MD16G1040500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0016536
Physical Location & Seq
Reverse (-)
2873156 .. 2874022
867 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1040500.v1.1.491

Sequence Viewer

Length: 867 bp
ATGGCCATTGAGGTATGCTCAGAGATTTCGAGCCCCGGATTCAGTCCAAGAATTTCATTCTCCCATGATCTGGATAAGAAGAATCATGACTCGAACCAAGGGCAACGGTTAGACTCCTCTCTCTTGGATTCCACTTCTGATTTCGACTTCTGCATTGTAAACAATCTCAAGCTGGAGTTGTCTTCGGCGGACGAGCTTTTCTCCAATGGGAAGATCCTCCCTGTCCAAATCAAGAGAAATCTCAACGCTGCCGAAGACACCCACCAGCCTGACGTTACTCGCCAGGCTCGTGAGAGGGCAGTTCCTCCAGCCCTTGCTCATCACTCAAAACCTCTAAATACAGAGAGGCTGAAAACAGAGGAGAAGAAGAAGAGGTTGAAAGAATTTCTAGACACCAACGCCGATGCAGATGAAACCGACAACCCTCCAACAGCAAAGCCATTCTGGCAGTTCCGGCGGAGCAGCAGCCTCAACTGCGACGCTGCCCGCGGCAAGAGCCTAATCCGCCCGCTGCAGTTTCTTTCGAGAAGCAACTCAACAGGGTCGGCTCCAAACCCAAATCAAAAGCAAGCATTGCCTCCAAAGGAAAGAACAACAACAATTCATCAGAAGCAGCAGCATTTGCGGAAACAGACGTCGGTTTCGAGTAGAAGATCGTCGTCTGCGTCCTCGTGCTCGAGTACATATTATCCTTACAACAACATGAATCAAAAGCCTGCGTTGAGAAGGAGTGGATCTTACGGCAATGGGGTTAGAAACAACCCTGTCCTGAACCTCCCACCGCCATACATTTCCAAAGTTACCGTGAGTTTTTTCGGGTTCGGTTCTCTCTTCTGTAGTGGTAAATTTAGAAAGAAGAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

289

Amino Acids

32.27

Weight (kDa)

9.88

Isoelectric Point (pI)

53.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016536)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68330
fragaria_vesca FvH4_4g27060
malus_domestica MD13G1039600.v1.1 MD16G1040500.v1.1
prunus_persica Prupe.1G313600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0434861
rosa_laevigata RLG00000006640
rosa_multiflora Rmu_sc0003726.1_g000002
rosa_roxburghii Rroxscaffold_5G00376070
rosa_rugosa Rorug04G0279700
rosa_samantha Rh4AG335500 Rh4BG344000 Rh4DG338000
rosa_wichuraiana Rw4G029270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 638
AccB7I CCANNNNNTGG 1 cut(s) 70
AccII CGCG 1 cut(s) 489
AciI CCGC 8 cut(s) 188, 457, 487, 489, 505, 509, 625, 782
AclWI GGATC 2 cut(s) 208, 742
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 51, 383, 845
AcyI GRCGYC 1 cut(s) 635
AfaI GTAC 1 cut(s) 682
AfiI CCNNNNNNNGG 1 cut(s) 70
AgsI TTSAA 1 cut(s) 379
AjnI CCWGG 1 cut(s) 282
AluBI AGCT 2 cut(s) 172, 196
AluI AGCT 2 cut(s) 172, 196
Alw21I GWGCWC 1 cut(s) 677
AlwI GGATC 2 cut(s) 208, 742
Ama87I CYCGRG 1 cut(s) 676
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 7 cut(s) 248, 462, 465, 482, 511, 613, 616
ApoI RAATTY 3 cut(s) 51, 383, 845
ArsI GACNNNNNNTTYG 2 cut(s) 625, 657
AsuC2I CCSGG 1 cut(s) 36
AvaI CYCGRG 1 cut(s) 676
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 35
BauI CACGAG 2 cut(s) 288, 670
BbsI GAAGAC 2 cut(s) 174, 261
Bbv12I GWGCWC 1 cut(s) 677
BbvI GCAGC 7 cut(s) 235, 469, 474, 477, 498, 625, 628
BceAI ACGGC 1 cut(s) 757
BciT130I CCWGG 1 cut(s) 284
BcnI CCSGG 1 cut(s) 36
BfaI CTAG 1 cut(s) 389
BfmI CTRYAG 2 cut(s) 512, 835
BglI GCCNNNNNGGC 1 cut(s) 445
BisI GCNGC 8 cut(s) 249, 463, 466, 483, 490, 512, 614, 617
BlsI GCNGC 8 cut(s) 250, 464, 467, 484, 491, 513, 615, 618
Bme1390I CCNGG 2 cut(s) 36, 284
BmeT110I CYCGRG 1 cut(s) 676
BmiI GGNNCC 1 cut(s) 549
BmrFI CCNGG 2 cut(s) 36, 284
BmsI GCATC 1 cut(s) 394
BpiI GAAGAC 2 cut(s) 174, 261
BplI GAGNNNNNCTC 3 cut(s) 34, 185, 217
BpmI CTGGAG 2 cut(s) 194, 291
BpuEI CTTGAG 1 cut(s) 152
BpuMI CCSGG 1 cut(s) 36
BsaHI GRCGYC 1 cut(s) 635
BsaJI CCNNGG 3 cut(s) 34, 97, 487
Bsc4I CCNNNNNNNGG 1 cut(s) 70
Bse3DI GCAATG 2 cut(s) 572, 751
BseBI CCWGG 1 cut(s) 284
BseDI CCNNGG 3 cut(s) 34, 97, 487
BseLI CCNNNNNNNGG 1 cut(s) 70
BseMI GCAATG 2 cut(s) 572, 751
BseMII CTCAG 1 cut(s) 33
BseRI GAGGAG 2 cut(s) 106, 374
BseXI GCAGC 7 cut(s) 235, 469, 474, 477, 498, 625, 628
Bsh1236I CGCG 1 cut(s) 489
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 677
BsiHKCI CYCGRG 1 cut(s) 676
BsiSI CCGG 2 cut(s) 36, 454
BslI CCNNNNNNNGG 1 cut(s) 70
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 676
Bsp1286I GDGCHC 2 cut(s) 35, 677
Bsp143I GATC 4 cut(s) 67, 213, 653, 734
BspACI CCGC 8 cut(s) 188, 457, 487, 489, 505, 509, 625, 782
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 32
BspFNI CGCG 1 cut(s) 489
BspHI TCATGA 1 cut(s) 85
BspLI GGNNCC 1 cut(s) 549
BspMAI CTGCAG 1 cut(s) 516
BspPI GGATC 2 cut(s) 208, 742
BsrDI GCAATG 2 cut(s) 572, 751
BssECI CCNNGG 3 cut(s) 34, 97, 487
BssMI GATC 4 cut(s) 67, 213, 653, 734
BssNI GRCGYC 1 cut(s) 635
BssSI CACGAG 2 cut(s) 288, 670
BssT1I CCWWGG 1 cut(s) 97
Bst2BI CACGAG 2 cut(s) 288, 670
Bst2UI CCWGG 1 cut(s) 284
Bst4CI ACNGT 2 cut(s) 108, 805
Bst6I CTCTTC 2 cut(s) 365, 836
BstACI GRCGYC 1 cut(s) 635
BstAPI GCANNNNNTGC 2 cut(s) 574, 622
BstC8I GCNNGC 4 cut(s) 487, 509, 570, 717
BstDEI CTNAG 1 cut(s) 19
BstDSI CCRYGG 1 cut(s) 487
BstFNI CGCG 1 cut(s) 489
BstKTI GATC 4 cut(s) 70, 216, 656, 737
BstMBI GATC 4 cut(s) 67, 213, 653, 734
BstMWI GCNNNNNNNGC 7 cut(s) 445, 454, 474, 495, 504, 574, 622
BstNI CCWGG 1 cut(s) 284
BstSCI CCNGG 2 cut(s) 34, 282
BstSFI CTRYAG 2 cut(s) 512, 835
BstUI CGCG 1 cut(s) 489
BstV1I GCAGC 7 cut(s) 235, 469, 474, 477, 498, 625, 628
BstV2I GAAGAC 2 cut(s) 174, 261
BstX2I RGATCY 2 cut(s) 213, 734
BstYI RGATCY 2 cut(s) 213, 734
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 487
Cac8I GCNNGC 4 cut(s) 487, 509, 570, 717
CciI TCATGA 1 cut(s) 85
Cfr42I CCGCGG 1 cut(s) 490
CseI GACGC 2 cut(s) 488, 654
Csp6I GTAC 1 cut(s) 681
CviAII CATG 3 cut(s) 65, 86, 703
CviQI GTAC 1 cut(s) 681
DdeI CTNAG 1 cut(s) 19
DpnI GATC 4 cut(s) 69, 215, 655, 736
DpnII GATC 4 cut(s) 67, 213, 653, 734
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 365, 836
EarI CTCTTC 2 cut(s) 365, 836
EciI GGCGGA 3 cut(s) 203, 472, 494
Eco130I CCWWGG 1 cut(s) 97
Eco24I GRGCYC 1 cut(s) 35
Eco88I CYCGRG 1 cut(s) 676
EcoRII CCWGG 1 cut(s) 282
EcoT14I CCWWGG 1 cut(s) 97
EcoT38I GRGCYC 1 cut(s) 35
ErhI CCWWGG 1 cut(s) 97
FaeI CATG 3 cut(s) 68, 89, 706
FaiI YATR 6 cut(s) 16, 66, 87, 685, 704, 787
FatI CATG 3 cut(s) 64, 85, 702
FauI CCCGC 2 cut(s) 494, 516
Fnu4HI GCNGC 8 cut(s) 249, 463, 466, 483, 490, 512, 614, 617
FriOI GRGCYC 1 cut(s) 35
Fsp4HI GCNGC 8 cut(s) 249, 463, 466, 483, 490, 512, 614, 617
FspBI CTAG 1 cut(s) 389
GluI GCNGC 8 cut(s) 249, 463, 466, 483, 490, 512, 614, 617
GsuI CTGGAG 2 cut(s) 194, 291
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 36, 454
HgaI GACGC 2 cut(s) 488, 654
Hin1I GRCGYC 1 cut(s) 635
Hin1II CATG 3 cut(s) 68, 89, 706
HinfI GANTC 6 cut(s) 39, 82, 89, 113, 128, 706
HpaII CCGG 2 cut(s) 36, 454
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 3 cut(s) 22, 139, 609
Hpy188III TCNNGA 7 cut(s) 71, 86, 232, 290, 389, 525, 769
Hpy8I GTNNAC 1 cut(s) 160
Hpy99I CGWCG 3 cut(s) 482, 640, 661
HpyAV CCTTC 1 cut(s) 720
HpyCH4III ACNGT 2 cut(s) 108, 805
HpyCH4IV ACGT 2 cut(s) 273, 635
HpyCH4V TGCA 3 cut(s) 153, 407, 514
HpyF10VI GCNNNNNNNGC 7 cut(s) 445, 454, 474, 495, 504, 574, 622
HpyF3I CTNAG 1 cut(s) 19
HpySE526I ACGT 2 cut(s) 273, 635
Hsp92I GRCGYC 1 cut(s) 635
Hsp92II CATG 3 cut(s) 68, 89, 706
KspI CCGCGG 1 cut(s) 490
Kzo9I GATC 4 cut(s) 67, 213, 653, 734
LmnI GCTCC 2 cut(s) 459, 553
Lsp1109I GCAGC 7 cut(s) 235, 469, 474, 477, 498, 625, 628
LweI GCATC 1 cut(s) 394
MaeI CTAG 1 cut(s) 389
MaeII ACGT 2 cut(s) 273, 635
MaeIII GTNAC 2 cut(s) 274, 799
MalI GATC 4 cut(s) 69, 215, 655, 736
MboI GATC 4 cut(s) 67, 213, 653, 734
MboII GAAGA 9 cut(s) 91, 174, 223, 266, 376, 379, 382, 663, 823
MflI RGATCY 2 cut(s) 213, 734
MhlI GDGCHC 2 cut(s) 35, 677
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 51, 383, 600, 845
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 83, 107
MmeI TCCRAC 1 cut(s) 452
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 489, 511
MspI CCGG 2 cut(s) 36, 454
MspR9I CCNGG 2 cut(s) 36, 284
MvaI CCWGG 1 cut(s) 284
MvnI CGCG 1 cut(s) 489
MwoI GCNNNNNNNGC 7 cut(s) 445, 454, 474, 495, 504, 574, 622
NciI CCSGG 1 cut(s) 36
NdeII GATC 4 cut(s) 67, 213, 653, 734
NlaIII CATG 3 cut(s) 68, 89, 706
NlaIV GGNNCC 1 cut(s) 549
PaeR7I CTCGAG 1 cut(s) 676
PagI TCATGA 1 cut(s) 85
PcsI WCGNNNNNNNCGW 3 cut(s) 286, 641, 662
PfeI GAWTC 4 cut(s) 39, 82, 128, 706
PflMI CCANNNNNTGG 1 cut(s) 70
PkrI GCNGC 8 cut(s) 250, 464, 467, 484, 491, 513, 615, 618
PleI GAGTC 2 cut(s) 83, 107
PpsI GAGTC 2 cut(s) 83, 107
Psp6I CCWGG 1 cut(s) 282
PspGI CCWGG 1 cut(s) 282
PspN4I GGNNCC 1 cut(s) 549
PspXI VCTCGAGB 1 cut(s) 676
PstI CTGCAG 1 cut(s) 516
PsuI RGATCY 2 cut(s) 213, 734
RsaI GTAC 1 cut(s) 682
RsaNI GTAC 1 cut(s) 681
SacII CCGCGG 1 cut(s) 490
SatI GCNGC 8 cut(s) 249, 463, 466, 483, 490, 512, 614, 617
Sau3AI GATC 4 cut(s) 67, 213, 653, 734
SchI GAGTC 2 cut(s) 83, 107
ScrFI CCNGG 2 cut(s) 36, 284
SduI GDGCHC 2 cut(s) 35, 677
SetI ASST 8 cut(s) 15, 174, 198, 276, 334, 377, 638, 777
SfaNI GCATC 1 cut(s) 394
SfcI CTRYAG 2 cut(s) 512, 835
Sfr274I CTCGAG 1 cut(s) 676
Sfr303I CCGCGG 1 cut(s) 490
SgrBI CCGCGG 1 cut(s) 490
SlaI CTCGAG 1 cut(s) 676
SmlI CTYRAG 2 cut(s) 167, 676
SmoI CTYRAG 2 cut(s) 167, 676
Sse9I AATT 4 cut(s) 51, 383, 600, 845
SsiI CCGC 8 cut(s) 188, 457, 487, 489, 505, 509, 625, 782
SspMI CTAG 1 cut(s) 389
StyD4I CCNGG 2 cut(s) 34, 282
StyI CCWWGG 1 cut(s) 97
TaaI ACNGT 2 cut(s) 108, 805
TaiI ACGT 2 cut(s) 276, 638
TaqI TCGA 6 cut(s) 29, 92, 144, 524, 644, 677
TasI AATT 4 cut(s) 51, 383, 600, 845
TatI WGTACW 1 cut(s) 680
TauI GCSGC 1 cut(s) 492
TfiI GAWTC 4 cut(s) 39, 82, 128, 706
TseI GCWGC 7 cut(s) 248, 462, 465, 482, 511, 613, 616
TspDTI ATGAA 4 cut(s) 45, 426, 593, 719
Van91I CCANNNNNTGG 1 cut(s) 70
XapI RAATTY 3 cut(s) 51, 383, 845
XbaI TCTAGA 1 cut(s) 388
XhoI CTCGAG 1 cut(s) 676
XspI CTAG 1 cut(s) 389
ZraI GACGTC 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.