Rh4DG345400

GDT1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0009322
Physical Location & Seq
Forward (+)
56703775 .. 56706915
3141 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG345400.1

Sequence Viewer

Length: 573 bp
ATGCGCCATCCCAGGAGACTTGTTTTGTCTGGTTGCCTAGGAGCTCTAATTGTGATGACTATTCTTTCTGTTCTTGTTGGCTGGGCTGCTCCAAATTTGCTCTCGCGGACATGGACCCATCACATAACAACAGTGTTGTTCTTTGGGTTCGGCCTATGGTCCTTATGGGATGCTTTCAAAGGAGACGGGGGTGATGATGAACTTGCAGAAGTTGAAGCGGAATTGGATGCCAAGGCGGGATCCACCAAACAGAGTAGTAAGGATGACGATGAATCCAAAAAGCAGAATCGTTCATTTATCTTGCAATTCTTCTCACCCATTTTTTTAAAGGCCTTTTCTATTACTTTTTTCGGTGAATGGGGTGACAAGAGCCAGCTAGCTACTATTGGTTTGGCTGCAGATGAAAACCCATTAGGTGTGGTTATTGGTGGAATCATAGGACAAGCGTTGTGTACCACTGCTGCTGTCCTTGGTGGAAAGAGCTTGGCATCTCAGATATCTGAGAAAATTGTTGCATTATCAGGTGGAGTTCTTTTCATTGTTTTCGGAATCCAATCCTTCCTTTCGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009628 GO:0050896 GO:0080167
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0016

Protein Analysis

190

Amino Acids

20.3

Weight (kDa)

5.57

Isoelectric Point (pI)

35.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDT1 PF01169 1 - 51 3.3e-07 Divalent cation/proton antiporter GDT1
GDT1 PF01169 110 - 183 4.7e-24 Divalent cation/proton antiporter GDT1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 106
AciI CCGC 3 cut(s) 106, 218, 236
AclWI GGATC 2 cut(s) 234, 247
AcsI RAATTY 1 cut(s) 94
AfaI GTAC 1 cut(s) 454
AgsI TTSAA 2 cut(s) 178, 215
AjnI CCWGG 1 cut(s) 11
AluBI AGCT 4 cut(s) 44, 376, 380, 483
AluI AGCT 4 cut(s) 44, 376, 380, 483
Alw21I GWGCWC 1 cut(s) 46
Alw26I GTCTC 2 cut(s) 10, 177
AlwI GGATC 2 cut(s) 234, 247
AoxI GGCC 2 cut(s) 151, 330
ApeKI GCWGC 3 cut(s) 86, 395, 461
ApoI RAATTY 1 cut(s) 94
AspA2I CCTAGG 1 cut(s) 37
AspLEI GCGC 1 cut(s) 6
AspS9I GGNCC 2 cut(s) 114, 159
AsuHPI GGTGA 4 cut(s) 203, 306, 365, 374
AsuNHI GCTAGC 1 cut(s) 376
AvaII GGWCC 2 cut(s) 114, 159
AvrII CCTAGG 1 cut(s) 37
BamHI GGATCC 1 cut(s) 239
BanII GRGCYC 1 cut(s) 46
Bbv12I GWGCWC 1 cut(s) 46
BbvI GCAGC 3 cut(s) 73, 382, 448
BccI CCATC 2 cut(s) 15, 126
BciT130I CCWGG 1 cut(s) 13
BcoDI GTCTC 2 cut(s) 10, 177
BfaI CTAG 2 cut(s) 38, 377
BfmI CTRYAG 1 cut(s) 396
BisI GCNGC 3 cut(s) 87, 396, 462
BlnI CCTAGG 1 cut(s) 37
BlsI GCNGC 3 cut(s) 88, 397, 463
Bme1390I CCNGG 1 cut(s) 13
Bme18I GGWCC 2 cut(s) 114, 159
BmgT120I GGNCC 2 cut(s) 114, 159
BmiI GGNNCC 2 cut(s) 116, 241
BmrFI CCNGG 1 cut(s) 13
BmsI GCATC 3 cut(s) 160, 217, 497
BmtI GCTAGC 1 cut(s) 380
BsaJI CCNNGG 4 cut(s) 11, 37, 231, 469
BseBI CCWGG 1 cut(s) 13
BseDI CCNNGG 4 cut(s) 11, 37, 231, 469
BseGI GGATG 4 cut(s) 7, 175, 232, 268
BseMII CTCAG 2 cut(s) 492, 506
BseXI GCAGC 3 cut(s) 73, 382, 448
BseYI CCCAGC 1 cut(s) 81
Bsh1236I CGCG 1 cut(s) 106
BshFI GGCC 2 cut(s) 153, 332
BsiHKAI GWGCWC 1 cut(s) 46
BsmAI GTCTC 2 cut(s) 10, 177
BsmBI CGTCTC 1 cut(s) 177
BsnI GGCC 2 cut(s) 153, 332
Bsp1286I GDGCHC 1 cut(s) 46
Bsp143I GATC 1 cut(s) 239
BspACI CCGC 3 cut(s) 106, 218, 236
BspANI GGCC 2 cut(s) 153, 332
BspCNI CTCAG 2 cut(s) 493, 505
BspFNI CGCG 1 cut(s) 106
BspLI GGNNCC 2 cut(s) 116, 241
BspMAI CTGCAG 1 cut(s) 400
BspOI GCTAGC 1 cut(s) 380
BspPI GGATC 2 cut(s) 234, 247
BssECI CCNNGG 4 cut(s) 11, 37, 231, 469
BssMI GATC 1 cut(s) 239
BssT1I CCWWGG 3 cut(s) 37, 231, 469
Bst2UI CCWGG 1 cut(s) 13
Bst4CI ACNGT 1 cut(s) 133
BstC8I GCNNGC 2 cut(s) 374, 378
BstDEI CTNAG 2 cut(s) 492, 501
BstF5I GGATG 4 cut(s) 7, 175, 232, 268
BstFNI CGCG 1 cut(s) 106
BstHHI GCGC 1 cut(s) 6
BstKTI GATC 1 cut(s) 242
BstMAI GTCTC 2 cut(s) 10, 177
BstMBI GATC 1 cut(s) 239
BstNI CCWGG 1 cut(s) 13
BstSCI CCNGG 1 cut(s) 11
BstSFI CTRYAG 1 cut(s) 396
BstUI CGCG 1 cut(s) 106
BstV1I GCAGC 3 cut(s) 73, 382, 448
BstX2I RGATCY 1 cut(s) 239
BstYI RGATCY 1 cut(s) 239
BsuRI GGCC 2 cut(s) 153, 332
BtsCI GGATG 4 cut(s) 7, 175, 232, 268
BtsI GCAGTG 1 cut(s) 456
BtsIMutI CAGTG 2 cut(s) 138, 456
Cac8I GCNNGC 2 cut(s) 374, 378
CfoI GCGC 1 cut(s) 6
Cfr13I GGNCC 2 cut(s) 114, 159
Csp6I GTAC 1 cut(s) 453
CviAII CATG 1 cut(s) 111
CviQI GTAC 1 cut(s) 453
DdeI CTNAG 2 cut(s) 492, 501
DpnI GATC 1 cut(s) 241
DpnII GATC 1 cut(s) 239
DraI TTTAAA 1 cut(s) 327
Ecl136II GAGCTC 1 cut(s) 44
Eco130I CCWWGG 3 cut(s) 37, 231, 469
Eco147I AGGCCT 1 cut(s) 332
Eco24I GRGCYC 1 cut(s) 46
Eco32I GATATC 1 cut(s) 498
Eco47I GGWCC 2 cut(s) 114, 159
Eco53kI GAGCTC 1 cut(s) 44
EcoICRI GAGCTC 1 cut(s) 44
EcoRII CCWGG 1 cut(s) 11
EcoRV GATATC 1 cut(s) 498
EcoT14I CCWWGG 3 cut(s) 37, 231, 469
EcoT38I GRGCYC 1 cut(s) 46
ErhI CCWWGG 3 cut(s) 37, 231, 469
Esp3I CGTCTC 1 cut(s) 177
FaeI CATG 1 cut(s) 114
FaiI YATR 5 cut(s) 112, 125, 157, 166, 437
FatI CATG 1 cut(s) 110
FauI CCCGC 1 cut(s) 229
Fnu4HI GCNGC 3 cut(s) 87, 396, 462
FokI GGATG 3 cut(s) 182, 239, 275
FriOI GRGCYC 1 cut(s) 46
Fsp4HI GCNGC 3 cut(s) 87, 396, 462
FspBI CTAG 2 cut(s) 38, 377
GlaI GCGC 1 cut(s) 5
GluI GCNGC 3 cut(s) 87, 396, 462
GsaI CCCAGC 1 cut(s) 85
HaeIII GGCC 2 cut(s) 153, 332
HhaI GCGC 1 cut(s) 6
Hin1II CATG 1 cut(s) 114
Hin6I GCGC 1 cut(s) 4
HinP1I GCGC 1 cut(s) 4
HinfI GANTC 4 cut(s) 272, 286, 432, 549
HphI GGTGA 4 cut(s) 203, 306, 365, 374
Hpy166II GTNNAC 1 cut(s) 453
Hpy188I TCNGA 3 cut(s) 495, 502, 548
Hpy8I GTNNAC 1 cut(s) 453
HpyAV CCTTC 1 cut(s) 568
HpyCH4III ACNGT 1 cut(s) 133
HpyCH4V TGCA 4 cut(s) 206, 304, 398, 515
HpyF3I CTNAG 2 cut(s) 492, 501
Hsp92II CATG 1 cut(s) 114
HspAI GCGC 1 cut(s) 4
Kzo9I GATC 1 cut(s) 239
LmnI GCTCC 2 cut(s) 41, 94
LpnPI CCDG 5 cut(s) 15, 25, 67, 386, 507
Lsp1109I GCAGC 3 cut(s) 73, 382, 448
LweI GCATC 3 cut(s) 160, 217, 497
MaeI CTAG 2 cut(s) 38, 377
MaeIII GTNAC 1 cut(s) 362
MalI GATC 1 cut(s) 241
MboI GATC 1 cut(s) 239
MboII GAAGA 1 cut(s) 301
MflI RGATCY 1 cut(s) 239
MhlI GDGCHC 1 cut(s) 46
MluCI AATT 5 cut(s) 48, 94, 221, 305, 507
MseI TTAA 1 cut(s) 326
MspR9I CCNGG 1 cut(s) 13
MvaI CCWGG 1 cut(s) 13
MvnI CGCG 1 cut(s) 106
NdeII GATC 1 cut(s) 239
NheI GCTAGC 1 cut(s) 376
NlaIII CATG 1 cut(s) 114
NlaIV GGNNCC 2 cut(s) 116, 241
NmuCI GTSAC 1 cut(s) 362
PceI AGGCCT 1 cut(s) 332
PfeI GAWTC 4 cut(s) 272, 286, 432, 549
PkrI GCNGC 3 cut(s) 88, 397, 463
Psp124BI GAGCTC 1 cut(s) 46
Psp6I CCWGG 1 cut(s) 11
PspFI CCCAGC 1 cut(s) 81
PspGI CCWGG 1 cut(s) 11
PspN4I GGNNCC 2 cut(s) 116, 241
PspPI GGNCC 2 cut(s) 114, 159
PstI CTGCAG 1 cut(s) 400
PsuI RGATCY 1 cut(s) 239
RsaI GTAC 1 cut(s) 454
RsaNI GTAC 1 cut(s) 453
SacI GAGCTC 1 cut(s) 46
SaqAI TTAA 1 cut(s) 326
SatI GCNGC 3 cut(s) 87, 396, 462
Sau3AI GATC 1 cut(s) 239
Sau96I GGNCC 2 cut(s) 114, 159
ScrFI CCNGG 1 cut(s) 13
SduI GDGCHC 1 cut(s) 46
SetI ASST 6 cut(s) 46, 378, 382, 418, 485, 526
SfaNI GCATC 3 cut(s) 160, 217, 497
SfcI CTRYAG 1 cut(s) 396
SinI GGWCC 2 cut(s) 114, 159
Sse9I AATT 5 cut(s) 48, 94, 221, 305, 507
SseBI AGGCCT 1 cut(s) 332
SsiI CCGC 3 cut(s) 106, 218, 236
SspMI CTAG 2 cut(s) 38, 377
SstI GAGCTC 1 cut(s) 46
StuI AGGCCT 1 cut(s) 332
StyD4I CCNGG 1 cut(s) 11
StyI CCWWGG 3 cut(s) 37, 231, 469
TaaI ACNGT 1 cut(s) 133
TasI AATT 5 cut(s) 48, 94, 221, 305, 507
TfiI GAWTC 4 cut(s) 272, 286, 432, 549
Tru1I TTAA 1 cut(s) 326
Tru9I TTAA 1 cut(s) 326
TscAI CASTG 2 cut(s) 138, 463
TseFI GTSAC 1 cut(s) 362
TseI GCWGC 3 cut(s) 86, 395, 461
Tsp45I GTSAC 1 cut(s) 362
TspDTI ATGAA 5 cut(s) 213, 282, 285, 417, 526
TspRI CASTG 2 cut(s) 138, 463
VpaK11BI GGWCC 2 cut(s) 114, 159
XapI RAATTY 1 cut(s) 94
XmaJI CCTAGG 1 cut(s) 37
XspI CTAG 2 cut(s) 38, 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.