Rh4BG351100

GDT1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0009322
Physical Location & Seq
Forward (+)
51215171 .. 51218276
3106 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG351100.1

Sequence Viewer

Length: 495 bp
ATGAGCACCGTCGTCCAGGGTTTCACCAAGTCACTTGCGATGACTGTGCTATCGGAGATCGGCGACAAGACCTTCTTCGCCGCCGCGATCCTTGCGATGCGCCATCCCAGGAGACTCGTTTTGTCTGGTTGCCTAGGAGCTCTAATTGTGATGACTATTCTTTCTGTTCTTGTTGGCTGGGCTGCTCCAAATTTGCTCTCGCGGACATGGACCCATCACATAACAACAGTGTTGTTCTTTGGGTTCGGCCTATGGTCCTTATGGGATGCTTTCAAAGGAGACGGGGGTGATGATGAACTTGCAGAAGTTGAAGCGGAATTGGATGCCAAGGCGGGATCCACCAAACAGAGTAGTAAGGATGACGATGAAACGAAAAAGCAGAATCGTTCATTTATCTTGCAATTCTTCTCACCCATTTTTTTAAAGGCCTTTTCTATTACTTTTTTTGGTGAATGGGGTGACAAGAGCCAGGTGAGCATGTGCCCAACTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009628 GO:0050896 GO:0080167
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0016

Protein Analysis

164

Amino Acids

18.06

Weight (kDa)

5.58

Isoelectric Point (pI)

40.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDT1 PF01169 10 - 83 8.2e-20 Divalent cation/proton antiporter GDT1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 86, 202
AciI CCGC 5 cut(s) 81, 84, 202, 314, 332
AclWI GGATC 3 cut(s) 82, 330, 343
AcsI RAATTY 1 cut(s) 190
AgsI TTSAA 2 cut(s) 274, 311
AjnI CCWGG 3 cut(s) 15, 107, 468
AluBI AGCT 1 cut(s) 140
AluI AGCT 1 cut(s) 140
Alw21I GWGCWC 2 cut(s) 8, 142
Alw26I GTCTC 2 cut(s) 106, 273
AlwI GGATC 3 cut(s) 82, 330, 343
AoxI GGCC 2 cut(s) 247, 426
ApeKI GCWGC 1 cut(s) 182
ApoI RAATTY 1 cut(s) 190
AspA2I CCTAGG 1 cut(s) 133
AspLEI GCGC 1 cut(s) 102
AspS9I GGNCC 2 cut(s) 210, 255
AsuHPI GGTGA 6 cut(s) 16, 299, 402, 461, 470, 484
AvaII GGWCC 2 cut(s) 210, 255
AvrII CCTAGG 1 cut(s) 133
BaeGI GKGCMC 1 cut(s) 485
BamHI GGATCC 1 cut(s) 335
BanII GRGCYC 1 cut(s) 142
Bbv12I GWGCWC 2 cut(s) 8, 142
BbvI GCAGC 1 cut(s) 169
BccI CCATC 2 cut(s) 111, 222
BcgI CGANNNNNNTGC 2 cut(s) 28, 62
BciT130I CCWGG 3 cut(s) 17, 109, 470
BcoDI GTCTC 2 cut(s) 106, 273
BfaI CTAG 2 cut(s) 134, 493
BisI GCNGC 3 cut(s) 81, 84, 183
BlnI CCTAGG 1 cut(s) 133
BlsI GCNGC 3 cut(s) 82, 85, 184
Bme1390I CCNGG 3 cut(s) 17, 109, 470
Bme18I GGWCC 2 cut(s) 210, 255
BmgT120I GGNCC 2 cut(s) 210, 255
BmiI GGNNCC 2 cut(s) 212, 337
BmrFI CCNGG 3 cut(s) 17, 109, 470
BmsI GCATC 3 cut(s) 87, 256, 313
BsaJI CCNNGG 4 cut(s) 16, 107, 133, 327
BseBI CCWGG 3 cut(s) 17, 109, 470
BseDI CCNNGG 4 cut(s) 16, 107, 133, 327
BseGI GGATG 4 cut(s) 103, 271, 328, 364
BseSI GKGCMC 1 cut(s) 485
BseXI GCAGC 1 cut(s) 169
BseYI CCCAGC 1 cut(s) 177
Bsh1236I CGCG 2 cut(s) 86, 202
BshFI GGCC 2 cut(s) 249, 428
BsiHKAI GWGCWC 2 cut(s) 8, 142
BsmAI GTCTC 2 cut(s) 106, 273
BsmBI CGTCTC 1 cut(s) 273
BsnI GGCC 2 cut(s) 249, 428
Bsp1286I GDGCHC 3 cut(s) 8, 142, 485
Bsp143I GATC 3 cut(s) 57, 87, 335
BspACI CCGC 5 cut(s) 81, 84, 202, 314, 332
BspANI GGCC 2 cut(s) 249, 428
BspFNI CGCG 2 cut(s) 86, 202
BspLI GGNNCC 2 cut(s) 212, 337
BspPI GGATC 3 cut(s) 82, 330, 343
BssECI CCNNGG 4 cut(s) 16, 107, 133, 327
BssMI GATC 3 cut(s) 57, 87, 335
BssT1I CCWWGG 2 cut(s) 133, 327
Bst2UI CCWGG 3 cut(s) 17, 109, 470
Bst4CI ACNGT 3 cut(s) 10, 46, 229
BstF5I GGATG 4 cut(s) 103, 271, 328, 364
BstFNI CGCG 2 cut(s) 86, 202
BstHHI GCGC 1 cut(s) 102
BstKTI GATC 3 cut(s) 60, 90, 338
BstMAI GTCTC 2 cut(s) 106, 273
BstMBI GATC 3 cut(s) 57, 87, 335
BstMWI GCNNNNNNNGC 2 cut(s) 92, 474
BstNI CCWGG 3 cut(s) 17, 109, 470
BstNSI RCATGY 1 cut(s) 481
BstSCI CCNGG 3 cut(s) 15, 107, 468
BstSLI GKGCMC 1 cut(s) 485
BstUI CGCG 2 cut(s) 86, 202
BstV1I GCAGC 1 cut(s) 169
BstX2I RGATCY 1 cut(s) 335
BstYI RGATCY 1 cut(s) 335
BsuRI GGCC 2 cut(s) 249, 428
BtgZI GCGATG 2 cut(s) 53, 110
BtsCI GGATG 4 cut(s) 103, 271, 328, 364
BtsIMutI CAGTG 1 cut(s) 234
CfoI GCGC 1 cut(s) 102
Cfr13I GGNCC 2 cut(s) 210, 255
CviAII CATG 2 cut(s) 207, 478
CviJI RGCY 6 cut(s) 140, 177, 182, 249, 428, 468
CviKI_1 RGCY 6 cut(s) 140, 177, 182, 249, 428, 468
DpnI GATC 3 cut(s) 59, 89, 337
DpnII GATC 3 cut(s) 57, 87, 335
DraI TTTAAA 1 cut(s) 423
Ecl136II GAGCTC 1 cut(s) 140
Eco130I CCWWGG 2 cut(s) 133, 327
Eco147I AGGCCT 1 cut(s) 428
Eco24I GRGCYC 1 cut(s) 142
Eco47I GGWCC 2 cut(s) 210, 255
Eco53kI GAGCTC 1 cut(s) 140
EcoICRI GAGCTC 1 cut(s) 140
EcoRII CCWGG 3 cut(s) 15, 107, 468
EcoT14I CCWWGG 2 cut(s) 133, 327
EcoT38I GRGCYC 1 cut(s) 142
ErhI CCWWGG 2 cut(s) 133, 327
Esp3I CGTCTC 1 cut(s) 273
FaeI CATG 2 cut(s) 210, 481
FaiI YATR 5 cut(s) 208, 221, 253, 262, 479
FalI AAGNNNNNCTT 2 cut(s) 59, 91
FatI CATG 2 cut(s) 206, 477
FauI CCCGC 1 cut(s) 325
Fnu4HI GCNGC 3 cut(s) 81, 84, 183
FokI GGATG 4 cut(s) 90, 278, 335, 371
FriOI GRGCYC 1 cut(s) 142
Fsp4HI GCNGC 3 cut(s) 81, 84, 183
FspBI CTAG 2 cut(s) 134, 493
GlaI GCGC 1 cut(s) 101
GluI GCNGC 3 cut(s) 81, 84, 183
GsaI CCCAGC 1 cut(s) 181
HaeIII GGCC 2 cut(s) 249, 428
HhaI GCGC 1 cut(s) 102
Hin1II CATG 2 cut(s) 210, 481
Hin6I GCGC 1 cut(s) 100
HinP1I GCGC 1 cut(s) 100
HinfI GANTC 2 cut(s) 114, 382
HphI GGTGA 6 cut(s) 16, 299, 402, 461, 470, 484
Hpy188I TCNGA 1 cut(s) 55
Hpy99I CGWCG 1 cut(s) 14
HpyAV CCTTC 1 cut(s) 82
HpyCH4III ACNGT 3 cut(s) 10, 46, 229
HpyCH4V TGCA 2 cut(s) 302, 400
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 474
Hsp92II CATG 2 cut(s) 210, 481
HspAI GCGC 1 cut(s) 100
Kzo9I GATC 3 cut(s) 57, 87, 335
LmnI GCTCC 2 cut(s) 137, 190
LpnPI CCDG 8 cut(s) 2, 29, 94, 111, 121, 163, 455, 482
Lsp1109I GCAGC 1 cut(s) 169
LweI GCATC 3 cut(s) 87, 256, 313
MaeI CTAG 2 cut(s) 134, 493
MaeIII GTNAC 2 cut(s) 30, 458
MalI GATC 3 cut(s) 59, 89, 337
MboI GATC 3 cut(s) 57, 87, 335
MboII GAAGA 2 cut(s) 67, 397
MflI RGATCY 1 cut(s) 335
MhlI GDGCHC 3 cut(s) 8, 142, 485
MluCI AATT 4 cut(s) 144, 190, 317, 401
MlyI GAGTC 1 cut(s) 108
MseI TTAA 1 cut(s) 422
MspR9I CCNGG 3 cut(s) 17, 109, 470
MvaI CCWGG 3 cut(s) 17, 109, 470
MvnI CGCG 2 cut(s) 86, 202
MwoI GCNNNNNNNGC 2 cut(s) 92, 474
NdeII GATC 3 cut(s) 57, 87, 335
NlaIII CATG 2 cut(s) 210, 481
NlaIV GGNNCC 2 cut(s) 212, 337
NmuCI GTSAC 2 cut(s) 30, 458
NspI RCATGY 1 cut(s) 481
PceI AGGCCT 1 cut(s) 428
PfeI GAWTC 1 cut(s) 382
PkrI GCNGC 3 cut(s) 82, 85, 184
PleI GAGTC 1 cut(s) 108
PpsI GAGTC 1 cut(s) 108
Psp124BI GAGCTC 1 cut(s) 142
Psp6I CCWGG 3 cut(s) 15, 107, 468
PspFI CCCAGC 1 cut(s) 177
PspGI CCWGG 3 cut(s) 15, 107, 468
PspN4I GGNNCC 2 cut(s) 212, 337
PspPI GGNCC 2 cut(s) 210, 255
PsuI RGATCY 1 cut(s) 335
SacI GAGCTC 1 cut(s) 142
SaqAI TTAA 1 cut(s) 422
SatI GCNGC 3 cut(s) 81, 84, 183
Sau3AI GATC 3 cut(s) 57, 87, 335
Sau96I GGNCC 2 cut(s) 210, 255
SchI GAGTC 1 cut(s) 108
ScrFI CCNGG 3 cut(s) 17, 109, 470
SduI GDGCHC 3 cut(s) 8, 142, 485
SetI ASST 3 cut(s) 74, 142, 474
SfaNI GCATC 3 cut(s) 87, 256, 313
SinI GGWCC 2 cut(s) 210, 255
Sse9I AATT 4 cut(s) 144, 190, 317, 401
SseBI AGGCCT 1 cut(s) 428
SsiI CCGC 5 cut(s) 81, 84, 202, 314, 332
SspMI CTAG 2 cut(s) 134, 493
SstI GAGCTC 1 cut(s) 142
StuI AGGCCT 1 cut(s) 428
StyD4I CCNGG 3 cut(s) 15, 107, 468
StyI CCWWGG 2 cut(s) 133, 327
TaaI ACNGT 3 cut(s) 10, 46, 229
TasI AATT 4 cut(s) 144, 190, 317, 401
TauI GCSGC 2 cut(s) 83, 86
TfiI GAWTC 1 cut(s) 382
Tru1I TTAA 1 cut(s) 422
Tru9I TTAA 1 cut(s) 422
TscAI CASTG 1 cut(s) 234
TseFI GTSAC 2 cut(s) 30, 458
TseI GCWGC 1 cut(s) 182
Tsp45I GTSAC 2 cut(s) 30, 458
TspDTI ATGAA 3 cut(s) 309, 378, 381
TspRI CASTG 1 cut(s) 234
VpaK11BI GGWCC 2 cut(s) 210, 255
XapI RAATTY 1 cut(s) 190
XceI RCATGY 1 cut(s) 481
XmaJI CCTAGG 1 cut(s) 133
XspI CTAG 2 cut(s) 134, 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.