MD16G1040000.v1.1

Chaperone protein DNAj

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013340
Physical Location & Seq
Forward (+)
2835042 .. 2839885
4844 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1040000.v1.1.491

Sequence Viewer

Length: 1248 bp
ATGGGGACGAATTCGAAATTGGAAGGGACGTCGGCGCCGGCGATACGGCGGGACCCCTATGAGGTGCTGTGCGTTTCGAGGGATTCGACTGATCAGGAGATTAAAACCGCTTACAGAAAGCTTGCTCTCAAGTATCATCCAGACAAGAATACCAACAATCCTGAAGCTTCAGAACATTTCAAGGAGGTTGCATTTTCGTATAGCATCTTGTCGGATCCAGAGAAAAGGAGGCAGTACGACAATGCAGGGTTTGAGGCTCTTGATGCGGATGCCATGGACATGGAAATTGACTTGTCAAACCTGGGGACTGTCAATACAATGTTTGCTGCTTTATTCAGCAAGTTGGGTGTCCCTATCAAGACTACAATTTCTGCCAATGTACTTGAAGAAGCTCTTAATGGTACAGTTACAGTCAGACCTCTCCCAATTGGAACTTCAGTTAGTGGAAAGGTGGAAAAACAGTGTGCTCACTTTTTTGGTGTAACAATCAGTGAGCAACAAGCCGAATCGGGGATTGTAGTTAGAGTTACCTCAACTGCACAAAGCAAATTTAAGTTACTTTATTTCGAGCAAGATGTCCATGGTGGTTATGGTTTAGCCTTACAGGAAGATAGTGAGAAGACAGGCAAAATAACGTCTGCAGGGATGTATTTTCTGCATTTTCAAGTATACAGAATGGATTCGACTTTGAATGCGTTGGCTATGGCAAAGGACCCTGAAGCTGCTTTCTTTAAGAGGTTGGAAGGTCTTCAACCTTGTGAGGTTTCAGAACTAAAAGCTGGCACTCATATATTCGCTGTTTATGGAGACAATTTTTTTAAGACTGCTACCTATACAATTGAGGCACTTTGTGTAAGGTCATACGAGGATACGACGGAGAAGCTTAAAGAGATTGAGGCTCAGATTTTAAGAAAGAGAAATGAACTTCGTCAATTTGAGACAGAGTACAGGAAGGCATTGCAACGCTTTCAAGAAGTGACCAACAGATACACCCAAGAAAAGCAATCTGTAGATGAGCTGCTGAAACAGAGAGACCATATCCATTCTTCTTTCACTATCACCAGGACAAGCAGTATCATCGTGGGTGGTGGTAGTGGTATGGCTAACGGCAGCACTAGCAAAAATCCTGGCGAGGATTCAAAGGCGGAGAGCCCAGTGGAAGATGGAGGCTCTGACGGGAAGGATAAGTCGGGTAAAAAGAAATGGTTCAATCTTAACCTCAAAGGATCAGATAAAAAACTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008092 GO:0008150 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009958 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

416

Amino Acids

46.11

Weight (kDa)

6.14

Isoelectric Point (pI)

34.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 18 - 80 4.3e-27 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013340)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68370
fragaria_vesca FvH4_4g27000
malus_domestica MD13G1039100.v1.1 MD16G1040000.v1.1
prunus_persica Prupe.1G314100_v2.0.a1 Prupe.1G314100_v2.0.a1 Prupe.1G314100_v2.0.a1
pyrus_communis pycom13g03360
rosa_chinensis RchiOBHm_Chr4g0434801
rosa_laevigata RLG00000006648
rosa_multiflora Rmu_sc0000179.1_g000002
rosa_roxburghii Rroxscaffold_5G00376010
rosa_rugosa Rorug04G0278500
rosa_samantha Rh4AG335000 Rh4BG343500 Rh4CG357900 Rh4DG337400
rosa_wichuraiana Rw4G029210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 32
AccB1I GGYRCC 1 cut(s) 34
AccI GTMKAC 1 cut(s) 669
AciI CCGC 4 cut(s) 49, 108, 266, 1145
AclWI GGATC 3 cut(s) 209, 222, 1234
AcsI RAATTY 2 cut(s) 10, 548
AcuI CTGAAG 4 cut(s) 153, 183, 420, 738
AcyI GRCGYC 2 cut(s) 29, 35
AdeI CACNNNGTG 1 cut(s) 851
AfaI GTAC 4 cut(s) 236, 381, 403, 947
AfiI CCNNNNNNNGG 2 cut(s) 61, 510
AgsI TTSAA 8 cut(s) 181, 386, 665, 691, 752, 971, 1140, 1210
AjnI CCWGG 3 cut(s) 300, 1061, 1126
AjuI GAANNNNNNNTTGG 3 cut(s) 34, 418, 450
AluBI AGCT 7 cut(s) 121, 167, 392, 722, 779, 883, 1018
AluI AGCT 7 cut(s) 121, 167, 392, 722, 779, 883, 1018
Alw21I GWGCWC 1 cut(s) 469
Alw26I GTCTC 3 cut(s) 801, 932, 1026
AlwI GGATC 3 cut(s) 209, 222, 1234
ApeKI GCWGC 4 cut(s) 326, 722, 1018, 1110
ApoI RAATTY 2 cut(s) 10, 548
Asp700I GAANNNNTTC 3 cut(s) 679, 747, 1205
AspLEI GCGC 1 cut(s) 37
AspS9I GGNCC 2 cut(s) 52, 712
AsuHPI GGTGA 1 cut(s) 1051
AsuII TTCGAA 1 cut(s) 14
AvaII GGWCC 2 cut(s) 52, 712
BamHI GGATCC 1 cut(s) 214
BanI GGYRCC 1 cut(s) 34
BanII GRGCYC 1 cut(s) 1154
BbsI GAAGAC 2 cut(s) 626, 740
Bbv12I GWGCWC 1 cut(s) 469
BbvI GCAGC 4 cut(s) 313, 709, 1005, 1122
BccI CCATC 1 cut(s) 1157
BceAI ACGGC 2 cut(s) 62, 1123
BcgI CGANNNNNNTGC 2 cut(s) 1060, 1094
BciT130I CCWGG 3 cut(s) 302, 1063, 1128
BciVI GTATCC 1 cut(s) 862
BclI TGATCA 1 cut(s) 91
BcoDI GTCTC 3 cut(s) 801, 932, 1026
BfaI CTAG 1 cut(s) 1116
BfmI CTRYAG 2 cut(s) 639, 1008
BfoI RGCGCY 1 cut(s) 38
BfuI GTATCC 1 cut(s) 862
BisI GCNGC 4 cut(s) 327, 723, 1019, 1111
BlsI GCNGC 4 cut(s) 328, 724, 1020, 1112
Bme1390I CCNGG 3 cut(s) 302, 1063, 1128
Bme18I GGWCC 2 cut(s) 52, 712
BmgT120I GGNCC 2 cut(s) 52, 712
BmiI GGNNCC 5 cut(s) 36, 53, 54, 216, 714
BmrFI CCNGG 3 cut(s) 302, 1063, 1128
BmrI ACTGGG 1 cut(s) 1148
BmsI GCATC 3 cut(s) 213, 253, 259
BmuI ACTGGG 1 cut(s) 1148
BpiI GAAGAC 2 cut(s) 626, 740
Bpu14I TTCGAA 1 cut(s) 14
BpuEI CTTGAG 1 cut(s) 113
BsaHI GRCGYC 2 cut(s) 29, 35
BsaI GGTCTC 1 cut(s) 1026
BsaJI CCNNGG 3 cut(s) 273, 301, 580
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 510
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 1 cut(s) 1154
Bse3DI GCAATG 1 cut(s) 956
BseBI CCWGG 3 cut(s) 302, 1063, 1128
BseDI CCNNGG 3 cut(s) 273, 301, 580
BseGI GGATG 3 cut(s) 136, 274, 651
BseLI CCNNNNNNNGG 2 cut(s) 61, 510
BseMI GCAATG 1 cut(s) 956
BseMII CTCAG 1 cut(s) 914
BseNI ACTGG 1 cut(s) 1154
BseXI GCAGC 4 cut(s) 313, 709, 1005, 1122
BsgI GTGCAG 1 cut(s) 522
BshNI GGYRCC 1 cut(s) 34
BsiHKAI GWGCWC 1 cut(s) 469
BsiSI CCGG 1 cut(s) 38
BslFI GGGAC 5 cut(s) 19, 40, 65, 319, 335
BslI CCNNNNNNNGG 2 cut(s) 61, 510
BsmAI GTCTC 3 cut(s) 801, 932, 1026
BsmFI GGGAC 5 cut(s) 19, 40, 65, 319, 335
BsmI GAATGC 1 cut(s) 697
Bso31I GGTCTC 1 cut(s) 1026
Bsp119I TTCGAA 1 cut(s) 14
Bsp1286I GDGCHC 2 cut(s) 469, 1154
Bsp143I GATC 3 cut(s) 91, 214, 1226
Bsp19I CCATGG 2 cut(s) 273, 580
BspACI CCGC 4 cut(s) 49, 108, 266, 1145
BspCNI CTCAG 1 cut(s) 913
BspLI GGNNCC 5 cut(s) 36, 53, 54, 216, 714
BspMAI CTGCAG 1 cut(s) 643
BspPI GGATC 3 cut(s) 209, 222, 1234
BspT104I TTCGAA 1 cut(s) 14
BspT107I GGYRCC 1 cut(s) 34
BspTNI GGTCTC 1 cut(s) 1026
BsrDI GCAATG 1 cut(s) 956
BsrFI RCCGGY 1 cut(s) 37
BsrI ACTGG 1 cut(s) 1154
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 3 cut(s) 273, 301, 580
BssMI GATC 3 cut(s) 91, 214, 1226
BssNAI GTATAC 1 cut(s) 670
BssNI GRCGYC 2 cut(s) 29, 35
BssT1I CCWWGG 2 cut(s) 273, 580
Bst1107I GTATAC 1 cut(s) 670
Bst2UI CCWGG 3 cut(s) 302, 1063, 1128
Bst4CI ACNGT 4 cut(s) 310, 406, 412, 462
BstACI GRCGYC 2 cut(s) 29, 35
BstBI TTCGAA 1 cut(s) 14
BstC8I GCNNGC 3 cut(s) 39, 123, 781
BstDEI CTNAG 1 cut(s) 900
BstDSI CCRYGG 2 cut(s) 273, 580
BstF5I GGATG 3 cut(s) 136, 274, 651
BstH2I RGCGCY 1 cut(s) 38
BstHHI GCGC 1 cut(s) 37
BstKTI GATC 3 cut(s) 94, 217, 1229
BstMAI GTCTC 3 cut(s) 801, 932, 1026
BstMBI GATC 3 cut(s) 91, 214, 1226
BstMWI GCNNNNNNNGC 2 cut(s) 263, 1116
BstNI CCWGG 3 cut(s) 302, 1063, 1128
BstSCI CCNGG 3 cut(s) 300, 1061, 1126
BstSFI CTRYAG 2 cut(s) 639, 1008
BstV1I GCAGC 4 cut(s) 313, 709, 1005, 1122
BstV2I GAAGAC 2 cut(s) 626, 740
BstX2I RGATCY 1 cut(s) 214
BstXI CCANNNNNNTGG 1 cut(s) 280
BstYI RGATCY 1 cut(s) 214
BstZ17I GTATAC 1 cut(s) 670
BsuI GTATCC 1 cut(s) 862
BtgI CCRYGG 2 cut(s) 273, 580
BtsCI GGATG 3 cut(s) 136, 274, 651
BtsIMutI CAGTG 3 cut(s) 467, 496, 1161
Cac8I GCNNGC 3 cut(s) 39, 123, 781
CfoI GCGC 1 cut(s) 37
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 2 cut(s) 52, 712
Csp6I GTAC 4 cut(s) 235, 380, 402, 946
CviAII CATG 3 cut(s) 274, 280, 581
CviQI GTAC 4 cut(s) 235, 380, 402, 946
DdeI CTNAG 1 cut(s) 900
DinI GGCGCC 1 cut(s) 36
DpnI GATC 3 cut(s) 93, 216, 1228
DpnII GATC 3 cut(s) 91, 214, 1226
DraIII CACNNNGTG 1 cut(s) 851
EciI GGCGGA 1 cut(s) 1160
Eco130I CCWWGG 2 cut(s) 273, 580
Eco24I GRGCYC 1 cut(s) 1154
Eco31I GGTCTC 1 cut(s) 1026
Eco47I GGWCC 2 cut(s) 52, 712
Eco57I CTGAAG 4 cut(s) 153, 183, 420, 738
EcoO109I RGGNCCY 2 cut(s) 52, 712
EcoRI GAATTC 1 cut(s) 10
EcoRII CCWGG 3 cut(s) 300, 1061, 1126
EcoT14I CCWWGG 2 cut(s) 273, 580
EcoT38I GRGCYC 1 cut(s) 1154
EgeI GGCGCC 1 cut(s) 36
EheI GGCGCC 1 cut(s) 36
ErhI CCWWGG 2 cut(s) 273, 580
FaeI CATG 3 cut(s) 277, 283, 584
FalI AAGNNNNNCTT 2 cut(s) 378, 410
FaqI GGGAC 5 cut(s) 19, 40, 65, 319, 335
FatI CATG 3 cut(s) 273, 279, 580
FauI CCCGC 1 cut(s) 42
FbaI TGATCA 1 cut(s) 91
FblI GTMKAC 1 cut(s) 669
Fnu4HI GCNGC 4 cut(s) 327, 723, 1019, 1111
FokI GGATG 3 cut(s) 123, 281, 658
FriOI GRGCYC 1 cut(s) 1154
Fsp4HI GCNGC 4 cut(s) 327, 723, 1019, 1111
FspBI CTAG 1 cut(s) 1116
GlaI GCGC 1 cut(s) 36
GluI GCNGC 4 cut(s) 327, 723, 1019, 1111
HaeII RGCGCY 1 cut(s) 38
HapII CCGG 1 cut(s) 38
HhaI GCGC 1 cut(s) 37
Hin1I GRCGYC 2 cut(s) 29, 35
Hin1II CATG 3 cut(s) 277, 283, 584
Hin6I GCGC 1 cut(s) 35
HinP1I GCGC 1 cut(s) 35
HindIII AAGCTT 3 cut(s) 119, 165, 881
HinfI GANTC 4 cut(s) 83, 506, 680, 1136
HpaII CCGG 1 cut(s) 38
HphI GGTGA 1 cut(s) 1051
Hpy166II GTNNAC 1 cut(s) 670
Hpy188I TCNGA 7 cut(s) 172, 214, 416, 769, 903, 1174, 1231
Hpy188III TCNNGA 7 cut(s) 95, 140, 161, 218, 260, 358, 971
Hpy8I GTNNAC 1 cut(s) 670
Hpy99I CGWCG 2 cut(s) 34, 877
HpyAV CCTTC 4 cut(s) 17, 737, 946, 1174
HpyCH4III ACNGT 4 cut(s) 310, 406, 412, 462
HpyCH4IV ACGT 2 cut(s) 29, 635
HpyCH4V TGCA 6 cut(s) 191, 245, 539, 641, 658, 961
HpyF10VI GCNNNNNNNGC 2 cut(s) 263, 1116
HpyF3I CTNAG 1 cut(s) 900
HpySE526I ACGT 2 cut(s) 29, 635
Hsp92I GRCGYC 2 cut(s) 29, 35
Hsp92II CATG 3 cut(s) 277, 283, 584
HspAI GCGC 1 cut(s) 35
KasI GGCGCC 1 cut(s) 34
KflI GGGWCCC 1 cut(s) 52
KroI GCCGGC 1 cut(s) 37
KroNI GCCGGC 1 cut(s) 39
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 3 cut(s) 91, 214, 1226
Lsp1109I GCAGC 4 cut(s) 313, 709, 1005, 1122
LweI GCATC 3 cut(s) 213, 253, 259
MaeI CTAG 1 cut(s) 1116
MaeII ACGT 2 cut(s) 29, 635
MaeIII GTNAC 5 cut(s) 406, 481, 526, 555, 976
MalI GATC 3 cut(s) 93, 216, 1228
MboI GATC 3 cut(s) 91, 214, 1226
MboII GAAGA 6 cut(s) 398, 620, 631, 740, 1038, 1172
MfeI CAATTG 2 cut(s) 426, 837
MflI RGATCY 1 cut(s) 214
MhlI GDGCHC 2 cut(s) 469, 1154
MluCI AATT 9 cut(s) 10, 17, 285, 366, 426, 548, 811, 837, 932
Mly113I GGCGCC 1 cut(s) 35
MmeI TCCRAC 2 cut(s) 192, 720
MreI CGCCGGCG 1 cut(s) 37
MroNI GCCGGC 1 cut(s) 37
MroXI GAANNNNTTC 3 cut(s) 679, 747, 1205
MseI TTAA 8 cut(s) 102, 396, 552, 732, 819, 885, 908, 1215
MslI CAYNNNNRTG 1 cut(s) 278
MspI CCGG 1 cut(s) 38
MspR9I CCNGG 3 cut(s) 302, 1063, 1128
MunI CAATTG 2 cut(s) 426, 837
Mva1269I GAATGC 1 cut(s) 697
MvaI CCWGG 3 cut(s) 302, 1063, 1128
MwoI GCNNNNNNNGC 2 cut(s) 263, 1116
NaeI GCCGGC 1 cut(s) 39
NarI GGCGCC 1 cut(s) 35
NcoI CCATGG 2 cut(s) 273, 580
NdeII GATC 3 cut(s) 91, 214, 1226
NgoMIV GCCGGC 1 cut(s) 37
NlaIII CATG 3 cut(s) 277, 283, 584
NlaIV GGNNCC 5 cut(s) 36, 53, 54, 216, 714
NmuCI GTSAC 1 cut(s) 976
NspV TTCGAA 1 cut(s) 14
PcsI WCGNNNNNNNCGW 2 cut(s) 38, 83
PctI GAATGC 1 cut(s) 697
PdiI GCCGGC 1 cut(s) 39
PdmI GAANNNNTTC 3 cut(s) 679, 747, 1205
PfeI GAWTC 4 cut(s) 83, 506, 680, 1136
PkrI GCNGC 4 cut(s) 328, 724, 1020, 1112
PluTI GGCGCC 1 cut(s) 38
PpuMI RGGWCCY 2 cut(s) 52, 712
Psp5II RGGWCCY 2 cut(s) 52, 712
Psp6I CCWGG 3 cut(s) 300, 1061, 1126
PspGI CCWGG 3 cut(s) 300, 1061, 1126
PspN4I GGNNCC 5 cut(s) 36, 53, 54, 216, 714
PspPI GGNCC 2 cut(s) 52, 712
PspPPI RGGWCCY 2 cut(s) 52, 712
PstI CTGCAG 1 cut(s) 643
PsuI RGATCY 1 cut(s) 214
RsaI GTAC 4 cut(s) 236, 381, 403, 947
RsaNI GTAC 4 cut(s) 235, 380, 402, 946
RseI CAYNNNNRTG 1 cut(s) 278
SaqAI TTAA 8 cut(s) 102, 396, 552, 732, 819, 885, 908, 1215
SatI GCNGC 4 cut(s) 327, 723, 1019, 1111
Sau3AI GATC 3 cut(s) 91, 214, 1226
Sau96I GGNCC 2 cut(s) 52, 712
ScrFI CCNGG 3 cut(s) 302, 1063, 1128
SduI GDGCHC 2 cut(s) 469, 1154
SfaNI GCATC 3 cut(s) 213, 253, 259
SfcI CTRYAG 2 cut(s) 639, 1008
SfoI GGCGCC 1 cut(s) 36
SfuI TTCGAA 1 cut(s) 14
SgrAI CRCCGGYG 1 cut(s) 37
SinI GGWCC 2 cut(s) 52, 712
SmiMI CAYNNNNRTG 1 cut(s) 278
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse9I AATT 9 cut(s) 10, 17, 285, 366, 426, 548, 811, 837, 932
SsiI CCGC 4 cut(s) 49, 108, 266, 1145
SspDI GGCGCC 1 cut(s) 34
SspMI CTAG 1 cut(s) 1116
StyD4I CCNGG 3 cut(s) 300, 1061, 1126
StyI CCWWGG 2 cut(s) 273, 580
TaaI ACNGT 4 cut(s) 310, 406, 412, 462
TaiI ACGT 2 cut(s) 32, 638
TaqI TCGA 5 cut(s) 14, 77, 86, 567, 683
TasI AATT 9 cut(s) 10, 17, 285, 366, 426, 548, 811, 837, 932
TatI WGTACW 2 cut(s) 379, 945
TfiI GAWTC 4 cut(s) 83, 506, 680, 1136
Tru1I TTAA 8 cut(s) 102, 396, 552, 732, 819, 885, 908, 1215
Tru9I TTAA 8 cut(s) 102, 396, 552, 732, 819, 885, 908, 1215
TscAI CASTG 3 cut(s) 467, 496, 1161
TseFI GTSAC 1 cut(s) 976
TseI GCWGC 4 cut(s) 326, 722, 1018, 1110
Tsp45I GTSAC 1 cut(s) 976
TspDTI ATGAA 1 cut(s) 936
TspGWI ACGGA 1 cut(s) 890
TspRI CASTG 3 cut(s) 467, 496, 1161
VpaK11BI GGWCC 2 cut(s) 52, 712
XapI RAATTY 2 cut(s) 10, 548
XcmI CCANNNNNNNNNTGG 1 cut(s) 587
XmiI GTMKAC 1 cut(s) 669
XmnI GAANNNNTTC 3 cut(s) 679, 747, 1205
XspI CTAG 1 cut(s) 1116
ZraI GACGTC 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.