Rw4G033380

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0016838
Physical Location & Seq
Forward (+)
60499459 .. 60502652
3194 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G033380.1

Sequence Viewer

Length: 1371 bp
ATGGCTGCTCAGTCACCGTGTGGGTGGTCATGGAGCTCATCCACCTACGACGCCACCACCGCCAAATGCTCTTCCAGCTCATCGAATCTCTCTTCGTTTTATACAAAACGACCTCGTTTCACCGCCTGTTCGTCGTCTTCTAAATCACCCGGTAGCTCCGGCGACAAAGCGCGGCTGAAAAAGCAGGCGAAATTGGACGGTACCGGTCCGAGTCCAACCGCATTGGTGAAAACCGCAAAGGGAGATGGACCCAGAAGTGAAAAGACGGTAACAGCCCTGAGCTTCAAGTCCTTGTCTGGGAAGCGATCTCTATGGCGGAGGATATTCTTCGCCTCCAAGAAAGTTAGGAGCATCCTTCTGCTTAATGTAATCACTGTAGTCTATGCTAGTAATTTTTCGATTGTGAAAGAAGTGGAAGCTGTGATAGACCCAGCGGCGTTTACTGTTGTGCGGTTTGCTATGTCGGCGGTTCCATTTATCCCATTTGTTTTCCGAGCAAGAGGTGATGTTGATACTCGTAATGCAGGAATTGAGTTGGGATTCTGGGTTAGTTTAGGGTATCTTATGCAGGCGTTAGGACTGCTAACATCAGATGCTGGTCGTGCGTCATTTCTTTCTATGTTCACTGTGATTATCGTTCCCTTGCTTGATGGAATGTTAGGAGCAATAGTCCCTGCTCGTACCTGGTTTGGAGCCCTCATGTCTATTGTAGGAGTTGGGCTGCTGGAATCCAGTGGATCTCCCCCAAATGTTGGAGATTTGCTGAACTTCTTGAGCGCAGTGTTTTTTGGGGTTCATATGCTTAGAACAGAGCATATATCAAGAAACACAAAAAAAGAAAACTTTTTGCCTCTGCTTGGATATGAGATATGTGTTGTTGCTTTCTTATCAACCATATGGTACCTCATTGGGGCATGGTCTGGTGGTGGCCTCCACGACTTTAACCCATCATTGTGGACATGGGAGTTGTTCTGGGAGTGGATGGTTGTGTTTCCATGGATACCTGCACTGTACACAGGCATTTTCTCCACTGTTTTATGCTTATGGGTAGAGATGGGTGCAATGTGCGATGTATCAGCCACAGAAACGGCCATAATTTATGGTTTAGAGCCTGTCTGGGGTGCTGGTTTCGCATGGTTTCTCCTTGGCGAAAGATGGGGCTCTACTGGTTGGATTGGTGCTGCTCTTGTGCTAGGTGGAAGCTTAACAGTGCAGATATTTGGATCAACATCTCCCACAGAGCCTTCAAAAGATGAAATGAAAGCTAAGAAACTCACTTTCCCAGTGGTTTCGGATAAACACAATGGCTTATCTGCCTCGCCAGTTATAGTCCGTTCCAGAAAGGATGCTCCTGATCTGCGGAGTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

456

Amino Acids

49.47

Weight (kDa)

9.42

Isoelectric Point (pI)

44.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 118 - 241 9.4e-08 EamA-like transporter family
EamA PF00892 252 - 402 1.4e-11 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016838)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g32690
malus_domestica MD16G1026300.v1.1
prunus_persica Prupe.1G327200_v2.0.a1
pyrus_communis pycom16g02220
rosa_chinensis RchiOBHm_Chr4g0441611
rosa_laevigata RLG00000006081
rosa_multiflora Rmu_sc0000353.1_g000024
rosa_roxburghii Rroxscaffold_5G00382440
rosa_rugosa Rorug04G0334900
rosa_samantha Rh4AG386400 Rh4BG400600 Rh4CG415600 Rh4DG393300
rosa_wichuraiana Rw4G033380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1012
Acc65I GGTACC 2 cut(s) 200, 900
AccB1I GGYRCC 2 cut(s) 200, 900
AccB7I CCANNNNNTGG 1 cut(s) 752
AccII CGCG 1 cut(s) 172
AclWI GGATC 2 cut(s) 745, 1231
AcoI YGGCCR 1 cut(s) 1089
AcyI GRCGYC 1 cut(s) 51
AdeI CACNNNGTG 1 cut(s) 20
AfaI GTAC 4 cut(s) 202, 682, 902, 1013
AfiI CCNNNNNNNGG 5 cut(s) 297, 752, 857, 910, 1118
AgeI ACCGGT 1 cut(s) 203
AgsI TTSAA 2 cut(s) 286, 1248
AjnI CCWGG 1 cut(s) 683
AluBI AGCT 7 cut(s) 36, 78, 156, 282, 419, 1203, 1265
AluI AGCT 7 cut(s) 36, 78, 156, 282, 419, 1203, 1265
Alw21I GWGCWC 1 cut(s) 38
AlwI GGATC 2 cut(s) 745, 1231
AlwNI CAGNNNCTG 1 cut(s) 596
AoxI GGCC 2 cut(s) 928, 1089
ApeKI GCWGC 3 cut(s) 5, 721, 1181
AsiGI ACCGGT 1 cut(s) 203
Asp718I GGTACC 2 cut(s) 200, 900
AspLEI GCGC 2 cut(s) 172, 779
AspS9I GGNCC 2 cut(s) 206, 248
AsuC2I CCSGG 1 cut(s) 150
AsuHPI GGTGA 5 cut(s) 6, 112, 138, 238, 515
AvaII GGWCC 2 cut(s) 206, 248
BanI GGYRCC 2 cut(s) 200, 900
BanII GRGCYC 3 cut(s) 38, 697, 1163
BbsI GAAGAC 1 cut(s) 129
Bbv12I GWGCWC 1 cut(s) 38
BbvI GCAGC 2 cut(s) 708, 1168
BccI CCATC 6 cut(s) 239, 644, 955, 976, 1048, 1149
BceAI ACGGC 1 cut(s) 1104
BciT130I CCWGG 1 cut(s) 685
BciVI GTATCC 1 cut(s) 993
BcnI CCSGG 1 cut(s) 150
BfaI CTAG 2 cut(s) 387, 1193
BfmI CTRYAG 1 cut(s) 375
BfuAI ACCTGC 1 cut(s) 1012
BfuI GTATCC 1 cut(s) 993
BisI GCNGC 5 cut(s) 6, 173, 435, 722, 1182
BlsI GCNGC 5 cut(s) 7, 174, 436, 723, 1183
Bme1390I CCNGG 2 cut(s) 150, 685
Bme18I GGWCC 2 cut(s) 206, 248
BmgT120I GGNCC 2 cut(s) 206, 248
BmiI GGNNCC 5 cut(s) 202, 250, 471, 694, 902
BmrFI CCNGG 2 cut(s) 150, 685
BmrI ACTGGG 1 cut(s) 1277
BmsI GCATC 3 cut(s) 360, 583, 1336
BmuI ACTGGG 1 cut(s) 1277
BpiI GAAGAC 1 cut(s) 129
Bpu10I CCTNAGC 1 cut(s) 278
BpuEI CTTGAG 1 cut(s) 793
BpuMI CCSGG 1 cut(s) 150
BsaBI GATNNNNATC 1 cut(s) 1228
BsaHI GRCGYC 1 cut(s) 51
BsaJI CCNNGG 2 cut(s) 995, 1144
BsaWI WCCGGW 1 cut(s) 203
BsaXI ACNNNNNCTCC 2 cut(s) 654, 684
Bsc4I CCNNNNNNNGG 5 cut(s) 297, 752, 857, 910, 1118
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 4 cut(s) 732, 1171, 1283, 1322
Bse3DI GCAATG 1 cut(s) 1068
Bse8I GATNNNNATC 1 cut(s) 1228
BseBI CCWGG 1 cut(s) 685
BseDI CCNNGG 2 cut(s) 995, 1144
BseGI GGATG 4 cut(s) 38, 351, 987, 1351
BseJI GATNNNNATC 1 cut(s) 1228
BseLI CCNNNNNNNGG 5 cut(s) 297, 752, 857, 910, 1118
BseMI GCAATG 1 cut(s) 1068
BseMII CTCAG 2 cut(s) 23, 269
BseNI ACTGG 4 cut(s) 732, 1171, 1283, 1322
BseXI GCAGC 2 cut(s) 708, 1168
BseYI CCCAGC 1 cut(s) 430
BsgI GTGCAG 2 cut(s) 990, 1232
Bsh1236I CGCG 1 cut(s) 172
BshFI GGCC 2 cut(s) 930, 1091
BshNI GGYRCC 2 cut(s) 200, 900
BshTI ACCGGT 1 cut(s) 203
BsiHKAI GWGCWC 1 cut(s) 38
BsiSI CCGG 3 cut(s) 150, 159, 204
BslFI GGGAC 1 cut(s) 656
BslI CCNNNNNNNGG 5 cut(s) 297, 752, 857, 910, 1118
BsmFI GGGAC 1 cut(s) 656
BsnI GGCC 2 cut(s) 930, 1091
Bsp1286I GDGCHC 3 cut(s) 38, 697, 1163
Bsp1407I TGTACA 1 cut(s) 1011
Bsp143I GATC 4 cut(s) 305, 737, 1223, 1354
Bsp19I CCATGG 1 cut(s) 995
BspANI GGCC 2 cut(s) 930, 1091
BspCNI CTCAG 2 cut(s) 22, 270
BspFNI CGCG 1 cut(s) 172
BspLI GGNNCC 5 cut(s) 202, 250, 471, 694, 902
BspMI ACCTGC 1 cut(s) 1012
BspPI GGATC 2 cut(s) 745, 1231
BspQI GCTCTTC 1 cut(s) 76
BspT107I GGYRCC 2 cut(s) 200, 900
BsrDI GCAATG 1 cut(s) 1068
BsrFI RCCGGY 1 cut(s) 203
BsrGI TGTACA 1 cut(s) 1011
BsrI ACTGG 4 cut(s) 732, 1171, 1283, 1322
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 2 cut(s) 995, 1144
BssMI GATC 4 cut(s) 305, 737, 1223, 1354
BssNI GRCGYC 1 cut(s) 51
BssT1I CCWWGG 2 cut(s) 995, 1144
Bst2UI CCWGG 1 cut(s) 685
Bst4CI ACNGT 9 cut(s) 18, 200, 268, 376, 445, 628, 1011, 1033, 1210
Bst6I CTCTTC 2 cut(s) 76, 97
BstACI GRCGYC 1 cut(s) 51
BstAUI TGTACA 1 cut(s) 1011
BstC8I GCNNGC 2 cut(s) 186, 570
BstDEI CTNAG 4 cut(s) 9, 278, 803, 1266
BstDSI CCRYGG 1 cut(s) 995
BstF5I GGATG 4 cut(s) 38, 351, 987, 1351
BstFNI CGCG 1 cut(s) 172
BstHHI GCGC 2 cut(s) 172, 779
BstKTI GATC 4 cut(s) 308, 740, 1226, 1357
BstMBI GATC 4 cut(s) 305, 737, 1223, 1354
BstMWI GCNNNNNNNGC 6 cut(s) 59, 75, 181, 464, 602, 1130
BstNI CCWGG 1 cut(s) 685
BstSCI CCNGG 2 cut(s) 148, 683
BstSFI CTRYAG 1 cut(s) 375
BstUI CGCG 1 cut(s) 172
BstV1I GCAGC 2 cut(s) 708, 1168
BstV2I GAAGAC 1 cut(s) 129
BstX2I RGATCY 1 cut(s) 737
BstXI CCANNNNNNTGG 1 cut(s) 954
BstYI RGATCY 1 cut(s) 737
BsuI GTATCC 1 cut(s) 993
BsuRI GGCC 2 cut(s) 930, 1091
BtgI CCRYGG 1 cut(s) 995
BtgZI GCGATG 1 cut(s) 1083
BtsCI GGATG 4 cut(s) 38, 351, 987, 1351
BtsI GCAGTG 1 cut(s) 786
BtsIMutI CAGTG 8 cut(s) 372, 624, 739, 786, 1007, 1029, 1215, 1290
BveI ACCTGC 1 cut(s) 1012
Cac8I GCNNGC 2 cut(s) 186, 570
CaiI CAGNNNCTG 1 cut(s) 596
CfoI GCGC 2 cut(s) 172, 779
Cfr10I RCCGGY 1 cut(s) 203
Cfr13I GGNCC 2 cut(s) 206, 248
CpoI CGGWCCG 1 cut(s) 206
CseI GACGC 2 cut(s) 59, 594
CsiI ACCWGGT 1 cut(s) 683
Csp6I GTAC 4 cut(s) 201, 681, 901, 1012
CspAI ACCGGT 1 cut(s) 203
CspI CGGWCCG 1 cut(s) 206
CviAII CATG 6 cut(s) 30, 700, 915, 960, 996, 1134
CviQI GTAC 4 cut(s) 201, 681, 901, 1012
DdeI CTNAG 4 cut(s) 9, 278, 803, 1266
DpnI GATC 4 cut(s) 307, 739, 1225, 1356
DpnII GATC 4 cut(s) 305, 737, 1223, 1354
DraIII CACNNNGTG 1 cut(s) 20
EaeI YGGCCR 1 cut(s) 1089
Eam1104I CTCTTC 2 cut(s) 76, 97
EarI CTCTTC 2 cut(s) 76, 97
EciI GGCGGA 1 cut(s) 331
Ecl136II GAGCTC 1 cut(s) 36
Eco130I CCWWGG 2 cut(s) 995, 1144
Eco24I GRGCYC 3 cut(s) 38, 697, 1163
Eco47I GGWCC 2 cut(s) 206, 248
Eco53kI GAGCTC 1 cut(s) 36
EcoICRI GAGCTC 1 cut(s) 36
EcoRII CCWGG 1 cut(s) 683
EcoT14I CCWWGG 2 cut(s) 995, 1144
EcoT38I GRGCYC 3 cut(s) 38, 697, 1163
ErhI CCWWGG 2 cut(s) 995, 1144
FaeI CATG 6 cut(s) 33, 703, 918, 963, 999, 1137
FaqI GGGAC 1 cut(s) 656
FatI CATG 6 cut(s) 29, 699, 914, 959, 995, 1133
FauNDI CATATG 2 cut(s) 798, 896
Fnu4HI GCNGC 5 cut(s) 6, 173, 435, 722, 1182
FokI GGATG 4 cut(s) 25, 338, 994, 1358
FriOI GRGCYC 3 cut(s) 38, 697, 1163
Fsp4HI GCNGC 5 cut(s) 6, 173, 435, 722, 1182
FspBI CTAG 2 cut(s) 387, 1193
GlaI GCGC 2 cut(s) 171, 778
GluI GCNGC 5 cut(s) 6, 173, 435, 722, 1182
GsaI CCCAGC 1 cut(s) 434
HaeIII GGCC 2 cut(s) 930, 1091
HapII CCGG 3 cut(s) 150, 159, 204
HgaI GACGC 2 cut(s) 59, 594
HhaI GCGC 2 cut(s) 172, 779
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 6 cut(s) 33, 703, 918, 963, 999, 1137
Hin6I GCGC 2 cut(s) 170, 777
HinP1I GCGC 2 cut(s) 170, 777
HindIII AAGCTT 1 cut(s) 1201
HinfI GANTC 4 cut(s) 85, 211, 540, 728
HpaII CCGG 3 cut(s) 150, 159, 204
HphI GGTGA 5 cut(s) 6, 112, 138, 238, 515
Hpy166II GTNNAC 4 cut(s) 441, 624, 957, 1014
Hpy188I TCNGA 4 cut(s) 210, 494, 592, 1294
Hpy188III TCNNGA 4 cut(s) 772, 822, 1338, 1352
Hpy8I GTNNAC 4 cut(s) 441, 624, 957, 1014
Hpy99I CGWCG 2 cut(s) 53, 136
HpyAV CCTTC 2 cut(s) 365, 1254
HpyCH4III ACNGT 9 cut(s) 18, 200, 268, 376, 445, 628, 1011, 1033, 1210
HpyCH4V TGCA 5 cut(s) 524, 568, 1007, 1061, 1213
HpyF10VI GCNNNNNNNGC 6 cut(s) 59, 75, 181, 464, 602, 1130
HpyF3I CTNAG 4 cut(s) 9, 278, 803, 1266
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 6 cut(s) 33, 703, 918, 963, 999, 1137
HspAI GCGC 2 cut(s) 170, 777
KpnI GGTACC 2 cut(s) 204, 904
Kzo9I GATC 4 cut(s) 305, 737, 1223, 1354
LguI GCTCTTC 1 cut(s) 76
LmnI GCTCC 6 cut(s) 33, 161, 348, 662, 692, 1354
Lsp1109I GCAGC 2 cut(s) 708, 1168
LweI GCATC 3 cut(s) 360, 583, 1336
MabI ACCWGGT 1 cut(s) 683
MaeI CTAG 2 cut(s) 387, 1193
MaeIII GTNAC 2 cut(s) 12, 268
MalI GATC 4 cut(s) 307, 739, 1225, 1356
MboI GATC 4 cut(s) 305, 737, 1223, 1354
MboII GAAGA 4 cut(s) 63, 84, 129, 319
MflI RGATCY 1 cut(s) 737
MhlI GDGCHC 3 cut(s) 38, 697, 1163
MluCI AATT 4 cut(s) 191, 391, 528, 1095
MlyI GAGTC 1 cut(s) 220
MmeI TCCRAC 3 cut(s) 239, 733, 1151
MnlI CCTC 9 cut(s) 123, 312, 343, 494, 707, 861, 914, 941, 1327
MseI TTAA 3 cut(s) 363, 942, 1205
MslI CAYNNNNRTG 1 cut(s) 952
MspA1I CMGCKG 1 cut(s) 434
MspI CCGG 3 cut(s) 150, 159, 204
MspR9I CCNGG 2 cut(s) 150, 685
MvaI CCWGG 1 cut(s) 685
MvnI CGCG 1 cut(s) 172
MwoI GCNNNNNNNGC 6 cut(s) 59, 75, 181, 464, 602, 1130
NciI CCSGG 1 cut(s) 150
NcoI CCATGG 1 cut(s) 995
NdeI CATATG 2 cut(s) 798, 896
NdeII GATC 4 cut(s) 305, 737, 1223, 1354
NlaIII CATG 6 cut(s) 33, 703, 918, 963, 999, 1137
NlaIV GGNNCC 5 cut(s) 202, 250, 471, 694, 902
NmuCI GTSAC 1 cut(s) 12
PciSI GCTCTTC 1 cut(s) 76
PfeI GAWTC 3 cut(s) 85, 540, 728
PflMI CCANNNNNTGG 1 cut(s) 752
PinAI ACCGGT 1 cut(s) 203
PkrI GCNGC 5 cut(s) 7, 174, 436, 723, 1183
PleI GAGTC 1 cut(s) 219
PpsI GAGTC 1 cut(s) 219
Psp124BI GAGCTC 1 cut(s) 38
Psp6I CCWGG 1 cut(s) 683
PspFI CCCAGC 1 cut(s) 430
PspGI CCWGG 1 cut(s) 683
PspN4I GGNNCC 5 cut(s) 202, 250, 471, 694, 902
PspPI GGNCC 2 cut(s) 206, 248
PstNI CAGNNNCTG 1 cut(s) 596
PsuI RGATCY 1 cut(s) 737
RsaI GTAC 4 cut(s) 202, 682, 902, 1013
RsaNI GTAC 4 cut(s) 201, 681, 901, 1012
RseI CAYNNNNRTG 1 cut(s) 952
Rsr2I CGGWCCG 1 cut(s) 206
RsrII CGGWCCG 1 cut(s) 206
SacI GAGCTC 1 cut(s) 38
SapI GCTCTTC 1 cut(s) 76
SaqAI TTAA 3 cut(s) 363, 942, 1205
SatI GCNGC 5 cut(s) 6, 173, 435, 722, 1182
Sau3AI GATC 4 cut(s) 305, 737, 1223, 1354
Sau96I GGNCC 2 cut(s) 206, 248
SchI GAGTC 1 cut(s) 220
ScrFI CCNGG 2 cut(s) 150, 685
SduI GDGCHC 3 cut(s) 38, 697, 1163
SexAI ACCWGGT 1 cut(s) 683
SfaNI GCATC 3 cut(s) 360, 583, 1336
SfcI CTRYAG 1 cut(s) 375
SinI GGWCC 2 cut(s) 206, 248
SmiMI CAYNNNNRTG 1 cut(s) 952
SmlI CTYRAG 1 cut(s) 772
SmoI CTYRAG 1 cut(s) 772
Sse9I AATT 4 cut(s) 191, 391, 528, 1095
SspMI CTAG 2 cut(s) 387, 1193
SstI GAGCTC 1 cut(s) 38
StyD4I CCNGG 2 cut(s) 148, 683
StyI CCWWGG 2 cut(s) 995, 1144
TaaI ACNGT 9 cut(s) 18, 200, 268, 376, 445, 628, 1011, 1033, 1210
TaqI TCGA 2 cut(s) 83, 398
TasI AATT 4 cut(s) 191, 391, 528, 1095
TatI WGTACW 1 cut(s) 1011
TauI GCSGC 2 cut(s) 175, 437
TfiI GAWTC 3 cut(s) 85, 540, 728
Tru1I TTAA 3 cut(s) 363, 942, 1205
Tru9I TTAA 3 cut(s) 363, 942, 1205
TscAI CASTG 8 cut(s) 379, 631, 739, 786, 1014, 1036, 1215, 1290
TseFI GTSAC 1 cut(s) 12
TseI GCWGC 3 cut(s) 5, 721, 1181
Tsp45I GTSAC 1 cut(s) 12
TspDTI ATGAA 3 cut(s) 785, 1269, 1274
TspGWI ACGGA 1 cut(s) 1322
TspRI CASTG 8 cut(s) 379, 631, 739, 786, 1014, 1036, 1215, 1290
Van91I CCANNNNNTGG 1 cut(s) 752
VpaK11BI GGWCC 2 cut(s) 206, 248
XspI CTAG 2 cut(s) 387, 1193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.