Rh4BG400600

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0016838
Physical Location & Seq
Forward (+)
55109085 .. 55111876
2792 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG400600.1

Sequence Viewer

Length: 1275 bp
ATGGAGCTCATCCACCTACGACGCCACCACCACCAAATGCTCTTCCAGCTCATCGAATCTCTCTTCGTTTTATACAAAACGACCTCGTTTCACCGCTGTTCGTCTTCTAAATCACCCGGTAGCTCCGGCGCCAAAGCGCGGCTGCGGCTGAAAAAGCCGGCGAAATTGGACGGTACCGGTCCGAGTCCAACCGCATTAGTCAAAACAGCAAAGGGAAATGGACCCAGAAGTGAAAAGACGGTAACAGCCCTGAGCTTCAAGTCTTTGTCTGGGAAGCGATCTCTATGGCGGAGGATATTCTTCGCCTCCAAGAAAGTTAGGAGCATCCTTCTGCTTAATGTAATCACTGTAGTCTATGCTAGTAATTTTTCGATTGTGAAAGAAGTGGAAGCTGTGATAGACCCAGCGGCGTTTACTGTAGTGCGGTTTGCTATGTCGGCGGTTCCATTTATCCCATTTGTTTTCCGAGCAAGAGGTGATGTTGATACTCGTAATGCAGGAATTGAGTTGGGATTCTGGGTTAGTTTAGGGTATCTTATGCAGGCATTAGGACTGCTAACATCAGATGCTGGTCGTGCGTCATTTCTTTCTATGTTCACTGTGATTATCGTTCCCTTGCTTGATGGAATGTTAGGAGCAATAGTCCCTGCTCGTACCTGGTTTGGAGCCCTCATGTCTATTGTAGGAGTTGGGCTGCTGGAATCCAGTGGATCTCCCCCAAATGTTGGAGATTTGCTGAACTTCTTGAGCGCGGTGTTTTTTGGGGTTCATATGCTTAGAACAGAGCATATATCAAGAAACACAAAAAAAGAAAACTTCTTGCCTCTGCTTGGATATGAGATATGCGTTGTTGCTTTCTTATCAACCATATGGTACCTCATTGGGGCATGGTCTGGTGGTGGCCTCCACAACTTTAACCCACCATTGTGGACATGGGAGATGTTCTGGGATTGGATGGTTGTGTTTCCATGGATACCTGCACTGTACACAGGCATTTTCTCCACTGTTTTATGCTTATGGGTAGAGATGGGCGCAATGCGCGATGTATCAGCCACAGAAACAGCCATAATTTATGGTTTGGAGCCTGTCTGGGGTGCTGGTGGAAGCTTAACAGTGCAGATATTTGGATCAACATCTCCCACAGAGCCTTCAAAAGATGAAATAAAAGCTAAGAAACTCACTTTCCCAGTGGTTTCAGATAAACACAATGGCTTATCTGCCTCGCCAGTTATAGTCAGTTCCAGAAAGGATACTCCTGATCTGCGGAGTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

424

Amino Acids

46.61

Weight (kDa)

9.78

Isoelectric Point (pI)

39.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 108 - 233 8.2e-08 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016838)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g32690
malus_domestica MD16G1026300.v1.1
prunus_persica Prupe.1G327200_v2.0.a1
pyrus_communis pycom16g02220
rosa_chinensis RchiOBHm_Chr4g0441611
rosa_laevigata RLG00000006081
rosa_multiflora Rmu_sc0000353.1_g000024
rosa_roxburghii Rroxscaffold_5G00382440
rosa_rugosa Rorug04G0334900
rosa_samantha Rh4AG386400 Rh4BG400600 Rh4CG415600 Rh4DG393300
rosa_wichuraiana Rw4G033380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 985
Acc65I GGTACC 2 cut(s) 173, 873
AccB1I GGYRCC 3 cut(s) 128, 173, 873
AccB7I CCANNNNNTGG 1 cut(s) 725
AccII CGCG 3 cut(s) 139, 752, 1041
AclWI GGATC 2 cut(s) 718, 1135
AcyI GRCGYC 2 cut(s) 22, 129
AfaI GTAC 4 cut(s) 175, 655, 875, 986
AfiI CCNNNNNNNGG 5 cut(s) 138, 725, 830, 883, 1091
AgeI ACCGGT 1 cut(s) 176
AgsI TTSAA 2 cut(s) 259, 1152
AjnI CCWGG 1 cut(s) 656
AleI CACNNNNGTG 1 cut(s) 925
AluBI AGCT 7 cut(s) 7, 49, 123, 255, 392, 1107, 1169
AluI AGCT 7 cut(s) 7, 49, 123, 255, 392, 1107, 1169
Alw21I GWGCWC 1 cut(s) 9
AlwI GGATC 2 cut(s) 718, 1135
AlwNI CAGNNNCTG 1 cut(s) 569
AoxI GGCC 1 cut(s) 901
ApeKI GCWGC 2 cut(s) 142, 694
AsiGI ACCGGT 1 cut(s) 176
Asp718I GGTACC 2 cut(s) 173, 873
AspLEI GCGC 5 cut(s) 131, 139, 752, 1034, 1041
AspS9I GGNCC 2 cut(s) 179, 221
AsuC2I CCSGG 1 cut(s) 117
AsuHPI GGTGA 3 cut(s) 83, 105, 488
AvaII GGWCC 2 cut(s) 179, 221
BanI GGYRCC 3 cut(s) 128, 173, 873
BanII GRGCYC 2 cut(s) 9, 670
BbsI GAAGAC 1 cut(s) 96
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 2 cut(s) 129, 681
BccI CCATC 3 cut(s) 617, 949, 1021
BciT130I CCWGG 1 cut(s) 658
BciVI GTATCC 2 cut(s) 966, 1243
BcnI CCSGG 1 cut(s) 117
BfaI CTAG 1 cut(s) 360
BfmI CTRYAG 2 cut(s) 348, 417
BfoI RGCGCY 1 cut(s) 132
BfuAI ACCTGC 1 cut(s) 985
BfuI GTATCC 2 cut(s) 966, 1243
BisI GCNGC 5 cut(s) 140, 143, 146, 408, 695
BlsI GCNGC 5 cut(s) 141, 144, 147, 409, 696
Bme1390I CCNGG 2 cut(s) 117, 658
Bme18I GGWCC 2 cut(s) 179, 221
BmgT120I GGNCC 2 cut(s) 179, 221
BmiI GGNNCC 7 cut(s) 130, 175, 223, 444, 667, 875, 1083
BmrFI CCNGG 2 cut(s) 117, 658
BmrI ACTGGG 1 cut(s) 1181
BmsI GCATC 2 cut(s) 333, 556
BmuI ACTGGG 1 cut(s) 1181
BpiI GAAGAC 1 cut(s) 96
Bpu10I CCTNAGC 1 cut(s) 251
BpuEI CTTGAG 1 cut(s) 766
BpuMI CCSGG 1 cut(s) 117
BsaBI GATNNNNATC 1 cut(s) 1132
BsaHI GRCGYC 2 cut(s) 22, 129
BsaJI CCNNGG 1 cut(s) 968
BsaWI WCCGGW 1 cut(s) 176
BsaXI ACNNNNNCTCC 2 cut(s) 627, 657
Bsc4I CCNNNNNNNGG 5 cut(s) 138, 725, 830, 883, 1091
Bse118I RCCGGY 2 cut(s) 157, 176
Bse1I ACTGG 3 cut(s) 705, 1187, 1226
Bse3DI GCAATG 1 cut(s) 1041
Bse8I GATNNNNATC 1 cut(s) 1132
BseBI CCWGG 1 cut(s) 658
BseDI CCNNGG 1 cut(s) 968
BseGI GGATG 3 cut(s) 9, 324, 960
BseJI GATNNNNATC 1 cut(s) 1132
BseLI CCNNNNNNNGG 5 cut(s) 138, 725, 830, 883, 1091
BseMI GCAATG 1 cut(s) 1041
BseMII CTCAG 1 cut(s) 242
BseNI ACTGG 3 cut(s) 705, 1187, 1226
BseXI GCAGC 2 cut(s) 129, 681
BseYI CCCAGC 1 cut(s) 403
BsgI GTGCAG 2 cut(s) 963, 1136
Bsh1236I CGCG 3 cut(s) 139, 752, 1041
BshFI GGCC 1 cut(s) 903
BshNI GGYRCC 3 cut(s) 128, 173, 873
BshTI ACCGGT 1 cut(s) 176
BsiHKAI GWGCWC 1 cut(s) 9
BsiSI CCGG 4 cut(s) 117, 126, 158, 177
BslFI GGGAC 1 cut(s) 629
BslI CCNNNNNNNGG 5 cut(s) 138, 725, 830, 883, 1091
BsmFI GGGAC 1 cut(s) 629
BsnI GGCC 1 cut(s) 903
Bsp1286I GDGCHC 2 cut(s) 9, 670
Bsp1407I TGTACA 1 cut(s) 984
Bsp143I GATC 4 cut(s) 278, 710, 1127, 1258
Bsp19I CCATGG 1 cut(s) 968
BspANI GGCC 1 cut(s) 903
BspCNI CTCAG 1 cut(s) 243
BspFNI CGCG 3 cut(s) 139, 752, 1041
BspLI GGNNCC 7 cut(s) 130, 175, 223, 444, 667, 875, 1083
BspMI ACCTGC 1 cut(s) 985
BspPI GGATC 2 cut(s) 718, 1135
BspQI GCTCTTC 1 cut(s) 47
BspT107I GGYRCC 3 cut(s) 128, 173, 873
BsrDI GCAATG 1 cut(s) 1041
BsrFI RCCGGY 2 cut(s) 157, 176
BsrGI TGTACA 1 cut(s) 984
BsrI ACTGG 3 cut(s) 705, 1187, 1226
BssAI RCCGGY 2 cut(s) 157, 176
BssECI CCNNGG 1 cut(s) 968
BssMI GATC 4 cut(s) 278, 710, 1127, 1258
BssNI GRCGYC 2 cut(s) 22, 129
BssT1I CCWWGG 1 cut(s) 968
Bst2UI CCWGG 1 cut(s) 658
Bst4CI ACNGT 8 cut(s) 173, 241, 349, 418, 601, 984, 1006, 1114
Bst6I CTCTTC 2 cut(s) 47, 68
BstACI GRCGYC 2 cut(s) 22, 129
BstAUI TGTACA 1 cut(s) 984
BstC8I GCNNGC 2 cut(s) 159, 543
BstDEI CTNAG 3 cut(s) 251, 776, 1170
BstDSI CCRYGG 1 cut(s) 968
BstF5I GGATG 3 cut(s) 9, 324, 960
BstFNI CGCG 3 cut(s) 139, 752, 1041
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 5 cut(s) 131, 139, 752, 1034, 1041
BstKTI GATC 4 cut(s) 281, 713, 1130, 1261
BstMBI GATC 4 cut(s) 278, 710, 1127, 1258
BstMWI GCNNNNNNNGC 6 cut(s) 46, 145, 154, 437, 575, 1038
BstNI CCWGG 1 cut(s) 658
BstSCI CCNGG 2 cut(s) 115, 656
BstSFI CTRYAG 2 cut(s) 348, 417
BstUI CGCG 3 cut(s) 139, 752, 1041
BstV1I GCAGC 2 cut(s) 129, 681
BstV2I GAAGAC 1 cut(s) 96
BstX2I RGATCY 1 cut(s) 710
BstXI CCANNNNNNTGG 1 cut(s) 927
BstYI RGATCY 1 cut(s) 710
BsuI GTATCC 2 cut(s) 966, 1243
BsuRI GGCC 1 cut(s) 903
BtgI CCRYGG 1 cut(s) 968
BtgZI GCGATG 1 cut(s) 1056
BtsCI GGATG 3 cut(s) 9, 324, 960
BtsIMutI CAGTG 7 cut(s) 345, 597, 712, 980, 1002, 1119, 1194
BveI ACCTGC 1 cut(s) 985
Cac8I GCNNGC 2 cut(s) 159, 543
CaiI CAGNNNCTG 1 cut(s) 569
CfoI GCGC 5 cut(s) 131, 139, 752, 1034, 1041
Cfr10I RCCGGY 2 cut(s) 157, 176
Cfr13I GGNCC 2 cut(s) 179, 221
CpoI CGGWCCG 1 cut(s) 179
CseI GACGC 2 cut(s) 30, 567
CsiI ACCWGGT 1 cut(s) 656
Csp6I GTAC 4 cut(s) 174, 654, 874, 985
CspAI ACCGGT 1 cut(s) 176
CspI CGGWCCG 1 cut(s) 179
CviAII CATG 4 cut(s) 673, 888, 933, 969
CviQI GTAC 4 cut(s) 174, 654, 874, 985
DdeI CTNAG 3 cut(s) 251, 776, 1170
DinI GGCGCC 1 cut(s) 130
DpnI GATC 4 cut(s) 280, 712, 1129, 1260
DpnII GATC 4 cut(s) 278, 710, 1127, 1258
Eam1104I CTCTTC 2 cut(s) 47, 68
EarI CTCTTC 2 cut(s) 47, 68
EciI GGCGGA 1 cut(s) 304
Ecl136II GAGCTC 1 cut(s) 7
Eco130I CCWWGG 1 cut(s) 968
Eco24I GRGCYC 2 cut(s) 9, 670
Eco47I GGWCC 2 cut(s) 179, 221
Eco53kI GAGCTC 1 cut(s) 7
EcoICRI GAGCTC 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 656
EcoT14I CCWWGG 1 cut(s) 968
EcoT38I GRGCYC 2 cut(s) 9, 670
EgeI GGCGCC 1 cut(s) 130
EheI GGCGCC 1 cut(s) 130
ErhI CCWWGG 1 cut(s) 968
FaeI CATG 4 cut(s) 676, 891, 936, 972
FaqI GGGAC 1 cut(s) 629
FatI CATG 4 cut(s) 672, 887, 932, 968
FauNDI CATATG 2 cut(s) 771, 869
Fnu4HI GCNGC 5 cut(s) 140, 143, 146, 408, 695
FokI GGATG 2 cut(s) 311, 967
FriOI GRGCYC 2 cut(s) 9, 670
Fsp4HI GCNGC 5 cut(s) 140, 143, 146, 408, 695
FspBI CTAG 1 cut(s) 360
GlaI GCGC 5 cut(s) 130, 138, 751, 1033, 1040
GluI GCNGC 5 cut(s) 140, 143, 146, 408, 695
GsaI CCCAGC 1 cut(s) 407
HaeII RGCGCY 1 cut(s) 132
HaeIII GGCC 1 cut(s) 903
HapII CCGG 4 cut(s) 117, 126, 158, 177
HgaI GACGC 2 cut(s) 30, 567
HhaI GCGC 5 cut(s) 131, 139, 752, 1034, 1041
Hin1I GRCGYC 2 cut(s) 22, 129
Hin1II CATG 4 cut(s) 676, 891, 936, 972
Hin6I GCGC 5 cut(s) 129, 137, 750, 1032, 1039
HinP1I GCGC 5 cut(s) 129, 137, 750, 1032, 1039
HindIII AAGCTT 1 cut(s) 1105
HinfI GANTC 4 cut(s) 56, 184, 513, 701
HpaII CCGG 4 cut(s) 117, 126, 158, 177
HphI GGTGA 3 cut(s) 83, 105, 488
Hpy166II GTNNAC 4 cut(s) 414, 597, 930, 987
Hpy188I TCNGA 4 cut(s) 183, 467, 565, 1198
Hpy188III TCNNGA 4 cut(s) 745, 795, 1242, 1256
Hpy8I GTNNAC 4 cut(s) 414, 597, 930, 987
Hpy99I CGWCG 1 cut(s) 24
HpyAV CCTTC 2 cut(s) 338, 1158
HpyCH4III ACNGT 8 cut(s) 173, 241, 349, 418, 601, 984, 1006, 1114
HpyCH4V TGCA 4 cut(s) 497, 541, 980, 1117
HpyF10VI GCNNNNNNNGC 6 cut(s) 46, 145, 154, 437, 575, 1038
HpyF3I CTNAG 3 cut(s) 251, 776, 1170
Hsp92I GRCGYC 2 cut(s) 22, 129
Hsp92II CATG 4 cut(s) 676, 891, 936, 972
HspAI GCGC 5 cut(s) 129, 137, 750, 1032, 1039
KasI GGCGCC 1 cut(s) 128
KpnI GGTACC 2 cut(s) 177, 877
KroI GCCGGC 1 cut(s) 157
KroNI GCCGGC 1 cut(s) 159
Kzo9I GATC 4 cut(s) 278, 710, 1127, 1258
LguI GCTCTTC 1 cut(s) 47
LmnI GCTCC 6 cut(s) 4, 128, 321, 635, 665, 1081
Lsp1109I GCAGC 2 cut(s) 129, 681
LweI GCATC 2 cut(s) 333, 556
MabI ACCWGGT 1 cut(s) 656
MaeI CTAG 1 cut(s) 360
MaeIII GTNAC 1 cut(s) 241
MalI GATC 4 cut(s) 280, 712, 1129, 1260
MboI GATC 4 cut(s) 278, 710, 1127, 1258
MboII GAAGA 4 cut(s) 34, 55, 96, 292
MflI RGATCY 1 cut(s) 710
MhlI GDGCHC 2 cut(s) 9, 670
MluCI AATT 4 cut(s) 164, 364, 501, 1068
Mly113I GGCGCC 1 cut(s) 129
MlyI GAGTC 1 cut(s) 193
MmeI TCCRAC 2 cut(s) 212, 706
MnlI CCTC 9 cut(s) 94, 285, 316, 467, 680, 834, 887, 914, 1231
MroNI GCCGGC 1 cut(s) 157
MseI TTAA 3 cut(s) 336, 915, 1109
MslI CAYNNNNRTG 1 cut(s) 925
MspA1I CMGCKG 2 cut(s) 96, 407
MspI CCGG 4 cut(s) 117, 126, 158, 177
MspR9I CCNGG 2 cut(s) 117, 658
MvaI CCWGG 1 cut(s) 658
MvnI CGCG 3 cut(s) 139, 752, 1041
MwoI GCNNNNNNNGC 6 cut(s) 46, 145, 154, 437, 575, 1038
NaeI GCCGGC 1 cut(s) 159
NarI GGCGCC 1 cut(s) 129
NciI CCSGG 1 cut(s) 117
NcoI CCATGG 1 cut(s) 968
NdeI CATATG 2 cut(s) 771, 869
NdeII GATC 4 cut(s) 278, 710, 1127, 1258
NgoMIV GCCGGC 1 cut(s) 157
NlaIII CATG 4 cut(s) 676, 891, 936, 972
NlaIV GGNNCC 7 cut(s) 130, 175, 223, 444, 667, 875, 1083
OliI CACNNNNGTG 1 cut(s) 925
PciSI GCTCTTC 1 cut(s) 47
PdiI GCCGGC 1 cut(s) 159
PfeI GAWTC 3 cut(s) 56, 513, 701
PflMI CCANNNNNTGG 1 cut(s) 725
PinAI ACCGGT 1 cut(s) 176
PkrI GCNGC 5 cut(s) 141, 144, 147, 409, 696
PleI GAGTC 1 cut(s) 192
PluTI GGCGCC 1 cut(s) 132
PpsI GAGTC 1 cut(s) 192
Psp124BI GAGCTC 1 cut(s) 9
Psp6I CCWGG 1 cut(s) 656
PspFI CCCAGC 1 cut(s) 403
PspGI CCWGG 1 cut(s) 656
PspN4I GGNNCC 7 cut(s) 130, 175, 223, 444, 667, 875, 1083
PspPI GGNCC 2 cut(s) 179, 221
PstNI CAGNNNCTG 1 cut(s) 569
PsuI RGATCY 1 cut(s) 710
RsaI GTAC 4 cut(s) 175, 655, 875, 986
RsaNI GTAC 4 cut(s) 174, 654, 874, 985
RseI CAYNNNNRTG 1 cut(s) 925
Rsr2I CGGWCCG 1 cut(s) 179
RsrII CGGWCCG 1 cut(s) 179
SacI GAGCTC 1 cut(s) 9
SapI GCTCTTC 1 cut(s) 47
SaqAI TTAA 3 cut(s) 336, 915, 1109
SatI GCNGC 5 cut(s) 140, 143, 146, 408, 695
Sau3AI GATC 4 cut(s) 278, 710, 1127, 1258
Sau96I GGNCC 2 cut(s) 179, 221
SchI GAGTC 1 cut(s) 193
ScrFI CCNGG 2 cut(s) 117, 658
SduI GDGCHC 2 cut(s) 9, 670
SexAI ACCWGGT 1 cut(s) 656
SfaNI GCATC 2 cut(s) 333, 556
SfcI CTRYAG 2 cut(s) 348, 417
SfoI GGCGCC 1 cut(s) 130
SinI GGWCC 2 cut(s) 179, 221
SmiMI CAYNNNNRTG 1 cut(s) 925
SmlI CTYRAG 1 cut(s) 745
SmoI CTYRAG 1 cut(s) 745
Sse9I AATT 4 cut(s) 164, 364, 501, 1068
SspDI GGCGCC 1 cut(s) 128
SspMI CTAG 1 cut(s) 360
SstI GAGCTC 1 cut(s) 9
StyD4I CCNGG 2 cut(s) 115, 656
StyI CCWWGG 1 cut(s) 968
TaaI ACNGT 8 cut(s) 173, 241, 349, 418, 601, 984, 1006, 1114
TaqI TCGA 2 cut(s) 54, 371
TasI AATT 4 cut(s) 164, 364, 501, 1068
TatI WGTACW 1 cut(s) 984
TauI GCSGC 3 cut(s) 142, 148, 410
TfiI GAWTC 3 cut(s) 56, 513, 701
Tru1I TTAA 3 cut(s) 336, 915, 1109
Tru9I TTAA 3 cut(s) 336, 915, 1109
TscAI CASTG 7 cut(s) 352, 604, 712, 987, 1009, 1119, 1194
TseI GCWGC 2 cut(s) 142, 694
TspDTI ATGAA 2 cut(s) 758, 1173
TspRI CASTG 7 cut(s) 352, 604, 712, 987, 1009, 1119, 1194
Van91I CCANNNNNTGG 1 cut(s) 725
VpaK11BI GGWCC 2 cut(s) 179, 221
XcmI CCANNNNNNNNNTGG 1 cut(s) 930
XspI CTAG 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.