pycom13g03980

GDT1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0009322
Physical Location & Seq
Forward (+)
2624950 .. 2627183
2234 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g03980.1

Sequence Viewer

Length: 684 bp
ATGAGTGCAGTCGTGCAGGGTTTCACCAAGTCACTTGCCATGACTGTGCTCTCGGAGATCGGCGACAAGACCTTCTTCGCCGCCGCGATCCTTGCGATGCGCCATCCTAGAAGACTTGTTTTGGCCGGTTGCCTAGGAGCTCTGATTGTGATGACTATTCTTTCTGTTCTCGTTGGCTTGGCTGCTCCAAATCTGCTCTCCCGGAAATGGTCCCATCACATAACAACAGTTTTGTTCTTTGGCTTTGGACTATGGTCCTTATGGGACGCTTTTAAAGAAGATGGGGGTGACGATGAGCTTGCAGAAGTTGAAGCAGAATTGGATGCTGATCTAAAGGCTAACGCAGGAGGCAGCAAACGTACTAAGGATGATGATGACAAGAAGCAGAGACGTTCATTTCTCTTGCAGTTCTTCTCACCGATCTTTTTGAAGGCCTTTTCCATTACATTTTTCGGTGAATGGGGCGACAAGAGCCAGCTAGCCACCATCGGTCTGGCGGCAGATGAGAACCCATTAGGCGTGGTTCTTGGTGGAATCTTAGGACAGGCATTATGTACGACTGCCGCTGTCCTTGGTGGAAAGAGCTTGGCATCTCAGATATCGGAGAAAATAGTTGGTTTATCGGGTGGAGTTCTTTTCATTGTTTTCGGAATCCAATCCTTCCTTTCGACAGTGGAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009628 GO:0050896 GO:0080167
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0016

Protein Analysis

228

Amino Acids

24.19

Weight (kDa)

5.63

Isoelectric Point (pI)

36.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDT1 PF01169 10 - 83 1.2e-19 Divalent cation/proton antiporter GDT1
GDT1 PF01169 144 - 217 7.1e-24 Divalent cation/proton antiporter GDT1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 86
AciI CCGC 4 cut(s) 81, 84, 497, 564
AclWI GGATC 1 cut(s) 82
AcoI YGGCCR 1 cut(s) 123
AfaI GTAC 2 cut(s) 361, 556
AfiI CCNNNNNNNGG 1 cut(s) 207
AgsI TTSAA 2 cut(s) 311, 430
AluBI AGCT 4 cut(s) 140, 298, 478, 585
AluI AGCT 4 cut(s) 140, 298, 478, 585
Alw21I GWGCWC 2 cut(s) 51, 142
Alw26I GTCTC 1 cut(s) 382
AlwI GGATC 1 cut(s) 82
AoxI GGCC 2 cut(s) 123, 432
ApeKI GCWGC 2 cut(s) 182, 351
AspA2I CCTAGG 1 cut(s) 133
AspLEI GCGC 1 cut(s) 102
AspS9I GGNCC 2 cut(s) 210, 255
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 4 cut(s) 16, 299, 408, 467
AsuNHI GCTAGC 1 cut(s) 478
AvaII GGWCC 2 cut(s) 210, 255
AvrII CCTAGG 1 cut(s) 133
BanII GRGCYC 1 cut(s) 142
BbsI GAAGAC 1 cut(s) 118
Bbv12I GWGCWC 2 cut(s) 51, 142
BbvI GCAGC 2 cut(s) 169, 363
BccI CCATC 4 cut(s) 111, 222, 275, 494
BcgI CGANNNNNNTGC 2 cut(s) 281, 315
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 1 cut(s) 382
BfaI CTAG 3 cut(s) 108, 134, 479
BisI GCNGC 6 cut(s) 81, 84, 183, 352, 498, 564
BlnI CCTAGG 1 cut(s) 133
BlsI GCNGC 6 cut(s) 82, 85, 184, 353, 499, 565
Bme1390I CCNGG 1 cut(s) 202
Bme18I GGWCC 2 cut(s) 210, 255
BmgT120I GGNCC 2 cut(s) 210, 255
BmiI GGNNCC 1 cut(s) 212
BmrFI CCNGG 1 cut(s) 202
BmsI GCATC 3 cut(s) 87, 313, 599
BmtI GCTAGC 1 cut(s) 482
BoxI GACNNNNGTC 1 cut(s) 253
BpiI GAAGAC 1 cut(s) 118
BpuMI CCSGG 1 cut(s) 202
BsaBI GATNNNNATC 1 cut(s) 327
BsaJI CCNNGG 2 cut(s) 133, 571
Bsc4I CCNNNNNNNGG 1 cut(s) 207
Bse118I RCCGGY 1 cut(s) 125
Bse8I GATNNNNATC 1 cut(s) 327
BseDI CCNNGG 2 cut(s) 133, 571
BseGI GGATG 3 cut(s) 103, 328, 373
BseJI GATNNNNATC 1 cut(s) 327
BseLI CCNNNNNNNGG 1 cut(s) 207
BseMII CTCAG 1 cut(s) 608
BseXI GCAGC 2 cut(s) 169, 363
BsgI GTGCAG 2 cut(s) 27, 35
Bsh1236I CGCG 1 cut(s) 86
BshFI GGCC 2 cut(s) 125, 434
BsiHKAI GWGCWC 2 cut(s) 51, 142
BsiSI CCGG 2 cut(s) 126, 202
BslFI GGGAC 2 cut(s) 196, 278
BslI CCNNNNNNNGG 1 cut(s) 207
BsmAI GTCTC 1 cut(s) 382
BsmBI CGTCTC 1 cut(s) 382
BsmFI GGGAC 2 cut(s) 196, 278
BsnI GGCC 2 cut(s) 125, 434
Bsp1286I GDGCHC 2 cut(s) 51, 142
Bsp143I GATC 4 cut(s) 57, 87, 328, 420
BspACI CCGC 4 cut(s) 81, 84, 497, 564
BspANI GGCC 2 cut(s) 125, 434
BspCNI CTCAG 1 cut(s) 607
BspFNI CGCG 1 cut(s) 86
BspLI GGNNCC 1 cut(s) 212
BspOI GCTAGC 1 cut(s) 482
BspPI GGATC 1 cut(s) 82
BsrFI RCCGGY 1 cut(s) 125
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 2 cut(s) 133, 571
BssMI GATC 4 cut(s) 57, 87, 328, 420
BssT1I CCWWGG 2 cut(s) 133, 571
Bst4CI ACNGT 3 cut(s) 46, 229, 673
BstC8I GCNNGC 3 cut(s) 300, 476, 480
BstDEI CTNAG 3 cut(s) 363, 538, 594
BstF5I GGATG 3 cut(s) 103, 328, 373
BstFNI CGCG 1 cut(s) 86
BstHHI GCGC 1 cut(s) 102
BstKTI GATC 4 cut(s) 60, 90, 331, 423
BstMAI GTCTC 1 cut(s) 382
BstMBI GATC 4 cut(s) 57, 87, 328, 420
BstMWI GCNNNNNNNGC 2 cut(s) 92, 471
BstPAI GACNNNNGTC 1 cut(s) 253
BstSCI CCNGG 1 cut(s) 200
BstUI CGCG 1 cut(s) 86
BstV1I GCAGC 2 cut(s) 169, 363
BstV2I GAAGAC 1 cut(s) 118
BstXI CCANNNNNNTGG 1 cut(s) 493
BsuRI GGCC 2 cut(s) 125, 434
BtgZI GCGATG 1 cut(s) 110
BtsCI GGATG 3 cut(s) 103, 328, 373
BtsIMutI CAGTG 1 cut(s) 678
Cac8I GCNNGC 3 cut(s) 300, 476, 480
CfoI GCGC 1 cut(s) 102
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 2 cut(s) 210, 255
CseI GACGC 1 cut(s) 275
Csp6I GTAC 2 cut(s) 360, 555
CviAII CATG 1 cut(s) 40
CviQI GTAC 2 cut(s) 360, 555
DdeI CTNAG 3 cut(s) 363, 538, 594
DpnI GATC 4 cut(s) 59, 89, 330, 422
DpnII GATC 4 cut(s) 57, 87, 328, 420
DraI TTTAAA 1 cut(s) 274
EaeI YGGCCR 1 cut(s) 123
Ecl136II GAGCTC 1 cut(s) 140
Eco130I CCWWGG 2 cut(s) 133, 571
Eco147I AGGCCT 1 cut(s) 434
Eco24I GRGCYC 1 cut(s) 142
Eco32I GATATC 1 cut(s) 600
Eco47I GGWCC 2 cut(s) 210, 255
Eco53kI GAGCTC 1 cut(s) 140
EcoICRI GAGCTC 1 cut(s) 140
EcoRV GATATC 1 cut(s) 600
EcoT14I CCWWGG 2 cut(s) 133, 571
EcoT38I GRGCYC 1 cut(s) 142
ErhI CCWWGG 2 cut(s) 133, 571
Esp3I CGTCTC 1 cut(s) 382
FaeI CATG 1 cut(s) 43
FaiI YATR 5 cut(s) 41, 221, 253, 262, 553
FalI AAGNNNNNCTT 2 cut(s) 59, 91
FaqI GGGAC 2 cut(s) 196, 278
FatI CATG 1 cut(s) 39
Fnu4HI GCNGC 6 cut(s) 81, 84, 183, 352, 498, 564
FokI GGATG 3 cut(s) 90, 335, 380
FriOI GRGCYC 1 cut(s) 142
Fsp4HI GCNGC 6 cut(s) 81, 84, 183, 352, 498, 564
FspBI CTAG 3 cut(s) 108, 134, 479
GlaI GCGC 1 cut(s) 101
GluI GCNGC 6 cut(s) 81, 84, 183, 352, 498, 564
HaeIII GGCC 2 cut(s) 125, 434
HapII CCGG 2 cut(s) 126, 202
HgaI GACGC 1 cut(s) 275
HhaI GCGC 1 cut(s) 102
Hin1II CATG 1 cut(s) 43
Hin6I GCGC 1 cut(s) 100
HinP1I GCGC 1 cut(s) 100
HinfI GANTC 2 cut(s) 534, 651
HpaII CCGG 2 cut(s) 126, 202
HphI GGTGA 4 cut(s) 16, 299, 408, 467
Hpy188I TCNGA 5 cut(s) 55, 144, 597, 604, 650
HpyAV CCTTC 3 cut(s) 82, 424, 670
HpyCH4III ACNGT 3 cut(s) 46, 229, 673
HpyCH4IV ACGT 2 cut(s) 358, 391
HpyCH4V TGCA 4 cut(s) 8, 16, 302, 406
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 471
HpyF3I CTNAG 3 cut(s) 363, 538, 594
HpySE526I ACGT 2 cut(s) 358, 391
Hsp92II CATG 1 cut(s) 43
HspAI GCGC 1 cut(s) 100
Kzo9I GATC 4 cut(s) 57, 87, 328, 420
LmnI GCTCC 2 cut(s) 137, 190
LpnPI CCDG 7 cut(s) 2, 139, 215, 330, 479, 488, 530
Lsp1109I GCAGC 2 cut(s) 169, 363
LweI GCATC 3 cut(s) 87, 313, 599
MaeI CTAG 3 cut(s) 108, 134, 479
MaeII ACGT 2 cut(s) 358, 391
MaeIII GTNAC 2 cut(s) 30, 287
MalI GATC 4 cut(s) 59, 89, 330, 422
MboI GATC 4 cut(s) 57, 87, 328, 420
MboII GAAGA 4 cut(s) 67, 123, 290, 403
MhlI GDGCHC 2 cut(s) 51, 142
MluCI AATT 1 cut(s) 317
MnlI CCTC 2 cut(s) 341, 670
MseI TTAA 1 cut(s) 273
MslI CAYNNNNRTG 1 cut(s) 44
MspA1I CMGCKG 1 cut(s) 566
MspI CCGG 2 cut(s) 126, 202
MspR9I CCNGG 1 cut(s) 202
MvnI CGCG 1 cut(s) 86
MwoI GCNNNNNNNGC 2 cut(s) 92, 471
NciI CCSGG 1 cut(s) 202
NdeII GATC 4 cut(s) 57, 87, 328, 420
NheI GCTAGC 1 cut(s) 478
NlaIII CATG 1 cut(s) 43
NlaIV GGNNCC 1 cut(s) 212
NmuCI GTSAC 2 cut(s) 30, 287
PceI AGGCCT 1 cut(s) 434
PfeI GAWTC 2 cut(s) 534, 651
PfoI TCCNGGA 1 cut(s) 200
PkrI GCNGC 6 cut(s) 82, 85, 184, 353, 499, 565
PshAI GACNNNNGTC 1 cut(s) 253
Psp124BI GAGCTC 1 cut(s) 142
PspN4I GGNNCC 1 cut(s) 212
PspPI GGNCC 2 cut(s) 210, 255
RsaI GTAC 2 cut(s) 361, 556
RsaNI GTAC 2 cut(s) 360, 555
RseI CAYNNNNRTG 1 cut(s) 44
SacI GAGCTC 1 cut(s) 142
SaqAI TTAA 1 cut(s) 273
SatI GCNGC 6 cut(s) 81, 84, 183, 352, 498, 564
Sau3AI GATC 4 cut(s) 57, 87, 328, 420
Sau96I GGNCC 2 cut(s) 210, 255
ScrFI CCNGG 1 cut(s) 202
SduI GDGCHC 2 cut(s) 51, 142
SetI ASST 7 cut(s) 74, 142, 300, 361, 394, 480, 587
SfaNI GCATC 3 cut(s) 87, 313, 599
SinI GGWCC 2 cut(s) 210, 255
SmiMI CAYNNNNRTG 1 cut(s) 44
Sse9I AATT 1 cut(s) 317
SseBI AGGCCT 1 cut(s) 434
SsiI CCGC 4 cut(s) 81, 84, 497, 564
SspMI CTAG 3 cut(s) 108, 134, 479
SstI GAGCTC 1 cut(s) 142
StuI AGGCCT 1 cut(s) 434
StyD4I CCNGG 1 cut(s) 200
StyI CCWWGG 2 cut(s) 133, 571
TaaI ACNGT 3 cut(s) 46, 229, 673
TaiI ACGT 2 cut(s) 361, 394
TaqI TCGA 1 cut(s) 668
TaqII GACCGA 1 cut(s) 479
TasI AATT 1 cut(s) 317
TauI GCSGC 4 cut(s) 83, 86, 500, 566
TfiI GAWTC 2 cut(s) 534, 651
Tru1I TTAA 1 cut(s) 273
Tru9I TTAA 1 cut(s) 273
TscAI CASTG 1 cut(s) 678
TseFI GTSAC 2 cut(s) 30, 287
TseI GCWGC 2 cut(s) 182, 351
Tsp45I GTSAC 2 cut(s) 30, 287
TspDTI ATGAA 2 cut(s) 384, 628
TspRI CASTG 1 cut(s) 678
VpaK11BI GGWCC 2 cut(s) 210, 255
XcmI CCANNNNNNNNNTGG 1 cut(s) 490
XmaJI CCTAGG 1 cut(s) 133
XspI CTAG 3 cut(s) 108, 134, 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.