Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0009322

Physical Location & Seq

Genomic Coordinates Forward (+)

3224513 .. 3229096

Gene Structure 4584 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1046300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 684 bp

ATGAGTACAGTCGTGCAGGGTTTCACCAAGTCGCTTGCCATGACTGTGCTCTCGGAGATCGGCGACAAGACCTTCTTCGCCGCTGCGATCCTTGCGATGCGCCATCCTAGAAGACTTGTTTTGGCAGGTTGCCTAGGAGCTCTGATTGTGATGACTATTCTTTCTGTTCTCGTCGGCTTGGCTGCTCCAAATCTGCTCTCAAGGAAATGGTCCCATCACATAACGACGGTGTTGTTCTTTGGTTTTGGACTATGGTCCTTATGGGATGCTTTTAAAGAAGATGGGGGTGATGATGAGCTTGCAGAAGTTGAAGCAGAGTTGGATGCTGATCTAAAGGCTAACACAGGAGGCAGCAAACGTAGTAAGGATGATGATGACAAGAAGCAGAGACGTTCATTTCTCTTGCAGTTCTTCTCACCAATCTTTTTGAAGGCCTTTTCCATTACTTTTTTCGGTGAATGGGGTGACAAGAGCCAGCTAGCTACCATCGGTCTGGCTGCAGATGAGAACCCATTAGGTGTGGTTCTTGGTGGAATCTTAGGACAAGCATTGTGTACGACTGCCGCTGTCCTTGGTGGAAAGAGCTTGGCATCTCAGATATCTGAGAAAATAGTTGGTTTATCAGGTGGAGTTCTTTTCATTGTTTTCGGAATCCAATCCTTCCTTTCGACGGTGGAGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0008150 GO:0009628 GO:0050896 GO:0080167

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UPF0016

Protein Analysis

228

Amino Acids

24.23

Weight (kDa)

5.63

Isoelectric Point (pI)

38.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GDT1	PF01169	10 - 83	1.2e-19	Divalent cation/proton antiporter GDT1
GDT1	PF01169	144 - 217	7.1e-24	Divalent cation/proton antiporter GDT1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009322)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G25520 AT1G68650
fragaria_vesca	FvH4_4g27780 FvH4_4g27780 FvH4_4g27780 FvH4_4g27780
malus_domestica	MD13G1045000.v1.1 MD16G1046300.v1.1
prunus_persica	Prupe.1G306900_v2.0.a1 Prupe.1G306900_v2.0.a1
pyrus_communis	pycom13g03980 pycom16g04090
rosa_chinensis	RchiOBHm_Chr4g0435641
rosa_laevigata	RLG00000006574
rosa_multiflora	Rmu_sc0000051.1_g000008
rosa_roxburghii	Rroxscaffold_5G00376860
rosa_rugosa	Rorug04G0287600
rosa_samantha	Rh4AG342700 Rh4BG351100 Rh4CG365700 Rh4DG345400
rosa_wichuraiana	Rw4G029910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	116
AciI	CCGC	2 cut(s)	81, 564
AclWI	GGATC	1 cut(s)	82
AfaI	GTAC	2 cut(s)	7, 556
AfiI	CCNNNNNNNGG	1 cut(s)	670
AgsI	TTSAA	2 cut(s)	311, 430
AluBI	AGCT	5 cut(s)	140, 298, 478, 482, 585
AluI	AGCT	5 cut(s)	140, 298, 478, 482, 585
Alw21I	GWGCWC	2 cut(s)	51, 142
Alw26I	GTCTC	1 cut(s)	382
AlwI	GGATC	1 cut(s)	82
AoxI	GGCC	1 cut(s)	432
ApeKI	GCWGC	4 cut(s)	83, 182, 351, 497
AspA2I	CCTAGG	1 cut(s)	133
AspLEI	GCGC	1 cut(s)	102
AspS9I	GGNCC	2 cut(s)	210, 255
AsuHPI	GGTGA	5 cut(s)	16, 299, 408, 467, 476
AsuNHI	GCTAGC	1 cut(s)	478
AvaII	GGWCC	2 cut(s)	210, 255
AvrII	CCTAGG	1 cut(s)	133
BanII	GRGCYC	1 cut(s)	142
BbsI	GAAGAC	1 cut(s)	118
Bbv12I	GWGCWC	2 cut(s)	51, 142
BbvI	GCAGC	4 cut(s)	70, 169, 363, 484
BccI	CCATC	4 cut(s)	111, 222, 275, 494
BcoDI	GTCTC	1 cut(s)	382
BfaI	CTAG	3 cut(s)	108, 134, 479
BfmI	CTRYAG	1 cut(s)	498
BfuAI	ACCTGC	1 cut(s)	116
BisI	GCNGC	6 cut(s)	81, 84, 183, 352, 498, 564
BlnI	CCTAGG	1 cut(s)	133
BlsI	GCNGC	6 cut(s)	82, 85, 184, 353, 499, 565
Bme18I	GGWCC	2 cut(s)	210, 255
BmgT120I	GGNCC	2 cut(s)	210, 255
BmiI	GGNNCC	1 cut(s)	212
BmsI	GCATC	4 cut(s)	87, 256, 313, 599
BmtI	GCTAGC	1 cut(s)	482
BoxI	GACNNNNGTC	1 cut(s)	253
BpiI	GAAGAC	1 cut(s)	118
BpuEI	CTTGAG	1 cut(s)	184
BsaBI	GATNNNNATC	1 cut(s)	327
BsaJI	CCNNGG	2 cut(s)	133, 571
Bsc4I	CCNNNNNNNGG	1 cut(s)	670
Bse8I	GATNNNNATC	1 cut(s)	327
BseDI	CCNNGG	2 cut(s)	133, 571
BseGI	GGATG	4 cut(s)	103, 271, 328, 373
BseJI	GATNNNNATC	1 cut(s)	327
BseLI	CCNNNNNNNGG	1 cut(s)	670
BseMII	CTCAG	2 cut(s)	594, 608
BseXI	GCAGC	4 cut(s)	70, 169, 363, 484
BsgI	GTGCAG	1 cut(s)	35
BshFI	GGCC	1 cut(s)	434
BsiHKAI	GWGCWC	2 cut(s)	51, 142
BslFI	GGGAC	1 cut(s)	196
BslI	CCNNNNNNNGG	1 cut(s)	670
BsmAI	GTCTC	1 cut(s)	382
BsmBI	CGTCTC	1 cut(s)	382
BsmFI	GGGAC	1 cut(s)	196
BsnI	GGCC	1 cut(s)	434
Bsp1286I	GDGCHC	2 cut(s)	51, 142
Bsp143I	GATC	3 cut(s)	57, 87, 328
BspACI	CCGC	2 cut(s)	81, 564
BspANI	GGCC	1 cut(s)	434
BspCNI	CTCAG	2 cut(s)	595, 607
BspLI	GGNNCC	1 cut(s)	212
BspMAI	CTGCAG	1 cut(s)	502
BspMI	ACCTGC	1 cut(s)	116
BspOI	GCTAGC	1 cut(s)	482
BspPI	GGATC	1 cut(s)	82
BssECI	CCNNGG	2 cut(s)	133, 571
BssMI	GATC	3 cut(s)	57, 87, 328
BssT1I	CCWWGG	2 cut(s)	133, 571
Bst4CI	ACNGT	4 cut(s)	10, 46, 229, 673
BstC8I	GCNNGC	4 cut(s)	36, 300, 476, 480
BstDEI	CTNAG	3 cut(s)	538, 594, 603
BstF5I	GGATG	4 cut(s)	103, 271, 328, 373
BstHHI	GCGC	1 cut(s)	102
BstKTI	GATC	3 cut(s)	60, 90, 331
BstMAI	GTCTC	1 cut(s)	382
BstMBI	GATC	3 cut(s)	57, 87, 328
BstMWI	GCNNNNNNNGC	1 cut(s)	92
BstPAI	GACNNNNGTC	1 cut(s)	253
BstSFI	CTRYAG	1 cut(s)	498
BstV1I	GCAGC	4 cut(s)	70, 169, 363, 484
BstV2I	GAAGAC	1 cut(s)	118
BstXI	CCANNNNNNTGG	1 cut(s)	493
BsuRI	GGCC	1 cut(s)	434
BtgZI	GCGATG	1 cut(s)	110
BtsCI	GGATG	4 cut(s)	103, 271, 328, 373
BveI	ACCTGC	1 cut(s)	116
Cac8I	GCNNGC	4 cut(s)	36, 300, 476, 480
CfoI	GCGC	1 cut(s)	102
Cfr13I	GGNCC	2 cut(s)	210, 255
Csp6I	GTAC	2 cut(s)	6, 555
CviAII	CATG	1 cut(s)	40
CviQI	GTAC	2 cut(s)	6, 555
DdeI	CTNAG	3 cut(s)	538, 594, 603
DpnI	GATC	3 cut(s)	59, 89, 330
DpnII	GATC	3 cut(s)	57, 87, 328
DraI	TTTAAA	1 cut(s)	274
Ecl136II	GAGCTC	1 cut(s)	140
Eco130I	CCWWGG	2 cut(s)	133, 571
Eco147I	AGGCCT	1 cut(s)	434
Eco24I	GRGCYC	1 cut(s)	142
Eco32I	GATATC	1 cut(s)	600
Eco47I	GGWCC	2 cut(s)	210, 255
Eco53kI	GAGCTC	1 cut(s)	140
EcoICRI	GAGCTC	1 cut(s)	140
EcoRV	GATATC	1 cut(s)	600
EcoT14I	CCWWGG	2 cut(s)	133, 571
EcoT38I	GRGCYC	1 cut(s)	142
ErhI	CCWWGG	2 cut(s)	133, 571
Esp3I	CGTCTC	1 cut(s)	382
FaeI	CATG	1 cut(s)	43
FaiI	YATR	4 cut(s)	41, 221, 253, 262
FalI	AAGNNNNNCTT	2 cut(s)	59, 91
FaqI	GGGAC	1 cut(s)	196
FatI	CATG	1 cut(s)	39
Fnu4HI	GCNGC	6 cut(s)	81, 84, 183, 352, 498, 564
FokI	GGATG	4 cut(s)	90, 278, 335, 380
FriOI	GRGCYC	1 cut(s)	142
Fsp4HI	GCNGC	6 cut(s)	81, 84, 183, 352, 498, 564
FspBI	CTAG	3 cut(s)	108, 134, 479
GlaI	GCGC	1 cut(s)	101
GluI	GCNGC	6 cut(s)	81, 84, 183, 352, 498, 564
HaeIII	GGCC	1 cut(s)	434
HhaI	GCGC	1 cut(s)	102
Hin1II	CATG	1 cut(s)	43
Hin6I	GCGC	1 cut(s)	100
HinP1I	GCGC	1 cut(s)	100
HinfI	GANTC	2 cut(s)	534, 651
HphI	GGTGA	5 cut(s)	16, 299, 408, 467, 476
Hpy166II	GTNNAC	1 cut(s)	555
Hpy188I	TCNGA	5 cut(s)	55, 144, 597, 604, 650
Hpy8I	GTNNAC	1 cut(s)	555
Hpy99I	CGWCG	3 cut(s)	176, 229, 673
HpyAV	CCTTC	3 cut(s)	82, 424, 670
HpyCH4III	ACNGT	4 cut(s)	10, 46, 229, 673
HpyCH4IV	ACGT	2 cut(s)	358, 391
HpyCH4V	TGCA	4 cut(s)	16, 302, 406, 500
HpyF10VI	GCNNNNNNNGC	1 cut(s)	92
HpyF3I	CTNAG	3 cut(s)	538, 594, 603
HpySE526I	ACGT	2 cut(s)	358, 391
Hsp92II	CATG	1 cut(s)	43
HspAI	GCGC	1 cut(s)	100
Kzo9I	GATC	3 cut(s)	57, 87, 328
LmnI	GCTCC	2 cut(s)	137, 190
LpnPI	CCDG	6 cut(s)	2, 111, 330, 479, 488, 609
Lsp1109I	GCAGC	4 cut(s)	70, 169, 363, 484
LweI	GCATC	4 cut(s)	87, 256, 313, 599
MaeI	CTAG	3 cut(s)	108, 134, 479
MaeII	ACGT	2 cut(s)	358, 391
MaeIII	GTNAC	1 cut(s)	464
MalI	GATC	3 cut(s)	59, 89, 330
MboI	GATC	3 cut(s)	57, 87, 328
MboII	GAAGA	4 cut(s)	67, 123, 290, 403
MhlI	GDGCHC	2 cut(s)	51, 142
MmeI	TCCRAC	1 cut(s)	300
MnlI	CCTC	2 cut(s)	341, 670
MseI	TTAA	1 cut(s)	273
MslI	CAYNNNNRTG	1 cut(s)	44
MspA1I	CMGCKG	2 cut(s)	83, 566
MwoI	GCNNNNNNNGC	1 cut(s)	92
NdeII	GATC	3 cut(s)	57, 87, 328
NheI	GCTAGC	1 cut(s)	478
NlaIII	CATG	1 cut(s)	43
NlaIV	GGNNCC	1 cut(s)	212
NmuCI	GTSAC	1 cut(s)	464
PceI	AGGCCT	1 cut(s)	434
PfeI	GAWTC	2 cut(s)	534, 651
PkrI	GCNGC	6 cut(s)	82, 85, 184, 353, 499, 565
PshAI	GACNNNNGTC	1 cut(s)	253
Psp124BI	GAGCTC	1 cut(s)	142
PspN4I	GGNNCC	1 cut(s)	212
PspPI	GGNCC	2 cut(s)	210, 255
PstI	CTGCAG	1 cut(s)	502
RsaI	GTAC	2 cut(s)	7, 556
RsaNI	GTAC	2 cut(s)	6, 555
RseI	CAYNNNNRTG	1 cut(s)	44
SacI	GAGCTC	1 cut(s)	142
SaqAI	TTAA	1 cut(s)	273
SatI	GCNGC	6 cut(s)	81, 84, 183, 352, 498, 564
Sau3AI	GATC	3 cut(s)	57, 87, 328
Sau96I	GGNCC	2 cut(s)	210, 255
SduI	GDGCHC	2 cut(s)	51, 142
SfaNI	GCATC	4 cut(s)	87, 256, 313, 599
SfcI	CTRYAG	1 cut(s)	498
SinI	GGWCC	2 cut(s)	210, 255
SmiMI	CAYNNNNRTG	1 cut(s)	44
SmlI	CTYRAG	1 cut(s)	199
SmoI	CTYRAG	1 cut(s)	199
SseBI	AGGCCT	1 cut(s)	434
SsiI	CCGC	2 cut(s)	81, 564
SspMI	CTAG	3 cut(s)	108, 134, 479
SstI	GAGCTC	1 cut(s)	142
StuI	AGGCCT	1 cut(s)	434
StyI	CCWWGG	2 cut(s)	133, 571
TaaI	ACNGT	4 cut(s)	10, 46, 229, 673
TaiI	ACGT	2 cut(s)	361, 394
TaqI	TCGA	1 cut(s)	668
TaqII	GACCGA	1 cut(s)	479
TatI	WGTACW	1 cut(s)	5
TauI	GCSGC	2 cut(s)	83, 566
TfiI	GAWTC	2 cut(s)	534, 651
Tru1I	TTAA	1 cut(s)	273
Tru9I	TTAA	1 cut(s)	273
TseFI	GTSAC	1 cut(s)	464
TseI	GCWGC	4 cut(s)	83, 182, 351, 497
Tsp45I	GTSAC	1 cut(s)	464
TspDTI	ATGAA	2 cut(s)	384, 628
VpaK11BI	GGWCC	2 cut(s)	210, 255
XmaJI	CCTAGG	1 cut(s)	133
XspI	CTAG	3 cut(s)	108, 134, 479

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.