MD16G1036900.v1.1

elongator complex protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009434
Physical Location & Seq
Reverse (-)
2657034 .. 2661236
4203 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1036900.v1.1.491

Sequence Viewer

Length: 2529 bp
ATGTCTAGCGGAGATGGTCGAGGAGGAGGGCGTGGGGTTGGAGTGAAGGGAGTGTTCATAGGAGCAGGCTGCAACAGAATCGTCAACAACTGTTCGTGGGGTGCTTGTGATTTAGTCGCTTTCGGTGCCCAAAACGCCGTTGCTATCTTCAATCCCGAGACTGCTCAAATTTGGACTACGCTTCCAGGTCACAAGGCTGCTGTGAATTGCACGCAGTGGCTCCCAAGCAACAAGTTTGCATTCAGAGCAAAAAAGTTGGACCGGCATTATTTGCTCTCTGGAGATGCTGCTGGTGAAATTATTTTGTGGGAGTACTCTGTTCTTGAAGGAAAGTGGAGGTATGTGCAACAACTACCTCAGTTACACAAGAAGGGTGTCACATGCATAACTGGAATTATGGTTTCTCAAACTGAGGCAGTCTTTGCCTCTACGTCTTCCGATAGTATGGTTTATATATGGGAGGTTGTGTTTCCATCAACTAGTGGAGGTGTCTGTGAACTGTTGCATCTGGATTCTCTCTCTGTTGGTACAAAACCCATGGTAGCGCTTTCATTATCAGAGTTGCCTGGAAGTACTGGGCACTTAGTCCTGGCAATGGGAGGACTGGATAACAAAATTCACCTTTACAGCGGGGAGAGGAGAGGAAAGTTTGTTCGAGGGTGCGAGTTGAAAGGGCATACAGATTGGATCAGAAGTCTGGATTTCGCATTACCTACGTGCACCAATGGTGAGGCAAGCAACGTACTACTTGTTAGTTCATCTCAAGACAGAGGCATACGCATATGGAAGATGGCTTTAAAGGAGTCTTTGGACAGCAACCAGAGTGCTTACCGGAAAGAAAAAGTAAGCTTAGCATCTTATATTGAAGGCCCTGTACTTATAGCTGGCACGACGTCATATCAGATATCATTGGAATCTCTTCTAATTGGACATGAAGATTGGGTGTATTCAGTGGAGTGGCAACCCCCGTCAAATGCATCTTCTGAAGGGATTGCCTACTGTCAACCCCAAAGCATCTTATCTGCATCTATGGACAAGACAATGATGATATGGAAACCCGAAAAGACGTCTGGTATCTGGATGAATGTTGTTACTGTTGGAGAACTCAGTCATTGTGCTCTAGGGTTTTATGGTGGCCACTGGAGCCCTAATGGAGATTCAATATTAGCACATGGATATGGTGGATCTTTCCATCTCTGGAAAAATGTCGGCACTGAGTATGAAAATTGGCAACCACAAAAGGTTCCATCTGGGCATTTTGCAGCAATAGCAGATATTGCATGGGGAAGATCTGGTCAATACTTGCTGTCAGTCAGTCATGATCAGACAACTCGAATTTTTGCTCCTTGGAAAAATGAGGCATCTCCTAGAGACGAGGAGTCTTGGCATGAAATTGCTCGGCCTCAAGTTCATGGTCATGATATTAACTGTGTGGCCATCATCCAAGGAAAGGGGAACCATCGATTTGTCAGCGGAGCTGATGAGAAAGTTGCCAGAGTGTTTGAAGCTCCATTGTCTTTCTTGAAGACATTGGATCATGCCATTTCACAAACATCTGACTTTTCAGATGATTCCCAAGTAGGTGTTCAGATTTTGGGTGCAAATATGTCTGCTCTTGGGCTATCACAGAAACCTATTTATGTTCATGCTGAGCAGCATACCCCAGACAAGAATGTGAATGATGGCCTCGACACACTTGAAACCATTCCTGATGCTGTTCCAGTTGTGTTGACTGAACCTCCCATTGAAGATCAACTGGCATGGCATACACTATGGCCAGAGTCACACAAACTTTACGGTCATGGGAATGAGCTGTTTGCTCTGTGCAGTGATCATGATGGGAAGCTTGTTGCTTCTTCATGTAAGGCCCAATCAGCAGCGGTAGCAGAAATATGGCTATGGCAAATTGGTTCATGGAAAGCAGTTGGTCGCTTGCAGTCTCATACTTTGACAGTGACACAAATGGAATTCTCTCATGATGACAAATTCCTGTTGGCTGTATCAAGGGATCGCCAGTTCTCTGTATTTTCAATCGATAAAGCAGGCACCGATGAAATTACTTACCAGCTCGTAGCAAAGCAGGAGGCACACAAAAGAATCATATGGGCATGCTCTTGGAATCCATACGGGTACGAATTTGCCACAGGCTCGAGGGACAAGACAGTGAAGATCTGGACTGTGGAAAATGAGTCTTCAGTGAAGCTGCTCGCGACTCTTCCCCAGTTCAGTAGTAGCGTCATGGCCCTATCTTGGGTCGGTCTTGATTGCAACAGCAACGAAGGGCTCCTCGCAGTTGGAATGGAAAACGGACTCATTGAACTGTGGAATCTATCTGTTAAAAGATCTGATGATGGAGTAGCAGGCGCAGTTGCTTCCCTTGTCGTACGGCTTGAGCCGTTGATGTGCCATGTCTCTGCTGTAAACCGTTTGGCATGGAGAAACTGCAAGAATGAAGATTCCGGTAGCTTACAGCTCGCTTCTTGCGGGGCAGATCAATGTGTGAGAGTGTTCCAGGTTAACATTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000123 GO:0000502 GO:0001101 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006354 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006950 GO:0006979 GO:0008023 GO:0008047 GO:0008150 GO:0008152 GO:0008284 GO:0009058 GO:0009059 GO:0009719 GO:0009725 GO:0009737 GO:0009892 GO:0009893 GO:0009987 GO:0010033 GO:0010467 GO:0010604 GO:0010950 GO:0010952 GO:0016043 GO:0016070 GO:0016504 GO:0018130 GO:0019222 GO:0019438 GO:0022607 GO:0030162 GO:0030234 GO:0031248 GO:0031323 GO:0031325 GO:0031347 GO:0031349 GO:0031537 GO:0031538 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032774 GO:0032991 GO:0033588 GO:0033993 GO:0034622 GO:0034641 GO:0034645 GO:0034654 GO:0042127 GO:0042221 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043248 GO:0043933 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045862 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0048584 GO:0048831 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051239 GO:0051246 GO:0051247 GO:0051336 GO:0051345 GO:0052547 GO:0052548 GO:0060255 GO:0061133 GO:0061134 GO:0061135 GO:0065003 GO:0065007 GO:0065009 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0090304 GO:0097305 GO:0097659 GO:0098772 GO:1901360 GO:1901362 GO:1901576 GO:1901700 GO:1902493 GO:1902494 GO:1905368 GO:1905369 GO:1990234 GO:2000024 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

843

Amino Acids

92.23

Weight (kDa)

5.89

Isoelectric Point (pI)

35.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 28 - 155 3.1e-06 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 39 - 155 2.5e-06 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 40 - 155 1.1e-06 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 87 - 287 2.2e-08 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 197 - 304 2.3e-07 THOC3 beta-propeller domain
Beta-prop_THOC3 PF25174 307 - 526 1.4e-08 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 381 - 502 5.5e-06 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 588 - 770 2.8e-09 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 594 - 680 2.3e-06 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 597 - 727 1.6e-12 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 599 - 778 7.3e-18 WDR5 beta-propeller domain
Beta-prop_EML PF23409 600 - 789 9.7e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd PF25172 627 - 730 9.4e-06 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 629 - 777 5.7e-10 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 631 - 778 7.1e-12 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 632 - 782 4.7e-07 TEP-1 second beta-propeller
EIF3I PF24805 645 - 738 7.7e-06 EIF3I
WD40 PF00400 690 - 725 4.4e-07 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 697 - 782 1.6e-09 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 896, 1070
AccB1I GGYRCC 2 cut(s) 125, 2045
AccII CGCG 1 cut(s) 2210
AciI CCGC 5 cut(s) 9, 630, 1473, 1880, 2484
AclWI GGATC 4 cut(s) 695, 1192, 1542, 2016
AcoI YGGCCR 3 cut(s) 1135, 1434, 1775
AcsI RAATTY 6 cut(s) 168, 615, 1335, 1967, 1985, 2135
AcuI CTGAAG 2 cut(s) 1005, 2178
AcyI GRCGYC 2 cut(s) 893, 1067
AdeI CACNNNGTG 1 cut(s) 216
AfaI GTAC 7 cut(s) 314, 529, 574, 744, 876, 2132, 2385
AfeI AGCGCT 1 cut(s) 546
AfiI CCNNNNNNNGG 4 cut(s) 595, 1450, 2250, 2251
AhdI GACNNNNNGTC 1 cut(s) 1378
AhlI ACTAGT 1 cut(s) 479
AjnI CCWGG 4 cut(s) 184, 565, 588, 2511
AjuI GAANNNNNNNTTGG 2 cut(s) 1569, 1601
AloI GAACNNNNNNTCC 4 cut(s) 636, 668, 2000, 2032
Alw21I GWGCWC 2 cut(s) 722, 1120
Alw26I GTCTC 4 cut(s) 152, 1365, 1944, 2416
Alw44I GTGCAC 1 cut(s) 718
AlwI GGATC 4 cut(s) 695, 1192, 1542, 2016
Ama87I CYCGRG 2 cut(s) 155, 2149
Aor51HI AGCGCT 1 cut(s) 546
AoxI GGCC 8 cut(s) 868, 1135, 1400, 1434, 1684, 1775, 1866, 2241
ApaLI GTGCAC 1 cut(s) 718
ApeKI GCWGC 7 cut(s) 69, 197, 287, 1262, 1654, 1877, 2203
ApoI RAATTY 6 cut(s) 168, 615, 1335, 1967, 1985, 2135
Asp700I GAANNNNTTC 2 cut(s) 918, 1704
AspLEI GCGC 2 cut(s) 547, 2366
AspS9I GGNCC 4 cut(s) 259, 869, 1867, 2242
AsuHPI GGTGA 3 cut(s) 305, 611, 740
AvaI CYCGRG 2 cut(s) 155, 2149
AvaII GGWCC 1 cut(s) 259
BaeGI GKGCMC 3 cut(s) 130, 582, 722
BalI TGGCCA 3 cut(s) 1137, 1436, 1777
BanI GGYRCC 2 cut(s) 125, 2045
BanII GRGCYC 2 cut(s) 1148, 2286
BarI GAAGNNNNNNTAC 2 cut(s) 858, 890
BbsI GAAGAC 3 cut(s) 426, 1532, 2184
Bbv12I GWGCWC 2 cut(s) 722, 1120
BbvI GCAGC 7 cut(s) 56, 184, 274, 1274, 1666, 1889, 2190
BceAI ACGGC 3 cut(s) 122, 2380, 2402
BcgI CGANNNNNNTGC 2 cut(s) 61, 95
BciT130I CCWGG 4 cut(s) 186, 567, 590, 2513
BclI TGATCA 2 cut(s) 1321, 1831
BcoDI GTCTC 4 cut(s) 152, 1365, 1944, 2416
BcuI ACTAGT 1 cut(s) 479
BfaI CTAG 4 cut(s) 6, 480, 1121, 1368
BfoI RGCGCY 1 cut(s) 548
BglII AGATCT 3 cut(s) 1289, 2169, 2342
BisI GCNGC 7 cut(s) 70, 198, 288, 1263, 1655, 1878, 2204
BlpI GCTNAGC 2 cut(s) 850, 1650
BlsI GCNGC 7 cut(s) 71, 199, 289, 1264, 1656, 1879, 2205
BmcAI AGTACT 2 cut(s) 314, 574
Bme1390I CCNGG 4 cut(s) 186, 567, 590, 2513
Bme18I GGWCC 1 cut(s) 259
BmeRI GACNNNNNGTC 1 cut(s) 1378
BmeT110I CYCGRG 2 cut(s) 155, 2149
BmgT120I GGNCC 4 cut(s) 259, 869, 1867, 2242
BmiI GGNNCC 7 cut(s) 127, 221, 1145, 1245, 1457, 2047, 2285
BmrFI CCNGG 4 cut(s) 186, 567, 590, 2513
BmrI ACTGGG 2 cut(s) 585, 2215
BmsI GCATC 8 cut(s) 274, 514, 863, 986, 1023, 1034, 1370, 1702
BmuI ACTGGG 2 cut(s) 585, 2215
BpiI GAAGAC 3 cut(s) 426, 1532, 2184
BpmI CTGGAG 2 cut(s) 300, 1162
Bpu1102I GCTNAGC 2 cut(s) 850, 1650
BpuEI CTTGAG 3 cut(s) 747, 1389, 2411
Bsa29I ATCGAT 2 cut(s) 1462, 2034
BsaAI YACGTR 1 cut(s) 717
BsaHI GRCGYC 2 cut(s) 893, 1067
BsaJI CCNNGG 3 cut(s) 537, 1346, 1444
BsaWI WCCGGW 2 cut(s) 831, 2459
BsaXI ACNNNNNCTCC 4 cut(s) 452, 482, 1723, 1753
Bsc4I CCNNNNNNNGG 4 cut(s) 595, 1450, 2250, 2251
Bse118I RCCGGY 1 cut(s) 261
Bse1I ACTGG 8 cut(s) 394, 580, 609, 1145, 1721, 1761, 2014, 2221
Bse3DI GCAATG 1 cut(s) 600
BseBI CCWGG 4 cut(s) 186, 567, 590, 2513
BseCI ATCGAT 2 cut(s) 1462, 2034
BseDI CCNNGG 3 cut(s) 537, 1346, 1444
BseGI GGATG 2 cut(s) 1086, 1440
BseLI CCNNNNNNNGG 4 cut(s) 595, 1450, 2250, 2251
BseMI GCAATG 1 cut(s) 600
BseMII CTCAG 5 cut(s) 371, 402, 1120, 1206, 1641
BseNI ACTGG 8 cut(s) 394, 580, 609, 1145, 1721, 1761, 2014, 2221
BseRI GAGGAG 5 cut(s) 36, 39, 652, 1391, 2276
BseSI GKGCMC 3 cut(s) 130, 582, 722
BseXI GCAGC 7 cut(s) 56, 184, 274, 1274, 1666, 1889, 2190
BsgI GTGCAG 1 cut(s) 1846
Bsh1236I CGCG 1 cut(s) 2210
BshFI GGCC 8 cut(s) 870, 1137, 1402, 1436, 1686, 1777, 1868, 2243
BshNI GGYRCC 2 cut(s) 125, 2045
BshVI ATCGAT 2 cut(s) 1462, 2034
BsiHKAI GWGCWC 2 cut(s) 722, 1120
BsiHKCI CYCGRG 2 cut(s) 155, 2149
BsiSI CCGG 3 cut(s) 262, 832, 2460
BsiWI CGTACG 1 cut(s) 2383
BslFI GGGAC 1 cut(s) 2168
BslI CCNNNNNNNGG 4 cut(s) 595, 1450, 2250, 2251
BsmAI GTCTC 4 cut(s) 152, 1365, 1944, 2416
BsmBI CGTCTC 1 cut(s) 1365
BsmFI GGGAC 1 cut(s) 2168
BsmI GAATGC 1 cut(s) 239
BsnI GGCC 8 cut(s) 870, 1137, 1402, 1436, 1686, 1777, 1868, 2243
BsoBI CYCGRG 2 cut(s) 155, 2149
Bsp1286I GDGCHC 6 cut(s) 130, 582, 722, 1120, 1148, 2286
Bsp1720I GCTNAGC 2 cut(s) 850, 1650
Bsp19I CCATGG 1 cut(s) 537
Bsp68I TCGCGA 1 cut(s) 2210
BspACI CCGC 5 cut(s) 9, 630, 1473, 1880, 2484
BspANI GGCC 8 cut(s) 870, 1137, 1402, 1436, 1686, 1777, 1868, 2243
BspCNI CTCAG 5 cut(s) 370, 403, 1119, 1207, 1642
BspDI ATCGAT 2 cut(s) 1462, 2034
BspFNI CGCG 1 cut(s) 2210
BspHI TCATGA 4 cut(s) 1318, 1417, 1834, 1975
BspLI GGNNCC 7 cut(s) 127, 221, 1145, 1245, 1457, 2047, 2285
BspPI GGATC 4 cut(s) 695, 1192, 1542, 2016
BspT107I GGYRCC 2 cut(s) 125, 2045
BsrDI GCAATG 1 cut(s) 600
BsrFI RCCGGY 1 cut(s) 261
BsrI ACTGG 8 cut(s) 394, 580, 609, 1145, 1721, 1761, 2014, 2221
BssAI RCCGGY 1 cut(s) 261
BssECI CCNNGG 3 cut(s) 537, 1346, 1444
BssNI GRCGYC 2 cut(s) 893, 1067
BssT1I CCWWGG 3 cut(s) 537, 1346, 1444
Bst2UI CCWGG 4 cut(s) 186, 567, 590, 2513
Bst6I CTCTTC 2 cut(s) 924, 2220
BstACI GRCGYC 2 cut(s) 893, 1067
BstAPI GCANNNNNTGC 3 cut(s) 271, 422, 1277
BstBAI YACGTR 1 cut(s) 717
BstDEI CTNAG 7 cut(s) 357, 411, 583, 850, 1106, 1215, 1650
BstDSI CCRYGG 1 cut(s) 537
BstF5I GGATG 2 cut(s) 1086, 1440
BstFNI CGCG 1 cut(s) 2210
BstH2I RGCGCY 1 cut(s) 548
BstHHI GCGC 2 cut(s) 547, 2366
BstMAI GTCTC 4 cut(s) 152, 1365, 1944, 2416
BstNI CCWGG 4 cut(s) 186, 567, 590, 2513
BstNSI RCATGY 2 cut(s) 384, 2112
BstSCI CCNGG 4 cut(s) 184, 565, 588, 2511
BstSLI GKGCMC 3 cut(s) 130, 582, 722
BstUI CGCG 1 cut(s) 2210
BstV1I GCAGC 7 cut(s) 56, 184, 274, 1274, 1666, 1889, 2190
BstV2I GAAGAC 3 cut(s) 426, 1532, 2184
BstX2I RGATCY 4 cut(s) 1184, 1289, 2169, 2342
BstYI RGATCY 4 cut(s) 1184, 1289, 2169, 2342
Bsu15I ATCGAT 2 cut(s) 1462, 2034
BsuRI GGCC 8 cut(s) 870, 1137, 1402, 1436, 1686, 1777, 1868, 2243
BsuTUI ATCGAT 2 cut(s) 1462, 2034
BtgI CCRYGG 1 cut(s) 537
BtsCI GGATG 2 cut(s) 1086, 1440
BtsI GCAGTG 2 cut(s) 221, 1834
BtsIMutI CAGTG 8 cut(s) 221, 957, 1138, 1212, 1834, 1959, 2169, 2202
BtuMI TCGCGA 1 cut(s) 2210
CciI TCATGA 4 cut(s) 1318, 1417, 1834, 1975
CfoI GCGC 2 cut(s) 547, 2366
Cfr10I RCCGGY 1 cut(s) 261
Cfr13I GGNCC 4 cut(s) 259, 869, 1867, 2242
ClaI ATCGAT 2 cut(s) 1462, 2034
CseI GACGC 1 cut(s) 2224
Csp6I GTAC 7 cut(s) 313, 528, 573, 743, 875, 2131, 2384
CviQI GTAC 7 cut(s) 313, 528, 573, 743, 875, 2131, 2384
DdeI CTNAG 7 cut(s) 357, 411, 583, 850, 1106, 1215, 1650
DraI TTTAAA 1 cut(s) 798
DraIII CACNNNGTG 1 cut(s) 216
DriI GACNNNNNGTC 1 cut(s) 1378
EaeI YGGCCR 3 cut(s) 1135, 1434, 1775
Eam1104I CTCTTC 2 cut(s) 924, 2220
Eam1105I GACNNNNNGTC 1 cut(s) 1378
EarI CTCTTC 2 cut(s) 924, 2220
Eco130I CCWWGG 3 cut(s) 537, 1346, 1444
Eco24I GRGCYC 2 cut(s) 1148, 2286
Eco32I GATATC 1 cut(s) 906
Eco47I GGWCC 1 cut(s) 259
Eco47III AGCGCT 1 cut(s) 546
Eco57I CTGAAG 2 cut(s) 1005, 2178
Eco88I CYCGRG 2 cut(s) 155, 2149
EcoO109I RGGNCCY 1 cut(s) 869
EcoRI GAATTC 1 cut(s) 1967
EcoRII CCWGG 4 cut(s) 184, 565, 588, 2511
EcoRV GATATC 1 cut(s) 906
EcoT14I CCWWGG 3 cut(s) 537, 1346, 1444
EcoT22I ATGCAT 2 cut(s) 386, 979
EcoT38I GRGCYC 2 cut(s) 1148, 2286
ErhI CCWWGG 3 cut(s) 537, 1346, 1444
Esp3I CGTCTC 1 cut(s) 1365
FaqI GGGAC 1 cut(s) 2168
FauI CCCGC 2 cut(s) 623, 2477
FauNDI CATATG 2 cut(s) 782, 2102
FbaI TGATCA 2 cut(s) 1321, 1831
Fnu4HI GCNGC 7 cut(s) 70, 198, 288, 1263, 1655, 1878, 2204
FokI GGATG 2 cut(s) 1093, 1427
FriOI GRGCYC 2 cut(s) 1148, 2286
Fsp4HI GCNGC 7 cut(s) 70, 198, 288, 1263, 1655, 1878, 2204
FspBI CTAG 4 cut(s) 6, 480, 1121, 1368
GlaI GCGC 2 cut(s) 546, 2365
GluI GCNGC 7 cut(s) 70, 198, 288, 1263, 1655, 1878, 2204
GsuI CTGGAG 2 cut(s) 300, 1162
HaeII RGCGCY 1 cut(s) 548
HaeIII GGCC 8 cut(s) 870, 1137, 1402, 1436, 1686, 1777, 1868, 2243
HapII CCGG 3 cut(s) 262, 832, 2460
HgaI GACGC 1 cut(s) 2224
HhaI GCGC 2 cut(s) 547, 2366
Hin1I GRCGYC 2 cut(s) 893, 1067
Hin6I GCGC 2 cut(s) 545, 2364
HinP1I GCGC 2 cut(s) 545, 2364
HincII GTYRAC 4 cut(s) 85, 1004, 1731, 2518
HindII GTYRAC 4 cut(s) 85, 1004, 1731, 2518
HindIII AAGCTT 2 cut(s) 847, 1844
HpaI GTTAAC 1 cut(s) 2518
HpaII CCGG 3 cut(s) 262, 832, 2460
HphI GGTGA 3 cut(s) 305, 611, 740
Hpy166II GTNNAC 7 cut(s) 85, 497, 720, 1004, 1731, 2422, 2518
Hpy8I GTNNAC 7 cut(s) 85, 497, 720, 1004, 1731, 2422, 2518
Hpy99I CGWCG 1 cut(s) 895
HpyAV CCTTC 6 cut(s) 40, 320, 364, 860, 980, 2273
HpyCH4IV ACGT 5 cut(s) 431, 716, 741, 893, 1067
HpyF3I CTNAG 7 cut(s) 357, 411, 583, 850, 1106, 1215, 1650
HpySE526I ACGT 5 cut(s) 431, 716, 741, 893, 1067
Hsp92I GRCGYC 2 cut(s) 893, 1067
HspAI GCGC 2 cut(s) 545, 2364
Ksp22I TGATCA 2 cut(s) 1321, 1831
KspAI GTTAAC 1 cut(s) 2518
LmnI GCTCC 7 cut(s) 62, 225, 1143, 1348, 1475, 1513, 2289
Lsp1109I GCAGC 7 cut(s) 56, 184, 274, 1274, 1666, 1889, 2190
LweI GCATC 8 cut(s) 274, 514, 863, 986, 1023, 1034, 1370, 1702
MaeI CTAG 4 cut(s) 6, 480, 1121, 1368
MaeII ACGT 5 cut(s) 431, 716, 741, 893, 1067
MaeIII GTNAC 6 cut(s) 188, 360, 376, 1090, 1782, 1954
MflI RGATCY 4 cut(s) 1184, 1289, 2169, 2342
MhlI GDGCHC 6 cut(s) 130, 582, 722, 1120, 1148, 2286
MlsI TGGCCA 3 cut(s) 1137, 1436, 1777
MluNI TGGCCA 3 cut(s) 1137, 1436, 1777
MlyI GAGTC 6 cut(s) 812, 1388, 1790, 2198, 2206, 2304
MmeI TCCRAC 4 cut(s) 19, 237, 1078, 2275
Mox20I TGGCCA 3 cut(s) 1137, 1436, 1777
Mph1103I ATGCAT 2 cut(s) 386, 979
MroXI GAANNNNTTC 2 cut(s) 918, 1704
MscI TGGCCA 3 cut(s) 1137, 1436, 1777
MseI TTAA 5 cut(s) 797, 1425, 2337, 2517, 2523
MslI CAYNNNNRTG 3 cut(s) 1176, 1416, 1806
Msp20I TGGCCA 3 cut(s) 1137, 1436, 1777
MspA1I CMGCKG 3 cut(s) 630, 1473, 1880
MspI CCGG 3 cut(s) 262, 832, 2460
MspR9I CCNGG 4 cut(s) 186, 567, 590, 2513
Mva1269I GAATGC 1 cut(s) 239
MvaI CCWGG 4 cut(s) 186, 567, 590, 2513
MvnI CGCG 1 cut(s) 2210
NcoI CCATGG 1 cut(s) 537
NdeI CATATG 2 cut(s) 782, 2102
NlaIV GGNNCC 7 cut(s) 127, 221, 1145, 1245, 1457, 2047, 2285
NmeAIII GCCGAG 1 cut(s) 1378
NmuCI GTSAC 4 cut(s) 188, 376, 1782, 1954
NruI TCGCGA 1 cut(s) 2210
NsiI ATGCAT 2 cut(s) 386, 979
NspI RCATGY 2 cut(s) 384, 2112
PaeI GCATGC 1 cut(s) 2112
PaeR7I CTCGAG 1 cut(s) 2149
PagI TCATGA 4 cut(s) 1318, 1417, 1834, 1975
PctI GAATGC 1 cut(s) 239
PdmI GAANNNNTTC 2 cut(s) 918, 1704
PfeI GAWTC 9 cut(s) 78, 512, 914, 1157, 1571, 2097, 2119, 2326, 2456
Pfl23II CGTACG 1 cut(s) 2383
PkrI GCNGC 7 cut(s) 71, 199, 289, 1264, 1656, 1879, 2205
PleI GAGTC 6 cut(s) 811, 1387, 1789, 2197, 2206, 2304
PpsI GAGTC 6 cut(s) 811, 1387, 1789, 2197, 2206, 2304
Ppu21I YACGTR 1 cut(s) 717
Psp6I CCWGG 4 cut(s) 184, 565, 588, 2511
PspGI CCWGG 4 cut(s) 184, 565, 588, 2511
PspLI CGTACG 1 cut(s) 2383
PspN4I GGNNCC 7 cut(s) 127, 221, 1145, 1245, 1457, 2047, 2285
PspPI GGNCC 4 cut(s) 259, 869, 1867, 2242
PspXI VCTCGAGB 1 cut(s) 2149
PsuI RGATCY 4 cut(s) 1184, 1289, 2169, 2342
RruI TCGCGA 1 cut(s) 2210
RsaI GTAC 7 cut(s) 314, 529, 574, 744, 876, 2132, 2385
RsaNI GTAC 7 cut(s) 313, 528, 573, 743, 875, 2131, 2384
RseI CAYNNNNRTG 3 cut(s) 1176, 1416, 1806
SaqAI TTAA 5 cut(s) 797, 1425, 2337, 2517, 2523
SatI GCNGC 7 cut(s) 70, 198, 288, 1263, 1655, 1878, 2204
Sau96I GGNCC 4 cut(s) 259, 869, 1867, 2242
ScaI AGTACT 2 cut(s) 314, 574
SchI GAGTC 6 cut(s) 812, 1388, 1790, 2198, 2206, 2304
ScrFI CCNGG 4 cut(s) 186, 567, 590, 2513
SduI GDGCHC 6 cut(s) 130, 582, 722, 1120, 1148, 2286
SfaNI GCATC 8 cut(s) 274, 514, 863, 986, 1023, 1034, 1370, 1702
Sfr274I CTCGAG 1 cut(s) 2149
SinI GGWCC 1 cut(s) 259
SlaI CTCGAG 1 cut(s) 2149
SmiMI CAYNNNNRTG 3 cut(s) 1176, 1416, 1806
SmlI CTYRAG 4 cut(s) 762, 1404, 2149, 2390
SmoI CTYRAG 4 cut(s) 762, 1404, 2149, 2390
SpeI ACTAGT 1 cut(s) 479
SphI GCATGC 1 cut(s) 2112
SsiI CCGC 5 cut(s) 9, 630, 1473, 1880, 2484
SspI AATATT 1 cut(s) 1164
SspMI CTAG 4 cut(s) 6, 480, 1121, 1368
StyD4I CCNGG 4 cut(s) 184, 565, 588, 2511
StyI CCWWGG 3 cut(s) 537, 1346, 1444
TaiI ACGT 5 cut(s) 434, 719, 744, 896, 1070
TaqI TCGA 7 cut(s) 19, 655, 1333, 1462, 1689, 2034, 2150
TaqII GACCGA 1 cut(s) 2246
TatI WGTACW 3 cut(s) 312, 572, 874
TfiI GAWTC 9 cut(s) 78, 512, 914, 1157, 1571, 2097, 2119, 2326, 2456
Tru1I TTAA 5 cut(s) 797, 1425, 2337, 2517, 2523
Tru9I TTAA 5 cut(s) 797, 1425, 2337, 2517, 2523
TscAI CASTG 8 cut(s) 221, 957, 1145, 1219, 1834, 1959, 2169, 2202
TseFI GTSAC 4 cut(s) 188, 376, 1782, 1954
TseI GCWGC 7 cut(s) 69, 197, 287, 1262, 1654, 1877, 2203
Tsp45I GTSAC 4 cut(s) 188, 376, 1782, 1954
TspGWI ACGGA 1 cut(s) 2322
TspRI CASTG 8 cut(s) 221, 957, 1145, 1219, 1834, 1959, 2169, 2202
VneI GTGCAC 1 cut(s) 718
VpaK11BI GGWCC 1 cut(s) 259
XapI RAATTY 6 cut(s) 168, 615, 1335, 1967, 1985, 2135
XceI RCATGY 2 cut(s) 384, 2112
XhoI CTCGAG 1 cut(s) 2149
XmnI GAANNNNTTC 2 cut(s) 918, 1704
XspI CTAG 4 cut(s) 6, 480, 1121, 1368
ZraI GACGTC 2 cut(s) 894, 1068
ZrmI AGTACT 2 cut(s) 314, 574
Zsp2I ATGCAT 2 cut(s) 386, 979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.