 2 cut(s) 998, 1147
BssMI GATC 4 cut(s) 308, 740, 1226, 1357
BssNI GRCGYC 2 cut(s) 51, 165
BssT1I CCWWGG 2 cut(s) 998, 1147
Bst2UI CCWGG 1 cut(s) 688
Bst4CI ACNGT 9 cut(s) 18, 203, 271, 379, 448, 631, 1014, 1036, 1213
Bst6I CTCTTC 2 cut(s) 76, 97
BstACI GRCGYC 2 cut(s) 51, 165
BstAUI TGTACA 1 cut(s) 1014
BstC8I GCNNGC 2 cut(s) 189, 573
BstDEI CTNAG 3 cut(s) 281, 806, 1269
BstDSI CCRYGG 1 cut(s) 998
BstF5I GGATG 4 cut(s) 38, 354, 990, 1354
BstFNI CGCG 3 cut(s) 175, 782, 1071
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 5 cut(s) 167, 175, 782, 1064, 1071
BstKTI GATC 4 cut(s) 311, 743, 1229, 1360
BstMBI GATC 4 cut(s) 308, 740, 1226, 1357
BstMWI GCNNNNNNNGC 7 cut(s) 59, 75, 184, 467, 605, 1068, 1133
BstNI CCWGG 1 cut(s) 688
BstSCI CCNGG 3 cut(s) 151, 432, 686
BstSFI CTRYAG 1 cut(s) 378
BstUI CGCG 3 cut(s) 175, 782, 1071
BstV1I GCAGC 2 cut(s) 711, 1171
BstV2I GAAGAC 1 cut(s) 132
BstX2I RGATCY 1 cut(s) 740
BstXI CCANNNNNNTGG 1 cut(s) 957
BstYI RGATCY 1 cut(s) 740
BsuI GTATCC 1 cut(s) 996
BsuRI GGCC 2 cut(s) 933, 1094
BtgI CCRYGG 1 cut(s) 998
BtgZI GCGATG 1 cut(s) 1086
BtsCI GGATG 4 cut(s) 38, 354, 990, 1354
BtsIMutI CAGTG 7 cut(s) 375, 627, 742, 1010, 1032, 1218, 1293
BveI ACCTGC 1 cut(s) 1015
Cac8I GCNNGC 2 cut(s) 189, 573
CfoI GCGC 5 cut(s) 167, 175, 782, 1064, 1071
Cfr10I RCCGGY 2 cut(s) 187, 206
Cfr13I GGNCC 2 cut(s) 209, 251
CpoI CGGWCCG 1 cut(s) 209
CseI GACGC 2 cut(s) 59, 597
CsiI ACCWGGT 1 cut(s) 686
Csp6I GTAC 4 cut(s) 204, 684, 904, 1015
CspAI ACCGGT 1 cut(s) 206
CspI CGGWCCG 1 cut(s) 209
CviAII CATG 5 cut(s) 30, 703, 918, 999, 1137
CviQI GTAC 4 cut(s) 204, 684, 904, 1015
DdeI CTNAG 3 cut(s) 281, 806, 1269
DinI GGCGCC 1 cut(s) 166
DpnI GATC 4 cut(s) 310, 742, 1228, 1359
DpnII GATC 4 cut(s) 308, 740, 1226, 1357
DraIII CACNNNGTG 1 cut(s) 20
EaeI YGGCCR 1 cut(s) 1092
Eam1104I CTCTTC 2 cut(s) 76, 97
EarI CTCTTC 2 cut(s) 76, 97
EciI GGCGGA 1 cut(s) 334
Ecl136II GAGCTC 1 cut(s) 36
Eco130I CCWWGG 2 cut(s) 998, 1147
Eco24I GRGCYC 2 cut(s) 38, 700
Eco47I GGWCC 2 cut(s) 209, 251
Eco53kI GAGCTC 1 cut(s) 36
EcoICRI GAGCTC 1 cut(s) 36
EcoRII CCWGG 1 cut(s) 686
EcoT14I CCWWGG 2 cut(s) 998, 1147
EcoT38I GRGCYC 2 cut(s) 38, 700
EgeI GGCGCC 1 cut(s) 166
EheI GGCGCC 1 cut(s) 166
ErhI CCWWGG 2 cut(s) 998, 1147
FaeI CATG 5 cut(s) 33, 706, 921, 1002, 1140
FaqI GGGAC 1 cut(s) 659
FatI CATG 5 cut(s) 29, 702, 917, 998, 1136
FauI CCCGC 1 cut(s) 1246
FauNDI CATATG 2 cut(s) 801, 899
Fnu4HI GCNGC 5 cut(s) 6, 176, 438, 725, 1185
FokI GGATG 4 cut(s) 25, 341, 997, 1361
FriOI GRGCYC 2 cut(s) 38, 700
Fsp4HI GCNGC 5 cut(s) 6, 176, 438, 725, 1185
FspBI CTAG 2 cut(s) 390, 1196
GlaI GCGC 5 cut(s) 166, 174, 781, 1063, 1070
GluI GCNGC 5 cut(s) 6, 176, 438, 725, 1185
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 2 cut(s) 933, 1094
HapII CCGG 5 cut(s) 153, 162, 188, 207, 434
HgaI GACGC 2 cut(s) 59, 597
HhaI GCGC 5 cut(s) 167, 175, 782, 1064, 1071
Hin1I GRCGYC 2 cut(s) 51, 165
Hin1II CATG 5 cut(s) 33, 706, 921, 1002, 1140
Hin6I GCGC 5 cut(s) 165, 173, 780, 1062, 1069
HinP1I GCGC 5 cut(s) 165, 173, 780, 1062, 1069
HindIII AAGCTT 1 cut(s) 1204
HinfI GANTC 4 cut(s) 85, 214, 543, 731
HpaII CCGG 5 cut(s) 153, 162, 188, 207, 434
HphI GGTGA 5 cut(s) 6, 115, 141, 241, 518
Hpy166II GTNNAC 4 cut(s) 444, 627, 960, 1017
Hpy188I TCNGA 5 cut(s) 213, 497, 595, 716, 1297
Hpy188III TCNNGA 4 cut(s) 775, 825, 1341, 1355
Hpy8I GTNNAC 4 cut(s) 444, 627, 960, 1017
Hpy99I CGWCG 3 cut(s) 53, 114, 139
HpyAV CCTTC 2 cut(s) 368, 1257
HpyCH4III ACNGT 9 cut(s) 18, 203, 271, 379, 448, 631, 1014, 1036, 1213
HpyCH4V TGCA 4 cut(s) 527, 571, 1010, 1216
HpyF10VI GCNNNNNNNGC 7 cut(s) 59, 75, 184, 467, 605, 1068, 1133
HpyF3I CTNAG 3 cut(s) 281, 806, 1269
Hsp92I GRCGYC 2 cut(s) 51, 165
Hsp92II CATG 5 cut(s) 33, 706, 921, 1002, 1140
HspAI GCGC 5 cut(s) 165, 173, 780, 1062, 1069
KasI GGCGCC 1 cut(s) 164
KpnI GGTACC 1 cut(s) 907
KroI GCCGGC 1 cut(s) 187
KroNI GCCGGC 1 cut(s) 189
Kzo9I GATC 4 cut(s) 308, 740, 1226, 1357
LguI GCTCTTC 1 cut(s) 76
LmnI GCTCC 7 cut(s) 33, 164, 351, 665, 695, 1111, 1357
Lsp1109I GCAGC 2 cut(s) 711, 1171
LweI GCATC 3 cut(s) 363, 586, 1339
MabI ACCWGGT 1 cut(s) 686
MaeI CTAG 2 cut(s) 390, 1196
MaeIII GTNAC 1 cut(s) 271
MalI GATC 4 cut(s) 310, 742, 1228, 1359
MboI GATC 4 cut(s) 308, 740, 1226, 1357
MboII GAAGA 4 cut(s) 63, 84, 132, 322
MflI RGATCY 1 cut(s) 740
MhlI GDGCHC 2 cut(s) 38, 700
MluCI AATT 4 cut(s) 194, 394, 531, 1098
Mly113I GGCGCC 1 cut(s) 165
MlyI GAGTC 1 cut(s) 223
MmeI TCCRAC 4 cut(s) 242, 694, 736, 1154
MnlI CCTC 9 cut(s) 126, 315, 346, 497, 710, 864, 917, 944, 1330
MroNI GCCGGC 1 cut(s) 187
MseI TTAA 3 cut(s) 366, 945, 1208
MslI CAYNNNNRTG 1 cut(s) 955
MspI CCGG 5 cut(s) 153, 162, 188, 207, 434
MspR9I CCNGG 3 cut(s) 153, 434, 688
MvaI CCWGG 1 cut(s) 688
MvnI CGCG 3 cut(s) 175, 782, 1071
MwoI GCNNNNNNNGC 7 cut(s) 59, 75, 184, 467, 605, 1068, 1133
NaeI GCCGGC 1 cut(s) 189
NarI GGCGCC 1 cut(s) 165
NciI CCSGG 2 cut(s) 153, 434
NcoI CCATGG 1 cut(s) 998
NdeI CATATG 2 cut(s) 801, 899
NdeII GATC 4 cut(s) 308, 740, 1226, 1357
NgoMIV GCCGGC 1 cut(s) 187
NlaIII CATG 5 cut(s) 33, 706, 921, 1002, 1140
NlaIV GGNNCC 6 cut(s) 166, 253, 474, 697, 905, 1113
PciSI GCTCTTC 1 cut(s) 76
PcsI WCGNNNNNNNCGW 1 cut(s) 115
PdiI GCCGGC 1 cut(s) 189
PfeI GAWTC 3 cut(s) 85, 543, 731
PflMI CCANNNNNTGG 1 cut(s) 755
PinAI ACCGGT 1 cut(s) 206
PkrI GCNGC 5 cut(s) 7, 177, 439, 726, 1186
PleI GAGTC 1 cut(s) 222
PluTI GGCGCC 1 cut(s) 168
PpsI GAGTC 1 cut(s) 222
Psp124BI GAGCTC 1 cut(s) 38
Psp6I CCWGG 1 cut(s) 686
PspGI CCWGG 1 cut(s) 686
PspN4I GGNNCC 6 cut(s) 166, 253, 474, 697, 905, 1113
PspPI GGNCC 2 cut(s) 209, 251
PsuI RGATCY 1 cut(s) 740
RsaI GTAC 4 cut(s) 205, 685, 905, 1016
RsaNI GTAC 4 cut(s) 204, 684, 904, 1015
RseI CAYNNNNRTG 1 cut(s) 955
Rsr2I CGGWCCG 1 cut(s) 209
RsrII CGGWCCG 1 cut(s) 209
SacI GAGCTC 1 cut(s) 38
SapI GCTCTTC 1 cut(s) 76
SaqAI TTAA 3 cut(s) 366, 945, 1208
SatI GCNGC 5 cut(s) 6, 176, 438, 725, 1185
Sau3AI GATC 4 cut(s) 308, 740, 1226, 1357
Sau96I GGNCC 2 cut(s) 209, 251
SchI GAGTC 1 cut(s) 223
ScrFI CCNGG 3 cut(s) 153, 434, 688
SduI GDGCHC 2 cut(s) 38, 700
SexAI ACCWGGT 1 cut(s) 686
SfaNI GCATC 3 cut(s) 363, 586, 1339
SfcI CTRYAG 1 cut(s) 378
SfoI GGCGCC 1 cut(s) 166
SinI GGWCC 2 cut(s) 209, 251
SmiMI CAYNNNNRTG 1 cut(s) 955
SmlI CTYRAG 1 cut(s) 775
SmoI CTYRAG 1 cut(s) 775
Sse9I AATT 4 cut(s) 194, 394, 531, 1098
SspDI GGCGCC 1 cut(s) 164
SspMI CTAG 2 cut(s) 390, 1196
SstI GAGCTC 1 cut(s) 38
StyD4I CCNGG 3 cut(s) 151, 432, 686
StyI CCWWGG 2 cut(s) 998, 1147
TaaI ACNGT 9 cut(s) 18, 203, 271, 379, 448, 631, 1014, 1036, 1213
TaqI TCGA 2 cut(s) 83, 401
TasI AATT 4 cut(s) 194, 394, 531, 1098
TatI WGTACW 1 cut(s) 1014
TauI GCSGC 2 cut(s) 178, 440
TfiI GAWTC 3 cut(s) 85, 543, 731
Tru1I TTAA 3 cut(s) 366, 945, 1208
Tru9I TTAA 3 cut(s) 366, 945, 1208
TscAI CASTG 7 cut(s) 382, 634, 742, 1017, 1039, 1218, 1293
TseI GCWGC 3 cut(s) 5, 724, 1184
TspDTI ATGAA 3 cut(s) 788, 1272, 1277
TspRI CASTG 7 cut(s) 382, 634, 742, 1017, 1039, 1218, 1293
Van91I CCANNNNNTGG 1 cut(s) 755
VpaK11BI GGWCC 2 cut(s) 209, 251
XcmI CCANNNNNNNNNTGG 1 cut(s) 17
XspI CTAG 2 cut(s) 390, 1196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.