RLG00000006081

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0016838
Physical Location & Seq
Reverse (-)
4174556 .. 4177384
2829 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006081

Sequence Viewer

Length: 1374 bp
ATGGCTGCCCAATCACCGTGCGGGTGGTCATGGAGCTCATCCACCTACGACGCCACCACCGCCAAATGCTCTTCCAGCTCATCGAATCTCTCTTCGTTTTATACAAAACGACCTCGTTTCAACGCCTGTTCGTCTTCTAAATCACCCGGTAGCTCCGGCGCCAAAGCGCGGCTGAAAAAGCCGGCGAAATTGGACAGTACCGGTCCGAGTCCAACCGCATTAGTGAAAACCGCCAAGGGAAATGGACCCAGAAGTGAAAAGACGGTAACAGCCCTGAGCTTCAAGTCCTTGTCTGGGAAGCGATCTCGATCTCTATGGCGGAGGATATTCTTCGCCTCCAAGAAAGTTAGGAGCATCCTTCTGCTTAATGTAATCACTGTAGTCTATGCTAGTAATTTTTCGATTGTGAAAGAAGTGGAAGCTGTGATAGACCCAGCGGCGTTTACTGTTGTGCGGTTTGCTATGTCGGCGGTTCCATTTATCCCATTTGTTTTCCGAGCAAGAGGTGATGTTGATACTCGTAATGCAGGAATTGAGTTGGGATTCTGGGTTAGTTTAGGGTATCTTATGCAGGCATTAGGACTGCTAACATCAGATGCTGGTCGTGCGTCATTTCTTTCTATGTTCACTGTGATTATCGTTCCCTTGCTTGATGGAATGTTAGGAGCAATAGTCCCTGCTCGTACCTGGTTTGGAGCCCTCATGTCTATTGTAGGAGTTGGGCTGCTGGAATCCAGTGGATCTCCCCCAAATGTTGGAGATTTGCTGAACTTCTTGAGCGCGGTGTTTTTTGGGGTTCATATGCTTAGAACAGAGCATATATCAAGAAACACAAAAAAAGAAAACTTTTTGCCTCTGCTTGGATATGAGATATGGGTTGTTGCTTTCTTATCAACCATATGGTACCTCATTGGGGCATGGTCTGGTGGTGGCCTCCACGACTTTAACCCATCATTGTGGACATGGGAGATGTTCTGGGAGTGGATGCTTGTGTTTCCATGGATACCTGCACTGTACACAGGCATTTTCTCCACTGTTTTATGCTTATGGGTAGAGATGGGTGCAATGCGCGATGTATCGGCCACAGAAACGGCCATAATTTATGGTTTGGAGCCTGTCTGGGGTGCTGGTTTCGCATGGTTTCTCCTTGGCGAAAGATGGGGCTCTACTGGTTGGATTGGTGCTGCTCTTGTGCTAGGTGGAAGCTTAACAGTGCAGATATTTGGATCAACATCTCCGACAGAGCCTTCAAAAGATGAAATGAAAGCTAAGAAACTCACTTTCCCAGCGGTTTCGGATAAGCACAATGGCTTATCTGCCTCGCCAGTTATAGTCAGTTCCAGAAAGGATGCTCCTGATCTGCGGAGTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

458

Amino Acids

49.66

Weight (kDa)

9.69

Isoelectric Point (pI)

43.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 119 - 242 9.4e-08 EamA-like transporter family
EamA PF00892 253 - 403 1.9e-12 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016838)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g32690
malus_domestica MD16G1026300.v1.1
prunus_persica Prupe.1G327200_v2.0.a1
pyrus_communis pycom16g02220
rosa_chinensis RchiOBHm_Chr4g0441611
rosa_laevigata RLG00000006081
rosa_multiflora Rmu_sc0000353.1_g000024
rosa_roxburghii Rroxscaffold_5G00382440
rosa_rugosa Rorug04G0334900
rosa_samantha Rh4AG386400 Rh4BG400600 Rh4CG415600 Rh4DG393300
rosa_wichuraiana Rw4G033380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1015
Acc65I GGTACC 1 cut(s) 903
AccB1I GGYRCC 2 cut(s) 158, 903
AccB7I CCANNNNNTGG 1 cut(s) 755
AccII CGCG 3 cut(s) 169, 782, 1071
AclWI GGATC 2 cut(s) 748, 1234
AcoI YGGCCR 2 cut(s) 1080, 1092
AcyI GRCGYC 2 cut(s) 51, 159
AfaI GTAC 4 cut(s) 199, 685, 905, 1016
AfiI CCNNNNNNNGG 6 cut(s) 168, 294, 755, 860, 913, 1121
AgeI ACCGGT 1 cut(s) 200
AgsI TTSAA 3 cut(s) 121, 283, 1251
AjnI CCWGG 1 cut(s) 686
AluBI AGCT 7 cut(s) 36, 78, 153, 279, 422, 1206, 1268
AluI AGCT 7 cut(s) 36, 78, 153, 279, 422, 1206, 1268
Alw21I GWGCWC 1 cut(s) 38
AlwI GGATC 2 cut(s) 748, 1234
AlwNI CAGNNNCTG 1 cut(s) 599
AoxI GGCC 3 cut(s) 931, 1080, 1092
ApeKI GCWGC 3 cut(s) 5, 724, 1184
AsiGI ACCGGT 1 cut(s) 200
Asp718I GGTACC 1 cut(s) 903
AspLEI GCGC 4 cut(s) 161, 169, 782, 1071
AspS9I GGNCC 2 cut(s) 203, 245
AsuC2I CCSGG 1 cut(s) 147
AsuHPI GGTGA 3 cut(s) 6, 135, 518
AvaII GGWCC 2 cut(s) 203, 245
BanI GGYRCC 2 cut(s) 158, 903
BanII GRGCYC 3 cut(s) 38, 700, 1166
BbsI GAAGAC 1 cut(s) 126
Bbv12I GWGCWC 1 cut(s) 38
BbvI GCAGC 2 cut(s) 711, 1171
BccI CCATC 4 cut(s) 647, 958, 1051, 1152
BceAI ACGGC 1 cut(s) 1107
BciT130I CCWGG 1 cut(s) 688
BciVI GTATCC 1 cut(s) 996
BcnI CCSGG 1 cut(s) 147
BfaI CTAG 2 cut(s) 390, 1196
BfmI CTRYAG 1 cut(s) 378
BfoI RGCGCY 1 cut(s) 162
BfuAI ACCTGC 1 cut(s) 1015
BfuI GTATCC 1 cut(s) 996
BisI GCNGC 5 cut(s) 6, 170, 438, 725, 1185
BlsI GCNGC 5 cut(s) 7, 171, 439, 726, 1186
Bme1390I CCNGG 2 cut(s) 147, 688
Bme18I GGWCC 2 cut(s) 203, 245
BmgT120I GGNCC 2 cut(s) 203, 245
BmiI GGNNCC 6 cut(s) 160, 247, 474, 697, 905, 1113
BmrFI CCNGG 2 cut(s) 147, 688
BmsI GCATC 4 cut(s) 363, 586, 975, 1339
BpiI GAAGAC 1 cut(s) 126
Bpu10I CCTNAGC 1 cut(s) 275
BpuEI CTTGAG 1 cut(s) 796
BpuMI CCSGG 1 cut(s) 147
BsaBI GATNNNNATC 2 cut(s) 307, 1231
BsaHI GRCGYC 2 cut(s) 51, 159
BsaJI CCNNGG 3 cut(s) 234, 998, 1147
BsaWI WCCGGW 1 cut(s) 200
BsaXI ACNNNNNCTCC 2 cut(s) 657, 687
Bsc4I CCNNNNNNNGG 6 cut(s) 168, 294, 755, 860, 913, 1121
Bse118I RCCGGY 2 cut(s) 181, 200
Bse1I ACTGG 3 cut(s) 735, 1174, 1325
Bse3DI GCAATG 1 cut(s) 1071
Bse8I GATNNNNATC 2 cut(s) 307, 1231
BseBI CCWGG 1 cut(s) 688
BseDI CCNNGG 3 cut(s) 234, 998, 1147
BseGI GGATG 4 cut(s) 38, 354, 990, 1354
BseJI GATNNNNATC 2 cut(s) 307, 1231
BseLI CCNNNNNNNGG 6 cut(s) 168, 294, 755, 860, 913, 1121
BseMI GCAATG 1 cut(s) 1071
BseMII CTCAG 1 cut(s) 266
BseNI ACTGG 3 cut(s) 735, 1174, 1325
BseXI GCAGC 2 cut(s) 711, 1171
BseYI CCCAGC 2 cut(s) 433, 1285
BsgI GTGCAG 2 cut(s) 993, 1235
Bsh1236I CGCG 3 cut(s) 169, 782, 1071
BshFI GGCC 3 cut(s) 933, 1082, 1094
BshNI GGYRCC 2 cut(s) 158, 903
BshTI ACCGGT 1 cut(s) 200
BsiHKAI GWGCWC 1 cut(s) 38
BsiSI CCGG 4 cut(s) 147, 156, 182, 201
BslFI GGGAC 1 cut(s) 659
BslI CCNNNNNNNGG 6 cut(s) 168, 294, 755, 860, 913, 1121
BsmFI GGGAC 1 cut(s) 659
BsnI GGCC 3 cut(s) 933, 1082, 1094
Bsp1286I GDGCHC 3 cut(s) 38, 700, 1166
Bsp1407I TGTACA 1 cut(s) 1014
Bsp143I GATC 5 cut(s) 302, 308, 740, 1226, 1357
Bsp19I CCATGG 1 cut(s) 998
BspANI GGCC 3 cut(s) 933, 1082, 1094
BspCNI CTCAG 1 cut(s) 267
BspFNI CGCG 3 cut(s) 169, 782, 1071
BspLI GGNNCC 6 cut(s) 160, 247, 474, 697, 905, 1113
BspMI ACCTGC 1 cut(s) 1015
BspPI GGATC 2 cut(s) 748, 1234
BspQI GCTCTTC 1 cut(s) 76
BspT107I GGYRCC 2 cut(s) 158, 903
BsrDI GCAATG 1 cut(s) 1071
BsrFI RCCGGY 2 cut(s) 181, 200
BsrGI TGTACA 1 cut(s) 1014
BsrI ACTGG 3 cut(s) 735, 1174, 1325
BssAI RCCGGY 2 cut(s) 181, 200
BssECI CCNNGG 3 cut(s) 234, 998, 1147
BssMI GATC 5 cut(s) 302, 308, 740, 1226, 1357
BssNI GRCGYC 2 cut(s) 51, 159
BssT1I CCWWGG 3 cut(s) 234, 998, 1147
Bst2UI CCWGG 1 cut(s) 688
Bst4CI ACNGT 9 cut(s) 18, 197, 265, 379, 448, 631, 1014, 1036, 1213
Bst6I CTCTTC 2 cut(s) 76, 97
BstACI GRCGYC 2 cut(s) 51, 159
BstAUI TGTACA 1 cut(s) 1014
BstC8I GCNNGC 2 cut(s) 183, 573
BstDEI CTNAG 3 cut(s) 275, 806, 1269
BstDSI CCRYGG 1 cut(s) 998
BstF5I GGATG 4 cut(s) 38, 354, 990, 1354
BstFNI CGCG 3 cut(s) 169, 782, 1071
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 4 cut(s) 161, 169, 782, 1071
BstKTI GATC 5 cut(s) 305, 311, 743, 1229, 1360
BstMBI GATC 5 cut(s) 302, 308, 740, 1226, 1357
BstMWI GCNNNNNNNGC 6 cut(s) 59, 75, 178, 467, 605, 1133
BstNI CCWGG 1 cut(s) 688
BstSCI CCNGG 2 cut(s) 145, 686
BstSFI CTRYAG 1 cut(s) 378
BstUI CGCG 3 cut(s) 169, 782, 1071
BstV1I GCAGC 2 cut(s) 711, 1171
BstV2I GAAGAC 1 cut(s) 126
BstX2I RGATCY 1 cut(s) 740
BstXI CCANNNNNNTGG 1 cut(s) 957
BstYI RGATCY 1 cut(s) 740
BsuI GTATCC 1 cut(s) 996
BsuRI GGCC 3 cut(s) 933, 1082, 1094
BtgI CCRYGG 1 cut(s) 998
BtgZI GCGATG 1 cut(s) 1086
BtsCI GGATG 4 cut(s) 38, 354, 990, 1354
BtsIMutI CAGTG 6 cut(s) 375, 627, 742, 1010, 1032, 1218
BveI ACCTGC 1 cut(s) 1015
Cac8I GCNNGC 2 cut(s) 183, 573
CaiI CAGNNNCTG 1 cut(s) 599
CfoI GCGC 4 cut(s) 161, 169, 782, 1071
Cfr10I RCCGGY 2 cut(s) 181, 200
Cfr13I GGNCC 2 cut(s) 203, 245
CpoI CGGWCCG 1 cut(s) 203
CseI GACGC 2 cut(s) 59, 597
CsiI ACCWGGT 1 cut(s) 686
Csp6I GTAC 4 cut(s) 198, 684, 904, 1015
CspAI ACCGGT 1 cut(s) 200
CspI CGGWCCG 1 cut(s) 203
CviAII CATG 6 cut(s) 30, 703, 918, 963, 999, 1137
CviQI GTAC 4 cut(s) 198, 684, 904, 1015
DdeI CTNAG 3 cut(s) 275, 806, 1269
DinI GGCGCC 1 cut(s) 160
DpnI GATC 5 cut(s) 304, 310, 742, 1228, 1359
DpnII GATC 5 cut(s) 302, 308, 740, 1226, 1357
EaeI YGGCCR 2 cut(s) 1080, 1092
Eam1104I CTCTTC 2 cut(s) 76, 97
EarI CTCTTC 2 cut(s) 76, 97
EciI GGCGGA 1 cut(s) 334
Ecl136II GAGCTC 1 cut(s) 36
Eco130I CCWWGG 3 cut(s) 234, 998, 1147
Eco24I GRGCYC 3 cut(s) 38, 700, 1166
Eco47I GGWCC 2 cut(s) 203, 245
Eco53kI GAGCTC 1 cut(s) 36
EcoICRI GAGCTC 1 cut(s) 36
EcoRII CCWGG 1 cut(s) 686
EcoT14I CCWWGG 3 cut(s) 234, 998, 1147
EcoT38I GRGCYC 3 cut(s) 38, 700, 1166
EgeI GGCGCC 1 cut(s) 160
EheI GGCGCC 1 cut(s) 160
ErhI CCWWGG 3 cut(s) 234, 998, 1147
FaeI CATG 6 cut(s) 33, 706, 921, 966, 1002, 1140
FaqI GGGAC 1 cut(s) 659
FatI CATG 6 cut(s) 29, 702, 917, 962, 998, 1136
FauI CCCGC 1 cut(s) 14
FauNDI CATATG 2 cut(s) 801, 899
Fnu4HI GCNGC 5 cut(s) 6, 170, 438, 725, 1185
FokI GGATG 4 cut(s) 25, 341, 997, 1361
FriOI GRGCYC 3 cut(s) 38, 700, 1166
Fsp4HI GCNGC 5 cut(s) 6, 170, 438, 725, 1185
FspBI CTAG 2 cut(s) 390, 1196
GlaI GCGC 4 cut(s) 160, 168, 781, 1070
GluI GCNGC 5 cut(s) 6, 170, 438, 725, 1185
GsaI CCCAGC 2 cut(s) 437, 1289
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 3 cut(s) 933, 1082, 1094
HapII CCGG 4 cut(s) 147, 156, 182, 201
HgaI GACGC 2 cut(s) 59, 597
HhaI GCGC 4 cut(s) 161, 169, 782, 1071
Hin1I GRCGYC 2 cut(s) 51, 159
Hin1II CATG 6 cut(s) 33, 706, 921, 966, 1002, 1140
Hin6I GCGC 4 cut(s) 159, 167, 780, 1069
HinP1I GCGC 4 cut(s) 159, 167, 780, 1069
HindIII AAGCTT 1 cut(s) 1204
HinfI GANTC 4 cut(s) 85, 208, 543, 731
HpaII CCGG 4 cut(s) 147, 156, 182, 201
HphI GGTGA 3 cut(s) 6, 135, 518
Hpy166II GTNNAC 4 cut(s) 444, 627, 960, 1017
Hpy188I TCNGA 5 cut(s) 207, 497, 595, 1239, 1297
Hpy188III TCNNGA 5 cut(s) 306, 775, 825, 1341, 1355
Hpy8I GTNNAC 4 cut(s) 444, 627, 960, 1017
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 2 cut(s) 368, 1257
HpyCH4III ACNGT 9 cut(s) 18, 197, 265, 379, 448, 631, 1014, 1036, 1213
HpyCH4V TGCA 5 cut(s) 527, 571, 1010, 1064, 1216
HpyF10VI GCNNNNNNNGC 6 cut(s) 59, 75, 178, 467, 605, 1133
HpyF3I CTNAG 3 cut(s) 275, 806, 1269
Hsp92I GRCGYC 2 cut(s) 51, 159
Hsp92II CATG 6 cut(s) 33, 706, 921, 966, 1002, 1140
HspAI GCGC 4 cut(s) 159, 167, 780, 1069
KasI GGCGCC 1 cut(s) 158
KpnI GGTACC 1 cut(s) 907
KroI GCCGGC 1 cut(s) 181
KroNI GCCGGC 1 cut(s) 183
Kzo9I GATC 5 cut(s) 302, 308, 740, 1226, 1357
LguI GCTCTTC 1 cut(s) 76
LmnI GCTCC 7 cut(s) 33, 158, 351, 665, 695, 1111, 1357
Lsp1109I GCAGC 2 cut(s) 711, 1171
LweI GCATC 4 cut(s) 363, 586, 975, 1339
MabI ACCWGGT 1 cut(s) 686
MaeI CTAG 2 cut(s) 390, 1196
MaeIII GTNAC 1 cut(s) 265
MalI GATC 5 cut(s) 304, 310, 742, 1228, 1359
MboI GATC 5 cut(s) 302, 308, 740, 1226, 1357
MboII GAAGA 4 cut(s) 63, 84, 126, 322
MflI RGATCY 1 cut(s) 740
MhlI GDGCHC 3 cut(s) 38, 700, 1166
MluCI AATT 4 cut(s) 188, 394, 531, 1098
Mly113I GGCGCC 1 cut(s) 159
MlyI GAGTC 1 cut(s) 217
MmeI TCCRAC 4 cut(s) 236, 736, 1154, 1262
MnlI CCTC 9 cut(s) 123, 315, 346, 497, 710, 864, 917, 944, 1330
MroNI GCCGGC 1 cut(s) 181
MseI TTAA 3 cut(s) 366, 945, 1208
MslI CAYNNNNRTG 1 cut(s) 955
MspA1I CMGCKG 2 cut(s) 437, 1289
MspI CCGG 4 cut(s) 147, 156, 182, 201
MspR9I CCNGG 2 cut(s) 147, 688
MvaI CCWGG 1 cut(s) 688
MvnI CGCG 3 cut(s) 169, 782, 1071
MwoI GCNNNNNNNGC 6 cut(s) 59, 75, 178, 467, 605, 1133
NaeI GCCGGC 1 cut(s) 183
NarI GGCGCC 1 cut(s) 159
NciI CCSGG 1 cut(s) 147
NcoI CCATGG 1 cut(s) 998
NdeI CATATG 2 cut(s) 801, 899
NdeII GATC 5 cut(s) 302, 308, 740, 1226, 1357
NgoMIV GCCGGC 1 cut(s) 181
NlaIII CATG 6 cut(s) 33, 706, 921, 966, 1002, 1140
NlaIV GGNNCC 6 cut(s) 160, 247, 474, 697, 905, 1113
PciSI GCTCTTC 1 cut(s) 76
PdiI GCCGGC 1 cut(s) 183
PfeI GAWTC 3 cut(s) 85, 543, 731
PflMI CCANNNNNTGG 1 cut(s) 755
PinAI ACCGGT 1 cut(s) 200
PkrI GCNGC 5 cut(s) 7, 171, 439, 726, 1186
PleI GAGTC 1 cut(s) 216
PluTI GGCGCC 1 cut(s) 162
PpsI GAGTC 1 cut(s) 216
Psp124BI GAGCTC 1 cut(s) 38
Psp6I CCWGG 1 cut(s) 686
PspFI CCCAGC 2 cut(s) 433, 1285
PspGI CCWGG 1 cut(s) 686
PspN4I GGNNCC 6 cut(s) 160, 247, 474, 697, 905, 1113
PspPI GGNCC 2 cut(s) 203, 245
PstNI CAGNNNCTG 1 cut(s) 599
PsuI RGATCY 1 cut(s) 740
RsaI GTAC 4 cut(s) 199, 685, 905, 1016
RsaNI GTAC 4 cut(s) 198, 684, 904, 1015
RseI CAYNNNNRTG 1 cut(s) 955
Rsr2I CGGWCCG 1 cut(s) 203
RsrII CGGWCCG 1 cut(s) 203
SacI GAGCTC 1 cut(s) 38
SapI GCTCTTC 1 cut(s) 76
SaqAI TTAA 3 cut(s) 366, 945, 1208
SatI GCNGC 5 cut(s) 6, 170, 438, 725, 1185
Sau3AI GATC 5 cut(s) 302, 308, 740, 1226, 1357
Sau96I GGNCC 2 cut(s) 203, 245
SchI GAGTC 1 cut(s) 217
ScrFI CCNGG 2 cut(s) 147, 688
SduI GDGCHC 3 cut(s) 38, 700, 1166
SexAI ACCWGGT 1 cut(s) 686
SfaNI GCATC 4 cut(s) 363, 586, 975, 1339
SfcI CTRYAG 1 cut(s) 378
SfoI GGCGCC 1 cut(s) 160
SinI GGWCC 2 cut(s) 203, 245
SmiMI CAYNNNNRTG 1 cut(s) 955
SmlI CTYRAG 1 cut(s) 775
SmoI CTYRAG 1 cut(s) 775
Sse9I AATT 4 cut(s) 188, 394, 531, 1098
SspDI GGCGCC 1 cut(s) 158
SspMI CTAG 2 cut(s) 390, 1196
SstI GAGCTC 1 cut(s) 38
StyD4I CCNGG 2 cut(s) 145, 686
StyI CCWWGG 3 cut(s) 234, 998, 1147
TaaI ACNGT 9 cut(s) 18, 197, 265, 379, 448, 631, 1014, 1036, 1213
TaqI TCGA 3 cut(s) 83, 307, 401
TasI AATT 4 cut(s) 188, 394, 531, 1098
TatI WGTACW 1 cut(s) 1014
TauI GCSGC 2 cut(s) 172, 440
TfiI GAWTC 3 cut(s) 85, 543, 731
Tru1I TTAA 3 cut(s) 366, 945, 1208
Tru9I TTAA 3 cut(s) 366, 945, 1208
TscAI CASTG 6 cut(s) 382, 634, 742, 1017, 1039, 1218
TseI GCWGC 3 cut(s) 5, 724, 1184
TspDTI ATGAA 3 cut(s) 788, 1272, 1277
TspRI CASTG 6 cut(s) 382, 634, 742, 1017, 1039, 1218
Van91I CCANNNNNTGG 1 cut(s) 755
VpaK11BI GGWCC 2 cut(s) 203, 245
XspI CTAG 2 cut(s) 390, 1196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.