MD16G1098000.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013258
Physical Location & Seq
Reverse (-)
6864287 .. 6865303
1017 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1098000.v1.1.491

Sequence Viewer

Length: 876 bp
ATGCGGGCACTGGCTGCTGTTAATGATATTTCTGGGGCTGAAAGGGTTATTGATGAGATGAAGAGGGATGGTCGAGTTGCCAGTGATTGGACCACGTATAGCAACTTAGCTTCAATTTTTGTTGAAGCTGGCATGTTTGAGAAGGCAGAAAATGCGCTTAAAGAATTGGAGAAGAAAAATTTGTGCCGAGATCTTTCGGCTTTCCAATTCCTCATTACATTGTATGGTAAAACAGGGAACCTGCGTGAAATTTATCGGGTATGGCGTTCCTTGAGGCTGGCTTTCCCTAAAACTGCAAATATCAGCTATCTGAATATGATCCAGGTGTTGGTCAACTTGAATGATGTCCCAGGTGCAGAGAAAACTTTCAGAGAATGGGAATGCCAGTGCTCAACCTATGATATTCGGGTAGCAAATGTTTTGATTGGAGCTTATGCTGAACAGGGTATGCTAGAGAAGGCCAAGGAACTCAAGGAGCTGGCCCGCAGGAGAGGAGCCAAGCCTAATGCAAAAACCTGGGAGATCTTTCTGGATTATTATCTGAAGTCTGGAGAATATCAATTGGCGGTTGATTGTGTTGCCAATGCAATATCCATTGGTAGGGGGGATGGTAGGAAGTGGAGTCCATCACATAAAATTGTCAAAACTTTGATGGAGAATTTCGAGCAAGAGAAGGATGTTGATGGTGCAGAAGGTTTTCTGGAGATTCTGAAGAAGTCCGTAGAATCCTTAGGGCTTGAGGTGTTTGAATCATTGATCAGAACTTATGCTGCTGCTGGAAGGACAAGTCCTGCTATGCGTGCTCGTTTGAAAATGGAGAAATTGGAGGTGAGTGAAGCCAGTGAAAAGTTGCTCGAGGCCGTATGTGTGGAATGA

Protein Analysis

292

Amino Acids

32.89

Weight (kDa)

6.7

Isoelectric Point (pI)

26.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 137 - 179 3.1e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013258)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60770
fragaria_vesca FvH4_4g12450
malus_domestica MD13G1097100.v1.1 MD16G1098000.v1.1
prunus_persica Prupe.1G248500_v2.0.a1
pyrus_communis pycom13g08480 pycom16g08350
rosa_chinensis RchiOBHm_Chr4g0410731
rosa_laevigata RLG00000008406
rosa_multiflora Rmu_sc0001967.1_g000008 Rmu_ssc0000072.1_g000002
rosa_roxburghii Rroxscaffold_5G00355480
rosa_rugosa Rorug04G0111500
rosa_samantha Rh4AG162600 Rh4BG161400 Rh4CG175500 Rh4DG158200
rosa_wichuraiana Rw4G013540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 249
AccB7I CCANNNNNTGG 2 cut(s) 87, 328
AciI CCGC 3 cut(s) 4, 484, 566
AclWI GGATC 1 cut(s) 313
AcsI RAATTY 3 cut(s) 178, 249, 658
AcuI CTGAAG 2 cut(s) 563, 731
AfiI CCNNNNNNNGG 3 cut(s) 87, 328, 600
AgsI TTSAA 5 cut(s) 114, 125, 340, 749, 811
AjnI CCWGG 3 cut(s) 321, 349, 515
AluBI AGCT 5 cut(s) 110, 128, 306, 431, 478
AluI AGCT 5 cut(s) 110, 128, 306, 431, 478
Alw21I GWGCWC 2 cut(s) 392, 805
AlwI GGATC 1 cut(s) 313
Ama87I CYCGRG 1 cut(s) 854
AoxI GGCC 3 cut(s) 459, 480, 858
ApeKI GCWGC 3 cut(s) 14, 770, 773
ApoI RAATTY 3 cut(s) 178, 249, 658
Asp700I GAANNNNTTC 2 cut(s) 365, 696
AspLEI GCGC 1 cut(s) 157
AspS9I GGNCC 2 cut(s) 90, 481
AsuHPI GGTGA 1 cut(s) 841
AvaI CYCGRG 1 cut(s) 854
AvaII GGWCC 1 cut(s) 90
AxyI CCTNAGG 1 cut(s) 730
BaeGI GKGCMC 1 cut(s) 10
Bbv12I GWGCWC 2 cut(s) 392, 805
BbvI GCAGC 2 cut(s) 757, 760
BccI CCATC 5 cut(s) 62, 602, 634, 646, 677
BceAI ACGGC 1 cut(s) 845
BciT130I CCWGG 3 cut(s) 323, 351, 517
BclI TGATCA 1 cut(s) 756
BfaI CTAG 1 cut(s) 452
BfuAI ACCTGC 1 cut(s) 249
BglII AGATCT 2 cut(s) 190, 522
BisI GCNGC 3 cut(s) 15, 771, 774
BlsI GCNGC 3 cut(s) 16, 772, 775
Bme1390I CCNGG 3 cut(s) 323, 351, 517
Bme18I GGWCC 1 cut(s) 90
BmeT110I CYCGRG 1 cut(s) 854
BmgT120I GGNCC 2 cut(s) 90, 481
BmiI GGNNCC 2 cut(s) 239, 496
BmrFI CCNGG 3 cut(s) 323, 351, 517
BpmI CTGGAG 2 cut(s) 570, 722
BpuEI CTTGAG 3 cut(s) 292, 455, 758
BsaAI YACGTR 1 cut(s) 96
BsaBI GATNNNNATC 1 cut(s) 537
BsaJI CCNNGG 3 cut(s) 349, 462, 516
Bsc4I CCNNNNNNNGG 3 cut(s) 87, 328, 600
Bse1I ACTGG 4 cut(s) 15, 81, 385, 840
Bse21I CCTNAGG 1 cut(s) 730
Bse8I GATNNNNATC 1 cut(s) 537
BseBI CCWGG 3 cut(s) 323, 351, 517
BseDI CCNNGG 3 cut(s) 349, 462, 516
BseGI GGATG 3 cut(s) 73, 613, 682
BseJI GATNNNNATC 1 cut(s) 537
BseLI CCNNNNNNNGG 3 cut(s) 87, 328, 600
BseNI ACTGG 4 cut(s) 15, 81, 385, 840
BseRI GAGGAG 1 cut(s) 507
BseSI GKGCMC 1 cut(s) 10
BseXI GCAGC 2 cut(s) 757, 760
BsgI GTGCAG 2 cut(s) 375, 708
BshFI GGCC 3 cut(s) 461, 482, 860
BsiHKAI GWGCWC 2 cut(s) 392, 805
BsiHKCI CYCGRG 1 cut(s) 854
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 3 cut(s) 87, 328, 600
BsmFI GGGAC 1 cut(s) 332
BsmI GAATGC 1 cut(s) 386
BsnI GGCC 3 cut(s) 461, 482, 860
BsoBI CYCGRG 1 cut(s) 854
Bsp1286I GDGCHC 3 cut(s) 10, 392, 805
Bsp143I GATC 4 cut(s) 190, 318, 522, 756
BspACI CCGC 3 cut(s) 4, 484, 566
BspANI GGCC 3 cut(s) 461, 482, 860
BspLI GGNNCC 2 cut(s) 239, 496
BspMI ACCTGC 1 cut(s) 249
BspPI GGATC 1 cut(s) 313
BsrI ACTGG 4 cut(s) 15, 81, 385, 840
BssECI CCNNGG 3 cut(s) 349, 462, 516
BssMI GATC 4 cut(s) 190, 318, 522, 756
BssT1I CCWWGG 1 cut(s) 462
Bst2UI CCWGG 3 cut(s) 323, 351, 517
Bst6I CTCTTC 1 cut(s) 56
BstAPI GCANNNNNTGC 2 cut(s) 14, 152
BstBAI YACGTR 1 cut(s) 96
BstC8I GCNNGC 6 cut(s) 6, 130, 279, 480, 484, 801
BstDEI CTNAG 2 cut(s) 106, 730
BstF5I GGATG 3 cut(s) 73, 613, 682
BstHHI GCGC 1 cut(s) 157
BstKTI GATC 4 cut(s) 193, 321, 525, 759
BstMBI GATC 4 cut(s) 190, 318, 522, 756
BstMWI GCNNNNNNNGC 3 cut(s) 14, 152, 800
BstNI CCWGG 3 cut(s) 323, 351, 517
BstNSI RCATGY 1 cut(s) 136
BstSCI CCNGG 3 cut(s) 321, 349, 515
BstSLI GKGCMC 1 cut(s) 10
BstV1I GCAGC 2 cut(s) 757, 760
BstX2I RGATCY 2 cut(s) 190, 522
BstYI RGATCY 2 cut(s) 190, 522
Bsu36I CCTNAGG 1 cut(s) 730
BsuRI GGCC 3 cut(s) 461, 482, 860
BtsCI GGATG 3 cut(s) 73, 613, 682
BtsIMutI CAGTG 4 cut(s) 8, 88, 392, 847
BveI ACCTGC 1 cut(s) 249
Cac8I GCNNGC 6 cut(s) 6, 130, 279, 480, 484, 801
CfoI GCGC 1 cut(s) 157
Cfr13I GGNCC 2 cut(s) 90, 481
CviAII CATG 1 cut(s) 133
DdeI CTNAG 2 cut(s) 106, 730
DpnI GATC 4 cut(s) 192, 320, 524, 758
DpnII GATC 4 cut(s) 190, 318, 522, 756
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Eco130I CCWWGG 1 cut(s) 462
Eco47I GGWCC 1 cut(s) 90
Eco57I CTGAAG 2 cut(s) 563, 731
Eco81I CCTNAGG 1 cut(s) 730
Eco88I CYCGRG 1 cut(s) 854
EcoRII CCWGG 3 cut(s) 321, 349, 515
EcoT14I CCWWGG 1 cut(s) 462
ErhI CCWWGG 1 cut(s) 462
FaeI CATG 1 cut(s) 136
FaqI GGGAC 1 cut(s) 332
FatI CATG 1 cut(s) 132
FauI CCCGC 1 cut(s) 491
FbaI TGATCA 1 cut(s) 756
Fnu4HI GCNGC 3 cut(s) 15, 771, 774
FokI GGATG 3 cut(s) 80, 620, 689
Fsp4HI GCNGC 3 cut(s) 15, 771, 774
FspBI CTAG 1 cut(s) 452
GlaI GCGC 1 cut(s) 156
GluI GCNGC 3 cut(s) 15, 771, 774
GsuI CTGGAG 2 cut(s) 570, 722
HaeIII GGCC 3 cut(s) 461, 482, 860
HhaI GCGC 1 cut(s) 157
Hin1II CATG 1 cut(s) 136
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HinfI GANTC 4 cut(s) 622, 706, 725, 749
HphI GGTGA 1 cut(s) 841
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 5 cut(s) 312, 371, 543, 711, 761
Hpy188III TCNNGA 3 cut(s) 530, 549, 701
Hpy8I GTNNAC 1 cut(s) 334
HpyAV CCTTC 5 cut(s) 136, 451, 667, 686, 774
HpyCH4IV ACGT 1 cut(s) 95
HpyCH4V TGCA 5 cut(s) 296, 356, 509, 587, 689
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 152, 800
HpyF3I CTNAG 2 cut(s) 106, 730
HpySE526I ACGT 1 cut(s) 95
Hsp92II CATG 1 cut(s) 136
HspAI GCGC 1 cut(s) 155
Ksp22I TGATCA 1 cut(s) 756
Kzo9I GATC 4 cut(s) 190, 318, 522, 756
LmnI GCTCC 3 cut(s) 428, 475, 494
Lsp1109I GCAGC 2 cut(s) 757, 760
MaeI CTAG 1 cut(s) 452
MaeII ACGT 1 cut(s) 95
MalI GATC 4 cut(s) 192, 320, 524, 758
MboI GATC 4 cut(s) 190, 318, 522, 756
MboII GAAGA 3 cut(s) 73, 184, 724
MfeI CAATTG 1 cut(s) 560
MflI RGATCY 2 cut(s) 190, 522
MhlI GDGCHC 3 cut(s) 10, 392, 805
MluCI AATT 9 cut(s) 114, 164, 178, 206, 249, 560, 636, 658, 821
MlyI GAGTC 1 cut(s) 631
MnlI CCTC 7 cut(s) 57, 221, 267, 485, 733, 820, 850
MroXI GAANNNNTTC 2 cut(s) 365, 696
MseI TTAA 2 cut(s) 21, 159
MspR9I CCNGG 3 cut(s) 323, 351, 517
MunI CAATTG 1 cut(s) 560
Mva1269I GAATGC 1 cut(s) 386
MvaI CCWGG 3 cut(s) 323, 351, 517
MwoI GCNNNNNNNGC 3 cut(s) 14, 152, 800
NdeII GATC 4 cut(s) 190, 318, 522, 756
NlaIII CATG 1 cut(s) 136
NlaIV GGNNCC 2 cut(s) 239, 496
NmeAIII GCCGAG 1 cut(s) 212
NspI RCATGY 1 cut(s) 136
PaeR7I CTCGAG 1 cut(s) 854
PcsI WCGNNNNNNNCGW 1 cut(s) 262
PctI GAATGC 1 cut(s) 386
PdmI GAANNNNTTC 2 cut(s) 365, 696
PfeI GAWTC 3 cut(s) 706, 725, 749
PflMI CCANNNNNTGG 2 cut(s) 87, 328
PkrI GCNGC 3 cut(s) 16, 772, 775
PleI GAGTC 1 cut(s) 630
PpsI GAGTC 1 cut(s) 630
Ppu21I YACGTR 1 cut(s) 96
Psp6I CCWGG 3 cut(s) 321, 349, 515
PspGI CCWGG 3 cut(s) 321, 349, 515
PspN4I GGNNCC 2 cut(s) 239, 496
PspPI GGNCC 2 cut(s) 90, 481
PspXI VCTCGAGB 1 cut(s) 854
PsuI RGATCY 2 cut(s) 190, 522
SaqAI TTAA 2 cut(s) 21, 159
SatI GCNGC 3 cut(s) 15, 771, 774
Sau3AI GATC 4 cut(s) 190, 318, 522, 756
Sau96I GGNCC 2 cut(s) 90, 481
SchI GAGTC 1 cut(s) 631
ScrFI CCNGG 3 cut(s) 323, 351, 517
SduI GDGCHC 3 cut(s) 10, 392, 805
Sfr274I CTCGAG 1 cut(s) 854
SinI GGWCC 1 cut(s) 90
SlaI CTCGAG 1 cut(s) 854
SmlI CTYRAG 4 cut(s) 271, 470, 737, 854
SmoI CTYRAG 4 cut(s) 271, 470, 737, 854
Sse9I AATT 9 cut(s) 114, 164, 178, 206, 249, 560, 636, 658, 821
SsiI CCGC 3 cut(s) 4, 484, 566
SspMI CTAG 1 cut(s) 452
StyD4I CCNGG 3 cut(s) 321, 349, 515
StyI CCWWGG 1 cut(s) 462
TaiI ACGT 1 cut(s) 98
TaqI TCGA 3 cut(s) 73, 663, 855
TasI AATT 9 cut(s) 114, 164, 178, 206, 249, 560, 636, 658, 821
TfiI GAWTC 3 cut(s) 706, 725, 749
Tru1I TTAA 2 cut(s) 21, 159
Tru9I TTAA 2 cut(s) 21, 159
TscAI CASTG 4 cut(s) 15, 88, 392, 847
TseI GCWGC 3 cut(s) 14, 770, 773
TspDTI ATGAA 1 cut(s) 74
TspGWI ACGGA 1 cut(s) 709
TspRI CASTG 4 cut(s) 15, 88, 392, 847
Van91I CCANNNNNTGG 2 cut(s) 87, 328
VpaK11BI GGWCC 1 cut(s) 90
XapI RAATTY 3 cut(s) 178, 249, 658
XceI RCATGY 1 cut(s) 136
XhoI CTCGAG 1 cut(s) 854
XmnI GAANNNNTTC 2 cut(s) 365, 696
XspI CTAG 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.